Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 120611135

GI number: 120611135

Start: 2691031

End: 2692884

Strand: Direct

Name: lpdA [H]

Synonym: Aave_2464

Alternate gene names: 120611135

Gene position: 2691031-2692884 (Clockwise)

Preceding gene: 120611134

Following gene: 120611137

Centisome position: 50.27

GC content: 68.28

Gene sequence:

>1854_bases
ATGGCAGTGATCGACATCAAGGTGCCCGACATCGGTGATTTTTCCGAAGTGGGCGTGATCGAAGTGCTGGTGAAAGCCGG
CGACACCATCAAGGTGGAACAGAGCCTCATCACCGTCGAATCCGACAAGGCATCGATGGAGATTCCGTCGAGCCATGCCG
GCGTCGTCAAGGAGGTGAAGGTGAAGGTCGGCGACAAGGTCGCCGAAGGTTCGGTGGTGCTGACGCTGGAGGCCGCGGAT
GCGGCTGCCGCTGCGGCACCGGCGCCCGCATCCGCACCCGCGGCGGCTGCAGCGCCTGCCCAGGCTGCTGCACCGGCCCC
CAAGGCCGCAGCCGCGCCGGCTCCGGCCGCGGCGAGCTTCGGCGGCAATGCCGACGTCGAATGCGACGTGCTCGTGCTGG
GCGGCGGCCCCGGCGGCTACAGCGCCGCCTTCCGCGCGGCCGACCTGGGCCTGAAGGTCGTCATCGTGGAGCGCTATTCC
ACCCTGGGCGGCGTCTGCCTGAACGTGGGCTGCATCCCGTCCAAGGCGCTGCTGCACGTGGCGGCGGTGATCGACGAGGT
GAGCCACCTCAAGGCGGCCGGCATCGAGTTCGGTGCACCGAAGGTGGACATCGACACCCTGCGCGGCCACAAGGAAAAGG
TCATCGGCAAGCTGACCGGCGGTCTGGCCGCCATGGCCAAGATGCGCAAGGTGACCACCGTGCGCGGCGTGGGTCAGTTC
GTGGGCGCCAACCACCTGGAGGTGGAGGAAACCACCGGCGCCAGGGGCCAGGAGAAGACGGGCGGCAAGAAGGTCGTGGC
CTTCAAGCGCGCGATCATCGCCGCCGGCTCCCAGGCCGTGCACCTGCCGTTCATGCCGAATGATCCGCGCGTGGTCGATT
CGACCGGCGCACTGGAGTTGAAGGAGGTGCCGAAGCGCATGCTGATCCTGGGCGGCGGCATCATCGGCCTGGAAATGGGC
ACCGTCTATTCCACCCTGGGCGCGCGCCTGGACGTGGTCGAGATGATGGACGGCCTGATGCAGGGCGCCGACCGCGACCT
GGTCAAGGTGTGGCAGAAGATGAATGCCCACCGCTTCGACAACATCATGCTCAAGACCAAGACGGTGGGGGCCGAGGCCA
CGCCCGAGGGCATCAAGGTCACGTTCGCCGCGGCGGAAGAGGGTGGCAAGGCGCCCGAGCCGCAGGTCTATGACCTGGTG
CTGCAGGCCGTGGGCCGCACCCCCAACGGCAAGAAGATCGGCGCCGAGAAGGCCGGCGTGGCCGTGACCGACCGCGGCTT
CATCGACGTGGACGTGCAGATGCGCACCAACGTGCCGCACATCTTCGCCATCGGCGACATCGTGGGCCAGCCCATGCTGG
CGCACAAGGCGGTGCACGAAGCGCACGTGGCGGCCGAGGTCATCGCCGGTGAACTGACGGGCGACAAGGCGCTGGCCTCC
GCTGCGTTCAACGCCCGCGTGATCCCGAGCGTGGCCTACACCGACCCCGAAGTGGCCTGGGTGGGCCTGACCGAGGACCA
GGCCAAGGCCCAGGGCATCAAGGTCCGGAAGGGCCTGTTCCCCTGGACGGCCTCCGGCCGCGCCATCGCCAACGGCCGCG
ACGAGGGCTATACGAAGCTGCTGTTCGACGATTCGCCCGAAGCCCATGGCCACGGCAGGATCCTGGGCGGCGGCATCGTG
GGCACCCATGCCGGCGACATGATCGGCGAGATCGCCCTGGCGATCGAGATGGGCGCGGATGCCGTGGACATCGGCAAGAC
CATCCATCCGCACCCGACGCTGGGCGAGAGCATCGGCATGGCCGCGGAGATCGCGCACGGCTCCTGCACGGACGTGCCGC
CGCAGAAGAAGTGA

Upstream 100 bases:

>100_bases
CACCGCGTGATCGACGGAGCGTCTGCCGCGCGCTTCAACGCCTACCTGGGCCAGGTGCTGGCGGATTACCGCCGCATCCT
GCTGTGAAAGGAAACCCGAC

Downstream 100 bases:

>100_bases
AAGACGGGTGGCGCAGTGAATCGCTGCGCCGCTTTAATGGGCACTTGCCGAGTCCGTGCGCCACCTGAAAGCCGGGCTGC
GCCTGACCAGCGCGGAACTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MAVIDIKVPDIGDFSEVGVIEVLVKAGDTIKVEQSLITVESDKASMEIPSSHAGVVKEVKVKVGDKVAEGSVVLTLEAAD
AAAAAAPAPASAPAAAAAPAQAAAPAPKAAAAPAPAAASFGGNADVECDVLVLGGGPGGYSAAFRAADLGLKVVIVERYS
TLGGVCLNVGCIPSKALLHVAAVIDEVSHLKAAGIEFGAPKVDIDTLRGHKEKVIGKLTGGLAAMAKMRKVTTVRGVGQF
VGANHLEVEETTGARGQEKTGGKKVVAFKRAIIAAGSQAVHLPFMPNDPRVVDSTGALELKEVPKRMLILGGGIIGLEMG
TVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNAHRFDNIMLKTKTVGAEATPEGIKVTFAAAEEGGKAPEPQVYDLV
LQAVGRTPNGKKIGAEKAGVAVTDRGFIDVDVQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELTGDKALAS
AAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVRKGLFPWTASGRAIANGRDEGYTKLLFDDSPEAHGHGRILGGGIV
GTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQKK

Sequences:

>Translated_617_residues
MAVIDIKVPDIGDFSEVGVIEVLVKAGDTIKVEQSLITVESDKASMEIPSSHAGVVKEVKVKVGDKVAEGSVVLTLEAAD
AAAAAAPAPASAPAAAAAPAQAAAPAPKAAAAPAPAAASFGGNADVECDVLVLGGGPGGYSAAFRAADLGLKVVIVERYS
TLGGVCLNVGCIPSKALLHVAAVIDEVSHLKAAGIEFGAPKVDIDTLRGHKEKVIGKLTGGLAAMAKMRKVTTVRGVGQF
VGANHLEVEETTGARGQEKTGGKKVVAFKRAIIAAGSQAVHLPFMPNDPRVVDSTGALELKEVPKRMLILGGGIIGLEMG
TVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNAHRFDNIMLKTKTVGAEATPEGIKVTFAAAEEGGKAPEPQVYDLV
LQAVGRTPNGKKIGAEKAGVAVTDRGFIDVDVQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELTGDKALAS
AAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVRKGLFPWTASGRAIANGRDEGYTKLLFDDSPEAHGHGRILGGGIV
GTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQKK
>Mature_616_residues
AVIDIKVPDIGDFSEVGVIEVLVKAGDTIKVEQSLITVESDKASMEIPSSHAGVVKEVKVKVGDKVAEGSVVLTLEAADA
AAAAAPAPASAPAAAAAPAQAAAPAPKAAAAPAPAAASFGGNADVECDVLVLGGGPGGYSAAFRAADLGLKVVIVERYST
LGGVCLNVGCIPSKALLHVAAVIDEVSHLKAAGIEFGAPKVDIDTLRGHKEKVIGKLTGGLAAMAKMRKVTTVRGVGQFV
GANHLEVEETTGARGQEKTGGKKVVAFKRAIIAAGSQAVHLPFMPNDPRVVDSTGALELKEVPKRMLILGGGIIGLEMGT
VYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNAHRFDNIMLKTKTVGAEATPEGIKVTFAAAEEGGKAPEPQVYDLVL
QAVGRTPNGKKIGAEKAGVAVTDRGFIDVDVQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELTGDKALASA
AFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVRKGLFPWTASGRAIANGRDEGYTKLLFDDSPEAHGHGRILGGGIVG
THAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGMAAEIAHGSCTDVPPQKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=39.6659707724426, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI50301238, Length=486, Percent_Identity=27.7777777777778, Blast_Score=146, Evalue=7e-35,
Organism=Homo sapiens, GI291045266, Length=498, Percent_Identity=27.9116465863454, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI33519430, Length=474, Percent_Identity=27.2151898734177, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519428, Length=474, Percent_Identity=27.2151898734177, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI33519426, Length=474, Percent_Identity=27.2151898734177, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=27.2151898734177, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=27.2151898734177, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI291045268, Length=491, Percent_Identity=26.2729124236253, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI22035672, Length=493, Percent_Identity=28.3975659229209, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1786307, Length=496, Percent_Identity=61.491935483871, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI87082354, Length=486, Percent_Identity=26.3374485596708, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789915, Length=464, Percent_Identity=29.0948275862069, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI87081717, Length=485, Percent_Identity=25.7731958762887, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=61.6438356164384, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=475, Percent_Identity=38.3157894736842, Blast_Score=306, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17557007, Length=521, Percent_Identity=26.4875239923225, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=485, Percent_Identity=24.5360824742268, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71983419, Length=485, Percent_Identity=24.5360824742268, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=469, Percent_Identity=25.3731343283582, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=39.5010395010395, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6325240, Length=492, Percent_Identity=28.6585365853659, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=493, Percent_Identity=25.5578093306288, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI21358499, Length=477, Percent_Identity=39.832285115304, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24640551, Length=542, Percent_Identity=28.7822878228782, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=509, Percent_Identity=28.2907662082515, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640549, Length=509, Percent_Identity=28.2907662082515, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=25.6048387096774, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 63398; Mature: 63266

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIDIKVPDIGDFSEVGVIEVLVKAGDTIKVEQSLITVESDKASMEIPSSHAGVVKEVK
CEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCEECCCCCCCHHEEEE
VKVGDKVAEGSVVLTLEAADAAAAAAPAPASAPAAAAAPAQAAAPAPKAAAAPAPAAASF
EECCCEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHCC
GGNADVECDVLVLGGGPGGYSAAFRAADLGLKVVIVERYSTLGGVCLNVGCIPSKALLHV
CCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHH
AAVIDEVSHLKAAGIEFGAPKVDIDTLRGHKEKVIGKLTGGLAAMAKMRKVTTVRGVGQF
HHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGANHLEVEETTGARGQEKTGGKKVVAFKRAIIAAGSQAVHLPFMPNDPRVVDSTGALEL
HCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCEEECCCCCEEH
KEVPKRMLILGGGIIGLEMGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNAHRFD
HHCCCEEEEECCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCC
NIMLKTKTVGAEATPEGIKVTFAAAEEGGKAPEPQVYDLVLQAVGRTPNGKKIGAEKAGV
CEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
AVTDRGFIDVDVQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELTGDKALAS
EEECCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVRKGLFPWTASGRAIANGRDEGYTKL
HHCCCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCEEE
LFDDSPEAHGHGRILGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGM
EECCCCCCCCCCEEEECCEECCCCHHHHHHEEEEEECCCCHHHCCCCCCCCCCCCHHCCC
AAEIAHGSCTDVPPQKK
EEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AVIDIKVPDIGDFSEVGVIEVLVKAGDTIKVEQSLITVESDKASMEIPSSHAGVVKEVK
EEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEEEEEECCCCCEECCCCCCCHHEEEE
VKVGDKVAEGSVVLTLEAADAAAAAAPAPASAPAAAAAPAQAAAPAPKAAAAPAPAAASF
EECCCEECCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCHHCC
GGNADVECDVLVLGGGPGGYSAAFRAADLGLKVVIVERYSTLGGVCLNVGCIPSKALLHV
CCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHH
AAVIDEVSHLKAAGIEFGAPKVDIDTLRGHKEKVIGKLTGGLAAMAKMRKVTTVRGVGQF
HHHHHHHHHHHHCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGANHLEVEETTGARGQEKTGGKKVVAFKRAIIAAGSQAVHLPFMPNDPRVVDSTGALEL
HCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCEEECCCCCEEH
KEVPKRMLILGGGIIGLEMGTVYSTLGARLDVVEMMDGLMQGADRDLVKVWQKMNAHRFD
HHCCCEEEEECCCEEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCC
NIMLKTKTVGAEATPEGIKVTFAAAEEGGKAPEPQVYDLVLQAVGRTPNGKKIGAEKAGV
CEEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCE
AVTDRGFIDVDVQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEVIAGELTGDKALAS
EEECCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AAFNARVIPSVAYTDPEVAWVGLTEDQAKAQGIKVRKGLFPWTASGRAIANGRDEGYTKL
HHCCCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCEEE
LFDDSPEAHGHGRILGGGIVGTHAGDMIGEIALAIEMGADAVDIGKTIHPHPTLGESIGM
EECCCCCCCCCCEEEECCEECCCCHHHHHHEEEEEECCCCHHHCCCCCCCCCCCCHHCCC
AAEIAHGSCTDVPPQKK
EEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]