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The map label for this gene is murD [H]

Identifier: 119944901

GI number: 119944901

Start: 1433668

End: 1435005

Strand: Direct

Name: murD [H]

Synonym: Ping_1145

Alternate gene names: 119944901

Gene position: 1433668-1435005 (Clockwise)

Preceding gene: 119944900

Following gene: 119944902

Centisome position: 31.44

GC content: 40.88

Gene sequence:

>1338_bases
ATGTCAAACCAACAGATTGCAGTACTAGGGCTTGGAGAAACCGGATTATCTTGTGTTAATTTTTTATTAAAACAAGGTGA
GCAAGTGACTGTATTTGATACGCGGCAAAGCCCTCCCGGTGAGGATAAGCTTGATAGTTCAGTTAAACTTATCAAAGGTG
AATTAAGCTTTGAGTTGCTCGCCAAGTTTAATTTAATTATTGCAAGCCCGGGCATCGCGTTGGCGACGCCGGCATTACAA
TTTGCTCTGGCCAATGGTTGTGAGATTATTGGTGATATAGAGTTATTTGCTAGAGAGCTTTCAAAACCTGCTTATGCACA
TGCTAAATTAGTTACTATCAGCGGTTCAAATGGTAAAAGTACAGTGACCAGTTTACTTGGCGAAATGGCGGTCGCTGCAA
AAATAAAAGTGGCTGTTGGTGGTAATATTGGCATTCCCGCCCTTGATTTACTTTCTCCTGAAATTGATTTGTATATCTTA
GAGTTATCGAGTTTTCAATTAGAAACTACCTTTTCATTAAATGCGGATATCGCAACGTTATTAAATATTAGTGAAGATCA
TATGGACAGATATGACTCCTATGCGCAGTATACGGCAGCGAAACATCGTCTCTATCAGCAATGTAAAATGGCACTGTATA
ATCGCGATGATAAGCTCACTTACCCAAAAAATCATAGTCTTAATAAAACCAGCTTTGGTTTTGATAAGCTTGCTTATGGA
TTGGTAACCGATGCAGATAAGCAGATTTTTTTGGCAAAAAATGGTTCCCCAATACTCGCTGCTAAACAATTGAAATTGAG
TGGTAAGCATAATTGGATGAATACGTTGGCCGCTTTTGCATTGGGTGAGGCTGTCGGTTTAAGCGAAATCGGAATGTTTA
CAGCCTTACAAGCATATTCTGGTTTGCCGCACCGTTGTGAATTTGTTGCTGAGATAAACAATGTTCGTTGGATAAACGAT
TCAAAAGCCACCAATATAGGTGCCGCACAGGCTGCATTATTGGGTTTGTCCGAAACCATACCGGGTAATGTGCATGTTAT
TTTAGGCGGTGATGGCAAAGGTGCCGATTTTAGTGAGTTAGCGCTGGTGCTGAAAGAGATCCAAGGGATGATCGTCTGTT
TTGGAGTTGACGGAAAAAAAATAGCGGCACAACATAAACGCGCACTGCTGGTTGAAAATTTAGAGCAGGCCGTTGAGAAC
ATTTTCACAACGGCACAAGCAGGGGACTTGGTCATATTAAGCCCTGCTTGTGCCAGTTTAGATATGTATAAAAACTTTAT
GCAACGAGGAGATCACTTTAAACGCTTAGTCAAGGCATTGCCAGCGAGAAAAAACTGA

Upstream 100 bases:

>100_bases
GAGAAAAAAGGTTGGCCTGAGCCGCGTGTTATTGTCCGTTTTTGGATTATTACCATAGTGTTAGTATTAATAGGTTTAGT
GACCTTAAAGGTACGTTAAA

Downstream 100 bases:

>100_bases
TGGCATTATTTAATCTTTTTATACAAAGGAGTACCAACAATTCCTCTGAGCCCTATGACAGGCAACTTTTAATTGCTACG
TTTATTCTTATGTGTATTGG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSNQQIAVLGLGETGLSCVNFLLKQGEQVTVFDTRQSPPGEDKLDSSVKLIKGELSFELLAKFNLIIASPGIALATPALQ
FALANGCEIIGDIELFARELSKPAYAHAKLVTISGSNGKSTVTSLLGEMAVAAKIKVAVGGNIGIPALDLLSPEIDLYIL
ELSSFQLETTFSLNADIATLLNISEDHMDRYDSYAQYTAAKHRLYQQCKMALYNRDDKLTYPKNHSLNKTSFGFDKLAYG
LVTDADKQIFLAKNGSPILAAKQLKLSGKHNWMNTLAAFALGEAVGLSEIGMFTALQAYSGLPHRCEFVAEINNVRWIND
SKATNIGAAQAALLGLSETIPGNVHVILGGDGKGADFSELALVLKEIQGMIVCFGVDGKKIAAQHKRALLVENLEQAVEN
IFTTAQAGDLVILSPACASLDMYKNFMQRGDHFKRLVKALPARKN

