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The map label for this gene is gshA [H]

Identifier: 120610936

GI number: 120610936

Start: 2492143

End: 2493699

Strand: Direct

Name: gshA [H]

Synonym: Aave_2262

Alternate gene names: 120610936

Gene position: 2492143-2493699 (Clockwise)

Preceding gene: 120610934

Following gene: 120610938

Centisome position: 46.56

GC content: 71.16

Gene sequence:

>1557_bases
ATGACGAGCCATCTGCAGGAGAGAATCCGCGCGCTGCCAGGCAACCGGCTGGCGGGCATGCGCCGCGGCATCGAGAAGGA
GGGCCTGCGCGTGCTGGCCGAGGGCGGGCTGGCGCTCACGCCCCATCCGGCGGCGCTGGGCTCCGCGCTCACGCACCCGC
ACATCACGACCGACTACAGCGAGTCGCAGATCGAACTCATCACCGGCGCGCGTGGCTCGGTGCGGGAATGCCTCGACGAG
CTGACCGAGATCCACCAGTTCGTGCTGCGCACCCTGGCGCAGTCCGGGGAGATGCTGTGGGTATCGAGCATGCCCTGCGG
ACTGCCCACGGACGAGACCATCCCCCTGGCGCGCTTCGGCTCCTCCAACGTGGGGCGCGCCAAGAGCATCTACCGCATGG
GCCTGGGGCACCGCTACGGCCGCCGCATGCAGACCATCTCGGGCATCCACTACAACTGGTCCATGCCCGGCATCGCGAGC
GACGAGTATTTCGGGCTGATCCGCAACTTCCGGCGCCAGGCCTTCCTGCTGCTCTACCTCTTCGGCGCATCGCCCGCGCT
GTGCGACTGCTTCGTGCAGGGCCGCCAGCACGGCCTGCAGCGCCTGGGGCCGGATTCCCTCTACCTGCCGCACGCCACCT
CGCTGCGCATGGGCCGCCTGGGCTACCAGAGCGATGCCCAGGCCACGCTGGCCTTGAGCTACAACGGGCTCGACGGCTAT
GCCGACTCCCTGCACGAAGCACTGACGCGGCCCTATCCCGCCTACGAGGCGCTGGGCGTGCGCAACCCCGGCGGCGACTA
CAACCAGCTGGGCACCAGCCTGCTGCAGATCGAGAACGAGTTCTACGGCACCATCCGCCCCAAGCGCACCGTGCGCCCCG
GCGAACGCCCCCTGCACGCGCTGCGCGACCGCGGCGTGGAATACGTGGAGGTGCGGCTCATGGACCTCGATCCTTTCGCC
CCCGTGGGCATCGAGGCGCCGACCATGCGGCTGCTGGACATGTTCCTGCTGCACTGCCTGCTGTCGGACAGCCCGCCCGA
CACCCCCGCCGAGATCGCCACCCTCAAGCGCAACCAGCACCTCACGGCCGAGCGCGGCCGCGAGCCCGGGCTGTGCCTGG
AGCGTGCCGGCCGCAGCGTGCCGCTGGTGGAGTGGGGCGCCGAGATCCTGGCCGAATGCGCGCCCATCGCCGCCGCGCTG
GACGCGGCCCAGGGCACGGATGCGCATGCGCAGGTCCTGCAGGCGGCAAGGAACCTGCTGCGCGAGCCCGATGCCACGCC
GTCCGCCCGCGTGCTCGCGCGCATGGCCGGCGGCCACGGCCAGAGCTTCCTGGGCTTCACGCTCGCGCAGTCCCGTGCTT
CCCGCGACACCCTGCTGCAGCAGCCCTGGGCCGGTGAGCAGCAGGCACGCTACGAGCGCGTGGCCGCCGGGTCGCTGGCA
GAGCAGAAGGCCATCGAGGCCGCCGACACGCTGGACTTCGAGAGCTGGCGCAAGCGCTACATGGCCATCGGCGCGGCCTC
GTCCCTGGCGGACGCGCCGCTCGCGCAGACCGTATAG

Upstream 100 bases:

>100_bases
CGGCGCGAAGCAGGGACTTGCCCGCGGGCCCGAGCGCGAGCGTTGCCGGCGTGCTAAGTTCCGTCCGACACGGACCAACC
GGTAACAAAAAAGACGTACC

Downstream 100 bases:

>100_bases
GCCTGTTGCATAGGCTTTCTGTCAGCGGCCGGCCGCCTGCGCCGCGCCGCGGATGCCCGCCAGCGTACCCGTGGGCGTGA
TCGCCTCCGCGTCCAGCAGG

Product: glutamate-cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTSHLQERIRALPGNRLAGMRRGIEKEGLRVLAEGGLALTPHPAALGSALTHPHITTDYSESQIELITGARGSVRECLDE
LTEIHQFVLRTLAQSGEMLWVSSMPCGLPTDETIPLARFGSSNVGRAKSIYRMGLGHRYGRRMQTISGIHYNWSMPGIAS
DEYFGLIRNFRRQAFLLLYLFGASPALCDCFVQGRQHGLQRLGPDSLYLPHATSLRMGRLGYQSDAQATLALSYNGLDGY
ADSLHEALTRPYPAYEALGVRNPGGDYNQLGTSLLQIENEFYGTIRPKRTVRPGERPLHALRDRGVEYVEVRLMDLDPFA
PVGIEAPTMRLLDMFLLHCLLSDSPPDTPAEIATLKRNQHLTAERGREPGLCLERAGRSVPLVEWGAEILAECAPIAAAL
DAAQGTDAHAQVLQAARNLLREPDATPSARVLARMAGGHGQSFLGFTLAQSRASRDTLLQQPWAGEQQARYERVAAGSLA
EQKAIEAADTLDFESWRKRYMAIGAASSLADAPLAQTV

Sequences:

>Translated_518_residues
MTSHLQERIRALPGNRLAGMRRGIEKEGLRVLAEGGLALTPHPAALGSALTHPHITTDYSESQIELITGARGSVRECLDE
LTEIHQFVLRTLAQSGEMLWVSSMPCGLPTDETIPLARFGSSNVGRAKSIYRMGLGHRYGRRMQTISGIHYNWSMPGIAS
DEYFGLIRNFRRQAFLLLYLFGASPALCDCFVQGRQHGLQRLGPDSLYLPHATSLRMGRLGYQSDAQATLALSYNGLDGY
ADSLHEALTRPYPAYEALGVRNPGGDYNQLGTSLLQIENEFYGTIRPKRTVRPGERPLHALRDRGVEYVEVRLMDLDPFA
PVGIEAPTMRLLDMFLLHCLLSDSPPDTPAEIATLKRNQHLTAERGREPGLCLERAGRSVPLVEWGAEILAECAPIAAAL
DAAQGTDAHAQVLQAARNLLREPDATPSARVLARMAGGHGQSFLGFTLAQSRASRDTLLQQPWAGEQQARYERVAAGSLA
EQKAIEAADTLDFESWRKRYMAIGAASSLADAPLAQTV
>Mature_517_residues
TSHLQERIRALPGNRLAGMRRGIEKEGLRVLAEGGLALTPHPAALGSALTHPHITTDYSESQIELITGARGSVRECLDEL
TEIHQFVLRTLAQSGEMLWVSSMPCGLPTDETIPLARFGSSNVGRAKSIYRMGLGHRYGRRMQTISGIHYNWSMPGIASD
EYFGLIRNFRRQAFLLLYLFGASPALCDCFVQGRQHGLQRLGPDSLYLPHATSLRMGRLGYQSDAQATLALSYNGLDGYA
DSLHEALTRPYPAYEALGVRNPGGDYNQLGTSLLQIENEFYGTIRPKRTVRPGERPLHALRDRGVEYVEVRLMDLDPFAP
VGIEAPTMRLLDMFLLHCLLSDSPPDTPAEIATLKRNQHLTAERGREPGLCLERAGRSVPLVEWGAEILAECAPIAAALD
AAQGTDAHAQVLQAARNLLREPDATPSARVLARMAGGHGQSFLGFTLAQSRASRDTLLQQPWAGEQQARYERVAAGSLAE
QKAIEAADTLDFESWRKRYMAIGAASSLADAPLAQTV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=509, Percent_Identity=37.9174852652259, Blast_Score=344, Evalue=6e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 56744; Mature: 56613

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHLQERIRALPGNRLAGMRRGIEKEGLRVLAEGGLALTPHPAALGSALTHPHITTDYS
CCHHHHHHHHHCCCCHHHHHHCCCHHHCCEEEECCCEEECCCHHHHHHHHCCCCCCCCCC
ESQIELITGARGSVRECLDELTEIHQFVLRTLAQSGEMLWVSSMPCGLPTDETIPLARFG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCC
SSNVGRAKSIYRMGLGHRYGRRMQTISGIHYNWSMPGIASDEYFGLIRNFRRQAFLLLYL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FGASPALCDCFVQGRQHGLQRLGPDSLYLPHATSLRMGRLGYQSDAQATLALSYNGLDGY
HCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHCCCCCCCCCCEEEEEEECCCCHH
ADSLHEALTRPYPAYEALGVRNPGGDYNQLGTSLLQIENEFYGTIRPKRTVRPGERPLHA
HHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LRDRGVEYVEVRLMDLDPFAPVGIEAPTMRLLDMFLLHCLLSDSPPDTPAEIATLKRNQH
HHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
LTAERGREPGLCLERAGRSVPLVEWGAEILAECAPIAAALDAAQGTDAHAQVLQAARNLL
CCHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
REPDATPSARVLARMAGGHGQSFLGFTLAQSRASRDTLLQQPWAGEQQARYERVAAGSLA
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EQKAIEAADTLDFESWRKRYMAIGAASSLADAPLAQTV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
TSHLQERIRALPGNRLAGMRRGIEKEGLRVLAEGGLALTPHPAALGSALTHPHITTDYS
CHHHHHHHHHCCCCHHHHHHCCCHHHCCEEEECCCEEECCCHHHHHHHHCCCCCCCCCC
ESQIELITGARGSVRECLDELTEIHQFVLRTLAQSGEMLWVSSMPCGLPTDETIPLARFG
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCC
SSNVGRAKSIYRMGLGHRYGRRMQTISGIHYNWSMPGIASDEYFGLIRNFRRQAFLLLYL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FGASPALCDCFVQGRQHGLQRLGPDSLYLPHATSLRMGRLGYQSDAQATLALSYNGLDGY
HCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHCCCCCCCCCCEEEEEEECCCCHH
ADSLHEALTRPYPAYEALGVRNPGGDYNQLGTSLLQIENEFYGTIRPKRTVRPGERPLHA
HHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LRDRGVEYVEVRLMDLDPFAPVGIEAPTMRLLDMFLLHCLLSDSPPDTPAEIATLKRNQH
HHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
LTAERGREPGLCLERAGRSVPLVEWGAEILAECAPIAAALDAAQGTDAHAQVLQAARNLL
CCHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
REPDATPSARVLARMAGGHGQSFLGFTLAQSRASRDTLLQQPWAGEQQARYERVAAGSLA
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EQKAIEAADTLDFESWRKRYMAIGAASSLADAPLAQTV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA