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The map label for this gene is sigK

Identifier: 121636359

GI number: 121636359

Start: 564648

End: 565211

Strand: Reverse

Name: sigK

Synonym: BCG_0484c

Alternate gene names: 121636359

Gene position: 565211-564648 (Counterclockwise)

Preceding gene: 121636360

Following gene: 121636358

Centisome position: 12.92

GC content: 64.36

Gene sequence:

>564_bases
ATAACCGGACCGCCACGGCTGAGCAGCGACCTGGACGCCCTGTTGCGCCGGGTCGCTGGTCACGACCAGGCCGCATTCGC
CGAATTCTACGACCACACCAAGTCGCGGGTGTATGGACTGGTGATGCGGGTGCTGCGTGATACCGGCTACAGCGAAGAAA
CCACCCAGGAGATCTATCTTGAGGTGTGGCGGAACGCGTCGGAGTTTGACTCCGCCAAAGGTTCCGCGTTGGCCTGGCTT
TTGACCATGGCCCACCGGCGCGCTGTCGACCGAGTCCGTTGCGAGCAAGCCGGCAACCAGCGGGAAGTGCGCTATGGTGC
GGCCAACGTCGATCCCGCGAGTGACGTCGTCGCCGACCTGGCGATCGCCGGTGATGAGCGGCGCCGGGTGACCGAGTGCC
TCAAGGCGTTGACCGACACGCAGCGGCAGTGCATCGAACTGGCCTACTACGGCGGGCTGACGTATGTCGAAGTTTCGCGG
CGGTTAGCGGCCAATCTGTCGACCATCAAATCCCGCATGCGCGACGCCCTGCGCAGCCTGCGCAACTGCCTGGACGTGTC
ATGA

Upstream 100 bases:

>100_bases
AAGGCGAACAGCCTATTTCGTGCCCCGGCCACCGCGATCGGCGCGTCGGTGATGACCGGCTTGACCATCTCGGCGCCGGC
TGGCGATAGGCTACCCATCG

Downstream 100 bases:

>100_bases
CTGCGCCGGCCGAGCGAAGCGATGCGTTGGGAGAGCGGCGCTCATGACTGAACATACCGATTTTGAGCTGCTCGAGCTGG
CTACCCCGTATGCCCTGAAC

Product: RNA polymerase sigma factor SigK

Products: NA

Alternate protein names: Sigma-K factor

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTGPPRLSSDLDALLRRVAGHDQAAFAEFYDHTKSRVYGLVMRVLRDTGYSEETTQEIYLEVWRNASEFDSAKGSALAWL
LTMAHRRAVDRVRCEQAGNQREVRYGAANVDPASDVVADLAIAGDERRRVTECLKALTDTQRQCIELAYYGGLTYVEVSR
RLAANLSTIKSRMRDALRSLRNCLDVS

Sequences:

>Translated_187_residues
MTGPPRLSSDLDALLRRVAGHDQAAFAEFYDHTKSRVYGLVMRVLRDTGYSEETTQEIYLEVWRNASEFDSAKGSALAWL
LTMAHRRAVDRVRCEQAGNQREVRYGAANVDPASDVVADLAIAGDERRRVTECLKALTDTQRQCIELAYYGGLTYVEVSR
RLAANLSTIKSRMRDALRSLRNCLDVS
>Mature_186_residues
TGPPRLSSDLDALLRRVAGHDQAAFAEFYDHTKSRVYGLVMRVLRDTGYSEETTQEIYLEVWRNASEFDSAKGSALAWLL
TMAHRRAVDRVRCEQAGNQREVRYGAANVDPASDVVADLAIAGDERRRVTECLKALTDTQRQCIELAYYGGLTYVEVSRR
LAANLSTIKSRMRDALRSLRNCLDVS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SIGK_MYCBO (Q7U1Z6)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854116.1
- ProteinModelPortal:   Q7U1Z6
- SMR:   Q7U1Z6
- EnsemblBacteria:   EBMYCT00000015137
- GeneID:   1091475
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0453c
- GeneTree:   EBGT00050000015255
- HOGENOM:   HBG753452
- OMA:   AYYGGRT
- ProtClustDB:   PRK09646
- BioCyc:   MBOV233413:MB0453C-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 21035; Mature: 20904

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPPRLSSDLDALLRRVAGHDQAAFAEFYDHTKSRVYGLVMRVLRDTGYSEETTQEIYL
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVWRNASEFDSAKGSALAWLLTMAHRRAVDRVRCEQAGNQREVRYGAANVDPASDVVADL
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHH
AIAGDERRRVTECLKALTDTQRQCIELAYYGGLTYVEVSRRLAANLSTIKSRMRDALRSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNCLDVS
HHHHCCC
>Mature Secondary Structure 
TGPPRLSSDLDALLRRVAGHDQAAFAEFYDHTKSRVYGLVMRVLRDTGYSEETTQEIYL
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVWRNASEFDSAKGSALAWLLTMAHRRAVDRVRCEQAGNQREVRYGAANVDPASDVVADL
HHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHH
AIAGDERRRVTECLKALTDTQRQCIELAYYGGLTYVEVSRRLAANLSTIKSRMRDALRSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNCLDVS
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972