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The map label for this gene is pleD [H]

Identifier: 120610099

GI number: 120610099

Start: 1551950

End: 1553500

Strand: Reverse

Name: pleD [H]

Synonym: Aave_1412

Alternate gene names: 120610099

Gene position: 1553500-1551950 (Counterclockwise)

Preceding gene: 120610100

Following gene: 120610098

Centisome position: 29.02

GC content: 69.63

Gene sequence:

>1551_bases
ATGGCCGACCGCCCCCCTTCAGAGATCGCGCGAGAAACCCTCAAACAGCTGGCGGCCCGCCGCCTCGTGCCCACGCCGGA
CAACTACCAGGCGCTGTACGAGGAGATCGCCGGCACGCGCACGGCCCCGACCTTCCCCGATGCGCAACTGCGCCACATCC
TGCGGGTGCTCCCCGGCCAGACGCCCGCCCAGAAGCGGCTGCTCGGCCAGTTCGAGACGGCCATCGAACAGGCGGACTGG
TCGATGCTGCAGGGCGTGCTGGTGGGTTATGCGAACCTCGGTCTGCATGCCGGTGGCGCCACCCCCGTGGAGACGGCCCC
GGCACCGCTGACGGGCGGACCGACGGGCACCGCCACGGTGCTGCCCGAAGACATGGCGGAGCAACTGGCCCGTCTGGTCG
ACAACACGCTGCCGGCGCTGGGCGAGGACGACGCGCGCGTCCATGAACTGGCGGCGCAGCTCACGCATTTCCTGCGCCAG
CCCGGACCGCCGGTTTCCACGCTGGTGCTGATGCTCAACAACTTCAGCTACCGCCTCTCCTTCGCGACAGAGGACCAGGC
GGCGATCCGCGCCGGCCTGCTGACCCTGCTGCACATGGTGTTCGAGAACATCTCTGTACTGAGCAAGGACGACCACTGGC
TGCAGGGGCAGGCCGAGGCCCTCAAGGCGGCCTCCACGCCGCCGCTCACGCTGCGGCGGCTGGACGACGTGCAGCGCCGG
CTCAAGGACGTGATCTTCAAGCAGAACGAGGCACGCGAGCGCTCCGTGCAGGCGCAGCAGCAGATGAAGGAACTGCTGGC
CACGTTCATCGAGCGGCTCGCGCAGATCACCGCGTCCAGCAGCACGTACCACGAGAAGATGGAGCGCTGCGCCGAGCAGA
TCGGGCAGGCCACGAGCCTGGACCAGATCACGCCGCTGCTCGAGGAAGTGATGGGAGCGACCCGTGCCATGGCGCTCGAC
ACGCGCCTGACGCGCGACGAGCTGCAGGAGCTGCGCGAGCGCTCCGAGGCCCGGCACGCGGAGATCGCCAAGCTGCAGGA
GGAGCTGGACCGGGCCAGCGCCCAGGCGCGGCACGATCCGCTGACCGGCTCGCTGAACCGCAAGGGCCTGGACGAGGCGC
TGGAGCGCGAAGTGGCCCGTGCGCTGCGCAACGACGCGCCGCTGTGCGTCGCGCTGCTGGACCTGGACAATTTCAAGGCC
ATCAACGACCGGCTGGGGCATACGGGCGGGGACGAGGCGCTCAAGCACCTGGCAGGCGTGACGCGCGAGGTGATGCGTCC
GCAGGACCAGCTCGCCCGCTACGGGGGCGAGGAGTTCGTGATCATCCTGCCGGACACCGCGCTGGCCCAGGGCGTGGAGG
CCATGGCCCGCCTGCAGCGCGAGCTGACCACGCGCTACTTCCTCAAGGACCAGGAAAAGGTGCTCATCACCTTCAGCGCC
GGCGTGGCGCAGGTCGCGCCGCAGGAGACGACGGTGGAAGCGATCCGGCGGGCGGACCAGGGGATGTACCTCGCCAAGCG
CTCGGGCAAGAACCGTGTCATGGCGGCCTGA

Upstream 100 bases:

>100_bases
GGAACTGCCGCGCCGGCCCCGCGGGGCCGCTCCGGCCGCGCTGTTCAAGGTGTCCGCCGTCTGTCACACTCGAGATCCCA
TCCTCTGCCGTGCATGCCCG

Downstream 100 bases:

>100_bases
CCCGGCCCGGCACGCCCCGGACCGCCGCACGCTGCTGCGGCTCGCCGCCGCGCTGGCGGTGCCCGGCGCCCTGCCCCGCT
GGGCATGGTCCTCACCCGGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MADRPPSEIARETLKQLAARRLVPTPDNYQALYEEIAGTRTAPTFPDAQLRHILRVLPGQTPAQKRLLGQFETAIEQADW
SMLQGVLVGYANLGLHAGGATPVETAPAPLTGGPTGTATVLPEDMAEQLARLVDNTLPALGEDDARVHELAAQLTHFLRQ
PGPPVSTLVLMLNNFSYRLSFATEDQAAIRAGLLTLLHMVFENISVLSKDDHWLQGQAEALKAASTPPLTLRRLDDVQRR
LKDVIFKQNEARERSVQAQQQMKELLATFIERLAQITASSSTYHEKMERCAEQIGQATSLDQITPLLEEVMGATRAMALD
TRLTRDELQELRERSEARHAEIAKLQEELDRASAQARHDPLTGSLNRKGLDEALEREVARALRNDAPLCVALLDLDNFKA
INDRLGHTGGDEALKHLAGVTREVMRPQDQLARYGGEEFVIILPDTALAQGVEAMARLQRELTTRYFLKDQEKVLITFSA
GVAQVAPQETTVEAIRRADQGMYLAKRSGKNRVMAA

Sequences:

>Translated_516_residues
MADRPPSEIARETLKQLAARRLVPTPDNYQALYEEIAGTRTAPTFPDAQLRHILRVLPGQTPAQKRLLGQFETAIEQADW
SMLQGVLVGYANLGLHAGGATPVETAPAPLTGGPTGTATVLPEDMAEQLARLVDNTLPALGEDDARVHELAAQLTHFLRQ
PGPPVSTLVLMLNNFSYRLSFATEDQAAIRAGLLTLLHMVFENISVLSKDDHWLQGQAEALKAASTPPLTLRRLDDVQRR
LKDVIFKQNEARERSVQAQQQMKELLATFIERLAQITASSSTYHEKMERCAEQIGQATSLDQITPLLEEVMGATRAMALD
TRLTRDELQELRERSEARHAEIAKLQEELDRASAQARHDPLTGSLNRKGLDEALEREVARALRNDAPLCVALLDLDNFKA
INDRLGHTGGDEALKHLAGVTREVMRPQDQLARYGGEEFVIILPDTALAQGVEAMARLQRELTTRYFLKDQEKVLITFSA
GVAQVAPQETTVEAIRRADQGMYLAKRSGKNRVMAA
>Mature_515_residues
ADRPPSEIARETLKQLAARRLVPTPDNYQALYEEIAGTRTAPTFPDAQLRHILRVLPGQTPAQKRLLGQFETAIEQADWS
MLQGVLVGYANLGLHAGGATPVETAPAPLTGGPTGTATVLPEDMAEQLARLVDNTLPALGEDDARVHELAAQLTHFLRQP
GPPVSTLVLMLNNFSYRLSFATEDQAAIRAGLLTLLHMVFENISVLSKDDHWLQGQAEALKAASTPPLTLRRLDDVQRRL
KDVIFKQNEARERSVQAQQQMKELLATFIERLAQITASSSTYHEKMERCAEQIGQATSLDQITPLLEEVMGATRAMALDT
RLTRDELQELRERSEARHAEIAKLQEELDRASAQARHDPLTGSLNRKGLDEALEREVARALRNDAPLCVALLDLDNFKAI
NDRLGHTGGDEALKHLAGVTREVMRPQDQLARYGGEEFVIILPDTALAQGVEAMARLQRELTTRYFLKDQEKVLITFSAG
VAQVAPQETTVEAIRRADQGMYLAKRSGKNRVMAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=39.5209580838323, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=34.9693251533742, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=33.5294117647059, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=36.7088607594937, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=35.5421686746988, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=29.3785310734463, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788381, Length=174, Percent_Identity=28.735632183908, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=31.6129032258064, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=30.5194805194805, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.2817679558011, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788956, Length=205, Percent_Identity=29.7560975609756, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=32.5, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 57053; Mature: 56922

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRPPSEIARETLKQLAARRLVPTPDNYQALYEEIAGTRTAPTFPDAQLRHILRVLPGQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
TPAQKRLLGQFETAIEQADWSMLQGVLVGYANLGLHAGGATPVETAPAPLTGGPTGTATV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCEE
LPEDMAEQLARLVDNTLPALGEDDARVHELAAQLTHFLRQPGPPVSTLVLMLNNFSYRLS
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEE
FATEDQAAIRAGLLTLLHMVFENISVLSKDDHWLQGQAEALKAASTPPLTLRRLDDVQRR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCHHHHHHHHHHHH
LKDVIFKQNEARERSVQAQQQMKELLATFIERLAQITASSSTYHEKMERCAEQIGQATSL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
DQITPLLEEVMGATRAMALDTRLTRDELQELRERSEARHAEIAKLQEELDRASAQARHDP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTGSLNRKGLDEALEREVARALRNDAPLCVALLDLDNFKAINDRLGHTGGDEALKHLAGV
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHH
TREVMRPQDQLARYGGEEFVIILPDTALAQGVEAMARLQRELTTRYFLKDQEKVLITFSA
HHHHHCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GVAQVAPQETTVEAIRRADQGMYLAKRSGKNRVMAA
CHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
ADRPPSEIARETLKQLAARRLVPTPDNYQALYEEIAGTRTAPTFPDAQLRHILRVLPGQ
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
TPAQKRLLGQFETAIEQADWSMLQGVLVGYANLGLHAGGATPVETAPAPLTGGPTGTATV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCCCCCCEE
LPEDMAEQLARLVDNTLPALGEDDARVHELAAQLTHFLRQPGPPVSTLVLMLNNFSYRLS
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEE
FATEDQAAIRAGLLTLLHMVFENISVLSKDDHWLQGQAEALKAASTPPLTLRRLDDVQRR
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHCCCCCHHHHHHHHHHHH
LKDVIFKQNEARERSVQAQQQMKELLATFIERLAQITASSSTYHEKMERCAEQIGQATSL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCH
DQITPLLEEVMGATRAMALDTRLTRDELQELRERSEARHAEIAKLQEELDRASAQARHDP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LTGSLNRKGLDEALEREVARALRNDAPLCVALLDLDNFKAINDRLGHTGGDEALKHLAGV
CCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHH
TREVMRPQDQLARYGGEEFVIILPDTALAQGVEAMARLQRELTTRYFLKDQEKVLITFSA
HHHHHCCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GVAQVAPQETTVEAIRRADQGMYLAKRSGKNRVMAA
CHHHHCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA