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The map label for this gene is purU [H]

Identifier: 119944785

GI number: 119944785

Start: 1296041

End: 1296931

Strand: Direct

Name: purU [H]

Synonym: Ping_1027

Alternate gene names: 119944785

Gene position: 1296041-1296931 (Clockwise)

Preceding gene: 119944784

Following gene: 119944786

Centisome position: 28.42

GC content: 43.66

Gene sequence:

>891_bases
ATGTCACTTTCAACATCTGTGCAGGAACAAGTTTCAGATACACAGATTATCACCGCAAGCTGTCCCGGCGGTCTGGGGAC
GGTCGATGCTGTCACTGCTTACCTGTATCAACAGGGCTGTTATATTAATGAAATCAACTCATTTGATGATATCGATACGC
AACGTTTTTTTATCCGCATAGAATTCAGGCCGGATCGGCAAAGCGCATTTAACCGCGAAAATTTTATCAGTGGCTTTAAA
ACTAAGGCAGCAAGCTTTGATATGGATTGGACGCTGACCAGAAAAGACGATAAGGCAAAAGTGGTGATCATGGTTTCCAA
GCATGATCATTGTTTAAATGATTTACTCTACCGTTATCGCACGGGCGATTTAAAAATAGAAATCCCCGCTATTATCTCCA
ACCATCCGGACCTTGAAGAACTGGCTAAATGGCATGGTATTCCCTATTTTCATCTACCGGTTAATAAAGACATAAAACCA
CAACAGGAAGCGATGATCTGGAAAATCATTCAGGACTGTGATGCCGATTTAGTGGTGCTTGCCCGTTATATGCAGGTCTT
ATCCAGTGAGATGTGTCAACGACTGGCGGGTTGGGCGATTAATATTCATCACTCACTACTGCCGGGATTCAAAGGTGCTA
AACCCTATTATCAGGCCTATCATAAAGGGGTTAAATTAGTCGGAGCCACAGCCCATTATATCAGTGATGATCTGGATGAA
GGGCCTATTATCACTCAAGGGGTTGAAACTGTTGACCACAGTCACTATCCAGCGGATTTAGCGGCTAAGGGGCAAGCTAT
TGAATGCCAGACATTATCACGGGCGGTGCGCTGGCATATTGAACAGCGTGTTTTTTTACATGGTGAAAAGTCGGTAGTTT
TCGCAAAATAA

Upstream 100 bases:

>100_bases
CGAGCCAACTTTTTTAAGCTGTTAAATCTGTTATAAAGTGCTACTCAACTAAACTCTGACTAAAAAATACTTTTAGTCTC
TGCGAATACTTGGAGTCGCC

Downstream 100 bases:

>100_bases
ATGCTGAATAAATTTTCAGTCATCTTTCGAGTGCCTGCTTATTTGCTCTTTGGCTTTATACTAAGTGTGTTCAGATGTGA
TCTTGCATTGCGGAGGAAAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSLSTSVQEQVSDTQIITASCPGGLGTVDAVTAYLYQQGCYINEINSFDDIDTQRFFIRIEFRPDRQSAFNRENFISGFK
TKAASFDMDWTLTRKDDKAKVVIMVSKHDHCLNDLLYRYRTGDLKIEIPAIISNHPDLEELAKWHGIPYFHLPVNKDIKP
QQEAMIWKIIQDCDADLVVLARYMQVLSSEMCQRLAGWAINIHHSLLPGFKGAKPYYQAYHKGVKLVGATAHYISDDLDE
GPIITQGVETVDHSHYPADLAAKGQAIECQTLSRAVRWHIEQRVFLHGEKSVVFAK

Sequences:

>Translated_296_residues
MSLSTSVQEQVSDTQIITASCPGGLGTVDAVTAYLYQQGCYINEINSFDDIDTQRFFIRIEFRPDRQSAFNRENFISGFK
TKAASFDMDWTLTRKDDKAKVVIMVSKHDHCLNDLLYRYRTGDLKIEIPAIISNHPDLEELAKWHGIPYFHLPVNKDIKP
QQEAMIWKIIQDCDADLVVLARYMQVLSSEMCQRLAGWAINIHHSLLPGFKGAKPYYQAYHKGVKLVGATAHYISDDLDE
GPIITQGVETVDHSHYPADLAAKGQAIECQTLSRAVRWHIEQRVFLHGEKSVVFAK
>Mature_295_residues
SLSTSVQEQVSDTQIITASCPGGLGTVDAVTAYLYQQGCYINEINSFDDIDTQRFFIRIEFRPDRQSAFNRENFISGFKT
KAASFDMDWTLTRKDDKAKVVIMVSKHDHCLNDLLYRYRTGDLKIEIPAIISNHPDLEELAKWHGIPYFHLPVNKDIKPQ
QEAMIWKIIQDCDADLVVLARYMQVLSSEMCQRLAGWAINIHHSLLPGFKGAKPYYQAYHKGVKLVGATAHYISDDLDEG
PIITQGVETVDHSHYPADLAAKGQAIECQTLSRAVRWHIEQRVFLHGEKSVVFAK

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787483, Length=287, Percent_Identity=36.9337979094077, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=29.0155440414508, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=159, Percent_Identity=32.7044025157233, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33618; Mature: 33486

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSTSVQEQVSDTQIITASCPGGLGTVDAVTAYLYQQGCYINEINSFDDIDTQRFFIRI
CCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEE
EFRPDRQSAFNRENFISGFKTKAASFDMDWTLTRKDDKAKVVIMVSKHDHCLNDLLYRYR
EECCCHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHC
TGDLKIEIPAIISNHPDLEELAKWHGIPYFHLPVNKDIKPQQEAMIWKIIQDCDADLVVL
CCCEEEEECHHCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHH
ARYMQVLSSEMCQRLAGWAINIHHSLLPGFKGAKPYYQAYHKGVKLVGATAHYISDDLDE
HHHHHHHHHHHHHHHHCCEEEEHHHHCCCCCCCCHHHHHHHCCEEEEEHHHHHHCCCCCC
GPIITQGVETVDHSHYPADLAAKGQAIECQTLSRAVRWHIEQRVFLHGEKSVVFAK
CCEEECCHHHHCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHHEEEEECCCEEEEEC
>Mature Secondary Structure 
SLSTSVQEQVSDTQIITASCPGGLGTVDAVTAYLYQQGCYINEINSFDDIDTQRFFIRI
CCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEE
EFRPDRQSAFNRENFISGFKTKAASFDMDWTLTRKDDKAKVVIMVSKHDHCLNDLLYRYR
EECCCHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHC
TGDLKIEIPAIISNHPDLEELAKWHGIPYFHLPVNKDIKPQQEAMIWKIIQDCDADLVVL
CCCEEEEECHHCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHH
ARYMQVLSSEMCQRLAGWAINIHHSLLPGFKGAKPYYQAYHKGVKLVGATAHYISDDLDE
HHHHHHHHHHHHHHHHCCEEEEHHHHCCCCCCCCHHHHHHHCCEEEEEHHHHHHCCCCCC
GPIITQGVETVDHSHYPADLAAKGQAIECQTLSRAVRWHIEQRVFLHGEKSVVFAK
CCEEECCHHHHCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHHEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]