Click here to switch to the map view.

The map label for this gene is yjbJ [H]

Identifier: 120610028

GI number: 120610028

Start: 1466947

End: 1467792

Strand: Direct

Name: yjbJ [H]

Synonym: Aave_1341

Alternate gene names: 120610028

Gene position: 1466947-1467792 (Clockwise)

Preceding gene: 120610027

Following gene: 120610029

Centisome position: 27.41

GC content: 74.35

Gene sequence:

>846_bases
ATGGCGGCGTGGCGCGTGGCGGATGGCACGGCGGTGGTCCGGCTGCGGCAGCAGCTGGCGGCGGGAGCGCGGATGGCCGC
GCTGGGGCTCGCCCTGTGGCTGCCGCTGCAATCCGCCCATGCGGATCTCTGGGCCTACGTGGACGAGCGCGGCGTGACGC
ATTTCGCGGCCGAGCGCATCGACGAGCGCTACAGCCTGTTCTTCCGCGGCGACGTGTTCGACTCCACCAGGGACGGACGC
TCCGCGGGCACGGGCCTCGTGCCGCCCGGCATGGCGCCGGGGGCCACGCCGGAGGGCGCGCGGCGCCTGGCCTATTTCGA
CATCGCGCCCGAGGTCAAGCGCGTGAAGCATTTCCTGCGCGCCTCGGCGAACCGCCACGGGCTCGACTACGAACTGCTGC
AGGCCGTGATCGCGACCGAATCGGGGTTCGATCCGCAGGCGGTGTCGCCGCGCGGCGCGGTGGGGCTCATGCAGGTGATG
CCGGCCACGGCCGAACGCTTCGGCGTGGCGCGCGAGGCCAGGCGTTCGGTGGAGCAGAAGCTCACCGATCCGGGCACGAA
CATCGCGGCCGGCGCGCGCTACCTGAGGCACCTGATCGATCTTTTCGAGGGCCGGCTGGACCTGGCCCTGGCGGCCTACA
ACGCGGGCGAGGGCGCCGTGCAGCGCGCCGGCCGCCGCATTCCCGCCTACCGCGAGACGCAGAACTACGTGAAGAGCGTG
CTGGGGCTCTACGCCCTGCTCAAGCCCGCGGCGGTCGCCCCGGTGCCCGTGGCGGCGGGGCGCGGCGCCGCGGCCGGCCG
GGTGCGCATGGAGCTGGCGGCGCCCGTGGTCCAGGGCGCGCCCTGA

Upstream 100 bases:

>100_bases
CGAGGGCCTCATCACCAAGCTCATGGGCAAGGGAGAGGCGGCCGCGCGGCGCGAGCTGATGGAACTGCACGGCGACGCCG
TGGAAGTGGATATCTGAGCC

Downstream 100 bases:

>100_bases
TCTTTCGAACGACACCGCGGCGCACCCTCCGCCGCGTTCCTCTTTCCATTCTTTCCGACGCATGAGCGACAACGACCAAC
CCACCCTCGACTTCTCCCAG

Product: lytic transglycosylase catalytic subunit

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAAWRVADGTAVVRLRQQLAAGARMAALGLALWLPLQSAHADLWAYVDERGVTHFAAERIDERYSLFFRGDVFDSTRDGR
SAGTGLVPPGMAPGATPEGARRLAYFDIAPEVKRVKHFLRASANRHGLDYELLQAVIATESGFDPQAVSPRGAVGLMQVM
PATAERFGVAREARRSVEQKLTDPGTNIAAGARYLRHLIDLFEGRLDLALAAYNAGEGAVQRAGRRIPAYRETQNYVKSV
LGLYALLKPAAVAPVPVAAGRGAAAGRVRMELAAPVVQGAP

Sequences:

>Translated_281_residues
MAAWRVADGTAVVRLRQQLAAGARMAALGLALWLPLQSAHADLWAYVDERGVTHFAAERIDERYSLFFRGDVFDSTRDGR
SAGTGLVPPGMAPGATPEGARRLAYFDIAPEVKRVKHFLRASANRHGLDYELLQAVIATESGFDPQAVSPRGAVGLMQVM
PATAERFGVAREARRSVEQKLTDPGTNIAAGARYLRHLIDLFEGRLDLALAAYNAGEGAVQRAGRRIPAYRETQNYVKSV
LGLYALLKPAAVAPVPVAAGRGAAAGRVRMELAAPVVQGAP
>Mature_280_residues
AAWRVADGTAVVRLRQQLAAGARMAALGLALWLPLQSAHADLWAYVDERGVTHFAAERIDERYSLFFRGDVFDSTRDGRS
AGTGLVPPGMAPGATPEGARRLAYFDIAPEVKRVKHFLRASANRHGLDYELLQAVIATESGFDPQAVSPRGAVGLMQVMP
ATAERFGVAREARRSVEQKLTDPGTNIAAGARYLRHLIDLFEGRLDLALAAYNAGEGAVQRAGRRIPAYRETQNYVKSVL
GLYALLKPAAVAPVPVAAGRGAAAGRVRMELAAPVVQGAP

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082441, Length=124, Percent_Identity=38.7096774193548, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786405, Length=167, Percent_Identity=28.1437125748503, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 30094; Mature: 29963

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAWRVADGTAVVRLRQQLAAGARMAALGLALWLPLQSAHADLWAYVDERGVTHFAAERI
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DERYSLFFRGDVFDSTRDGRSAGTGLVPPGMAPGATPEGARRLAYFDIAPEVKRVKHFLR
HHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHH
ASANRHGLDYELLQAVIATESGFDPQAVSPRGAVGLMQVMPATAERFGVAREARRSVEQK
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDPGTNIAAGARYLRHLIDLFEGRLDLALAAYNAGEGAVQRAGRRIPAYRETQNYVKSV
HCCCCCCHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHH
LGLYALLKPAAVAPVPVAAGRGAAAGRVRMELAAPVVQGAP
HHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAWRVADGTAVVRLRQQLAAGARMAALGLALWLPLQSAHADLWAYVDERGVTHFAAERI
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
DERYSLFFRGDVFDSTRDGRSAGTGLVPPGMAPGATPEGARRLAYFDIAPEVKRVKHFLR
HHHHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHH
ASANRHGLDYELLQAVIATESGFDPQAVSPRGAVGLMQVMPATAERFGVAREARRSVEQK
HCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTDPGTNIAAGARYLRHLIDLFEGRLDLALAAYNAGEGAVQRAGRRIPAYRETQNYVKSV
HCCCCCCHHHHHHHHHHHHHHHCCHHHHEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHH
LGLYALLKPAAVAPVPVAAGRGAAAGRVRMELAAPVVQGAP
HHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]