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The map label for this gene is miaB

Identifier: 120609569

GI number: 120609569

Start: 951328

End: 952677

Strand: Reverse

Name: miaB

Synonym: Aave_0875

Alternate gene names: 120609569

Gene position: 952677-951328 (Counterclockwise)

Preceding gene: 120609570

Following gene: 120609568

Centisome position: 17.8

GC content: 65.7

Gene sequence:

>1350_bases
ATGGCCAAAAAAGTCTTCGTCAAAACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTGGACGTGCTCAA
CGCCGCGCAGGGCTACGAGCCCACGCAGGACGTGGAGGAAGCCGACCTGATCCTCTTCAATACCTGCTCCGTGCGCGAGA
AGGCGCAGGAAAAGGTCTTCAGCGACCTGGGCCGCATCAAGCACCTCAAGGCCCGCGGAGTGAAGATCGGCGTGGGCGGC
TGCGTGGCCAGCCAGGAGGGCGACGAGATCATCAAGCGCGCGCCCTACGTCGATGTGGTCTTCGGCCCCCAGACCCTGCA
CCGCCTGCCCGAACTGCTGGCGCAGCGCGAGGCCCTGGCACGCCCGCAGGTGGACATCAGCTTCCCCGAGATCGAGAAGT
TCGACCACCTGCCGCCGGCGCGCGTCGAGGGCGCGAGCGCCTTCGTCTCCATCATGGAAGGCTGCAGCAAGTACTGCAGC
TACTGCGTGGTGCCCTACACCCGCGGGGAGGAAGTCAGCCGCCCGTTCGACGACGTGCTGGTGGAGGTCGCGGGCCTGGC
CGACCAGGGCGTGAAGGAGGTCACCCTGCTCGGCCAGAACGTGAACGCCTACCTGGGCAAAATGGGCGGCACGGCCGAGG
TGGCGGATTTCGCCCTGCTGCTGGAATACGTGGCCGACATCACGGGCATCGAGCGCATCCGCTTCACCACCAGCCACCCC
AACGAATTCACGCAGCGCCTGATCGATGCCTACGCGCGCATCCCGAAGCTGGTCAGCCACCTGCACCTGCCCGTGCAGCA
CGGCTCCGACCGCATCCTGATGGCCATGAAGCGCGGCTACACGGCCATGGAATACAAGAGCACCATCCGTAAGCTGCGCG
CCATCCGGCCCGACCTGGCCATGAGCAGCGATTTCATCGTGGGTTTCCCCGGCGAGACCGAGGACGACTTCGGCAAGATG
ATGAAGCTCATCGACGACATCCACTTCGACAACAGCTTCAGCTTCATCTTCAGCCCCCGCCCCGGCACGCCCGCCGCCAG
CCTGCACGACGACACGCCGCACGAGGTCAAGCTGCGCCGCCTGCAGGAGCTGCAGGCCGTCATCAACGCCAACATCAAGT
CGATCAGCGAAAGCCGCGTGGGCACCGTGCAGCGCATCCTGGTGGAAGGCGCGTCCAAACGCGACGGCAGCGAACTCATG
GGCCGCACCGAATGCAACCGCGTGGTCAACTTCGCGGGCCACCCGCGGCTCGTCGGGCAGATGGTCGATGTCACCATCAC
CGAAGCCAAGGCCTACACGCTGCGCGGCGAGGTGGTCACGGCGGACCACGGCGCGCTGGCCGCGCACTGA

Upstream 100 bases:

>100_bases
TCCCGCCTACGCCTTCTCCTGATTGCTCCCGGCCGCCCGCGCACGGCCCGCACCCGGCGCCCGCACGGCGCCGCCACTCC
CTTTTCCGGAATTTCCCGCC

Downstream 100 bases:

>100_bases
GGCAGAGGGCCGCATGGGCTTCGCCGCGCGCCTCTTCCGCCGCCAGCAGGGCGTCGCCGCCCTCGGGGGGCTGTCGTTCC
AGCAGATGCTGCTGCTGGCC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVFSDLGRIKHLKARGVKIGVGG
CVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALARPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCS
YCVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHP
NEFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFGKM
MKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRRLQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELM
GRTECNRVVNFAGHPRLVGQMVDVTITEAKAYTLRGEVVTADHGALAAH

Sequences:

>Translated_449_residues
MAKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVFSDLGRIKHLKARGVKIGVGG
CVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALARPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCS
YCVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHP
NEFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFGKM
MKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRRLQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELM
GRTECNRVVNFAGHPRLVGQMVDVTITEAKAYTLRGEVVTADHGALAAH
>Mature_448_residues
AKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVFSDLGRIKHLKARGVKIGVGGC
VASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALARPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSY
CVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHPN
EFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFGKMM
KLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRRLQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELMG
RTECNRVVNFAGHPRLVGQMVDVTITEAKAYTLRGEVVTADHGALAAH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=37.9668049792531, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=37.9668049792531, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI93277076, Length=459, Percent_Identity=28.9760348583878, Blast_Score=182, Evalue=7e-46,
Organism=Escherichia coli, GI1786882, Length=442, Percent_Identity=63.3484162895928, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1787057, Length=455, Percent_Identity=28.7912087912088, Blast_Score=168, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=35.5460385438972, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=35.3741496598639, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=30.1098901098901, Blast_Score=161, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ACIAC (A1TKI5)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969247.1
- ProteinModelPortal:   A1TKI5
- SMR:   A1TKI5
- STRING:   A1TKI5
- GeneID:   4667259
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0875
- NMPDR:   fig|397945.5.peg.760
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A1TKI5
- ProtClustDB:   PRK14325
- BioCyc:   AAVE397945:AAVE_0875-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49704; Mature: 49573

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVF
CCCEEEEHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRIKHLKARGVKIGVGGCVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALA
HHHHHHHHHHHCCEEEECCCEECCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHC
RPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHH
VEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHP
HHHHHHHHCCCHHHHEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHEEEECCCC
NEFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCC
MSSDFIVGFPGETEDDFGKMMKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRR
CCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
LQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELMGRTECNRVVNFAGHPRLVGQ
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
MVDVTITEAKAYTLRGEVVTADHGALAAH
HHEEEEECCEEEEEECCEEECCCCCCCCC
>Mature Secondary Structure 
AKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVF
CCEEEEHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRIKHLKARGVKIGVGGCVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALA
HHHHHHHHHHHCCEEEECCCEECCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHC
RPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL
CCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHHH
VEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHP
HHHHHHHHCCCHHHHEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHEEEECCCC
NEFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCC
MSSDFIVGFPGETEDDFGKMMKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRR
CCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
LQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELMGRTECNRVVNFAGHPRLVGQ
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
MVDVTITEAKAYTLRGEVVTADHGALAAH
HHEEEEECCEEEEEECCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA