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The map label for this gene is rpfG [H]

Identifier: 120610742

GI number: 120610742

Start: 2264020

End: 2265207

Strand: Direct

Name: rpfG [H]

Synonym: Aave_2064

Alternate gene names: 120610742

Gene position: 2264020-2265207 (Clockwise)

Preceding gene: 120610741

Following gene: 120610752

Centisome position: 42.3

GC content: 66.75

Gene sequence:

>1188_bases
GTGAGGCGGCCCGTCCCTCCGCGCCGCAGGCCATCCGAAAGACACAGGCACAGCAACACCATGGACACTTCGCTGCTCTC
CGATGAACCCATGCTCAAGGACCGGACCATCGCCACGGTGCTGGTGGTGGACGACACCCCCGACAACCTCGCGCTCATGG
CCACGCTGCTGCGCGACCATTACCTCGTCAAGGTGGCCAGCCACGGCGAGAAGGCGCTGCGCATCGCCCAGTCGGACCCG
CCGCCCGACCTGATCCTGCTGGACATCATGATGCCCGGCCTGGACGGCTACGAGGTCTGCCGGCAGCTCAAGGCGCACCC
CGCCTCGCGCGACATTCCCATCATTTTCCTGACCGCCCGCAGCACGCCGGAGGACGAGCGGCGCGGCCTGGCCGCCGGGG
CGGTGGACTACATCACCAAGCCCATCAGTCCGCCGATCCTGCTGGCGCGGGTGCACACCCACCTGACGCTGAAGGCCACG
GCGGATTTCCTGCGCGACAAGAGCGCGTACCTGGAGCGCGAGGTGGCCCTGCGGACGCTGGAGGTGCAGGCCATCCAGGA
CGTGACCATCATGGCCATGACCTCGCTCGCGGAAACGCGCGACAACGAGACCGGCAACCACATCCGGCGCACGCAGCTCT
ACGTCAAGGCCCTGGCCGAGCGCCTCGCACGCCATCCGCGCTTCGAGGCGCTGCTCAACGAGCGCATGATCGATCTGCTC
TACAAATCGGCGCCGCTGCACGACATCGGCAAGATCGGCATTCCCGACAGCATCCTCCTCAAGCCGGGCAAGCTCACCGC
CGAGGAGTTCGAGGTCATGAAGACCCACACCACGCTCGGCCGCGACGCCATCGAGAACGCCGAGCGGCGGCTCGGCATGC
GGGTGGCGTTCCTGAGCGTGTCCAAGGAGATCGCCTACAGCCACCAGGAGAAATGGGACGGCACCGGCTACCCGCTGGGG
CTCGCGGGCGACGCGATTCCCGTTTCTGCGCGGCTGATGGCGGTGGCCGACGTGTACGACGCGCTCATCAGCAAGCGCGT
GTACAAGCCCGCCTTCTCGCACGAGCAGGCCTGCACCACGATCCTGAAGGGCCGGGGATCGCATTTCGATCCCGACATGG
TGGATGCCTTCATGGAACTGGCCGAGGATTTCCGCCAGATCGCGTTGAAATACCCGGACCCGGAGTGA

Upstream 100 bases:

>100_bases
GCTTCGCCGCCGTGGAGACGCACGTGCGCGACTTCGATTTCGAACACGCGCTGGCGATCATCGACGCGCCGCCGGAGCCC
TCCACCGTTTCCGGCGCCGC

Downstream 100 bases:

>100_bases
TGGCGCGCGGCCGCCGCGTCAGCCGGCGCGCCCGTGCAGGCCCGCATCGGCACCGGCACCGCCGGTTTCCCGGAATGCCT
CGTACCAGGCGCGGCCCGCG

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRRPVPPRRRPSERHRHSNTMDTSLLSDEPMLKDRTIATVLVVDDTPDNLALMATLLRDHYLVKVASHGEKALRIAQSDP
PPDLILLDIMMPGLDGYEVCRQLKAHPASRDIPIIFLTARSTPEDERRGLAAGAVDYITKPISPPILLARVHTHLTLKAT
ADFLRDKSAYLEREVALRTLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLARHPRFEALLNERMIDLL
YKSAPLHDIGKIGIPDSILLKPGKLTAEEFEVMKTHTTLGRDAIENAERRLGMRVAFLSVSKEIAYSHQEKWDGTGYPLG
LAGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTTILKGRGSHFDPDMVDAFMELAEDFRQIALKYPDPE

Sequences:

>Translated_395_residues
MRRPVPPRRRPSERHRHSNTMDTSLLSDEPMLKDRTIATVLVVDDTPDNLALMATLLRDHYLVKVASHGEKALRIAQSDP
PPDLILLDIMMPGLDGYEVCRQLKAHPASRDIPIIFLTARSTPEDERRGLAAGAVDYITKPISPPILLARVHTHLTLKAT
ADFLRDKSAYLEREVALRTLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLARHPRFEALLNERMIDLL
YKSAPLHDIGKIGIPDSILLKPGKLTAEEFEVMKTHTTLGRDAIENAERRLGMRVAFLSVSKEIAYSHQEKWDGTGYPLG
LAGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTTILKGRGSHFDPDMVDAFMELAEDFRQIALKYPDPE
>Mature_395_residues
MRRPVPPRRRPSERHRHSNTMDTSLLSDEPMLKDRTIATVLVVDDTPDNLALMATLLRDHYLVKVASHGEKALRIAQSDP
PPDLILLDIMMPGLDGYEVCRQLKAHPASRDIPIIFLTARSTPEDERRGLAAGAVDYITKPISPPILLARVHTHLTLKAT
ADFLRDKSAYLEREVALRTLEVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLARHPRFEALLNERMIDLL
YKSAPLHDIGKIGIPDSILLKPGKLTAEEFEVMKTHTTLGRDAIENAERRLGMRVAFLSVSKEIAYSHQEKWDGTGYPLG
LAGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTTILKGRGSHFDPDMVDAFMELAEDFRQIALKYPDPE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=41.0714285714286, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788394, Length=123, Percent_Identity=41.4634146341463, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=42.1487603305785, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082012, Length=95, Percent_Identity=42.1052631578947, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=39.0476190476191, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786784, Length=95, Percent_Identity=40, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=36.8852459016393, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 44333; Mature: 44333

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPVPPRRRPSERHRHSNTMDTSLLSDEPMLKDRTIATVLVVDDTPDNLALMATLLRDH
CCCCCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
YLVKVASHGEKALRIAQSDPPPDLILLDIMMPGLDGYEVCRQLKAHPASRDIPIIFLTAR
HEEEEECCCCHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECC
STPEDERRGLAAGAVDYITKPISPPILLARVHTHLTLKATADFLRDKSAYLEREVALRTL
CCCCHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
EVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLARHPRFEALLNERMIDLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YKSAPLHDIGKIGIPDSILLKPGKLTAEEFEVMKTHTTLGRDAIENAERRLGMRVAFLSV
HHCCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKEIAYSHQEKWDGTGYPLGLAGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ILKGRGSHFDPDMVDAFMELAEDFRQIALKYPDPE
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRRPVPPRRRPSERHRHSNTMDTSLLSDEPMLKDRTIATVLVVDDTPDNLALMATLLRDH
CCCCCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
YLVKVASHGEKALRIAQSDPPPDLILLDIMMPGLDGYEVCRQLKAHPASRDIPIIFLTAR
HEEEEECCCCHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEECC
STPEDERRGLAAGAVDYITKPISPPILLARVHTHLTLKATADFLRDKSAYLEREVALRTL
CCCCHHHCCCHHHHHHHHHCCCCCCEEEEEHHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
EVQAIQDVTIMAMTSLAETRDNETGNHIRRTQLYVKALAERLARHPRFEALLNERMIDLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YKSAPLHDIGKIGIPDSILLKPGKLTAEEFEVMKTHTTLGRDAIENAERRLGMRVAFLSV
HHCCCCHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKEIAYSHQEKWDGTGYPLGLAGDAIPVSARLMAVADVYDALISKRVYKPAFSHEQACTT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ILKGRGSHFDPDMVDAFMELAEDFRQIALKYPDPE
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]