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The map label for this gene is clpB-2 [H]

Identifier: 119946869

GI number: 119946869

Start: 4027274

End: 4029847

Strand: Reverse

Name: clpB-2 [H]

Synonym: Ping_3263

Alternate gene names: 119946869

Gene position: 4029847-4027274 (Counterclockwise)

Preceding gene: 119946870

Following gene: 119946867

Centisome position: 88.38

GC content: 43.05

Gene sequence:

>2574_bases
ATGCGCATGGATAAATTAACCAGTAAGTTTCAATTAGCAGTTGCCGATGCTCAATCCTTAGCACTCGGGCATGATAACCA
ATATATTGAACCCATTCATATTATGCTTACTCTCCTAAATCAAGATGGTGGTTCACTGCGGCCAATATTTGCAGATTTGG
AGGTGAACCTGAGTGCCTTACGATTAGAACTTTCCACTGAGCTGGATCGCCTTCCAAAAGTAACCGGTATCGGCGGTGAT
ATTCAGATATCCGCTCAACTTAATACGTTGTTTAATCTGTGCGACAAATATGTCCAAAAATCCCAGGATAAATTTATTTC
TTCAGAACTTTTTTTAGTCGCGGCTTGTGAAGATAAGGGGCGTTTAGGTGACATTTTAAAAAAATTCGGTTTGAATAAAC
AAAAAGTTGAAAAATCGATTGAAAAAATTCGCAATGGCCAAAATGTCACGGATCAGGATGCTGAGGGGGTAGAGAAAGCA
TTAGTTAAATATACGATTGATTTAACCGAGCAGGCTGAGCAGGGCAAGTTGGATCCGGTCATTGGTCGCGATGATGAAGT
ACGTCGTATGATTCAGGTGCTGCAACGCCGGACTAAAAATAATCCGGTTATTATCGGTGAGCCGGGTGTGGGTAAAACCG
CTATTGTCGAAGGTCTGGCCCAGCGTATTATTAATGGCGAAGTGCCGGAAGGTTTAAAAAATAAACGGGTATTGTCACTG
GATATGGGGGCATTAATTGCCGGCGCAAAATACCGCGGTGAATTTGAAGAAAGATTAAAAGCGGTATTGAGTGAGTTATC
TAAAGAAGAGGGAAAAATCATTCTCTTTATTGATGAATTACATACTATGGTGGGTGCGGGCAAAGCGGATGGTGCGATGG
ACGCGGGTAATATGTTAAAACCGGCCCTGGCGCGGGGAGATCTGCACTGTGTAGGGGCAACTACTCTGGATGAATACCGT
AAATATATTGAAAAAGATGCCGCCCTTGAACGCCGTTTCCAAAAAGTGCTGGTCGATGAACCAACGGTTGAAGATACTAT
TGCCATTTTACGTGGTTTAAAAGAGCGTTATGAACTCCATCATAAGGTCGAAATTACCGATCCTGCGATCGTAGCGGCAG
CGACACTTTCCCATCGTTATATTGCCGATCGCCAGCTCCCCGATAAAGCGATTGACCTGATTGATGAAGCGGCATCAAGC
ATCCGTCTGCAGATGGACTCTAAGCCAGAAGAGTTAGATAAGTTAGAGCGGAAAATCATCCAGTTAAAGTTAGAAGAAAA
GGCGCTGCAAAACGATACTGATGAAGCCAGTATTAAACGTCTAGCTGATTTAGAAAGCGCTCTAGCGGAAAAAGAACAGG
CATTTTTGGCCTTAGATAAAGTATGGCGCGCCGAAAAAGCCGCTCTGTCAGGTACGCAGAATATTAAACAATCCATCGAA
CAGGCACGTTTAGATCTTGAAGTTGCCAGCCGGGCAGTCGATTTAACCCGAATGTCTGAATTGCAGTACGGTGTAATCCC
TGCATTGGAAAAACAGTTAGTACAAGCAGCACAAGCTGAAATGCAGGAGACTACCCTATTAAAACATGCGGTAACCGAAA
ATGAAATTGCTGAAATCCTTGCTCATTGGACGGGTATTCCGGTGGCTAAAATGCTTGAAGGGGAACGCGATAAACTCCTT
AAAATGGAAACGCATCTGCATTCCCGGGTGATAGGCCAAAACGAAGCCATTGACGCTATCGCTAATGCTATTCGCCGCAG
CCGAGCGGGGTTAAGCGATCCCAATCGTCCGATCGGCTCTTTCCTCTTTTTAGGGCCAACGGGGGTGGGTAAAACTGAAT
CCTGTAAAGCATTAGCTAATTTCCTCTTTGACAGTGAGGACAATATGGTGCGCATTGATATGTCTGAGTTTATGGAAAAA
TTTTCAGTGTCCCGTTTAGTGGGGGCACCTCCCGGATATGTAGGATATGAAGAGGGTGGATATTTAACGGAAGCTGTGCG
TCGTAAACCTTATTCAGTGATTTTATTAGATGAGATAGAAAAAGCCCACCCAGACGTGTTTAATATTTTATTACAGGTAT
TAGATGACGGACGTTTAACCGATGGACAAGGGCGTACCGTTAGTTTCCGTAATACCGTTATTATCATGACCTCAAATATC
GGTTCAGATATTATTCAGGAGAAGTTTGATAAAGTCAGCTATGATGAAATTAAATCTTATCTGCTGGATATATTAGCGAG
CCAATTTAAACCCGAATTTTTAAATAGAATAGATGAAACCGTGGTTTTCCATCCATTAGGCAGGGAGCACATTAAATCGA
TTGCAGAGATTCAATTACGATCTTTACAAAAACGTCTTACTGATAAAGATTATCTGGTCACCTTTACTGACCAAGCTATC
CAATTGTTAGTGGACGCGGGTTATGATCCCGTTTATGGCGCCAGACCTTTAAAACGTGCCATTCAGCAATTGCTGGAAAA
CTCCTTAGCGCAGGATATCCTGTCTGGACGTTTGCAAACGGGTAAAGAGATACGGGCGGAGGTTATTGATGGAAAAATTG
AATTTGTGCAATAA

Upstream 100 bases:

>100_bases
ATCCTTTTCATCAAAAATTGCAGCTGTTTTATCTTTCCTCCTTGAAATTGTTCAATTAACCACCATTCTTTATGTCTATA
TTATTATTGAGGGTTTTCCT

Downstream 100 bases:

>100_bases
ATAGCGCAGGCAGATGCAGCTTGAAGTACATAGAAAACCACCGCCAATTTAATTAGCGGTGGTTTTCTGGTTTATGCGTT
TAGTTTAAAAAGGCACAGGG

Product: chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRMDKLTSKFQLAVADAQSLALGHDNQYIEPIHIMLTLLNQDGGSLRPIFADLEVNLSALRLELSTELDRLPKVTGIGGD
IQISAQLNTLFNLCDKYVQKSQDKFISSELFLVAACEDKGRLGDILKKFGLNKQKVEKSIEKIRNGQNVTDQDAEGVEKA
LVKYTIDLTEQAEQGKLDPVIGRDDEVRRMIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLSELSKEEGKIILFIDELHTMVGAGKADGAMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHKVEITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRLQMDSKPEELDKLERKIIQLKLEEKALQNDTDEASIKRLADLESALAEKEQAFLALDKVWRAEKAALSGTQNIKQSIE
QARLDLEVASRAVDLTRMSELQYGVIPALEKQLVQAAQAEMQETTLLKHAVTENEIAEILAHWTGIPVAKMLEGERDKLL
KMETHLHSRVIGQNEAIDAIANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTESCKALANFLFDSEDNMVRIDMSEFMEK
FSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDGQGRTVSFRNTVIIMTSNI
GSDIIQEKFDKVSYDEIKSYLLDILASQFKPEFLNRIDETVVFHPLGREHIKSIAEIQLRSLQKRLTDKDYLVTFTDQAI
QLLVDAGYDPVYGARPLKRAIQQLLENSLAQDILSGRLQTGKEIRAEVIDGKIEFVQ

Sequences:

>Translated_857_residues
MRMDKLTSKFQLAVADAQSLALGHDNQYIEPIHIMLTLLNQDGGSLRPIFADLEVNLSALRLELSTELDRLPKVTGIGGD
IQISAQLNTLFNLCDKYVQKSQDKFISSELFLVAACEDKGRLGDILKKFGLNKQKVEKSIEKIRNGQNVTDQDAEGVEKA
LVKYTIDLTEQAEQGKLDPVIGRDDEVRRMIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLSELSKEEGKIILFIDELHTMVGAGKADGAMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHKVEITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRLQMDSKPEELDKLERKIIQLKLEEKALQNDTDEASIKRLADLESALAEKEQAFLALDKVWRAEKAALSGTQNIKQSIE
QARLDLEVASRAVDLTRMSELQYGVIPALEKQLVQAAQAEMQETTLLKHAVTENEIAEILAHWTGIPVAKMLEGERDKLL
KMETHLHSRVIGQNEAIDAIANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTESCKALANFLFDSEDNMVRIDMSEFMEK
FSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDGQGRTVSFRNTVIIMTSNI
GSDIIQEKFDKVSYDEIKSYLLDILASQFKPEFLNRIDETVVFHPLGREHIKSIAEIQLRSLQKRLTDKDYLVTFTDQAI
QLLVDAGYDPVYGARPLKRAIQQLLENSLAQDILSGRLQTGKEIRAEVIDGKIEFVQ
>Mature_857_residues
MRMDKLTSKFQLAVADAQSLALGHDNQYIEPIHIMLTLLNQDGGSLRPIFADLEVNLSALRLELSTELDRLPKVTGIGGD
IQISAQLNTLFNLCDKYVQKSQDKFISSELFLVAACEDKGRLGDILKKFGLNKQKVEKSIEKIRNGQNVTDQDAEGVEKA
LVKYTIDLTEQAEQGKLDPVIGRDDEVRRMIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLSELSKEEGKIILFIDELHTMVGAGKADGAMDAGNMLKPALARGDLHCVGATTLDEYR
KYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHKVEITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASS
IRLQMDSKPEELDKLERKIIQLKLEEKALQNDTDEASIKRLADLESALAEKEQAFLALDKVWRAEKAALSGTQNIKQSIE
QARLDLEVASRAVDLTRMSELQYGVIPALEKQLVQAAQAEMQETTLLKHAVTENEIAEILAHWTGIPVAKMLEGERDKLL
KMETHLHSRVIGQNEAIDAIANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKTESCKALANFLFDSEDNMVRIDMSEFMEK
FSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDGQGRTVSFRNTVIIMTSNI
GSDIIQEKFDKVSYDEIKSYLLDILASQFKPEFLNRIDETVVFHPLGREHIKSIAEIQLRSLQKRLTDKDYLVTFTDQAI
QLLVDAGYDPVYGARPLKRAIQQLLENSLAQDILSGRLQTGKEIRAEVIDGKIEFVQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=311, Percent_Identity=36.9774919614148, Blast_Score=202, Evalue=9e-52,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=71.9953325554259, Blast_Score=1278, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=484, Percent_Identity=36.3636363636364, Blast_Score=278, Evalue=7e-76,
Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=53.0898876404494, Blast_Score=758, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=41.1695906432749, Blast_Score=636, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95647; Mature: 95647

