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The map label for this gene is truD [H]

Identifier: 119944446

GI number: 119944446

Start: 861558

End: 862592

Strand: Direct

Name: truD [H]

Synonym: Ping_0674

Alternate gene names: 119944446

Gene position: 861558-862592 (Clockwise)

Preceding gene: 119944445

Following gene: 119944447

Centisome position: 18.9

GC content: 40.29

Gene sequence:

>1035_bases
ATGACAGAAGAAGAAAAACAAGTATTTAACTTAGATTTTAATTATCTGTATGGCGCGCCACAAAGTGAAGGTATATTTAA
ACAGTCATGTGAAGACTTTAACGTTGAGGAAAACCTTGGGTTTGAGTTAACGGGTGAGGGGGAACATGTTTGCTTATGGG
TGCAAAAAGTGGGTGAAAATACGGCCTATCTAGCCAGGGAATTAGCCAAGTTTGCTGCTATTCCAGCTAAAAATGTCAGC
TACGCAGGACTTAAAGATCGAAATGCTGATACTAAACAGTGGTTTTCATTACATATTCCCGGAAAGATAACACCTGATTT
TAGTACCTTTGAATCGCCCGGTGTGACTATCCTTAATGTTGTTCGCCATAATAAAAAGATTAAAATAGGGGCATTAGCGG
GTAATACTTTTGCCATTGTTATACGTGAAATTAGTGATAAAAGTGCAATGGAAAAAGCACTATTGCAGGTTCAAGGGGGT
GTGCCAAATTATTATGGATCACAGCGTTTTGGTTTTGATGGACACAATGTTAATGCAGCATTATTAATGTTCCGGGGCCG
TAAAATTAAAGATCGTTTTAAACGCGGTATGTATTTAAGTGCTGCGCGCAGTTACTTGTTTAATCAGGTTGTGTCAGCAC
GTGTTAAAGATGATTTATATGATAAACCGATCCTCGGAGATTGTGTGCAGTTTGTGGCTAACCGTTCATTTTTCCCGCTA
ACGGAACTTGACAAGCAGACCAAGCAGCGGTTAAAAAGCCGCGAAGTTTGTTTGACAGCCCCTTTGTGGGGAGCGGGCGA
ATTGACTTCATTAGATGATGCAAAACAATATGAAAGTGAACTGTTAACCCCCTATCAGGAGATTCAAGCGGGGCTGGCTA
AAAATGGCTTAAAGCAGGAGCGTCGTCCGCTATTATTAGTACCTGAAAAATTTTCATCAATCTGGCTGGATAATAAAACG
GTCAGAGTTGAGTTTTATTTACCTTCTGGTTGTTACGCAACCAGTGTGTTGCGTGAATTAATAAAGGCAAGCTGA

Upstream 100 bases:

>100_bases
ATGTTAATGTCAAAGCGACGACGACGGAAAGATTAGGTGCGATTGGGCGTAGCGAAGGAATCGCTACGCATGCTGTGGTT
TTATTAATGAAGAAAAAAAT

Downstream 100 bases:

>100_bases
TGACCCTATTAATAAGTAATGATGATGGTGTTTATGCGCCTGGTTTAAACGCGTTATATCATGCATTAAAAGATCTGGCG
GATGTGAAAGTGGTCGCGCC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTEEEKQVFNLDFNYLYGAPQSEGIFKQSCEDFNVEENLGFELTGEGEHVCLWVQKVGENTAYLARELAKFAAIPAKNVS
YAGLKDRNADTKQWFSLHIPGKITPDFSTFESPGVTILNVVRHNKKIKIGALAGNTFAIVIREISDKSAMEKALLQVQGG
VPNYYGSQRFGFDGHNVNAALLMFRGRKIKDRFKRGMYLSAARSYLFNQVVSARVKDDLYDKPILGDCVQFVANRSFFPL
TELDKQTKQRLKSREVCLTAPLWGAGELTSLDDAKQYESELLTPYQEIQAGLAKNGLKQERRPLLLVPEKFSSIWLDNKT
VRVEFYLPSGCYATSVLRELIKAS

Sequences:

>Translated_344_residues
MTEEEKQVFNLDFNYLYGAPQSEGIFKQSCEDFNVEENLGFELTGEGEHVCLWVQKVGENTAYLARELAKFAAIPAKNVS
YAGLKDRNADTKQWFSLHIPGKITPDFSTFESPGVTILNVVRHNKKIKIGALAGNTFAIVIREISDKSAMEKALLQVQGG
VPNYYGSQRFGFDGHNVNAALLMFRGRKIKDRFKRGMYLSAARSYLFNQVVSARVKDDLYDKPILGDCVQFVANRSFFPL
TELDKQTKQRLKSREVCLTAPLWGAGELTSLDDAKQYESELLTPYQEIQAGLAKNGLKQERRPLLLVPEKFSSIWLDNKT
VRVEFYLPSGCYATSVLRELIKAS
>Mature_343_residues
TEEEKQVFNLDFNYLYGAPQSEGIFKQSCEDFNVEENLGFELTGEGEHVCLWVQKVGENTAYLARELAKFAAIPAKNVSY
AGLKDRNADTKQWFSLHIPGKITPDFSTFESPGVTILNVVRHNKKIKIGALAGNTFAIVIREISDKSAMEKALLQVQGGV
PNYYGSQRFGFDGHNVNAALLMFRGRKIKDRFKRGMYLSAARSYLFNQVVSARVKDDLYDKPILGDCVQFVANRSFFPLT
ELDKQTKQRLKSREVCLTAPLWGAGELTSLDDAKQYESELLTPYQEIQAGLAKNGLKQERRPLLLVPEKFSSIWLDNKTV
RVEFYLPSGCYATSVLRELIKAS

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Escherichia coli, GI1789102, Length=336, Percent_Identity=41.3690476190476, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 38809; Mature: 38678

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50984 TRUD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEEKQVFNLDFNYLYGAPQSEGIFKQSCEDFNVEENLGFELTGEGEHVCLWVQKVGEN
CCCCCCEEEECCCCEEECCCCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEHHCCC
TAYLARELAKFAAIPAKNVSYAGLKDRNADTKQWFSLHIPGKITPDFSTFESPGVTILNV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHH
VRHNKKIKIGALAGNTFAIVIREISDKSAMEKALLQVQGGVPNYYGSQRFGFDGHNVNAA
HHCCCEEEEEEEECCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LLMFRGRKIKDRFKRGMYLSAARSYLFNQVVSARVKDDLYDKPILGDCVQFVANRSFFPL
EEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
TELDKQTKQRLKSREVCLTAPLWGAGELTSLDDAKQYESELLTPYQEIQAGLAKNGLKQE
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
RRPLLLVPEKFSSIWLDNKTVRVEFYLPSGCYATSVLRELIKAS
CCCEEEECCHHHHEEECCEEEEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEEEKQVFNLDFNYLYGAPQSEGIFKQSCEDFNVEENLGFELTGEGEHVCLWVQKVGEN
CCCCCEEEECCCCEEECCCCCCCHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEHHCCC
TAYLARELAKFAAIPAKNVSYAGLKDRNADTKQWFSLHIPGKITPDFSTFESPGVTILNV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEHH
VRHNKKIKIGALAGNTFAIVIREISDKSAMEKALLQVQGGVPNYYGSQRFGFDGHNVNAA
HHCCCEEEEEEEECCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
LLMFRGRKIKDRFKRGMYLSAARSYLFNQVVSARVKDDLYDKPILGDCVQFVANRSFFPL
EEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCH
TELDKQTKQRLKSREVCLTAPLWGAGELTSLDDAKQYESELLTPYQEIQAGLAKNGLKQE
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
RRPLLLVPEKFSSIWLDNKTVRVEFYLPSGCYATSVLRELIKAS
CCCEEEECCHHHHEEECCEEEEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA