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The map label for this gene is odhL [H]

Identifier: 120611904

GI number: 120611904

Start: 3582831

End: 3584258

Strand: Reverse

Name: odhL [H]

Synonym: Aave_3246

Alternate gene names: 120611904

Gene position: 3584258-3582831 (Counterclockwise)

Preceding gene: 120611905

Following gene: 120611903

Centisome position: 66.96

GC content: 67.51

Gene sequence:

>1428_bases
ATGAGCAAACAATTCGACGTCATCGTCATCGGCGGCGGTCCCGGTGGCTACATCGCCGCCATCCGCGCCGCCCAGCTGGG
CTTCAACGTGGCCTGCATCGACGAGTGGAAGAACGAGAAGGGCGGCCCCGCGCCCGGCGGCACCTGCACGAACGTGGGCT
GCATCCCCTCCAAGGCGCTGCTGCAGTCCTCCGAGCATTTCGAGCATGCCAACAAGCATTTCGCCGAGCACGGCATCACG
GCCACCGATGTGAAGATCGACGTGGCCAAGATGATCGGCCGCAAGGACGCCGTCGTGAAGCAGAACAACGACGGCATCCT
CTACCTGTTCAAGAAGAACAAGGTCAGCTTCTTCCATGGCCGCGGCTCCTTCGTGAAGGCCGCGGAGGGCGGCTACGAGA
TCCAGGTGGCCGGCGCCGCGCAGGAAACCCTGGTGGGCAAGCAGATCATCGTGGCCACGGGCTCCAACCCCCGCGCGCTG
CCCGGCGTGCCCTTCGACGAAGAGAAGGTGCTCTCCAACGACGGCGCGCTGCGCATCGGCGCCACGCCGAAGAAGCTGGG
CCTGATCGGTGCCGGCGTGATCGGCCTGGAGATGGGCTCCGTGTGGCGCCGCGTGGGTGCCGACGTGACGGTGCTCGAAG
GCCTGCCGACGTTCCTGGGCGCTGTCGATGAGCAGATCGCCAAGGAAGCCAAGAAGGCCTTCGACAAGCAGGGCCTGAAG
ATCGAGCTGGGCGTGAAGATCGGCGAGATCAAGACCGGCGGCGAGGGCGTCACGGTGGCCTACACCGACGCCAAGGGCGA
AGCCCGCGAGCTGGCGGTGGACAAGCTCATCGTCTCCATCGGCCGCGTGCCCAACACCATCGGCCTGAATCCCGAGGCCG
TGGGCCTGCAGCTCGACGAGCGCGGCGCCATCGTGGTCGATGCCGACTGCAGGACCAACCTGCCCGGCGTGTGGGCGGTG
GGCGACGTGGTGCGCGGCCCGATGCTGGCGCACAAGGCCGAGGAAGAGGGCGTGGCGGTGGCCGAGCGCATCGCGGGCCA
GCACGGCCACGTGAACTTCAACACCATCCCCTGGGTCATCTACACCAGCCCCGAGATCGCCTGGGTGGGCCGCACCGAGC
AGCAGCTCAAGGCCGACGGCGTGCAGTACAAGGCTGGCACGTTCCCGTTCCTGGCGAACGGCCGTGCCCGCGCGCTGGGC
GACACGACCGGCATGGTCAAGTTCCTGGCCGATGCGGTCACGGACGAGATCCTGGGCGTGCACATCGTGGGCCCGATGGC
CAGCGAGCTGATCTCCGAGGCCGTGGTGGCCATGGAGTTCAAGGCCAGCGCCGAAGACATCGCGCGCATCTGCCACGCCC
ACCCCTCGCTGTCCGAGGCGACCAAGGAAGCGGCCCTGGCCGTGGACAAGCGCACGCTCAACTTCTGA

Upstream 100 bases:

>100_bases
AGCGCCGTGAAGACCGAAATCCATTGACGGACCCACATGCATGGTTGGCCCCGGCCAGCCGGGCAGTGGGTCTTTCTCCA
ACTGACACAGTGAGAACGAC

Downstream 100 bases:

>100_bases
TCGCGCGCCGATCGGGAGCGCGCCGCGCTCCCCCGGGCTGCCGGCGCGCGCATCCGCGTGCCGGCAGCCGTTGTTTTTCA
TGTGGATGCGTTGATCGATT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSEHFEHANKHFAEHGIT
ATDVKIDVAKMIGRKDAVVKQNNDGILYLFKKNKVSFFHGRGSFVKAAEGGYEIQVAGAAQETLVGKQIIVATGSNPRAL
PGVPFDEEKVLSNDGALRIGATPKKLGLIGAGVIGLEMGSVWRRVGADVTVLEGLPTFLGAVDEQIAKEAKKAFDKQGLK
IELGVKIGEIKTGGEGVTVAYTDAKGEARELAVDKLIVSIGRVPNTIGLNPEAVGLQLDERGAIVVDADCRTNLPGVWAV
GDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGRTEQQLKADGVQYKAGTFPFLANGRARALG
DTTGMVKFLADAVTDEILGVHIVGPMASELISEAVVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF

Sequences:

>Translated_475_residues
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSEHFEHANKHFAEHGIT
ATDVKIDVAKMIGRKDAVVKQNNDGILYLFKKNKVSFFHGRGSFVKAAEGGYEIQVAGAAQETLVGKQIIVATGSNPRAL
PGVPFDEEKVLSNDGALRIGATPKKLGLIGAGVIGLEMGSVWRRVGADVTVLEGLPTFLGAVDEQIAKEAKKAFDKQGLK
IELGVKIGEIKTGGEGVTVAYTDAKGEARELAVDKLIVSIGRVPNTIGLNPEAVGLQLDERGAIVVDADCRTNLPGVWAV
GDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGRTEQQLKADGVQYKAGTFPFLANGRARALG
DTTGMVKFLADAVTDEILGVHIVGPMASELISEAVVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF
>Mature_474_residues
SKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSEHFEHANKHFAEHGITA
TDVKIDVAKMIGRKDAVVKQNNDGILYLFKKNKVSFFHGRGSFVKAAEGGYEIQVAGAAQETLVGKQIIVATGSNPRALP
GVPFDEEKVLSNDGALRIGATPKKLGLIGAGVIGLEMGSVWRRVGADVTVLEGLPTFLGAVDEQIAKEAKKAFDKQGLKI
ELGVKIGEIKTGGEGVTVAYTDAKGEARELAVDKLIVSIGRVPNTIGLNPEAVGLQLDERGAIVVDADCRTNLPGVWAVG
DVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGRTEQQLKADGVQYKAGTFPFLANGRARALGD
TTGMVKFLADAVTDEILGVHIVGPMASELISEAVVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=47.1816283924843, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=27.2340425531915, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI22035672, Length=479, Percent_Identity=27.9749478079332, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519430, Length=444, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=444, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=444, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277071, Length=444, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277065, Length=444, Percent_Identity=25, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=451, Percent_Identity=24.6119733924612, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI291045268, Length=445, Percent_Identity=24.0449438202247, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786307, Length=460, Percent_Identity=37.8260869565217, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=30.7855626326964, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081717, Length=473, Percent_Identity=28.3298097251586, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=27.8969957081545, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=47.2222222222222, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=29.3032786885246, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=399, Percent_Identity=30.0751879699248, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=399, Percent_Identity=30.0751879699248, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=482, Percent_Identity=26.3485477178423, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17559934, Length=205, Percent_Identity=32.6829268292683, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=49.2693110647182, Blast_Score=422, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=31.1203319502075, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.2151898734177, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=49.0405117270789, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24640551, Length=493, Percent_Identity=27.3833671399594, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=27.3833671399594, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=27.3833671399594, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17737741, Length=491, Percent_Identity=26.8839103869654, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50111; Mature: 49980

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNEKGGPAPGGTCTNVGCIPSKAL
CCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCHHH
LQSSEHFEHANKHFAEHGITATDVKIDVAKMIGRKDAVVKQNNDGILYLFKKNKVSFFHG
HHCHHHHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCEEEECCCCEEEEEECCCEEEEEC
RGSFVKAAEGGYEIQVAGAAQETLVGKQIIVATGSNPRALPGVPFDEEKVLSNDGALRIG
CCCEEEECCCCEEEEECCCCHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEC
ATPKKLGLIGAGVIGLEMGSVWRRVGADVTVLEGLPTFLGAVDEQIAKEAKKAFDKQGLK
CCCHHHCEEECCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCE
IELGVKIGEIKTGGEGVTVAYTDAKGEARELAVDKLIVSIGRVPNTIGLNPEAVGLQLDE
EEEEEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
RGAIVVDADCRTNLPGVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI
CCCEEEECCCCCCCCCEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCEEE
YTSPEIAWVGRTEQQLKADGVQYKAGTFPFLANGRARALGDTTGMVKFLADAVTDEILGV
ECCCCEEEECCCHHHHHHCCEEEECCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHEE
HIVGPMASELISEAVVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF
EEECHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCHHHHHHHHHHEECHHCCCC
>Mature Secondary Structure 
SKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNEKGGPAPGGTCTNVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCHHH
LQSSEHFEHANKHFAEHGITATDVKIDVAKMIGRKDAVVKQNNDGILYLFKKNKVSFFHG
HHCHHHHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCEEEECCCCEEEEEECCCEEEEEC
RGSFVKAAEGGYEIQVAGAAQETLVGKQIIVATGSNPRALPGVPFDEEKVLSNDGALRIG
CCCEEEECCCCEEEEECCCCHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEC
ATPKKLGLIGAGVIGLEMGSVWRRVGADVTVLEGLPTFLGAVDEQIAKEAKKAFDKQGLK
CCCHHHCEEECCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCE
IELGVKIGEIKTGGEGVTVAYTDAKGEARELAVDKLIVSIGRVPNTIGLNPEAVGLQLDE
EEEEEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
RGAIVVDADCRTNLPGVWAVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVI
CCCEEEECCCCCCCCCEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCEECCCCCEEE
YTSPEIAWVGRTEQQLKADGVQYKAGTFPFLANGRARALGDTTGMVKFLADAVTDEILGV
ECCCCEEEECCCHHHHHHCCEEEECCCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHEE
HIVGPMASELISEAVVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF
EEECHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCHHHHHHHHHHEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]