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The map label for this gene is dsbC [H]

Identifier: 120609578

GI number: 120609578

Start: 960917

End: 961657

Strand: Reverse

Name: dsbC [H]

Synonym: Aave_0884

Alternate gene names: 120609578

Gene position: 961657-960917 (Counterclockwise)

Preceding gene: 120609579

Following gene: 120609577

Centisome position: 17.97

GC content: 67.48

Gene sequence:

>741_bases
ATGAAACTGATCCCCGCCCTGCTCGCCGGCGCCGCCGCGCTCACCCTCGGCCTCGGCGCCCACGCCCAGGAATCGGCGAT
CCGCAAGGCCCTGGGCAGCCGCATCCCGCAGCTGCAGAACATCGACGAGGTGCGCGCCACGCCCATGCAGGGCCTGTACG
AAGTGCGCATCGGCACCGATGTGTTCTACACCGACGCCAAGGGCAACTACCTCATCCAGGGCGAACTGATCGACACCAAG
GCACGCCGCAACCTCACCGAGGACCGCATCAACAAGCTCACGGCGGTGGATTTCGCCTCCCTGCCGTTCCAGGACGCGTT
CACCATCGTGCGCGGCAACGGCCAGCGCAAGCTCGCCGTGTTCGAGGACCCGAACTGCGGCTACTGCAAGCGCTTCGAGA
AGGACCTGCAGAACGTGGACAACGTGACGGTCTATCTCTTCCTCTATCCCATCCTGAGCCCCGATTCGGCCGAGAAGTCG
CGCAACATCTGGTGCTCCAAGGACCGCGCGGCGGCATGGCAGGACCACATGGTGCGCGACAAGACCACGCCGGCCGCGAG
CTGCGACACGGCCGCCGTGCAGCGCAACCTGGCCTTCGGCAAGAAGCACAAGATCACCGGCACGCCCACGCTGATCTTCA
CCGACGGCTCGCGCGTTCCCGGCGCCATCGACGCGCGCGAGGTGGAAAAGCGCCTCGCCGACGCGGGATCGGGCGCCGGC
CCGGCCCCCGCCACGAACTGA

Upstream 100 bases:

>100_bases
GCCAGGCCATGGGCCTGGGCTGACGGCCCGCCGCATCCCGGGATGCGGCGGCCGCCGTGCCGCCCATTCCCCAGAGATTC
ACGCATCACGAAGGAGCACC

Downstream 100 bases:

>100_bases
TCCTTTTCCCGCCCCTCCCGGATACCGCATGGCCGCCCACCGATCGCACGCCCCCGCCGTCCATTACCGCGTGGAAGCCG
CGCAGTTGCACGCGCGCATC

Product: putative thiol:disulfide interchange protein (periplasmic)

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKLIPALLAGAAALTLGLGAHAQESAIRKALGSRIPQLQNIDEVRATPMQGLYEVRIGTDVFYTDAKGNYLIQGELIDTK
ARRNLTEDRINKLTAVDFASLPFQDAFTIVRGNGQRKLAVFEDPNCGYCKRFEKDLQNVDNVTVYLFLYPILSPDSAEKS
RNIWCSKDRAAAWQDHMVRDKTTPAASCDTAAVQRNLAFGKKHKITGTPTLIFTDGSRVPGAIDAREVEKRLADAGSGAG
PAPATN

Sequences:

>Translated_246_residues
MKLIPALLAGAAALTLGLGAHAQESAIRKALGSRIPQLQNIDEVRATPMQGLYEVRIGTDVFYTDAKGNYLIQGELIDTK
ARRNLTEDRINKLTAVDFASLPFQDAFTIVRGNGQRKLAVFEDPNCGYCKRFEKDLQNVDNVTVYLFLYPILSPDSAEKS
RNIWCSKDRAAAWQDHMVRDKTTPAASCDTAAVQRNLAFGKKHKITGTPTLIFTDGSRVPGAIDAREVEKRLADAGSGAG
PAPATN
>Mature_246_residues
MKLIPALLAGAAALTLGLGAHAQESAIRKALGSRIPQLQNIDEVRATPMQGLYEVRIGTDVFYTDAKGNYLIQGELIDTK
ARRNLTEDRINKLTAVDFASLPFQDAFTIVRGNGQRKLAVFEDPNCGYCKRFEKDLQNVDNVTVYLFLYPILSPDSAEKS
RNIWCSKDRAAAWQDHMVRDKTTPAASCDTAAVQRNLAFGKKHKITGTPTLIFTDGSRVPGAIDAREVEKRLADAGSGAG
PAPATN

Specific function: Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process [H]

COG id: COG1651

COG function: function code O; Protein-disulfide isomerase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789260, Length=221, Percent_Identity=28.5067873303167, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018950
- InterPro:   IPR009094
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF10411 DsbC_N [H]

EC number: NA

Molecular weight: Translated: 26808; Mature: 26808

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIPALLAGAAALTLGLGAHAQESAIRKALGSRIPQLQNIDEVRATPMQGLYEVRIGTD
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCE
VFYTDAKGNYLIQGELIDTKARRNLTEDRINKLTAVDFASLPFQDAFTIVRGNGQRKLAV
EEEECCCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEE
FEDPNCGYCKRFEKDLQNVDNVTVYLFLYPILSPDSAEKSRNIWCSKDRAAAWQDHMVRD
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHCCCEECCCCHHHHHHHHHCC
KTTPAASCDTAAVQRNLAFGKKHKITGTPTLIFTDGSRVPGAIDAREVEKRLADAGSGAG
CCCCCCCCHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCC
PAPATN
CCCCCC
>Mature Secondary Structure
MKLIPALLAGAAALTLGLGAHAQESAIRKALGSRIPQLQNIDEVRATPMQGLYEVRIGTD
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCE
VFYTDAKGNYLIQGELIDTKARRNLTEDRINKLTAVDFASLPFQDAFTIVRGNGQRKLAV
EEEECCCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEE
FEDPNCGYCKRFEKDLQNVDNVTVYLFLYPILSPDSAEKSRNIWCSKDRAAAWQDHMVRD
EECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHCCCEECCCCHHHHHHHHHCC
KTTPAASCDTAAVQRNLAFGKKHKITGTPTLIFTDGSRVPGAIDAREVEKRLADAGSGAG
CCCCCCCCHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCC
PAPATN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]