Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 120611134

GI number: 120611134

Start: 2689314

End: 2691017

Strand: Direct

Name: pdhB [H]

Synonym: Aave_2463

Alternate gene names: 120611134

Gene position: 2689314-2691017 (Clockwise)

Preceding gene: 120611133

Following gene: 120611135

Centisome position: 50.24

GC content: 67.9

Gene sequence:

>1704_bases
ATGGCACTGATTGACATCAAGGTCCCGGACATCGGGGATTTCGACGCCGTCGGCGTGATCGAACTGCTGGTGAAGCCCGG
CGACACGGTGAAGGCCGAGCAGTCGCTCATCACCGTGGAGTCCGACAAGGCTTCCATGGAGATTCCTTCCAGCCACGCCG
GCGTGGTGAAGGAGGTCAAGGTCAAGATCGGCGACCAGGTGAGCGAAGGCTCGGTGATCGTCGTGGTCGAGGCCGAAGGC
GAGGGCGCGCAGCCCGCGGCGGCGCCTGCCGCGAAAGCGGAAGCGCCCGCACCCGCATCGGCCGCGGCGTCCGCGCCTGC
ACCTGCCGCGCCCGCGGCCGCCCCGGCTGCCGCCGGCCCGATCGATGTGAAGGTGCCGGACATCGGCGACTTCAAGGACG
TGGCGGTGATCGAACTGCTGGTGAAGGTCGGCGATACGGTCAAGGAAGAGCAGTCGCTCTTCACCGTCGAATCGGACAAG
GCCTCCATGGAGATTCCGTCGCCCGCCGCCGGCGTGGTGAAGGAGCTGAAGATCAAGATCGGCGACAAGGTGAACGTGGG
TGACCTCGTGGCCGTGCTCGAAGGCACCGCGGGCGCGTCCTCGGCGGCGCCTGCCGCTGCCGCTCCGGTGGACCGCGCCG
CGCCGCAGTCCGAGCCCGACGCGCGCGCCCCGCGCCAGCTGCCGGCCGAGGAGGCCGCCCCCCAGGCGGGAGCGCAGGCA
TCCTCGCCGTCTCCCGCGCCCGCGCCGCATGCCCCGGGCGCTGCGCCCATCGGCTTGCCGCATGCATCGCCTTCGGTGCG
CAAGTTCGCGCGTGAACTGGGCGTGCCGCTGGAAGAGCTCAAGGGCTCCGGCCCCAAGGGTCGCATCACCCAGGAGGACG
TGCAGGCCTTCACCAGGCAGGTGATGGCGGGCGGCGTGCAGACCAAGGCCCAGGCGGCCAAGCCACCTGCCGGCGGCGGC
TCCGGCGTGGGCATGGACCTGCTGCCCTGGCCGAAGGTGGACTTCGCCAAGTTCGGTGGCGTGGAGCGCAAGGATCTCTC
GCGCATCAAGAAGATCAGCGGCGCCAACCTGCACCGCAACTGGGTGATGATTCCCCACGTCACCAACAACGACGAAGCCG
ACATCACCGAACTGGAAGCCTTCCGCGTCTCCACCAACAAGGAGAACGAGAAGTCTGGCGTGAAGGTGACGATGCTCGCC
TTCGTGATCAAGGCCGTGGTGGCCGCGCTCAAGAAGTTCCCCGAGTTCAACACCAGCCTGGACGGCGACACGCTCGTCTA
CAAGCAGTACTTCCACATCGGTTTCGCGGCGGACACGCCGAACGGCCTCGTGGTGCCCGTGCTGAAGGATGCCGACAAGA
AGGGCATCCTGCAGATCAGCCAGGAAATGGGCGAGCTCGCCAAGAAGGCCCGCGACGGAAAGCTCGGCGCGGCCGACATG
CAGGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGTATCGGCGGCACGCACTTCACGCCCATCATCAACGCGCCCGAAGT
GGCCATCCTGGGCCTCTCGAAGGGCCAGATGAAGCCCGTGTGGGACGGCAAGCAGTTCGTGCCGCGCCTGGTGCTGCCGC
TGTCGCTCTCCTACGATCACCGCGTGATCGACGGAGCGTCTGCCGCGCGCTTCAACGCCTACCTGGGCCAGGTGCTGGCG
GATTACCGCCGCATCCTGCTGTGA

Upstream 100 bases:

>100_bases
AAGCTGCCCGCTGCCAAGGTGGCCGAGGCCATCCAGAAGTACGGCATCCAGGCCGACAAGGTGAACCCGCTGCACGCCTG
ACCCCACCGGAGACAACAAC

Downstream 100 bases:

>100_bases
AAGGAAACCCGACATGGCAGTGATCGACATCAAGGTGCCCGACATCGGTGATTTTTCCGAAGTGGGCGTGATCGAAGTGC
TGGTGAAAGCCGGCGACACC

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MALIDIKVPDIGDFDAVGVIELLVKPGDTVKAEQSLITVESDKASMEIPSSHAGVVKEVKVKIGDQVSEGSVIVVVEAEG
EGAQPAAAPAAKAEAPAPASAAASAPAPAAPAAAPAAAGPIDVKVPDIGDFKDVAVIELLVKVGDTVKEEQSLFTVESDK
ASMEIPSPAAGVVKELKIKIGDKVNVGDLVAVLEGTAGASSAAPAAAAPVDRAAPQSEPDARAPRQLPAEEAAPQAGAQA
SSPSPAPAPHAPGAAPIGLPHASPSVRKFARELGVPLEELKGSGPKGRITQEDVQAFTRQVMAGGVQTKAQAAKPPAGGG
SGVGMDLLPWPKVDFAKFGGVERKDLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLA
FVIKAVVAALKKFPEFNTSLDGDTLVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADM
QGGCFSISSLGGIGGTHFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDHRVIDGASAARFNAYLGQVLA
DYRRILL

Sequences:

>Translated_567_residues
MALIDIKVPDIGDFDAVGVIELLVKPGDTVKAEQSLITVESDKASMEIPSSHAGVVKEVKVKIGDQVSEGSVIVVVEAEG
EGAQPAAAPAAKAEAPAPASAAASAPAPAAPAAAPAAAGPIDVKVPDIGDFKDVAVIELLVKVGDTVKEEQSLFTVESDK
ASMEIPSPAAGVVKELKIKIGDKVNVGDLVAVLEGTAGASSAAPAAAAPVDRAAPQSEPDARAPRQLPAEEAAPQAGAQA
SSPSPAPAPHAPGAAPIGLPHASPSVRKFARELGVPLEELKGSGPKGRITQEDVQAFTRQVMAGGVQTKAQAAKPPAGGG
SGVGMDLLPWPKVDFAKFGGVERKDLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLA
FVIKAVVAALKKFPEFNTSLDGDTLVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADM
QGGCFSISSLGGIGGTHFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDHRVIDGASAARFNAYLGQVLA
DYRRILL
>Mature_566_residues
ALIDIKVPDIGDFDAVGVIELLVKPGDTVKAEQSLITVESDKASMEIPSSHAGVVKEVKVKIGDQVSEGSVIVVVEAEGE
GAQPAAAPAAKAEAPAPASAAASAPAPAAPAAAPAAAGPIDVKVPDIGDFKDVAVIELLVKVGDTVKEEQSLFTVESDKA
SMEIPSPAAGVVKELKIKIGDKVNVGDLVAVLEGTAGASSAAPAAAAPVDRAAPQSEPDARAPRQLPAEEAAPQAGAQAS
SPSPAPAPHAPGAAPIGLPHASPSVRKFARELGVPLEELKGSGPKGRITQEDVQAFTRQVMAGGVQTKAQAAKPPAGGGS
GVGMDLLPWPKVDFAKFGGVERKDLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAF
VIKAVVAALKKFPEFNTSLDGDTLVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADMQ
GGCFSISSLGGIGGTHFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDHRVIDGASAARFNAYLGQVLAD
YRRILL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=497, Percent_Identity=28.169014084507, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI31711992, Length=430, Percent_Identity=31.3953488372093, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=26.1538461538462, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=26.1538461538462, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=33.7662337662338, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786305, Length=567, Percent_Identity=52.3809523809524, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1786946, Length=451, Percent_Identity=29.490022172949, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=446, Percent_Identity=30.2690582959641, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=437, Percent_Identity=31.1212814645309, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=454, Percent_Identity=29.7356828193833, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=29.126213592233, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=431, Percent_Identity=31.0904872389791, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=27.1111111111111, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI18859875, Length=453, Percent_Identity=31.7880794701987, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24582497, Length=226, Percent_Identity=34.5132743362832, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129315, Length=203, Percent_Identity=37.4384236453202, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.4883720930233, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58655; Mature: 58523

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIDIKVPDIGDFDAVGVIELLVKPGDTVKAEQSLITVESDKASMEIPSSHAGVVKEVK
CEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHH
VKIGDQVSEGSVIVVVEAEGEGAQPAAAPAAKAEAPAPASAAASAPAPAAPAAAPAAAGP
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IDVKVPDIGDFKDVAVIELLVKVGDTVKEEQSLFTVESDKASMEIPSPAAGVVKELKIKI
CEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHC
GDKVNVGDLVAVLEGTAGASSAAPAAAAPVDRAAPQSEPDARAPRQLPAEEAAPQAGAQA
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCC
SSPSPAPAPHAPGAAPIGLPHASPSVRKFARELGVPLEELKGSGPKGRITQEDVQAFTRQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHH
VMAGGVQTKAQAAKPPAGGGSGVGMDLLPWPKVDFAKFGGVERKDLSRIKKISGANLHRN
HHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEECC
WVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAVVAALKKFPEFNTSL
EEEEEEECCCCCCCHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DGDTLVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADM
CCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QGGCFSISSLGGIGGTHFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDH
CCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCC
RVIDGASAARFNAYLGQVLADYRRILL
EEECCCCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ALIDIKVPDIGDFDAVGVIELLVKPGDTVKAEQSLITVESDKASMEIPSSHAGVVKEVK
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHH
VKIGDQVSEGSVIVVVEAEGEGAQPAAAPAAKAEAPAPASAAASAPAPAAPAAAPAAAGP
HHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IDVKVPDIGDFKDVAVIELLVKVGDTVKEEQSLFTVESDKASMEIPSPAAGVVKELKIKI
CEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHC
GDKVNVGDLVAVLEGTAGASSAAPAAAAPVDRAAPQSEPDARAPRQLPAEEAAPQAGAQA
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCC
SSPSPAPAPHAPGAAPIGLPHASPSVRKFARELGVPLEELKGSGPKGRITQEDVQAFTRQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHH
VMAGGVQTKAQAAKPPAGGGSGVGMDLLPWPKVDFAKFGGVERKDLSRIKKISGANLHRN
HHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCEECC
WVMIPHVTNNDEADITELEAFRVSTNKENEKSGVKVTMLAFVIKAVVAALKKFPEFNTSL
EEEEEEECCCCCCCHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DGDTLVYKQYFHIGFAADTPNGLVVPVLKDADKKGILQISQEMGELAKKARDGKLGAADM
CCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QGGCFSISSLGGIGGTHFTPIINAPEVAILGLSKGQMKPVWDGKQFVPRLVLPLSLSYDH
CCCEEEHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEECCCCC
RVIDGASAARFNAYLGQVLADYRRILL
EEECCCCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]