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The map label for this gene is dusC [H]

Identifier: 120609942

GI number: 120609942

Start: 1377993

End: 1378964

Strand: Direct

Name: dusC [H]

Synonym: Aave_1255

Alternate gene names: 120609942

Gene position: 1377993-1378964 (Clockwise)

Preceding gene: 120609940

Following gene: 120609944

Centisome position: 25.74

GC content: 73.66

Gene sequence:

>972_bases
ATGTCCCAACCTCCCCTGCTGCTGCTCGCCCCCATGGAGGGGCTTCTCGACTTCGTCCTGCGCGACGTGCTGACGCGCGT
GGGCGGCGTGGACCGCTGTGTCTCCGAATTCATCCGCGTGACCGGCACGCTGCTGCCGGACAAGGTGTTCCTGCGCTACA
TCCCCGAGCTGCGCAACGGAGGGCGCACGGCCGCAGGGGTGCCGGTGCGTGCCCAGCTGCTGGGATCGGACCCTTCCAGC
ATGGCAGAGAATGCGGCGCGGCTGGCCGCGCTGGGGCCGGAGGGCATTGACCTGAATTTCGGCTGTCCGGCCAAGGTGGT
GAACCGCCACGGCGGTGGTGCCGCGCTGCTGCGCGAGCCCGAGCGCATCGCCGCCGTGGTGTCCGCGGTGCGCCGGGCGG
TGCCGCCGGAGATGCCGGTGTCCGCCAAGATGCGGCTGGGCTACGACGACACGTCGCTGGCCCGCGAGTGCGCCCAGGCG
GCGGAGGCCGGCGGAGCCTGCGAACTGGTGGTGCATGCCCGCACCAAGGCCGACGGCTACCGCCCGCCCGCCTACTGGGA
GCTCGTCGCGCCGCTGCGCGCGGCCGTGCGCATTCCCGTGATCGCGAATGGCGAGATCTGGACGGTGGAGGACGCGATGC
GCTGCCGGGCGCTCTCGGGCGGCGATGCCCTCATGCTGGGCCGCGGCATCGTGGCGGACCCGGGCCTGGCCCTGGCCATC
CGCGCGGCCGAGGCGGGCCGTGCCCATGCCCCGGCGCTCGCCTGGGAGGCGCTGCTGCCGCAACTGGGCGTGTTCTGGCG
CACGGTCTGCGAAGACCTGGAGCCGCGCCAGCGCGCGGGGCGGCTCAAGCAGTGGCTCAACCTGCTGCGCCGGCGCTTTC
CCGAGGCGGAGGCCGCCTACCAGCAGGTGCGCACGGTCACCGACCAGCGCACCATCACCGCCTGGGTGGAGGCGCAGACG
GGCGGGGCGTGA

Upstream 100 bases:

>100_bases
GCGGAGAGGGCGGGACATTGGCGACAATGGAAGGCTGCATTCCACCGCACTCCGATGACGCCGCGATGACGCGGCCTCCG
GTGCGGGCGCCCCTGCCCCG

Downstream 100 bases:

>100_bases
GGCCCTGAGCGTTGCCAGAGGGCCTGCCTACAGCAGATGGTCCGGCGCCAGGGGCCCCAGCAGCCGCAGCAGCATCCGCA
GGCCCAGGCTGGCGTCGGGC

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSQPPLLLLAPMEGLLDFVLRDVLTRVGGVDRCVSEFIRVTGTLLPDKVFLRYIPELRNGGRTAAGVPVRAQLLGSDPSS
MAENAARLAALGPEGIDLNFGCPAKVVNRHGGGAALLREPERIAAVVSAVRRAVPPEMPVSAKMRLGYDDTSLARECAQA
AEAGGACELVVHARTKADGYRPPAYWELVAPLRAAVRIPVIANGEIWTVEDAMRCRALSGGDALMLGRGIVADPGLALAI
RAAEAGRAHAPALAWEALLPQLGVFWRTVCEDLEPRQRAGRLKQWLNLLRRRFPEAEAAYQQVRTVTDQRTITAWVEAQT
GGA

Sequences:

>Translated_323_residues
MSQPPLLLLAPMEGLLDFVLRDVLTRVGGVDRCVSEFIRVTGTLLPDKVFLRYIPELRNGGRTAAGVPVRAQLLGSDPSS
MAENAARLAALGPEGIDLNFGCPAKVVNRHGGGAALLREPERIAAVVSAVRRAVPPEMPVSAKMRLGYDDTSLARECAQA
AEAGGACELVVHARTKADGYRPPAYWELVAPLRAAVRIPVIANGEIWTVEDAMRCRALSGGDALMLGRGIVADPGLALAI
RAAEAGRAHAPALAWEALLPQLGVFWRTVCEDLEPRQRAGRLKQWLNLLRRRFPEAEAAYQQVRTVTDQRTITAWVEAQT
GGA
>Mature_322_residues
SQPPLLLLAPMEGLLDFVLRDVLTRVGGVDRCVSEFIRVTGTLLPDKVFLRYIPELRNGGRTAAGVPVRAQLLGSDPSSM
AENAARLAALGPEGIDLNFGCPAKVVNRHGGGAALLREPERIAAVVSAVRRAVPPEMPVSAKMRLGYDDTSLARECAQAA
EAGGACELVVHARTKADGYRPPAYWELVAPLRAAVRIPVIANGEIWTVEDAMRCRALSGGDALMLGRGIVADPGLALAIR
AAEAGRAHAPALAWEALLPQLGVFWRTVCEDLEPRQRAGRLKQWLNLLRRRFPEAEAAYQQVRTVTDQRTITAWVEAQTG
GA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=30.3030303030303, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=236, Percent_Identity=28.3898305084746, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI239788483, Length=173, Percent_Identity=27.7456647398844, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI239788462, Length=173, Percent_Identity=27.7456647398844, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI8923374, Length=172, Percent_Identity=29.0697674418605, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788462, Length=313, Percent_Identity=44.7284345047923, Blast_Score=261, Evalue=3e-71,
Organism=Escherichia coli, GI1789660, Length=233, Percent_Identity=35.6223175965665, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693211, Length=186, Percent_Identity=31.1827956989247, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=239, Percent_Identity=30.9623430962343, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.7142857142857, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=173, Percent_Identity=29.4797687861272, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=26.7123287671233, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921524, Length=176, Percent_Identity=34.6590909090909, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585320, Length=232, Percent_Identity=30.1724137931034, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24580595, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920448, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45549423, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34745; Mature: 34613

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPPLLLLAPMEGLLDFVLRDVLTRVGGVDRCVSEFIRVTGTLLPDKVFLRYIPELRNG
CCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GRTAAGVPVRAQLLGSDPSSMAENAARLAALGPEGIDLNFGCPAKVVNRHGGGAALLREP
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHCCH
ERIAAVVSAVRRAVPPEMPVSAKMRLGYDDTSLARECAQAAEAGGACELVVHARTKADGY
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
RPPAYWELVAPLRAAVRIPVIANGEIWTVEDAMRCRALSGGDALMLGRGIVADPGLALAI
CCCHHHHHHHHHHHHEECCEEECCCEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
RAAEAGRAHAPALAWEALLPQLGVFWRTVCEDLEPRQRAGRLKQWLNLLRRRFPEAEAAY
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
QQVRTVTDQRTITAWVEAQTGGA
HHHHHHHCCCEEEEEEEECCCCC
>Mature Secondary Structure 
SQPPLLLLAPMEGLLDFVLRDVLTRVGGVDRCVSEFIRVTGTLLPDKVFLRYIPELRNG
CCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
GRTAAGVPVRAQLLGSDPSSMAENAARLAALGPEGIDLNFGCPAKVVNRHGGGAALLREP
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHCCH
ERIAAVVSAVRRAVPPEMPVSAKMRLGYDDTSLARECAQAAEAGGACELVVHARTKADGY
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
RPPAYWELVAPLRAAVRIPVIANGEIWTVEDAMRCRALSGGDALMLGRGIVADPGLALAI
CCCHHHHHHHHHHHHEECCEEECCCEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCEEEE
RAAEAGRAHAPALAWEALLPQLGVFWRTVCEDLEPRQRAGRLKQWLNLLRRRFPEAEAAY
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
QQVRTVTDQRTITAWVEAQTGGA
HHHHHHHCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]