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The map label for this gene is otsA [H]

Identifier: 120610752

GI number: 120610752

Start: 2275280

End: 2276926

Strand: Direct

Name: otsA [H]

Synonym: Aave_2074

Alternate gene names: 120610752

Gene position: 2275280-2276926 (Clockwise)

Preceding gene: 120610742

Following gene: 120610753

Centisome position: 42.51

GC content: 70.31

Gene sequence:

>1647_bases
ATGGTCCGACAGGTATCCGGAGCGCGCCGCAGCGCGTCCCCCCCAGCATTGCAGGCACAGGGCGAGGCCCGCCAGACGTC
CTCCGCCCGTCCGCCGTCAGCCGGCAGGGCACTGGAGCGCACCGCGGTCCAGCCCCGCCGCAAGAGGCCGTTCGATGGTT
CCGGCGCGGCCCAGCCGCAAGCCCGGCAGCGGCAGCGCCCTCCGCAGAACCGGCTGGTCGTCGTCTCCAACCGGATGGTG
GACCCGTCGAAGCCCGCTGCCGGCGGCCTGGCCGTCGCTCTGGCCGAAACCATGCACGACAACCCGGGGCTCTGGATGGG
CTGGAGCGGCGCCATCGGCGGGCGGCCTGGCACGCCCAGCGTGCGAACGCAGGCTTTCGGCCAGTCCACCCTGGTGGGAA
TGGACCTGAGTCGTACCCAGTTCCGGCAGTACTACGCGGGTTACAGCAATTCGGTGCTCTGGCCCGTGATGCACAGCAGT
GCCGGCTGGGCCGACATCCGGCCGGGCCAGTACGGCGTCTATCGCCAAGTGAACCGGATATTCGCCGCCCAGCTCGCCCC
GCTGCTGCAGCAGGACGACATGCTGTGGATCCACGACTACCACCTCATTCCCCTCGCGCAGGAGCTGCGCCGGCTCGGGT
GCCGGCAGCGCATGGGCTTCTTCAACCACATCCCGTTCCCGGAACCGGGGGTCTTCCGGGCCATTCCGGAGCACAAGGAA
CTGGTGGAGTCGCTTTTCTCCTATGACCTGGTGGGCATGCAGATACCGCGGGACGTGGAGAACTTCCGGGACTATGTGCG
CGAGGAGCGCATCGGGGAAGCCGCTGCGGACGCAAGCCTGCTGCGCGCTTTCGGGCGCCAGGTGCAGGTGCGGGATTTCC
CGATCGGCATCGATGTCCAGGGCCTGATGGCGCAGGAGCCCGACACCGGCCCCAGCGGAGTCCTGGAGCGCATGCGCACC
GAGAAGGCCGAAGGGCGGCTCCTCATGGTGGGGGTGGAGCGGCTGGATTACTCCAAGGGCGTGCCCGATCGGTTCGCCGC
GCTCGGGCAGTTGCTGGAAGACCGCCCGGATCTCCGGGAGAAGGTGACCTTCGTGCAGATCGGTGCGCCGTCCCGTCCGC
AGGTGCCGGCGTATGCCGCGCTGGCGCGCAGGACGCAGGAGATGGTGGACGGCATCAACAGCAAGTACGGGACGCCGTCG
TGGACGCCCATCCTGTACTTCGCCGAGCCGGTCCGGCGGAGCGCGCTGCCGGAGATCTACCGCATGGGCCGGGTGGGCGT
GGTGACTTCGGTGGCCGACGGCATGAACCTGGTGGCCAAGGAGTACGTCGCCTCGCAGGATGAGCGCGATCCCGGTGCGC
TGGTCCTCTCCCGGGGCGCCGGCGCGGCCCACCAGCTCCAGGACGCGATCCTGGTGCCGCCAGCGGACCGGCCCGCCATC
GCCCGGGCCTACGAGGCGGCGCTCTCCATGCCGCTGCAGGAGCGCAAGGAGCGCCATGCCCGGCTGCTGGACAACGTGAA
GACGCAGGATCTGGCGAAGTGGCGCGAGGATTACCTGTCCGCCCTGCGCGCCGTGCCGCAATCCGCACGCCAGCACGACG
GCGGGGTGTCCGGACGGAAGGCCGGGCGGCCGGCCGGCCCGCACTGA

Upstream 100 bases:

>100_bases
GCCCGTGCTCTCCATGGCCATCCGTGTTCCATATCCGGAAGCCGCGGGGGAGGCTGCATTTTCAATCAAGGGGCATTATC
CAGAAATTAAGGAGTCCACC

Downstream 100 bases:

>100_bases
GGGCACGGCAGGCCGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGGCGGGAGCGGCGCAGCGGCGACAAT
CGCGCGTCCTTTCCGCTGTG

Product: Alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MVRQVSGARRSASPPALQAQGEARQTSSARPPSAGRALERTAVQPRRKRPFDGSGAAQPQARQRQRPPQNRLVVVSNRMV
DPSKPAAGGLAVALAETMHDNPGLWMGWSGAIGGRPGTPSVRTQAFGQSTLVGMDLSRTQFRQYYAGYSNSVLWPVMHSS
AGWADIRPGQYGVYRQVNRIFAAQLAPLLQQDDMLWIHDYHLIPLAQELRRLGCRQRMGFFNHIPFPEPGVFRAIPEHKE
LVESLFSYDLVGMQIPRDVENFRDYVREERIGEAAADASLLRAFGRQVQVRDFPIGIDVQGLMAQEPDTGPSGVLERMRT
EKAEGRLLMVGVERLDYSKGVPDRFAALGQLLEDRPDLREKVTFVQIGAPSRPQVPAYAALARRTQEMVDGINSKYGTPS
WTPILYFAEPVRRSALPEIYRMGRVGVVTSVADGMNLVAKEYVASQDERDPGALVLSRGAGAAHQLQDAILVPPADRPAI
ARAYEAALSMPLQERKERHARLLDNVKTQDLAKWREDYLSALRAVPQSARQHDGGVSGRKAGRPAGPH

Sequences:

>Translated_548_residues
MVRQVSGARRSASPPALQAQGEARQTSSARPPSAGRALERTAVQPRRKRPFDGSGAAQPQARQRQRPPQNRLVVVSNRMV
DPSKPAAGGLAVALAETMHDNPGLWMGWSGAIGGRPGTPSVRTQAFGQSTLVGMDLSRTQFRQYYAGYSNSVLWPVMHSS
AGWADIRPGQYGVYRQVNRIFAAQLAPLLQQDDMLWIHDYHLIPLAQELRRLGCRQRMGFFNHIPFPEPGVFRAIPEHKE
LVESLFSYDLVGMQIPRDVENFRDYVREERIGEAAADASLLRAFGRQVQVRDFPIGIDVQGLMAQEPDTGPSGVLERMRT
EKAEGRLLMVGVERLDYSKGVPDRFAALGQLLEDRPDLREKVTFVQIGAPSRPQVPAYAALARRTQEMVDGINSKYGTPS
WTPILYFAEPVRRSALPEIYRMGRVGVVTSVADGMNLVAKEYVASQDERDPGALVLSRGAGAAHQLQDAILVPPADRPAI
ARAYEAALSMPLQERKERHARLLDNVKTQDLAKWREDYLSALRAVPQSARQHDGGVSGRKAGRPAGPH
>Mature_548_residues
MVRQVSGARRSASPPALQAQGEARQTSSARPPSAGRALERTAVQPRRKRPFDGSGAAQPQARQRQRPPQNRLVVVSNRMV
DPSKPAAGGLAVALAETMHDNPGLWMGWSGAIGGRPGTPSVRTQAFGQSTLVGMDLSRTQFRQYYAGYSNSVLWPVMHSS
AGWADIRPGQYGVYRQVNRIFAAQLAPLLQQDDMLWIHDYHLIPLAQELRRLGCRQRMGFFNHIPFPEPGVFRAIPEHKE
LVESLFSYDLVGMQIPRDVENFRDYVREERIGEAAADASLLRAFGRQVQVRDFPIGIDVQGLMAQEPDTGPSGVLERMRT
EKAEGRLLMVGVERLDYSKGVPDRFAALGQLLEDRPDLREKVTFVQIGAPSRPQVPAYAALARRTQEMVDGINSKYGTPS
WTPILYFAEPVRRSALPEIYRMGRVGVVTSVADGMNLVAKEYVASQDERDPGALVLSRGAGAAHQLQDAILVPPADRPAI
ARAYEAALSMPLQERKERHARLLDNVKTQDLAKWREDYLSALRAVPQSARQHDGGVSGRKAGRPAGPH

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=466, Percent_Identity=41.4163090128755, Blast_Score=325, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI115533558, Length=420, Percent_Identity=27.6190476190476, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI115533560, Length=420, Percent_Identity=27.6190476190476, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=414, Percent_Identity=27.7777777777778, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6319602, Length=468, Percent_Identity=30.982905982906, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6323537, Length=384, Percent_Identity=28.90625, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6320279, Length=401, Percent_Identity=24.9376558603491, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323917, Length=446, Percent_Identity=24.8878923766816, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19920676, Length=490, Percent_Identity=33.6734693877551, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 60548; Mature: 60548

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00276 CHANNEL_COLICIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRQVSGARRSASPPALQAQGEARQTSSARPPSAGRALERTAVQPRRKRPFDGSGAAQPQ
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCH
ARQRQRPPQNRLVVVSNRMVDPSKPAAGGLAVALAETMHDNPGLWMGWSGAIGGRPGTPS
HHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
VRTQAFGQSTLVGMDLSRTQFRQYYAGYSNSVLWPVMHSSAGWADIRPGQYGVYRQVNRI
HHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
FAAQLAPLLQQDDMLWIHDYHLIPLAQELRRLGCRQRMGFFNHIPFPEPGVFRAIPEHKE
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHH
LVESLFSYDLVGMQIPRDVENFRDYVREERIGEAAADASLLRAFGRQVQVRDFPIGIDVQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCH
GLMAQEPDTGPSGVLERMRTEKAEGRLLMVGVERLDYSKGVPDRFAALGQLLEDRPDLRE
HHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCHHH
KVTFVQIGAPSRPQVPAYAALARRTQEMVDGINSKYGTPSWTPILYFAEPVRRSALPEIY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHH
RMGRVGVVTSVADGMNLVAKEYVASQDERDPGALVLSRGAGAAHQLQDAILVPPADRPAI
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCEECCCCCCHHH
ARAYEAALSMPLQERKERHARLLDNVKTQDLAKWREDYLSALRAVPQSARQHDGGVSGRK
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGRPAGPH
CCCCCCCC
>Mature Secondary Structure
MVRQVSGARRSASPPALQAQGEARQTSSARPPSAGRALERTAVQPRRKRPFDGSGAAQPQ
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCHHCCCCCCCCCCCCH
ARQRQRPPQNRLVVVSNRMVDPSKPAAGGLAVALAETMHDNPGLWMGWSGAIGGRPGTPS
HHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
VRTQAFGQSTLVGMDLSRTQFRQYYAGYSNSVLWPVMHSSAGWADIRPGQYGVYRQVNRI
HHHHHCCCCEEEECCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHH
FAAQLAPLLQQDDMLWIHDYHLIPLAQELRRLGCRQRMGFFNHIPFPEPGVFRAIPEHKE
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHH
LVESLFSYDLVGMQIPRDVENFRDYVREERIGEAAADASLLRAFGRQVQVRDFPIGIDVQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCH
GLMAQEPDTGPSGVLERMRTEKAEGRLLMVGVERLDYSKGVPDRFAALGQLLEDRPDLRE
HHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCHHH
KVTFVQIGAPSRPQVPAYAALARRTQEMVDGINSKYGTPSWTPILYFAEPVRRSALPEIY
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHH
RMGRVGVVTSVADGMNLVAKEYVASQDERDPGALVLSRGAGAAHQLQDAILVPPADRPAI
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHCCCEECCCCCCHHH
ARAYEAALSMPLQERKERHARLLDNVKTQDLAKWREDYLSALRAVPQSARQHDGGVSGRK
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGRPAGPH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA