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The map label for this gene is mdh [H]

Identifier: 120597553

GI number: 120597553

Start: 801499

End: 802434

Strand: Direct

Name: mdh [H]

Synonym: Sputw3181_0722

Alternate gene names: 120597553

Gene position: 801499-802434 (Clockwise)

Preceding gene: 120597551

Following gene: 120597555

Centisome position: 17.02

GC content: 47.76

Gene sequence:

>936_bases
ATGAAAGTTGCTGTACTTGGTGCTGCTGGTGGTATCGGCCAGGCTCTTGCCCTACTGTTAAAAACTCAATTGCCTGCGGG
TTCTAAACTGTCTCTATACGATATCGCACCGGTGACTCCGGGTGTTGCGGTTGACTTGAGCCATATTCCAACTGCGGTTG
AGATCAAAGGTTTTGCTGGTGAAGATCCAACGCCTGCCCTCGTGGGCGCTGACGTTGTACTCATTTCTGCAGGTGTTGCA
CGTAAGCCTGGTATGGATCGTTCAGATCTGTTCAATATCAACGCGGGCATTGTTCGCAATCTGATTGAAAAAGTGGCTGC
GACTTGTCCAAAAGCACTTGTTGGTATCATTACTAACCCTGTGAATACGACAGTTGCTATCGCTGCTGAAGTGATGAAAA
AAGCAGGTGTTTACGATAAGAACCGTCTATTCGGTGTGACCACTCTGGACGTTATCCGTTCTGAAACTTTCATCGCTGAA
TTAAAAGGCTTAAACGTTGCTGACGTTAAAATCAACGTGATCGGCGGCCACAGCGGTGTGACCATTCTGCCATTACTGTC
TCAAGTTGAAGGCGTGACTTTCTCCGATGAAGAAGTCGCTTCTTTAACTAAACGCATTCAAAACGCGGGTACTGAAGTGG
TTGAAGCTAAAGCAGGTGGCGGAAGCGCGACTCTGTCTATGGGCCAAGCGGCTTGCCGTTTCGGTATGTCTCTGGTTCGT
GGTCTGCAAGGCGAAGCGAATGTCGTTGAATGTGCCTACGTTGATGGCGGCAGCGAACACGCTGAATTCTTCGCACAACC
TGTGCTATTAGGCAAAAACGGTATCGAAAAAGTACTGCCTTACGGTGAAGTGAGTGCATTTGAAGCTAACGCCCGTGATT
CTATGTTAGATACGCTGAAAGGCGATATCAAACTAGGCGTTGATTTCGTTAAATAA

Upstream 100 bases:

>100_bases
CGACTAAGGGATAATTGAAATTGTTGTGACATTCCAGTAATGTGACGCCAACGGAAACTAGATCCATGTCACACATATCC
GAGAAATAACGGAGATAACT

Downstream 100 bases:

>100_bases
TCTTTTTAGATTGTGACGAATGAATAACGGAGCCATTGCGCTCCGTTTTTTTATGCCTACATTTTACACTTTCTCAAGCT
AATTCAGTCGATCAGCGGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGVTTLDVIRSETFIAE
LKGLNVADVKINVIGGHSGVTILPLLSQVEGVTFSDEEVASLTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGMSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Sequences:

>Translated_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGVTTLDVIRSETFIAE
LKGLNVADVKINVIGGHSGVTILPLLSQVEGVTFSDEEVASLTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGMSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK
>Mature_311_residues
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAGEDPTPALVGADVVLISAGVA
RKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNPVNTTVAIAAEVMKKAGVYDKNRLFGVTTLDVIRSETFIAE
LKGLNVADVKINVIGGHSGVTILPLLSQVEGVTFSDEEVASLTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGMSLVR
GLQGEANVVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLKGDIKLGVDFVK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=56.0897435897436, Blast_Score=325, Evalue=2e-89,
Organism=Homo sapiens, GI47059044, Length=290, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI221136809, Length=290, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789632, Length=310, Percent_Identity=80, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=57.5079872204473, Blast_Score=327, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=50.7936507936508, Blast_Score=282, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6320125, Length=345, Percent_Identity=36.8115942028986, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324446, Length=367, Percent_Identity=36.2397820163488, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=55.4838709677419, Blast_Score=320, Evalue=7e-88,
Organism=Drosophila melanogaster, GI24663599, Length=313, Percent_Identity=47.6038338658147, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44.6666666666667, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136226, Length=326, Percent_Identity=23.9263803680982, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32110; Mature: 32110

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGVTTLDVIRSETFIAELKGLNVADVKINVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVTFSDEEVASLTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGMSLVR
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCCEECEEECC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPAGSKLSLYDIAPVTPGVAVDLSHIPTAVEIKGFAG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDPTPALVGADVVLISAGVARKPGMDRSDLFNINAGIVRNLIEKVAATCPKALVGIITNP
CCCCCHHHCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCHHHHHHHHCCC
VNTTVAIAAEVMKKAGVYDKNRLFGVTTLDVIRSETFIAELKGLNVADVKINVIGGHSGV
CCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCC
TILPLLSQVEGVTFSDEEVASLTKRIQNAGTEVVEAKAGGGSATLSMGQAACRFGMSLVR
CHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
GLQGEANVVECAYVDGGSEHAEFFAQPVLLGKNGIEKVLPYGEVSAFEANARDSMLDTLK
CCCCCCCEEEEEEECCCCHHHHHHHCCCEECCCCHHHHCCCCCCEEECCCCHHHHHHHHC
GDIKLGVDFVK
CCCEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA