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The map label for this gene is trpB

Identifier: 121637519

GI number: 121637519

Start: 1816374

End: 1817606

Strand: Direct

Name: trpB

Synonym: BCG_1650

Alternate gene names: 121637519

Gene position: 1816374-1817606 (Clockwise)

Preceding gene: 121637518

Following gene: 121637520

Centisome position: 41.52

GC content: 62.77

Gene sequence:

>1233_bases
ATGAGTGCTGCCATCGCCGAACCGACCAGTCACGATCCTGATTCCGGCGGCCATTTCGGCGGCCCCAGTGGTTGGGGTGG
CCGCTACGTTCCCGAGGCGCTGATGGCGGTGATCGAAGAGGTCACCGCCGCCTACCAAAAGGAGCGCGTCAGCCAGGACT
TTCTGGACGACCTAGACAGGCTGCAGGCGAACTATGCGGGCCGGCCTTCGCCGCTTTACGAGGCGACCCGGTTGAGCCAG
CACGCTGGGTCGGCGCGAATCTTTCTGAAGCGAGAAGACCTGAACCATACTGGTTCTCACAAGATCAACAACGTGCTCGG
GCAGGCACTGCTGGCGCGCAGGATGGGCAAGACCCGGGTGATCGCCGAGACCGGTGCCGGCCAGCACGGGGTCGCCACGG
CCACCGCATGCGCATTGCTCGGCCTGGACTGTGTCATCTACATGGGGGGCATCGACACCGCCCGTCAGGCGCTAAACGTG
GCCCGGATGCGATTGCTGGGTGCCGAAGTCGTCGCGGTTCAGACGGGCTCGAAAACGCTCAAAGACGCCATCAATGAGGC
GTTCCGGGATTGGGTTGCCAACGCCGACAACACCTACTACTGCTTTGGTACTGCGGCCGGACCGCATCCGTTTCCAACCA
TGGTGCGCGATTTCCAGCGAATCATCGGCATGGAGGCACGTGTGCAGATCCAGGGTCAGGCCGGTCGGCTGCCTGACGCC
GTCGTCGCGTGCGTTGGTGGCGGGTCCAATGCCATTGGTATTTTTCATGCGTTTCTCGATGACCCAGGCGTACGGCTGGT
CGGATTCGAGGCAGCCGGCGACGGCGTTGAGACCGGCCGGCATGCCGCGACATTCACCGCTGGTTCGCCCGGGGCATTTC
ACGGATCGTTCTCGTACTTGCTGCAAGACGAGGACGGTCAGACCATTGAATCCCATTCAATTTCCGCGGGTCTGGATTAT
CCGGGGGTGGGCCCGGAACATGCGTGGCTCAAGGAGGCCGGGCGTGTCGATTATCGGCCGATCACCGACTCCGAGGCGAT
GGACGCGTTTGGCCTGCTGTGTCGCATGGAAGGCATCATCCCGGCTATTGAATCCGCGCACGCGGTGGCCGGCGCCCTCA
AGCTAGGTGTTGAGTTGGGAAGGGGCGCGGTGATTGTGGTGAACCTGTCGGGACGTGGCGACAAAGATGTCGAGACGGCC
GCGAAATGGTTTGGCTTGCTGGGCAACGACTGA

Upstream 100 bases:

>100_bases
CCCATCCGTCCTGTCCGAAACCGGCTCGCTAGCCGTCGATGAGCCGCTTGCATCTTGAGCCTCGGTGATGACAGATCTAT
CCACCCCGGATCTTCCGCGC

Downstream 100 bases:

>100_bases
TGGTGGCGGTGGAACAGAGCGAAGCAAGTAGGCTCGGGCCGGTTTTCGATTCCTGCCGTGCAAACAACCGCGCGGCATTG
ATTGGTTACTTGCCGACCGG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSAAIAEPTSHDPDSGGHFGGPSGWGGRYVPEALMAVIEEVTAAYQKERVSQDFLDDLDRLQANYAGRPSPLYEATRLSQ
HAGSARIFLKREDLNHTGSHKINNVLGQALLARRMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGGIDTARQALNV
ARMRLLGAEVVAVQTGSKTLKDAINEAFRDWVANADNTYYCFGTAAGPHPFPTMVRDFQRIIGMEARVQIQGQAGRLPDA
VVACVGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATFTAGSPGAFHGSFSYLLQDEDGQTIESHSISAGLDY
PGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGIIPAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETA
AKWFGLLGND

Sequences:

>Translated_410_residues
MSAAIAEPTSHDPDSGGHFGGPSGWGGRYVPEALMAVIEEVTAAYQKERVSQDFLDDLDRLQANYAGRPSPLYEATRLSQ
HAGSARIFLKREDLNHTGSHKINNVLGQALLARRMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGGIDTARQALNV
ARMRLLGAEVVAVQTGSKTLKDAINEAFRDWVANADNTYYCFGTAAGPHPFPTMVRDFQRIIGMEARVQIQGQAGRLPDA
VVACVGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATFTAGSPGAFHGSFSYLLQDEDGQTIESHSISAGLDY
PGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGIIPAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETA
AKWFGLLGND
>Mature_409_residues
SAAIAEPTSHDPDSGGHFGGPSGWGGRYVPEALMAVIEEVTAAYQKERVSQDFLDDLDRLQANYAGRPSPLYEATRLSQH
AGSARIFLKREDLNHTGSHKINNVLGQALLARRMGKTRVIAETGAGQHGVATATACALLGLDCVIYMGGIDTARQALNVA
RMRLLGAEVVAVQTGSKTLKDAINEAFRDWVANADNTYYCFGTAAGPHPFPTMVRDFQRIIGMEARVQIQGQAGRLPDAV
VACVGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATFTAGSPGAFHGSFSYLLQDEDGQTIESHSISAGLDYP
GVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGIIPAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETAA
KWFGLLGND

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=376, Percent_Identity=55.3191489361702, Blast_Score=417, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=56.8831168831169, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_MYCBO (P66985)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855291.1
- ProteinModelPortal:   P66985
- SMR:   P66985
- EnsemblBacteria:   EBMYCT00000015113
- GeneID:   1092570
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1638
- GeneTree:   EBGT00050000015765
- HOGENOM:   HBG303148
- ProtClustDB:   PRK04346
- BioCyc:   MBOV233413:MB1638-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43287; Mature: 43156

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIAEPTSHDPDSGGHFGGPSGWGGRYVPEALMAVIEEVTAAYQKERVSQDFLDDLDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQANYAGRPSPLYEATRLSQHAGSARIFLKREDLNHTGSHKINNVLGQALLARRMGKTRV
HHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IAETGAGQHGVATATACALLGLDCVIYMGGIDTARQALNVARMRLLGAEVVAVQTGSKTL
EEECCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCHHH
KDAINEAFRDWVANADNTYYCFGTAAGPHPFPTMVRDFQRIIGMEARVQIQGQAGRLPDA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHH
VVACVGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATFTAGSPGAFHGSFSYL
HEEEECCCCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEE
LQDEDGQTIESHSISAGLDYPGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGII
EECCCCCEEHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETAAKWFGLLGND
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAAIAEPTSHDPDSGGHFGGPSGWGGRYVPEALMAVIEEVTAAYQKERVSQDFLDDLDR
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQANYAGRPSPLYEATRLSQHAGSARIFLKREDLNHTGSHKINNVLGQALLARRMGKTRV
HHHHCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IAETGAGQHGVATATACALLGLDCVIYMGGIDTARQALNVARMRLLGAEVVAVQTGSKTL
EEECCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCEEEEEECCCHHH
KDAINEAFRDWVANADNTYYCFGTAAGPHPFPTMVRDFQRIIGMEARVQIQGQAGRLPDA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHH
VVACVGGGSNAIGIFHAFLDDPGVRLVGFEAAGDGVETGRHAATFTAGSPGAFHGSFSYL
HEEEECCCCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEE
LQDEDGQTIESHSISAGLDYPGVGPEHAWLKEAGRVDYRPITDSEAMDAFGLLCRMEGII
EECCCCCEEHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PAIESAHAVAGALKLGVELGRGAVIVVNLSGRGDKDVETAAKWFGLLGND
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972