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMDKLTSKFQLAVADAQSLALGHDNQYIEPIHIMLTLLNQDGGSLRPIFADLEVNLSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
RLELSTELDRLPKVTGIGGDIQISAQLNTLFNLCDKYVQKSQDKFISSELFLVAACEDKG
HHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
RLGDILKKFGLNKQKVEKSIEKIRNGQNVTDQDAEGVEKALVKYTIDLTEQAEQGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEVRRMIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKAVLSELSKEEGKIILFIDELHTMVGAGKADGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HKVEITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRLQMDSKPEELDKLERKII
HEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
QLKLEEKALQNDTDEASIKRLADLESALAEKEQAFLALDKVWRAEKAALSGTQNIKQSIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARLDLEVASRAVDLTRMSELQYGVIPALEKQLVQAAQAEMQETTLLKHAVTENEIAEIL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHWTGIPVAKMLEGERDKLLKMETHLHSRVIGQNEAIDAIANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTESCKALANFLFDSEDNMVRIDMSEFMEKFSVSRLVGAPPGYVGYEEGG
EEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDGQGRTVSFRNTVIIMTSNI
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDIIQEKFDKVSYDEIKSYLLDILASQFKPEFLNRIDETVVFHPLGREHIKSIAEIQLR
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
SLQKRLTDKDYLVTFTDQAIQLLVDAGYDPVYGARPLKRAIQQLLENSLAQDILSGRLQT
HHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GKEIRAEVIDGKIEFVQ
CHHHHHHHHCCEEEECC
>Mature Secondary Structure
MRMDKLTSKFQLAVADAQSLALGHDNQYIEPIHIMLTLLNQDGGSLRPIFADLEVNLSAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
RLELSTELDRLPKVTGIGGDIQISAQLNTLFNLCDKYVQKSQDKFISSELFLVAACEDKG
HHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
RLGDILKKFGLNKQKVEKSIEKIRNGQNVTDQDAEGVEKALVKYTIDLTEQAEQGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEVRRMIQVLQRRTKNNPVIIGEPGVGKTAIVEGLAQRIINGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKAVLSELSKEEGKIILFIDELHTMVGAGKADGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGDLHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HKVEITDPAIVAAATLSHRYIADRQLPDKAIDLIDEAASSIRLQMDSKPEELDKLERKII
HEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHH
QLKLEEKALQNDTDEASIKRLADLESALAEKEQAFLALDKVWRAEKAALSGTQNIKQSIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QARLDLEVASRAVDLTRMSELQYGVIPALEKQLVQAAQAEMQETTLLKHAVTENEIAEIL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHWTGIPVAKMLEGERDKLLKMETHLHSRVIGQNEAIDAIANAIRRSRAGLSDPNRPIGS
HHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTESCKALANFLFDSEDNMVRIDMSEFMEKFSVSRLVGAPPGYVGYEEGG
EEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDGQGRTVSFRNTVIIMTSNI
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDIIQEKFDKVSYDEIKSYLLDILASQFKPEFLNRIDETVVFHPLGREHIKSIAEIQLR
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
SLQKRLTDKDYLVTFTDQAIQLLVDAGYDPVYGARPLKRAIQQLLENSLAQDILSGRLQT
HHHHHCCCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GKEIRAEVIDGKIEFVQ
CHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]