Click here to switch to the map view.

The map label for this gene is greA

Identifier: 119944570

GI number: 119944570

Start: 1011128

End: 1011607

Strand: Direct

Name: greA

Synonym: Ping_0808

Alternate gene names: 119944570

Gene position: 1011128-1011607 (Clockwise)

Preceding gene: 119944569

Following gene: 119944572

Centisome position: 22.18

GC content: 39.38

Gene sequence:

>480_bases
ATGGTTCAATATCCAATGACAGCTAATGGTGCTGCTAAATTACGCAGTGAATTAAAGCAGTTAAAAACAGTCACTCGTCC
TCGTATTGTTAAGGCAATTGCGGAGGCTCGTGAGCACGGCGATTTAAAAGAAAATGCGGAATATCACGCGGCGCGCGAAC
AACAAGGTTTTTGTGAAGGACGTATTCAGGATATTGAAGCTAAATTATCCAATGCACGCATTATTGATATCACTAATATA
ACCAATAATGGCAAGGTTATTTTTGGCGCTACGGTCACTATTGTGAATGTCGAGACCGATGCAGAAGTGACTTATAAAAT
TGTTGGTGATGACGAAGCTAATATAAAAATAAATTTGATTTCTGTTAGTTCTCCGCTTGCTCGTGGTTTGATTGGCAAAA
CGCTAGATGATGAAATCTCCATCGTCACACCGGGTGGTACTATTGATTATGAAATTATCAGCGTTCAATATATTGCCTGA

Upstream 100 bases:

>100_bases
TATTTTCGTTAGACAATGACTAAAGCTGAAAATTATCGTAAAATGGGCGCTAATCCCGAGAGTACAAACACAAACATTGT
GTCAAAAAAGGTAATTTTTA

Downstream 100 bases:

>100_bases
TTTTATAAAAAAGCACAAAAAAGCCCATTAAAAATGGGCTTTTTAATAATGCTTTTGATCATTATTTACGCGGAAGCTCT
ATTTTTTTATCTTCACTTGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MVQYPMTANGAAKLRSELKQLKTVTRPRIVKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQDIEAKLSNARIIDITNI
TNNGKVIFGATVTIVNVETDAEVTYKIVGDDEANIKINLISVSSPLARGLIGKTLDDEISIVTPGGTIDYEIISVQYIA

Sequences:

>Translated_159_residues
MVQYPMTANGAAKLRSELKQLKTVTRPRIVKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQDIEAKLSNARIIDITNI
TNNGKVIFGATVTIVNVETDAEVTYKIVGDDEANIKINLISVSSPLARGLIGKTLDDEISIVTPGGTIDYEIISVQYIA
>Mature_159_residues
MVQYPMTANGAAKLRSELKQLKTVTRPRIVKAIAEAREHGDLKENAEYHAAREQQGFCEGRIQDIEAKLSNARIIDITNI
TNNGKVIFGATVTIVNVETDAEVTYKIVGDDEANIKINLISVSSPLARGLIGKTLDDEISIVTPGGTIDYEIISVQYIA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=68.9873417721519, Blast_Score=233, Evalue=5e-63,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=33.5483870967742, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_PSYIN (A1ST36)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942250.1
- ProteinModelPortal:   A1ST36
- SMR:   A1ST36
- STRING:   A1ST36
- GeneID:   4626381
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0808
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   A1ST36
- BioCyc:   PING357804:PING_0808-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17370; Mature: 17370

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQYPMTANGAAKLRSELKQLKTVTRPRIVKAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
RIQDIEAKLSNARIIDITNITNNGKVIFGATVTIVNVETDAEVTYKIVGDDEANIKINLI
CHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEEEEEECCCEEEEEEECCCCCEEEEEEE
SVSSPLARGLIGKTLDDEISIVTPGGTIDYEIISVQYIA
ECCCCHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MVQYPMTANGAAKLRSELKQLKTVTRPRIVKAIAEAREHGDLKENAEYHAAREQQGFCEG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
RIQDIEAKLSNARIIDITNITNNGKVIFGATVTIVNVETDAEVTYKIVGDDEANIKINLI
CHHHHHHHCCCCEEEEEEEECCCCEEEEEEEEEEEEEECCCEEEEEEECCCCCEEEEEEE
SVSSPLARGLIGKTLDDEISIVTPGGTIDYEIISVQYIA
ECCCCHHHHHCCCCCCCCEEEECCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA