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The map label for this gene is hemK [H]

Identifier: 120612309

GI number: 120612309

Start: 4041958

End: 4042893

Strand: Direct

Name: hemK [H]

Synonym: Aave_3665

Alternate gene names: 120612309

Gene position: 4041958-4042893 (Clockwise)

Preceding gene: 120612308

Following gene: 120612310

Centisome position: 75.51

GC content: 73.5

Gene sequence:

>936_bases
ATGAACGACGCCTCCGCTCCCCCGCCGGCTCCCACCACGGTCGCCCAGGCCCTCGCGCACGCACAGGCAATCGGCCTCGC
GCGCATCGATGCGCAACTGCTGCTGCTGCACACGCTGGGCCGGCCGGATGCAGGCCGTGCCTGGCTGCTCGCCCATGACG
AGGACCGGCTCTCCGCCGAGGAGCAGCAGGGTTTCGATGCCCTGTGCGCGCGCCGGCAGGCCGGCGAGCCCGTGGCCTAC
CTCACGGGTCGCAAGGAGTTCTACGGCCTGCCGCTGCAGGTGGATGCGCGCGTGCTGGATCCGCGCCCCGACACCGAAAC
GCTGGTGGACTGGGCGCTGGAGGTCCTGCGGCCCCTGCCCTTGCCGCGCGTGGCGGACCTGGGCACGGGCAGCGGCGCCA
TCGCCCTGGCCCTGCGGCACGGTCTGCCCGGCGCGCAGGTGGTGCTGGCCGTGGATGCCAGCGCGGACGCGCTCGCGGTC
GCGCGGGCCAACGCGCGGCGGCTGCACCTTTCCGTGGACTTCGTCCGCACGAACTGGCTGGACGGCATCTCCGGCCCCTT
CGACGCCATCGTGTCCAACCCCCCCTACATCGAAGAAGACGACCCGCACCTGGCGGCGCTGGTGCACGAGCCGCGGCAGG
CCCTGGCGAGCGGGCCGGACGGGCTGGACGACATCCGCACCATCGTGGTGCAGTCCTCCAGTCGCCTTGCCCCGGGCGGA
TGGCTGCTGCTGGAGCATGGCTGGAACCAGGCACAGGCCGTGCAGGCGCTGCTGCGCAACGCGGGCTATGCCGAGGTGCA
GAGCCGCGCCGACCTCGCCGGCCATGCGCGCTGCACCGGCGGACGCATGCCTGGCGCGCCTGCCGCGGCCGCACCCCTGG
AAACCCCGGCGCATGCCTCCACATGGTGCATGCCTCCCTCTTCGGGGCCGCAGTGA

Upstream 100 bases:

>100_bases
ATGGAAGGCGACCTGGGCGAAGTGCTGGAAGCCCTGCGCCACGCGCGCGAGGCCGAACTGCTGGCCGAGCTGGAATCGGC
GGCCTGAAGTCCACCGCGCC

Downstream 100 bases:

>100_bases
AATAATCCCCCCATGGCCGCCCGACCGCGGCCCCTTACAGGAGCAACCCATGAGCGACAACACCCAGCAACGCATCGACG
CCCTCGTCAAGTCCAACGAC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNDASAPPPAPTTVAQALAHAQAIGLARIDAQLLLLHTLGRPDAGRAWLLAHDEDRLSAEEQQGFDALCARRQAGEPVAY
LTGRKEFYGLPLQVDARVLDPRPDTETLVDWALEVLRPLPLPRVADLGTGSGAIALALRHGLPGAQVVLAVDASADALAV
ARANARRLHLSVDFVRTNWLDGISGPFDAIVSNPPYIEEDDPHLAALVHEPRQALASGPDGLDDIRTIVVQSSSRLAPGG
WLLLEHGWNQAQAVQALLRNAGYAEVQSRADLAGHARCTGGRMPGAPAAAAPLETPAHASTWCMPPSSGPQ

Sequences:

>Translated_311_residues
MNDASAPPPAPTTVAQALAHAQAIGLARIDAQLLLLHTLGRPDAGRAWLLAHDEDRLSAEEQQGFDALCARRQAGEPVAY
LTGRKEFYGLPLQVDARVLDPRPDTETLVDWALEVLRPLPLPRVADLGTGSGAIALALRHGLPGAQVVLAVDASADALAV
ARANARRLHLSVDFVRTNWLDGISGPFDAIVSNPPYIEEDDPHLAALVHEPRQALASGPDGLDDIRTIVVQSSSRLAPGG
WLLLEHGWNQAQAVQALLRNAGYAEVQSRADLAGHARCTGGRMPGAPAAAAPLETPAHASTWCMPPSSGPQ
>Mature_311_residues
MNDASAPPPAPTTVAQALAHAQAIGLARIDAQLLLLHTLGRPDAGRAWLLAHDEDRLSAEEQQGFDALCARRQAGEPVAY
LTGRKEFYGLPLQVDARVLDPRPDTETLVDWALEVLRPLPLPRVADLGTGSGAIALALRHGLPGAQVVLAVDASADALAV
ARANARRLHLSVDFVRTNWLDGISGPFDAIVSNPPYIEEDDPHLAALVHEPRQALASGPDGLDDIRTIVVQSSSRLAPGG
WLLLEHGWNQAQAVQALLRNAGYAEVQSRADLAGHARCTGGRMPGAPAAAAPLETPAHASTWCMPPSSGPQ

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=251, Percent_Identity=33.0677290836653, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1787463, Length=271, Percent_Identity=44.6494464944649, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=36.0406091370558, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24582226, Length=217, Percent_Identity=29.0322580645161, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32823; Mature: 32823

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDASAPPPAPTTVAQALAHAQAIGLARIDAQLLLLHTLGRPDAGRAWLLAHDEDRLSAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCH
EQQGFDALCARRQAGEPVAYLTGRKEFYGLPLQVDARVLDPRPDTETLVDWALEVLRPLP
HHCCHHHHHHHHHCCCCEEEEECCHHHCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCC
LPRVADLGTGSGAIALALRHGLPGAQVVLAVDASADALAVARANARRLHLSVDFVRTNWL
CCCHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEEEECCCC
DGISGPFDAIVSNPPYIEEDDPHLAALVHEPRQALASGPDGLDDIRTIVVQSSSRLAPGG
CCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
WLLLEHGWNQAQAVQALLRNAGYAEVQSRADLAGHARCTGGRMPGAPAAAAPLETPAHAS
EEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCC
TWCMPPSSGPQ
EEECCCCCCCC
>Mature Secondary Structure
MNDASAPPPAPTTVAQALAHAQAIGLARIDAQLLLLHTLGRPDAGRAWLLAHDEDRLSAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCH
EQQGFDALCARRQAGEPVAYLTGRKEFYGLPLQVDARVLDPRPDTETLVDWALEVLRPLP
HHCCHHHHHHHHHCCCCEEEEECCHHHCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCC
LPRVADLGTGSGAIALALRHGLPGAQVVLAVDASADALAVARANARRLHLSVDFVRTNWL
CCCHHCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEEEEECCCC
DGISGPFDAIVSNPPYIEEDDPHLAALVHEPRQALASGPDGLDDIRTIVVQSSSRLAPGG
CCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
WLLLEHGWNQAQAVQALLRNAGYAEVQSRADLAGHARCTGGRMPGAPAAAAPLETPAHAS
EEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCC
TWCMPPSSGPQ
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]