Sequences:

>Translated_445_residues
MSNQQIAVLGLGETGLSCVNFLLKQGEQVTVFDTRQSPPGEDKLDSSVKLIKGELSFELLAKFNLIIASPGIALATPALQ
FALANGCEIIGDIELFARELSKPAYAHAKLVTISGSNGKSTVTSLLGEMAVAAKIKVAVGGNIGIPALDLLSPEIDLYIL
ELSSFQLETTFSLNADIATLLNISEDHMDRYDSYAQYTAAKHRLYQQCKMALYNRDDKLTYPKNHSLNKTSFGFDKLAYG
LVTDADKQIFLAKNGSPILAAKQLKLSGKHNWMNTLAAFALGEAVGLSEIGMFTALQAYSGLPHRCEFVAEINNVRWIND
SKATNIGAAQAALLGLSETIPGNVHVILGGDGKGADFSELALVLKEIQGMIVCFGVDGKKIAAQHKRALLVENLEQAVEN
IFTTAQAGDLVILSPACASLDMYKNFMQRGDHFKRLVKALPARKN
>Mature_444_residues
SNQQIAVLGLGETGLSCVNFLLKQGEQVTVFDTRQSPPGEDKLDSSVKLIKGELSFELLAKFNLIIASPGIALATPALQF
ALANGCEIIGDIELFARELSKPAYAHAKLVTISGSNGKSTVTSLLGEMAVAAKIKVAVGGNIGIPALDLLSPEIDLYILE
LSSFQLETTFSLNADIATLLNISEDHMDRYDSYAQYTAAKHRLYQQCKMALYNRDDKLTYPKNHSLNKTSFGFDKLAYGL
VTDADKQIFLAKNGSPILAAKQLKLSGKHNWMNTLAAFALGEAVGLSEIGMFTALQAYSGLPHRCEFVAEINNVRWINDS
KATNIGAAQAALLGLSETIPGNVHVILGGDGKGADFSELALVLKEIQGMIVCFGVDGKKIAAQHKRALLVENLEQAVENI
FTTAQAGDLVILSPACASLDMYKNFMQRGDHFKRLVKALPARKN

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=440, Percent_Identity=51.8181818181818, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48009; Mature: 47878

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQQIAVLGLGETGLSCVNFLLKQGEQVTVFDTRQSPPGEDKLDSSVKLIKGELSFELL
CCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCCCHHEEECCCCCEEH
AKFNLIIASPGIALATPALQFALANGCEIIGDIELFARELSKPAYAHAKLVTISGSNGKS
HEEEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCEEEEEEEECCCCHH
TVTSLLGEMAVAAKIKVAVGGNIGIPALDLLSPEIDLYILELSSFQLETTFSLNADIATL
HHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCCCCEEEEEEECCEEEEEEEECCCCHHHH
LNISEDHMDRYDSYAQYTAAKHRLYQQCKMALYNRDDKLTYPKNHSLNKTSFGFDKLAYG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHH
LVTDADKQIFLAKNGSPILAAKQLKLSGKHNWMNTLAAFALGEAVGLSEIGMFTALQAYS
HEECCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLPHRCEFVAEINNVRWINDSKATNIGAAQAALLGLSETIPGNVHVILGGDGKGADFSEL
CCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
ALVLKEIQGMIVCFGVDGKKIAAQHKRALLVENLEQAVENIFTTAQAGDLVILSPACASL
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
DMYKNFMQRGDHFKRLVKALPARKN
HHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNQQIAVLGLGETGLSCVNFLLKQGEQVTVFDTRQSPPGEDKLDSSVKLIKGELSFELL
CCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCCCHHEEECCCCCEEH
AKFNLIIASPGIALATPALQFALANGCEIIGDIELFARELSKPAYAHAKLVTISGSNGKS
HEEEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCEEEEEEEECCCCHH
TVTSLLGEMAVAAKIKVAVGGNIGIPALDLLSPEIDLYILELSSFQLETTFSLNADIATL
HHHHHHHHHHHHEEEEEEECCCCCCCHHHHCCCCCEEEEEEECCEEEEEEEECCCCHHHH
LNISEDHMDRYDSYAQYTAAKHRLYQQCKMALYNRDDKLTYPKNHSLNKTSFGFDKLAYG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHH
LVTDADKQIFLAKNGSPILAAKQLKLSGKHNWMNTLAAFALGEAVGLSEIGMFTALQAYS
HEECCCCEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GLPHRCEFVAEINNVRWINDSKATNIGAAQAALLGLSETIPGNVHVILGGDGKGADFSEL
CCCHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
ALVLKEIQGMIVCFGVDGKKIAAQHKRALLVENLEQAVENIFTTAQAGDLVILSPACASL
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHH
DMYKNFMQRGDHFKRLVKALPARKN
HHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA