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The map label for this gene is metE

Identifier: 121637065

GI number: 121637065

Start: 1290482

End: 1292761

Strand: Reverse

Name: metE

Synonym: BCG_1194c

Alternate gene names: 121637065

Gene position: 1292761-1290482 (Counterclockwise)

Preceding gene: 121637067

Following gene: 121637061

Centisome position: 29.55

GC content: 69.12

Gene sequence:

>2280_bases
GTGACCCAGCCTGTACGTCGTCAACCCTTTACCGCAACCATCACCGGCTCCCCGCGCATCGGCCCGCGCCGCGAACTCAA
GCGCGCCACCGAAGGCTACTGGGCCGGACGTACCAGCCGATCCGAGCTGGAGGCCGTCGCCGCCACGTTACGCCGCGACA
CCTGGTCGGCCCTGGCCGCGGCCGGTCTGGACTCGGTGCCGGTGAACACCTTCTCCTACTACGACCAAATGCTCGATACC
GCGGTGCTGCTCGGCGCGCTGCCGCCCCGAGTGAGCCCGGTTTCCGACGGGCTGGACCGCTATTTCGCCGCGGCGCGGGG
CACCGACCAGATCGCGCCGCTGGAGATGACGAAGTGGTTCGACACCAACTACCACTACCTGGTACCCGAGATCGGGCCGT
CGACCACGTTCACGCTGCACCCCGGCAAGGTGCTCGCCGAACTCAAAGAGGCGTTAGGGCAAGGCATTCCCGCACGTCCG
GTGATCATCGGGCCGATCACCTTCCTGCTGCTGAGCAAGGCCGTCGACGGCGCGGGGGCGCCGATCGAACGCCTCGAAGA
GTTGGTTCCGGTCTATTCGGAGCTGCTGTCGCTGCTTGCCGACGGCGGCGCCCAGTGGGTGCAGTTCGACGAGCCGGCGC
TGGTGACCGACCTCTCCCCCGACGCGCCCGCCCTGGCTGAAGCGGTGTACACCGCGCTGTGCTCGGTGAGCAACCGGCCT
GCGATCTATGTCGCCACCTACTTCGGGGACCCGGGCGCGGCCCTACCGGCGCTGGCTCGCACCCCGGTCGAAGCCATCGG
CGTCGACCTAGTGGCCGGTGCCGACACCTCGGTGGCCGGGGTACCCGAGCTGGCCGGCAAGACGCTGGTGGCCGGGGTCG
TCGACGGGCGCAACGTCTGGCGCACCGACCTGGAGGCGGCGTTGGGCACGTTGGCGACCCTGCTGGGTTCGGCGGCTACC
GTGGCCGTCTCGACGTCGTGCTCGACACTGCACGTGCCGTACTCGCTGGAACCGGAAACCGACCTGGATGACGCGTTGCG
GAGCTGGCTGGCGTTCGGTGCCGAAAAGGTGCGCGAAGTCGTCGTTCTCGCGCGTGCCCTGCGCGACGGACACGACGCGG
TCGCCGACGAGATCGCGTCGTCCCGCGCCGCCATCGCGTCCCGCAAGCGCGACCCGCGGTTACACAATGGGCAAATCCGG
GCGCGCATCGAGGCGATCGTCGCGTCCGGAGCCCACCGCGGCAATGCCGCCCAGCGCCGCGCCAGCCAAGACGCGCGACT
GCACCTGCCGCCGCTGCCGACCACGACGATCGGCTCCTACCCGCAGACCTCGGCGATCCGCGTTGCGCGTGCGGCGCTGC
GGGCCGGTGAGATCGACGAGGCCGAGTACGTGCGCCGGATGCGGCAAGAGATCACCGAGGTGATCGCGCTACAGGAGCGG
CTCGGGCTCGACGTGCTGGTGCACGGCGAACCGGAGCGCAACGACATGGTGCAGTACTTCGCCGAGCAATTGGCGGGTTT
CTTCGCTACCCAGAACGGCTGGGTGCAGTCCTACGGCAGCCGCTGTGTGCGTCCGCCGATCCTGTACGGCGACGTGTCCC
GGCCGCGGGCGATGACGGTCGAGTGGATCACCTACGCGCAGTCGCTGACCGACAAACCGGTGAAGGGCATGTTGACCGGG
CCGGTGACGATTCTGGCGTGGTCGTTCGTGCGTGACGACCAGCCGTTGGCCGATACCGCCAACCAGGTGGCGCTGGCGAT
TCGCGACGAGACCGTGGATTTGCAGTCCGCCGGCATCGCGGTCATCCAGGTCGACGAGCCTGCGCTGCGTGAACTGCTGC
CGCTGCGTCGCGCCGACCAGGCCGAGTACTTGCGTTGGGCGGTAGGGGCTTTCCGGTTGGCCACCTCCGGCGTCTCGGAC
GCCACCCAGATCCACACGCATCTGTGCTACTCGGAGTTCGGCGAGGTGATCGGCGCGATCGCCGATCTGGACGCGGACGT
CACGTCCATCGAGGCGGCCCGGTCACACATGGAGGTGCTCGACGACCTGAACGCGATCGGCTTCGCCAACGGTGTGGGCC
CGGGCGTCTATGACATTCACTCGCCACGGGTGCCCTCCGCTGAGGAGATGGCCGACTCGTTGCGGGCCGCGTTGCGCGCG
GTGCCGGCCGAGCGGCTGTGGGTCAACCCCGACTGCGGACTGAAGACCCGCAATGTCGACGAGGTGACCGCGTCGCTGCA
CAACATGGTCGCCGCCGCCCGGGAGGTGCGCGCGGGCTAG

Upstream 100 bases:

>100_bases
CGTTTGGCCGTGCCTGTTGCCGGGTGCATCGGTGTGCACCTCAATCGGATCGGCTGCGCGTCCGCGGGCGGTGAACCACC
CGTCGATCGAAGGACAGCAC

Downstream 100 bases:

>100_bases
CTGACGAGCAGCTAGCCCGGCAGCACGTGGACCGGCACGTCGTCGTCCCAGGGCTGCCGGTTCACCATGTCGGCCACCCG
GACATAGCCGCGCTCGAACT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAVAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDT
AVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHYLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARP
VIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQWVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRP
AIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAAT
VAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIR
ARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQER
LGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTG
PVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSD
ATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRA
VPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG

Sequences:

>Translated_759_residues
MTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAVAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDT
AVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHYLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARP
VIIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQWVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRP
AIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAAT
VAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIR
ARIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQER
LGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTG
PVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSD
ATQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRA
VPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG
>Mature_758_residues
TQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAVAATLRRDTWSALAAAGLDSVPVNTFSYYDQMLDTA
VLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWFDTNYHYLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPV
IIGPITFLLLSKAVDGAGAPIERLEELVPVYSELLSLLADGGAQWVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRPA
IYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVWRTDLEAALGTLATLLGSAATV
AVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREVVVLARALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRA
RIEAIVASGAHRGNAAQRRASQDARLHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQERL
GLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGP
VTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAVIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDA
TQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRAV
PAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=753, Percent_Identity=48.074369189907, Blast_Score=684, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=45.9635416666667, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_MYCBO (P65341)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854820.1
- ProteinModelPortal:   P65341
- SMR:   P65341
- EnsemblBacteria:   EBMYCT00000014239
- GeneID:   1090458
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1164c
- GeneTree:   EBGT00050000017657
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   MBOV233413:MB1164C-MONOMER
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 81582; Mature: 81451

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAVAATLRRDTWSALAA
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLDSVPVNTFSYYDQMLDTAVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEE
DTNYHYLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPVIIGPITFLLLSKAVDGAGA
CCCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCC
PIERLEELVPVYSELLSLLADGGAQWVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRP
CHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
AIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVW
EEEEEEEECCCCCHHHHHHHCCHHHHCCEEEECCCCCCCCCHHHCCCEEEEEECCCCHHH
RTDLEAALGTLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREV
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVLARALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRR
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
ASQDARLHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQER
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCCCCEEEH
EWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEE
VIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAI
EEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ADLDADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG
CCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPVRRQPFTATITGSPRIGPRRELKRATEGYWAGRTSRSELEAVAATLRRDTWSALAA
CCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AGLDSVPVNTFSYYDQMLDTAVLLGALPPRVSPVSDGLDRYFAAARGTDQIAPLEMTKWF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEE
DTNYHYLVPEIGPSTTFTLHPGKVLAELKEALGQGIPARPVIIGPITFLLLSKAVDGAGA
CCCCEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCC
PIERLEELVPVYSELLSLLADGGAQWVQFDEPALVTDLSPDAPALAEAVYTALCSVSNRP
CHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
AIYVATYFGDPGAALPALARTPVEAIGVDLVAGADTSVAGVPELAGKTLVAGVVDGRNVW
EEEEEEEECCCCCHHHHHHHCCHHHHCCEEEECCCCCCCCCHHHCCCEEEEEECCCCHHH
RTDLEAALGTLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGAEKVREV
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVLARALRDGHDAVADEIASSRAAIASRKRDPRLHNGQIRARIEAIVASGAHRGNAAQRR
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
ASQDARLHLPPLPTTTIGSYPQTSAIRVARAALRAGEIDEAEYVRRMRQEITEVIALQER
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LGLDVLVHGEPERNDMVQYFAEQLAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTV
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEECCCCCCCEEEH
EWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEE
VIQVDEPALRELLPLRRADQAEYLRWAVGAFRLATSGVSDATQIHTHLCYSEFGEVIGAI
EEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ADLDADVTSIEAARSHMEVLDDLNAIGFANGVGPGVYDIHSPRVPSAEEMADSLRAALRA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VPAERLWVNPDCGLKTRNVDEVTASLHNMVAAAREVRAG
CCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972