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The map label for this gene is ysgA [C]

Identifier: 120609024

GI number: 120609024

Start: 353322

End: 354011

Strand: Direct

Name: ysgA [C]

Synonym: Aave_0321

Alternate gene names: 120609024

Gene position: 353322-354011 (Clockwise)

Preceding gene: 120609017

Following gene: 120609031

Centisome position: 6.6

GC content: 70.43

Gene sequence:

>690_bases
ATGGGCAGTTTCGTGGATCTGGCCTCGGCAGATGGTTTCAAGGTGCCAGCCTGGGTGGCGCGGCCCGAAGGCCGGGCGCG
CGGCGCCGTGGTGGTCCTGCAGGAGATCTTCGGCGTCAACTCGCACATCCGCTCCGTGGCCGACCGCTATGCGGCCGCGG
GCTACCTCGCGGTGGCGCCCGCCACCTTCGCGCGCGTGCAACCCGGCGTGGGACTGGGCTACACCGACGACGACATGAAG
GAAGGTTTCGGCCTGAAGCAGCAAGTGGAAGCGCTGCCGGGCGCGGGCGTGCTGCCCGACATCCAGGCGGCCATCGACCA
TGCGGCGCAGCAGAGCGGCGGCAAGGTCGGCATCGTGGGCTACTGCTGGGGCGGCCTGCTGTCCTGGCGGGCGGCGTGCG
AACTCCGGGGCCTGTCGGCCGCGGCCGTGTACTACGGCGGCGGCATCACGGGCGGCCAGGAAGTGGCCCGCACCCCGCGC
TGCCCGGTGATCGCGCACTTCGGCAAGCGCGACCACTACATCCCGCTGGAAGGCGTGGAGGACTTCCGCAAGGCACACCC
GGACGTCGAGGTGCATGTCTATGAAGCCGACCACGGCTTCAACTGCGACCAGCGCGGCTCCTACGACGCACCGTCCGCGG
ACCTGGCGCGCGAACGCACGCTGGCGTTCTTCGCGAAGTACCTGGGCTGA

Upstream 100 bases:

>100_bases
CAGCACTGCCGCGCCCGGCGCGGTCCGGCTGCTGCGATCGGTACCCGCCGCTCCCTCCCCCCGGGGGCGCTCAACCCGTT
CATATCGAAGGAGTGACAAC

Downstream 100 bases:

>100_bases
GGGCTACCGACGCCGTGGAACCGGCTCTGCCGGGCCACTGGCGTCGTCCCCCCTGCAGCCGCTCAGGGGAGAGGTCCAGT
CAATGCGCACCCGCCGCCGC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MGSFVDLASADGFKVPAWVARPEGRARGAVVVLQEIFGVNSHIRSVADRYAAAGYLAVAPATFARVQPGVGLGYTDDDMK
EGFGLKQQVEALPGAGVLPDIQAAIDHAAQQSGGKVGIVGYCWGGLLSWRAACELRGLSAAAVYYGGGITGGQEVARTPR
CPVIAHFGKRDHYIPLEGVEDFRKAHPDVEVHVYEADHGFNCDQRGSYDAPSADLARERTLAFFAKYLG

Sequences:

>Translated_229_residues
MGSFVDLASADGFKVPAWVARPEGRARGAVVVLQEIFGVNSHIRSVADRYAAAGYLAVAPATFARVQPGVGLGYTDDDMK
EGFGLKQQVEALPGAGVLPDIQAAIDHAAQQSGGKVGIVGYCWGGLLSWRAACELRGLSAAAVYYGGGITGGQEVARTPR
CPVIAHFGKRDHYIPLEGVEDFRKAHPDVEVHVYEADHGFNCDQRGSYDAPSADLARERTLAFFAKYLG
>Mature_228_residues
GSFVDLASADGFKVPAWVARPEGRARGAVVVLQEIFGVNSHIRSVADRYAAAGYLAVAPATFARVQPGVGLGYTDDDMKE
GFGLKQQVEALPGAGVLPDIQAAIDHAAQQSGGKVGIVGYCWGGLLSWRAACELRGLSAAAVYYGGGITGGQEVARTPRC
PVIAHFGKRDHYIPLEGVEDFRKAHPDVEVHVYEADHGFNCDQRGSYDAPSADLARERTLAFFAKYLG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=238, Percent_Identity=34.0336134453782, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24353; Mature: 24221

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSFVDLASADGFKVPAWVARPEGRARGAVVVLQEIFGVNSHIRSVADRYAAAGYLAVAP
CCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHEECC
ATFARVQPGVGLGYTDDDMKEGFGLKQQVEALPGAGVLPDIQAAIDHAAQQSGGKVGIVG
HHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHH
YCWGGLLSWRAACELRGLSAAAVYYGGGITGGQEVARTPRCPVIAHFGKRDHYIPLEGVE
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCHH
DFRKAHPDVEVHVYEADHGFNCDQRGSYDAPSADLARERTLAFFAKYLG
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GSFVDLASADGFKVPAWVARPEGRARGAVVVLQEIFGVNSHIRSVADRYAAAGYLAVAP
CCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHEECC
ATFARVQPGVGLGYTDDDMKEGFGLKQQVEALPGAGVLPDIQAAIDHAAQQSGGKVGIVG
HHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEHH
YCWGGLLSWRAACELRGLSAAAVYYGGGITGGQEVARTPRCPVIAHFGKRDHYIPLEGVE
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHCCCCCCEEEECCCCCCCCCCCCHH
DFRKAHPDVEVHVYEADHGFNCDQRGSYDAPSADLARERTLAFFAKYLG
HHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]