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The map label for this gene is pcaK [H]

Identifier: 120608857

GI number: 120608857

Start: 163588

End: 164913

Strand: Direct

Name: pcaK [H]

Synonym: Aave_0149

Alternate gene names: 120608857

Gene position: 163588-164913 (Clockwise)

Preceding gene: 120608856

Following gene: 120608858

Centisome position: 3.06

GC content: 71.27

Gene sequence:

>1326_bases
ATGACCCCATCCATGGCCGCCGGCATCCCGGCGGCAGACCGCGCCGCCTTCGGGCCGCTGCAATTGCGCGTGACGCTGCT
GTGCGCCATCGTCGCCATGCTCGACGGCTTCGATACCCAGTCCATCGCCTACGTGGCGCCGCGCATCGCGGAAGACTGGG
GCCTCAAGGCCTCCGAGTTCGGCCCCATCTTCGCGGCGGGCCTGTTCGGCCTGATGGCCGGCGCCTTCCTGCTCAGCCCG
GCGGCCGACCGCTGGGGCCGCAAGAAGGTGATCCTGCTGTCCACCTGCATCTTCGGCCTGTTCGCGCTGCTCACGGCCTG
GGCCACCAGCATGAACGAGCTGCTGGCGTACCGTTTCATCACCGGCGTGGGCCTGGGCGCGGCCATGCCCAACATCATCG
CGCTCACCAGCGAATACGCGCCGCAGCGCCTGCGCGCCACGCTGGTCACGGTGATGTTCTGCGGATTCCCGCTGGGCTCC
ACGCTGGGCGGGCTCATTTCCAGCTGGCTCATCGCGCATTTCGACTGGCACTCGGTCTTCGTGCTGGGCGGCGTGCTGCC
CCTGCTGCTGTTGCCCGTGCTGGCCCTGCTGCTGCCCGAATCGCTGCGCTACCTGGCCGCCAGCGGCGCCGCGCCCGCAC
GCTACGAGCCCATCGCGCGCAGTGCCTACCCGGGCGAAGACCCCGCCACGGTGATGGCCGGGCTGCGCGGCGAGGCCGCG
GGCGTGCAGGGCGGCTTTTCGGTGTTCGAGCTGTTCCGCCACGGCCGCCTGCACACCACCGTGCTGCTCTGGCTCGCGTT
CTTCCTGAACCTGCTGGTCATGTATTTCCTGGTGAACTGGCTGCCCACGCTGCTCAAGAGCGCCGGCCTGCCGCTGGAAA
AAGCCATCCTCTCCACCGCCATGCTCAACCTGGGCGGCGTGGTGGGCGCGCTCGTGCTGGGCCGGCTCATCGACCGCTTC
AGCGCCTACACCGTGCTGGGCGCGGCCTACGCCGCATCGGCCGGTTTCATCGCGCTGATCGCTTTCAGCGGGCAGAACCT
CACGGGCCTGCTGGCCGGCGCCGCGCTCTCGGGCTTCGGCGTGGTGGGCGCGCAGATCGGCTGCAACGCACTGGCCGCCG
CCATCTACCCCACCCATATCCGCGCCACCGGCGTGGGCTGGGCGCTGGGCGTGGGGCGCATCGGCGCCATCGTGGGCCCG
CTGGCGGGCGGCTCGCTATTGGCCGCGGCCTGGACGCCGTCGTCCATCATCCTGGTGGCCGCCGTGCCGGCGGTGCTCGC
AGCCGTGGCGGTGGTGCTGCTCGGGCAGGCGCGGCGCGGCGCCTGA

Upstream 100 bases:

>100_bases
CCGGCAGTCCCACGGGCCAGAGATCCGCGGGCCAGGGTGGGGCACACCACCCGGCACTGGCGGGCCGGGCCTTCCATCCA
TAAGAACGGAGCGAGACAAA

Downstream 100 bases:

>100_bases
GAAGGCATGCCATGCACGACGGCCTTCGCACCTGGACCACCGACGCCGTTCCCCCCGCGCAGCGGGCCGACTACTGGGTC
GGCGCCGTCTGCGACTGCTT

Product: major facilitator superfamily transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTPSMAAGIPAADRAAFGPLQLRVTLLCAIVAMLDGFDTQSIAYVAPRIAEDWGLKASEFGPIFAAGLFGLMAGAFLLSP
AADRWGRKKVILLSTCIFGLFALLTAWATSMNELLAYRFITGVGLGAAMPNIIALTSEYAPQRLRATLVTVMFCGFPLGS
TLGGLISSWLIAHFDWHSVFVLGGVLPLLLLPVLALLLPESLRYLAASGAAPARYEPIARSAYPGEDPATVMAGLRGEAA
GVQGGFSVFELFRHGRLHTTVLLWLAFFLNLLVMYFLVNWLPTLLKSAGLPLEKAILSTAMLNLGGVVGALVLGRLIDRF
SAYTVLGAAYAASAGFIALIAFSGQNLTGLLAGAALSGFGVVGAQIGCNALAAAIYPTHIRATGVGWALGVGRIGAIVGP
LAGGSLLAAAWTPSSIILVAAVPAVLAAVAVVLLGQARRGA

Sequences:

>Translated_441_residues
MTPSMAAGIPAADRAAFGPLQLRVTLLCAIVAMLDGFDTQSIAYVAPRIAEDWGLKASEFGPIFAAGLFGLMAGAFLLSP
AADRWGRKKVILLSTCIFGLFALLTAWATSMNELLAYRFITGVGLGAAMPNIIALTSEYAPQRLRATLVTVMFCGFPLGS
TLGGLISSWLIAHFDWHSVFVLGGVLPLLLLPVLALLLPESLRYLAASGAAPARYEPIARSAYPGEDPATVMAGLRGEAA
GVQGGFSVFELFRHGRLHTTVLLWLAFFLNLLVMYFLVNWLPTLLKSAGLPLEKAILSTAMLNLGGVVGALVLGRLIDRF
SAYTVLGAAYAASAGFIALIAFSGQNLTGLLAGAALSGFGVVGAQIGCNALAAAIYPTHIRATGVGWALGVGRIGAIVGP
LAGGSLLAAAWTPSSIILVAAVPAVLAAVAVVLLGQARRGA
>Mature_440_residues
TPSMAAGIPAADRAAFGPLQLRVTLLCAIVAMLDGFDTQSIAYVAPRIAEDWGLKASEFGPIFAAGLFGLMAGAFLLSPA
ADRWGRKKVILLSTCIFGLFALLTAWATSMNELLAYRFITGVGLGAAMPNIIALTSEYAPQRLRATLVTVMFCGFPLGST
LGGLISSWLIAHFDWHSVFVLGGVLPLLLLPVLALLLPESLRYLAASGAAPARYEPIARSAYPGEDPATVMAGLRGEAAG
VQGGFSVFELFRHGRLHTTVLLWLAFFLNLLVMYFLVNWLPTLLKSAGLPLEKAILSTAMLNLGGVVGALVLGRLIDRFS
AYTVLGAAYAASAGFIALIAFSGQNLTGLLAGAALSGFGVVGAQIGCNALAAAIYPTHIRATGVGWALGVGRIGAIVGPL
AGGSLLAAAWTPSSIILVAAVPAVLAAVAVVLLGQARRGA

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=460, Percent_Identity=25.4347826086956, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI7662270, Length=213, Percent_Identity=27.2300469483568, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI213021148, Length=461, Percent_Identity=25.5965292841649, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI8923870, Length=374, Percent_Identity=27.0053475935829, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20070188, Length=409, Percent_Identity=25.6723716381418, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87081723, Length=414, Percent_Identity=37.6811594202899, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788074, Length=372, Percent_Identity=27.1505376344086, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788068, Length=443, Percent_Identity=24.1534988713318, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082159, Length=397, Percent_Identity=26.1964735516373, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994889, Length=149, Percent_Identity=32.2147651006711, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786229, Length=223, Percent_Identity=26.457399103139, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789207, Length=288, Percent_Identity=24.3055555555556, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082404, Length=380, Percent_Identity=22.6315789473684, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=412, Percent_Identity=23.7864077669903, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640198, Length=478, Percent_Identity=21.7573221757322, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24640196, Length=478, Percent_Identity=21.7573221757322, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24640200, Length=478, Percent_Identity=21.7573221757322, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI161077756, Length=463, Percent_Identity=23.7580993520518, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922874, Length=438, Percent_Identity=20.5479452054795, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45604; Mature: 45472

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSMAAGIPAADRAAFGPLQLRVTLLCAIVAMLDGFDTQSIAYVAPRIAEDWGLKASEF
CCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHC
GPIFAAGLFGLMAGAFLLSPAADRWGRKKVILLSTCIFGLFALLTAWATSMNELLAYRFI
CHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVGLGAAMPNIIALTSEYAPQRLRATLVTVMFCGFPLGSTLGGLISSWLIAHFDWHSVF
HHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
VLGGVLPLLLLPVLALLLPESLRYLAASGAAPARYEPIARSAYPGEDPATVMAGLRGEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHCCCCCCC
GVQGGFSVFELFRHGRLHTTVLLWLAFFLNLLVMYFLVNWLPTLLKSAGLPLEKAILSTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLNLGGVVGALVLGRLIDRFSAYTVLGAAYAASAGFIALIAFSGQNLTGLLAGAALSGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCH
VVGAQIGCNALAAAIYPTHIRATGVGWALGVGRIGAIVGPLAGGSLLAAAWTPSSIILVA
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
AVPAVLAAVAVVLLGQARRGA
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPSMAAGIPAADRAAFGPLQLRVTLLCAIVAMLDGFDTQSIAYVAPRIAEDWGLKASEF
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHC
GPIFAAGLFGLMAGAFLLSPAADRWGRKKVILLSTCIFGLFALLTAWATSMNELLAYRFI
CHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVGLGAAMPNIIALTSEYAPQRLRATLVTVMFCGFPLGSTLGGLISSWLIAHFDWHSVF
HHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
VLGGVLPLLLLPVLALLLPESLRYLAASGAAPARYEPIARSAYPGEDPATVMAGLRGEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHHHHHCCCCCCC
GVQGGFSVFELFRHGRLHTTVLLWLAFFLNLLVMYFLVNWLPTLLKSAGLPLEKAILSTA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLNLGGVVGALVLGRLIDRFSAYTVLGAAYAASAGFIALIAFSGQNLTGLLAGAALSGFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCH
VVGAQIGCNALAAAIYPTHIRATGVGWALGVGRIGAIVGPLAGGSLLAAAWTPSSIILVA
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
AVPAVLAAVAVVLLGQARRGA
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]