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The map label for this gene is prfA [H]

Identifier: 120612308

GI number: 120612308

Start: 4040862

End: 4041944

Strand: Direct

Name: prfA [H]

Synonym: Aave_3664

Alternate gene names: 120612308

Gene position: 4040862-4041944 (Clockwise)

Preceding gene: 120612307

Following gene: 120612309

Centisome position: 75.49

GC content: 69.62

Gene sequence:

>1083_bases
ATGAAACCCTTCCTCAGAAGCCAGCTGGAGCGCTATGCCCAGCGCCTCGAAGAGCTCGACTTCCTGCTCTCGCGCGAGGA
CATCATGTCCGACATGGCCCAGTACCGCACCATCTCGCGCGAGCACGCCGAGGTGACGCAGGTCGCGGGCCGCTATACAC
GCTACCGCCAGCGCGAGGCCGACCTCGCCGGCGCGCGCGAGATGCTGGACGACCCGGACATGGCCGACATGGCCCGGGAA
GAGATCGCCGCCGCCGAGGCCGAACTGGTGCAGCTGGAGGATGAACTGCAGCGCCTGCTGCTGCCCCGCGACCCCGACGA
GGCGCGCAATGCCTTCCTGGAAATCCGCGCCGGCACCGGTGGCGACGAATCCGCGCTGTTCGCGGGCGACCTGGCCCGCA
TGTACACGCGCTACGCGGCCACGGCCGGCTGGAAGGTGGAGATCCTGAGCGCCAGCGACAACGAGATCGGCGGCTACAAG
GAAGTGGTGCTGCGCGTGGAAGGCGACGGCGTCTATGGCGCGCTGCGCTTCGAATCCGGCGGCCACCGCGTGCAGCGCGT
GCCCGCCACCGAGACCCAGGGCCGCATCCATACCAGCGCCTGCACCGTGGCCGTGATGCCCGAGCCCGATGAGCAGCAGG
CGATCACGCTGAACCCGGCCGACCTGCGCATCGACACCTTCCGGGCCAGCGGCGCGGGCGGCCAGCACATCAACAAGACC
GACTCCGCCGTGCGCGTGGTCCACCTGCCCACCGGCATCGTGGCCGAGTGCCAGGACGGCCGCAGCCAGCACAGCAACAA
GGCCAAGGCGCTGCAGGTGCTGCAGGCGCGCATCCAGGAAAAGGAACGCAGCGAGCGTGCCGCCAAGGAGGCCGCGCTGC
GCAAGGGCCTCGTGGGTTCGGGCGACCGCTCCGACCGCATCCGCACCTACAACTTCCCGCAGGGCCGGCTCACCGACCAC
CGCATCAACCTCACGCTCTACAAGCTGCTCGCCATCATGGAAGGCGACCTGGGCGAAGTGCTGGAAGCCCTGCGCCACGC
GCGCGAGGCCGAACTGCTGGCCGAGCTGGAATCGGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCCAGGCCTCTTCTGCCCTCTCCGGCCTCCCGCATCCGCGCACGGATGCCCAGGCTGCGAACCACCGCCGCAGCCA
CCCTTCTCCCTCTCCCGACA

Downstream 100 bases:

>100_bases
AGTCCACCGCGCCATGAACGACGCCTCCGCTCCCCCGCCGGCTCCCACCACGGTCGCCCAGGCCCTCGCGCACGCACAGG
CAATCGGCCTCGCGCGCATC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPFLRSQLERYAQRLEELDFLLSREDIMSDMAQYRTISREHAEVTQVAGRYTRYRQREADLAGAREMLDDPDMADMARE
EIAAAEAELVQLEDELQRLLLPRDPDEARNAFLEIRAGTGGDESALFAGDLARMYTRYAATAGWKVEILSASDNEIGGYK
EVVLRVEGDGVYGALRFESGGHRVQRVPATETQGRIHTSACTVAVMPEPDEQQAITLNPADLRIDTFRASGAGGQHINKT
DSAVRVVHLPTGIVAECQDGRSQHSNKAKALQVLQARIQEKERSERAAKEAALRKGLVGSGDRSDRIRTYNFPQGRLTDH
RINLTLYKLLAIMEGDLGEVLEALRHAREAELLAELESAA

Sequences:

>Translated_360_residues
MKPFLRSQLERYAQRLEELDFLLSREDIMSDMAQYRTISREHAEVTQVAGRYTRYRQREADLAGAREMLDDPDMADMARE
EIAAAEAELVQLEDELQRLLLPRDPDEARNAFLEIRAGTGGDESALFAGDLARMYTRYAATAGWKVEILSASDNEIGGYK
EVVLRVEGDGVYGALRFESGGHRVQRVPATETQGRIHTSACTVAVMPEPDEQQAITLNPADLRIDTFRASGAGGQHINKT
DSAVRVVHLPTGIVAECQDGRSQHSNKAKALQVLQARIQEKERSERAAKEAALRKGLVGSGDRSDRIRTYNFPQGRLTDH
RINLTLYKLLAIMEGDLGEVLEALRHAREAELLAELESAA
>Mature_360_residues
MKPFLRSQLERYAQRLEELDFLLSREDIMSDMAQYRTISREHAEVTQVAGRYTRYRQREADLAGAREMLDDPDMADMARE
EIAAAEAELVQLEDELQRLLLPRDPDEARNAFLEIRAGTGGDESALFAGDLARMYTRYAATAGWKVEILSASDNEIGGYK
EVVLRVEGDGVYGALRFESGGHRVQRVPATETQGRIHTSACTVAVMPEPDEQQAITLNPADLRIDTFRASGAGGQHINKT
DSAVRVVHLPTGIVAECQDGRSQHSNKAKALQVLQARIQEKERSERAAKEAALRKGLVGSGDRSDRIRTYNFPQGRLTDH
RINLTLYKLLAIMEGDLGEVLEALRHAREAELLAELESAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=45.0980392156863, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=38.622754491018, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=45.1776649746193, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=60.1123595505618, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=37.3134328358209, Blast_Score=221, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=319, Percent_Identity=35.1097178683386, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=366, Percent_Identity=41.5300546448087, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19921226, Length=328, Percent_Identity=40.5487804878049, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40088; Mature: 40088

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFLRSQLERYAQRLEELDFLLSREDIMSDMAQYRTISREHAEVTQVAGRYTRYRQREA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAGAREMLDDPDMADMAREEIAAAEAELVQLEDELQRLLLPRDPDEARNAFLEIRAGTG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEECCCC
GDESALFAGDLARMYTRYAATAGWKVEILSASDNEIGGYKEVVLRVEGDGVYGALRFESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEPDEQQAITLNPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVVHLPTGIVAECQDGRSQHSNKAKALQVLQARIQEKERSERAAKEAALRKGLVGS
CCEEEEEECCCCCCHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRIRTYNFPQGRLTDHRINLTLYKLLAIMEGDLGEVLEALRHAREAELLAELESAA
CCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPFLRSQLERYAQRLEELDFLLSREDIMSDMAQYRTISREHAEVTQVAGRYTRYRQREA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAGAREMLDDPDMADMAREEIAAAEAELVQLEDELQRLLLPRDPDEARNAFLEIRAGTG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCEEEEEECCCC
GDESALFAGDLARMYTRYAATAGWKVEILSASDNEIGGYKEVVLRVEGDGVYGALRFESG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEPDEQQAITLNPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVVHLPTGIVAECQDGRSQHSNKAKALQVLQARIQEKERSERAAKEAALRKGLVGS
CCEEEEEECCCCCCHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRIRTYNFPQGRLTDHRINLTLYKLLAIMEGDLGEVLEALRHAREAELLAELESAA
CCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA