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The map label for this gene is ispF [H]

Identifier: 119944445

GI number: 119944445

Start: 861079

End: 861561

Strand: Direct

Name: ispF [H]

Synonym: Ping_0673

Alternate gene names: 119944445

Gene position: 861079-861561 (Clockwise)

Preceding gene: 119944444

Following gene: 119944446

Centisome position: 18.88

GC content: 43.06

Gene sequence:

>483_bases
ATGATCCGTATCGGACATGGCTTTGATGTGCACAAATTTGGTGGGCAAGGACCTATCACTATTGCCGGCGTGAAAATCCC
TTATCATCAGGGATTAATTGCACATTCCGATGGTGACGTGGCTTTACATGCATTATGTGATGCCCTGTTAGGGGCATTAG
CATTAGGTGATATCGGTCGTCACTTTCCAGACAATGATCCGCAGTATGCAGGGATTGATAGCCGAAAATTATTGGAAAAT
GTTTTTCTGCAGGTTAAAGAGCGCGGTTATCAAATTGGAAATATAGATCTGACTATTGTTGCGGAACAACCTAAGATGGC
CGCGTTTATTCCAGATATGTGCGTCGAAATCTCACAACTCGTCGACACTGAAATCCAAAATGTTAATGTCAAAGCGACGA
CGACGGAAAGATTAGGTGCGATTGGGCGTAGCGAAGGAATCGCTACGCATGCTGTGGTTTTATTAATGAAGAAAAAAATA
TGA

Upstream 100 bases:

>100_bases
GAAGGGCGTAGTGATAATTTAAAAGTGACTCGATCTGAAGATTTAACACTCGCCGAATTATATTTATCATCACCAACAAG
CAAATAATAAAGGGTAACTT

Downstream 100 bases:

>100_bases
CAGAAGAAGAAAAACAAGTATTTAACTTAGATTTTAATTATCTGTATGGCGCGCCACAAAGTGAAGGTATATTTAAACAG
TCATGTGAAGACTTTAACGT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MIRIGHGFDVHKFGGQGPITIAGVKIPYHQGLIAHSDGDVALHALCDALLGALALGDIGRHFPDNDPQYAGIDSRKLLEN
VFLQVKERGYQIGNIDLTIVAEQPKMAAFIPDMCVEISQLVDTEIQNVNVKATTTERLGAIGRSEGIATHAVVLLMKKKI

Sequences:

>Translated_160_residues
MIRIGHGFDVHKFGGQGPITIAGVKIPYHQGLIAHSDGDVALHALCDALLGALALGDIGRHFPDNDPQYAGIDSRKLLEN
VFLQVKERGYQIGNIDLTIVAEQPKMAAFIPDMCVEISQLVDTEIQNVNVKATTTERLGAIGRSEGIATHAVVLLMKKKI
>Mature_160_residues
MIRIGHGFDVHKFGGQGPITIAGVKIPYHQGLIAHSDGDVALHALCDALLGALALGDIGRHFPDNDPQYAGIDSRKLLEN
VFLQVKERGYQIGNIDLTIVAEQPKMAAFIPDMCVEISQLVDTEIQNVNVKATTTERLGAIGRSEGIATHAVVLLMKKKI

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=61.5384615384615, Blast_Score=182, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17276; Mature: 17276

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGHGFDVHKFGGQGPITIAGVKIPYHQGLIAHSDGDVALHALCDALLGALALGDIGR
CEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HFPDNDPQYAGIDSRKLLENVFLQVKERGYQIGNIDLTIVAEQPKMAAFIPDMCVEISQL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
VDTEIQNVNVKATTTERLGAIGRSEGIATHAVVLLMKKKI
HHHHHHCCEEEEECHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRIGHGFDVHKFGGQGPITIAGVKIPYHQGLIAHSDGDVALHALCDALLGALALGDIGR
CEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HFPDNDPQYAGIDSRKLLENVFLQVKERGYQIGNIDLTIVAEQPKMAAFIPDMCVEISQL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCEEEEEEECCCCHHHHHHHHHHHHHHH
VDTEIQNVNVKATTTERLGAIGRSEGIATHAVVLLMKKKI
HHHHHHCCEEEEECHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]