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The map label for this gene is sucB [H]

Identifier: 120611905

GI number: 120611905

Start: 3584511

End: 3585794

Strand: Reverse

Name: sucB [H]

Synonym: Aave_3247

Alternate gene names: 120611905

Gene position: 3585794-3584511 (Counterclockwise)

Preceding gene: 120611906

Following gene: 120611904

Centisome position: 66.99

GC content: 68.46

Gene sequence:

>1284_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAACTGTCCGAATCCGTGGCCGAGGCCACGATGCTCTCCTGGAAGAAGAAGGC
CGGCGAGGCCGTCGCCATCGATGAGATCCTGATCGAGATCGAAACCGACAAGGTCGTGCTCGAAGTGCCCGCGCCGGCCG
CCGGCGTGCTGGCCGAGATCGTGCAGGGCGACGGTGCCACCGTGGTGGCCGACCAGGTCATCGCCAAGATCGACACCGAA
GGCAAGGCCGGTGCCGCCGCCGCGCCCGCGCAGTCCGCCCCGACCGCCGCCGCCCAGGCGCCCGCCGTGGCCGCCGCAGC
GGCCGACGCCGCCCCTGCCGCGGGCGGCTCCAAGGGTGACGTGGCCATGCCCGCCGCCGCCAAGCTGCTGGCCGACAACA
ACCTCTCCGTCTCGGCCGTGTCCGGCACCGGCAAGGACGGCCGCGTGACCAAGGGCGACGTGCTGGCTGCCGTGGCCGGC
GGTGCTGCCGCCAAGCCTGCCGCCGCCCCGGGCGCCATCCCCACGGGCGTGCCCACCAAGGCGCTGCCCCAGGTGGCATC
GCCCGCCGCGCCGAACCTGGGCGACCGCCCCGAGCAGCGCGTGCCCATGAGCCGCCTGCGCGCCCGCATCGCCGAGCGCC
TGCTGCAGTCGCAGTCCACCAACGCCATCCTGACCACCTTCAACGAGGTGAACATGGCCCCGGTGATGGAACTGCGCAAG
AAGTTCCAGGACAGCTTCACCAAGGAGCACGGCACCAAGCTGGGCTTCATGAGCTTCTTCGTGAAGGCGGCCGTGCACGC
GCTCAAGAAGTTCCCGGTGCTGAATGCCTCGGTGGACGGCAACGACATCGTCTATCACGGCTACTTCGACATCGGCATCG
CCGTCGGTTCGCCCCGTGGCCTGGTGGTGCCGATCCTGCGCAACGCCGACCAGATGAGCTTCGCCGACATCGAGAAGAAG
ATCGCCGAGTTCGGCAAGAAGGCCCAGGAAGGCAAGCTCGGCATCGAGGAGATGACCGGCGGCACGTTCTCGATCTCCAA
CGGCGGCACCTTCGGCTCGATGCTGTCCACCCCCATCATCAACCCGCCGCAATCGGCCATCCTGGGCGTGCACGCCACCA
AGGACCGCGCCGTGGTGGAAAACGGCCAGATCGTGGTGCGCCCGATGAACTACCTGGCGATGTCGTACGACCACCGCATC
ATCGACGGCCGCGAAGCCGTGCTGGGCCTGGTGGCGATGAAGGACGCGCTGGAAGATCCGGCCCGCCTGCTGTTCGATAT
TTGA

Upstream 100 bases:

>100_bases
TCAAGGGTTTCGTCCTCGCCAAGTGAGCGCGTGAATCCCGACCCCGCGAACCGCATCCCACAGCGACACAACACGTTCTG
AAAGAATCGAAAGAATCGTC

Downstream 100 bases:

>100_bases
TGGACCAACCCCCTGAGGCGCCTTGCGCCTTCCCCCTTCTCTCGGCTTGCTGCGCAATCCGGGAAGGGGGACACCGCCAG
CGCTGCGGGGCGGCCCTTGC

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAIVEVKVPQLSESVAEATMLSWKKKAGEAVAIDEILIEIETDKVVLEVPAPAAGVLAEIVQGDGATVVADQVIAKIDTE
GKAGAAAAPAQSAPTAAAQAPAVAAAAADAAPAAGGSKGDVAMPAAAKLLADNNLSVSAVSGTGKDGRVTKGDVLAAVAG
GAAAKPAAAPGAIPTGVPTKALPQVASPAAPNLGDRPEQRVPMSRLRARIAERLLQSQSTNAILTTFNEVNMAPVMELRK
KFQDSFTKEHGTKLGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKK
IAEFGKKAQEGKLGIEEMTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRI
IDGREAVLGLVAMKDALEDPARLLFDI

Sequences:

>Translated_427_residues
MAIVEVKVPQLSESVAEATMLSWKKKAGEAVAIDEILIEIETDKVVLEVPAPAAGVLAEIVQGDGATVVADQVIAKIDTE
GKAGAAAAPAQSAPTAAAQAPAVAAAAADAAPAAGGSKGDVAMPAAAKLLADNNLSVSAVSGTGKDGRVTKGDVLAAVAG
GAAAKPAAAPGAIPTGVPTKALPQVASPAAPNLGDRPEQRVPMSRLRARIAERLLQSQSTNAILTTFNEVNMAPVMELRK
KFQDSFTKEHGTKLGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKK
IAEFGKKAQEGKLGIEEMTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRI
IDGREAVLGLVAMKDALEDPARLLFDI
>Mature_426_residues
AIVEVKVPQLSESVAEATMLSWKKKAGEAVAIDEILIEIETDKVVLEVPAPAAGVLAEIVQGDGATVVADQVIAKIDTEG
KAGAAAAPAQSAPTAAAQAPAVAAAAADAAPAAGGSKGDVAMPAAAKLLADNNLSVSAVSGTGKDGRVTKGDVLAAVAGG
AAAKPAAAPGAIPTGVPTKALPQVASPAAPNLGDRPEQRVPMSRLRARIAERLLQSQSTNAILTTFNEVNMAPVMELRKK
FQDSFTKEHGTKLGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKI
AEFGKKAQEGKLGIEEMTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRII
DGREAVLGLVAMKDALEDPARLLFDI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=252, Percent_Identity=54.7619047619048, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI31711992, Length=454, Percent_Identity=30.6167400881057, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=30.9050772626932, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=30.6345733041575, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI110671329, Length=442, Percent_Identity=28.0542986425339, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.6459627329193, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=50.8196721311475, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=32.5635103926097, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=43.7352245862884, Blast_Score=330, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17560088, Length=457, Percent_Identity=31.0722100656455, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=27.9445727482679, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=334, Percent_Identity=32.3353293413174, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=41.0926365795724, Blast_Score=309, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=29.1666666666667, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6321632, Length=182, Percent_Identity=29.1208791208791, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=56.3318777292576, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI18859875, Length=445, Percent_Identity=26.9662921348315, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=30.8695652173913, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=31.140350877193, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44052; Mature: 43921

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVAEATMLSWKKKAGEAVAIDEILIEIETDKVVLEVPAPAAGVLAEI
CEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCHHHHHHHH
VQGDGATVVADQVIAKIDTEGKAGAAAAPAQSAPTAAAQAPAVAAAAADAAPAAGGSKGD
HCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCC
VAMPAAAKLLADNNLSVSAVSGTGKDGRVTKGDVLAAVAGGAAAKPAAAPGAIPTGVPTK
EECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHH
ALPQVASPAAPNLGDRPEQRVPMSRLRARIAERLLQSQSTNAILTTFNEVNMAPVMELRK
HHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
KFQDSFTKEHGTKLGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCC
LVVPILRNADQMSFADIEKKIAEFGKKAQEGKLGIEEMTGGTFSISNGGTFGSMLSTPII
EEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHCCCEEEECCCCCHHHHHCCCCC
NPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGREAVLGLVAMKDALEDP
CCCHHHEEEEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCH
ARLLFDI
HHHHHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVAEATMLSWKKKAGEAVAIDEILIEIETDKVVLEVPAPAAGVLAEI
EEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCEEEEECCCCHHHHHHHH
VQGDGATVVADQVIAKIDTEGKAGAAAAPAQSAPTAAAQAPAVAAAAADAAPAAGGSKGD
HCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHCCCCCCCCCCCCC
VAMPAAAKLLADNNLSVSAVSGTGKDGRVTKGDVLAAVAGGAAAKPAAAPGAIPTGVPTK
EECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHH
ALPQVASPAAPNLGDRPEQRVPMSRLRARIAERLLQSQSTNAILTTFNEVNMAPVMELRK
HHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
KFQDSFTKEHGTKLGFMSFFVKAAVHALKKFPVLNASVDGNDIVYHGYFDIGIAVGSPRG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCC
LVVPILRNADQMSFADIEKKIAEFGKKAQEGKLGIEEMTGGTFSISNGGTFGSMLSTPII
EEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHCCCEEEECCCCCHHHHHCCCCC
NPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRIIDGREAVLGLVAMKDALEDP
CCCHHHEEEEECCCCCEEEECCCEEEEEHHHHHHHCCCEEECCHHHHHHHHHHHHHHCCH
ARLLFDI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]