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The map label for this gene is maiA [H]

Identifier: 120608856

GI number: 120608856

Start: 162841

End: 163482

Strand: Direct

Name: maiA [H]

Synonym: Aave_0148

Alternate gene names: 120608856

Gene position: 162841-163482 (Clockwise)

Preceding gene: 120608855

Following gene: 120608857

Centisome position: 3.04

GC content: 70.4

Gene sequence:

>642_bases
ATGCAGCTCTACAGCTTTTTCAACAGCTCCACCTCGTACCGGGTGCGCATCGCGCTGGCGCTCAAGGGGCTGCCGGCCGA
CTACCACGGCGTGAACATCCGCACCGGCGAGCACCGCGCGGCGGACTATGCCGAACTGAACGCCGCGCGCGTGGTGCCCA
CGCTGGTGGACGGCGATTTCACGCTGGGCCAATCGATGGCCATCCTCGACTACCTGGACCAGCGCCATCCCGAGCCCCGG
CTGATCCCGCTCGAACCCCGCCAGCGCGCCCGCGTGCTGGAGCTGGCGAATGTGATCGCCTGCGACATGCACCCGGTGAA
CAACCTGCGCATCCTGAAATACCTGCAGGGCCCGCTGGCCTTGAGCGCGGCGCAGAAGGACGCGTGGTACGCCCACTGGG
CGGCCGAGGGTTTCGCTGCCGTGGAGCGGCTGCTGGTGCGGCATGGCGGCGAAGGCCCCTGGTGCTTCGGCGACCAGCCC
ACCCTGGCCGACGTGTGCCTGGTGCCCCAGGTGGCGAATGCGCTGCGCGCGGGCTGCGACCTCGCGCCCCATGCGCGCGT
GATGGCCGTGCACCGGCATGCCGCGCAGCATCCGGCCTTCAGCGCCGCGCGGCCGCAGGCGCAGCCGGATTACCAGCCAT
GA

Upstream 100 bases:

>100_bases
CGAAGGCGTGGGCCCGGTGGTGCGCGGCGACCGCATGGTGGTGGGCGTGGAGCGCCTGGGCGAGCTGGCGGTGGACGTGG
TCTGAGGCGACGGCGACGCC

Downstream 100 bases:

>100_bases
GTTGACCGGCAGTCCCACGGGCCAGAGATCCGCGGGCCAGGGTGGGGCACACCACCCGGCACTGGCGGGCCGGGCCTTCC
ATCCATAAGAACGGAGCGAG

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLYSFFNSSTSYRVRIALALKGLPADYHGVNIRTGEHRAADYAELNAARVVPTLVDGDFTLGQSMAILDYLDQRHPEPR
LIPLEPRQRARVLELANVIACDMHPVNNLRILKYLQGPLALSAAQKDAWYAHWAAEGFAAVERLLVRHGGEGPWCFGDQP
TLADVCLVPQVANALRAGCDLAPHARVMAVHRHAAQHPAFSAARPQAQPDYQP

Sequences:

>Translated_213_residues
MQLYSFFNSSTSYRVRIALALKGLPADYHGVNIRTGEHRAADYAELNAARVVPTLVDGDFTLGQSMAILDYLDQRHPEPR
LIPLEPRQRARVLELANVIACDMHPVNNLRILKYLQGPLALSAAQKDAWYAHWAAEGFAAVERLLVRHGGEGPWCFGDQP
TLADVCLVPQVANALRAGCDLAPHARVMAVHRHAAQHPAFSAARPQAQPDYQP
>Mature_213_residues
MQLYSFFNSSTSYRVRIALALKGLPADYHGVNIRTGEHRAADYAELNAARVVPTLVDGDFTLGQSMAILDYLDQRHPEPR
LIPLEPRQRARVLELANVIACDMHPVNNLRILKYLQGPLALSAAQKDAWYAHWAAEGFAAVERLLVRHGGEGPWCFGDQP
TLADVCLVPQVANALRAGCDLAPHARVMAVHRHAAQHPAFSAARPQAQPDYQP

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=40.0943396226415, Blast_Score=154, Evalue=4e-38,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=37.7358490566038, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=32.5471698113208, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1789624, Length=182, Percent_Identity=29.6703296703297, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=36.0576923076923, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=33.9712918660287, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556142, Length=214, Percent_Identity=35.981308411215, Blast_Score=123, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45553325, Length=213, Percent_Identity=38.0281690140845, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355859, Length=213, Percent_Identity=38.0281690140845, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645375, Length=213, Percent_Identity=38.0281690140845, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21355857, Length=214, Percent_Identity=34.1121495327103, Blast_Score=140, Evalue=7e-34,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23516; Mature: 23516

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLPADYHGVNIRTGEHRAADYAELNAARVVPTLVDGDF
CCCEEECCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCCCHHHHHCHHHHCEEEECCCC
TLGQSMAILDYLDQRHPEPRLIPLEPRQRARVLELANVIACDMHPVNNLRILKYLQGPLA
CCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHH
LSAAQKDAWYAHWAAEGFAAVERLLVRHGGEGPWCFGDQPTLADVCLVPQVANALRAGCD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LAPHARVMAVHRHAAQHPAFSAARPQAQPDYQP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLPADYHGVNIRTGEHRAADYAELNAARVVPTLVDGDF
CCCEEECCCCCCEEEEEEEEEECCCCCCCCCEEECCCCCCCHHHHHCHHHHCEEEECCCC
TLGQSMAILDYLDQRHPEPRLIPLEPRQRARVLELANVIACDMHPVNNLRILKYLQGPLA
CCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHH
LSAAQKDAWYAHWAAEGFAAVERLLVRHGGEGPWCFGDQPTLADVCLVPQVANALRAGCD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LAPHARVMAVHRHAAQHPAFSAARPQAQPDYQP
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]