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The map label for this gene is rlmE

Identifier: 119944572

GI number: 119944572

Start: 1012098

End: 1012751

Strand: Direct

Name: rlmE

Synonym: Ping_0810

Alternate gene names: 119944572

Gene position: 1012098-1012751 (Clockwise)

Preceding gene: 119944570

Following gene: 119944573

Centisome position: 22.2

GC content: 42.97

Gene sequence:

>654_bases
ATGGCACAAAAAGACCATCCGAACGGACCTAAAAAAAGTGCGTCGGCGAGCTCCAAGCGTTGGATGTATGAGCATGTAAA
TGATTTTTATGTTAAAGAGGCCACTAAAAAGGGATTACGTTCCCGTGCTGTCTTCAAACTGGAAGAAATCAATAAAAAAG
ACAAATTAATTCGTTCCGGCATGACAGTGGTTGATTTAGGGGCTGCACCAGGAAGCTGGTCACAGTGGGCTGTCGATACC
GTTGGCTTAAAGGGAAAAGTGATTGCCTGTGATATATTGCCCATGGATTCGATTGCAGGCGTTGATTTTTTGCAGGGGGA
TTTTCGAGAAGATGCTGTTGTAAATGCTTTGCTGGCCCGCATTGCAGGTCATAAAGTTGATGTGGTTCTCTCTGATATGT
CGCCTAATATGACCGGCACTGTGGGTATTGACCAGCCTAAATCAATGTACCTTGTTGAGCTTGCCTTAGAAATGTGCAAG
GAAGTATTAGTTAAAGAGGGCAGTTTTGTTGTTAAAGTGTTTATGGGGAGTGAATTTGATCTATTTATGAGTGAAGCGCG
TAAATGCTTTAAATCGGTAAAAACCCGCAAACCTGACTCATCTCGTTCAAGATCACGAGAAGTGTATGTTGTTGCAACGG
GTTATAAGGGGTAA

Upstream 100 bases:

>100_bases
GCATTCTACCCTTAAATATTAACCTTTACTATTTTGATTGTTACTTATACCTGTTTATTGTTTTAAGCACAGGAGATAAC
TAACGTTAAGGAATAAATTG

Downstream 100 bases:

>100_bases
CAATGATGACGGCTTGGATCAGTATTTAGCAACAATTATTAAGGCTTGAATTATAGGGCCACTGTTATTCTTTATTAACA
ATGATTGCGGCTTGGTTTCT

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MAQKDHPNGPKKSASASSKRWMYEHVNDFYVKEATKKGLRSRAVFKLEEINKKDKLIRSGMTVVDLGAAPGSWSQWAVDT
VGLKGKVIACDILPMDSIAGVDFLQGDFREDAVVNALLARIAGHKVDVVLSDMSPNMTGTVGIDQPKSMYLVELALEMCK
EVLVKEGSFVVKVFMGSEFDLFMSEARKCFKSVKTRKPDSSRSRSREVYVVATGYKG

Sequences:

>Translated_217_residues
MAQKDHPNGPKKSASASSKRWMYEHVNDFYVKEATKKGLRSRAVFKLEEINKKDKLIRSGMTVVDLGAAPGSWSQWAVDT
VGLKGKVIACDILPMDSIAGVDFLQGDFREDAVVNALLARIAGHKVDVVLSDMSPNMTGTVGIDQPKSMYLVELALEMCK
EVLVKEGSFVVKVFMGSEFDLFMSEARKCFKSVKTRKPDSSRSRSREVYVVATGYKG
>Mature_216_residues
AQKDHPNGPKKSASASSKRWMYEHVNDFYVKEATKKGLRSRAVFKLEEINKKDKLIRSGMTVVDLGAAPGSWSQWAVDTV
GLKGKVIACDILPMDSIAGVDFLQGDFREDAVVNALLARIAGHKVDVVLSDMSPNMTGTVGIDQPKSMYLVELALEMCKE
VLVKEGSFVVKVFMGSEFDLFMSEARKCFKSVKTRKPDSSRSRSREVYVVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=215, Percent_Identity=33.4883720930233, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.2631578947368, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.2631578947368, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.2631578947368, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI194097365, Length=205, Percent_Identity=30.2439024390244, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=64.8780487804878, Blast_Score=280, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=33.5078534031414, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=220, Percent_Identity=35, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=198, Percent_Identity=32.8282828282828, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=96, Percent_Identity=39.5833333333333, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356387, Length=215, Percent_Identity=33.4883720930233, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=34.0425531914894, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.8282828282828, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=31.6326530612245, Blast_Score=97, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_PSYIN (A1ST38)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942252.1
- ProteinModelPortal:   A1ST38
- SMR:   A1ST38
- STRING:   A1ST38
- GeneID:   4626383
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0810
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1ST38
- BioCyc:   PING357804:PING_0810-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23993; Mature: 23862

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: ACT_SITE 172-172 BINDING 71-71 BINDING 73-73 BINDING 91-91 BINDING 107-107 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKDHPNGPKKSASASSKRWMYEHVNDFYVKEATKKGLRSRAVFKLEEINKKDKLIRSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
MTVVDLGAAPGSWSQWAVDTVGLKGKVIACDILPMDSIAGVDFLQGDFREDAVVNALLAR
CCEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCCHHCCCHHHHCCCHHHHHHHHHHHH
IAGHKVDVVLSDMSPNMTGTVGIDQPKSMYLVELALEMCKEVLVKEGSFVVKVFMGSEFD
HCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
LFMSEARKCFKSVKTRKPDSSRSRSREVYVVATGYKG
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
AQKDHPNGPKKSASASSKRWMYEHVNDFYVKEATKKGLRSRAVFKLEEINKKDKLIRSG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
MTVVDLGAAPGSWSQWAVDTVGLKGKVIACDILPMDSIAGVDFLQGDFREDAVVNALLAR
CCEEEECCCCCCCCCHHHEECCCCCEEEEEEECCCCHHCCCHHHHCCCHHHHHHHHHHHH
IAGHKVDVVLSDMSPNMTGTVGIDQPKSMYLVELALEMCKEVLVKEGSFVVKVFMGSEFD
HCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHH
LFMSEARKCFKSVKTRKPDSSRSRSREVYVVATGYKG
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA