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The map label for this gene is livK4 [C]

Identifier: 119900019

GI number: 119900019

Start: 4086174

End: 4087289

Strand: Direct

Name: livK4 [C]

Synonym: azo3730

Alternate gene names: 119900019

Gene position: 4086174-4087289 (Clockwise)

Preceding gene: 119900018

Following gene: 119900020

Centisome position: 93.38

GC content: 65.59

Gene sequence:

>1116_bases
ATGAAGAAATCCGTCGTAGCACTTGCCGTGATGGCGCTCGGCATCGGTAGCGCCCAGGCCCAGGAACAGGTCGTCAAGAT
CGGTGGCGTCGCCCCGCTCACCGGCACCATCGCCCACCTCGGCAAGGATCTCGAAAACGGCACCCGCCTCGCGGTGGAAG
AGGCCAACGCCAAGGGCGTCACGATCGGCGGCAAGAAGGTCAAGCTGGAATTCGTCGGCGAGGACGACCAGGCCGACCCG
CGCACCGGTACCACGGTGGCCCAGCGTCTGGTCGACGCGGGCGTGAAGGCTGTCGTCGGCCACCTGAACTCGGGCACCTC
GATTCCGGCGTCGCGCATCTACGATCAGGCCGGCATCGCCCAGGTGACGCCCGCCTCCACCAACCCCAAGCTCACCCAGC
AGGGCTACAAGGGTGTGTTCCGCACCATCGCCAACGACGTGCAGCAGGGCTCGGTGCTCGGCAAGTTCGCCGCCACCAAC
CTGGGCGCGAAGAAGATCGCGATCATCGACGACCGCACCGCCTACGGCCAGGGCCTGGCGGACGAAACCGAAAAGGGCGC
GAAGGCCTCGGGCGCGCAGATCGTGGCGCGTGAATTCACCACCGACAAGGCCACCGACTTCAACGCCATCCTCACCAAGA
TCCGCGCCACCAACCCGGACGTGATCTTCTTCGGCGGCATGGACGCCCAGGCCGGCCCGATGCTGCGCCAGATGAAGCAG
CTCGGCATTTCGGCCAAGTTCCTCACCGGTGACGGCGGCTGCAGCCCCGAGATGATCAAGCTGGCCGGCGACGCGATCAG
CGCCAACGCCTACTGCTCGATGGCCGGCCTGCCGATCGAGAAGATGCCGGGCGGGGCGGACTTCCGCGAGCGCTACAAGA
AGCGCTACAACGCCGACGTGCAGATCTACTCGCCGTATGCCTACGACGCTGCCACCGCGATCATCACCGCGATGCAGAAG
GCGGGCTCGGCTGAGCCGGCCAAGTACATCCCGGAACTGAAGAAGGTGAACTTCCCGGGCGTCATCGGCAACATCTCCTT
CGACGACAAGGGCGACCTGAAGGAAGGCTCGATCACGGTGTACCAGTTCAAGAACGGCGACTGGGCGCCGCTGTAA

Upstream 100 bases:

>100_bases
CTTGCGAGTCGTACCACCCACCCCGCCGCGCTGGAGGTGGCGCCGGAGTGGGAACAACAACACGAAACATCCTTATGTCA
GTTTCGCTGGAGGATCAGGT

Downstream 100 bases:

>100_bases
GCGCCAGCGCAGGGCGCGCGGCCACACGCCGCGCCCCTCGCGGCGCCGGCACCGATGCCGGCGCCGCCTGCTCCACCCGC
ACTTCAAGCTATCCGCCGCG

Product: leucine-specific binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKSVVALAVMALGIGSAQAQEQVVKIGGVAPLTGTIAHLGKDLENGTRLAVEEANAKGVTIGGKKVKLEFVGEDDQADP
RTGTTVAQRLVDAGVKAVVGHLNSGTSIPASRIYDQAGIAQVTPASTNPKLTQQGYKGVFRTIANDVQQGSVLGKFAATN
LGAKKIAIIDDRTAYGQGLADETEKGAKASGAQIVAREFTTDKATDFNAILTKIRATNPDVIFFGGMDAQAGPMLRQMKQ
LGISAKFLTGDGGCSPEMIKLAGDAISANAYCSMAGLPIEKMPGGADFRERYKKRYNADVQIYSPYAYDAATAIITAMQK
AGSAEPAKYIPELKKVNFPGVIGNISFDDKGDLKEGSITVYQFKNGDWAPL

Sequences:

>Translated_371_residues
MKKSVVALAVMALGIGSAQAQEQVVKIGGVAPLTGTIAHLGKDLENGTRLAVEEANAKGVTIGGKKVKLEFVGEDDQADP
RTGTTVAQRLVDAGVKAVVGHLNSGTSIPASRIYDQAGIAQVTPASTNPKLTQQGYKGVFRTIANDVQQGSVLGKFAATN
LGAKKIAIIDDRTAYGQGLADETEKGAKASGAQIVAREFTTDKATDFNAILTKIRATNPDVIFFGGMDAQAGPMLRQMKQ
LGISAKFLTGDGGCSPEMIKLAGDAISANAYCSMAGLPIEKMPGGADFRERYKKRYNADVQIYSPYAYDAATAIITAMQK
AGSAEPAKYIPELKKVNFPGVIGNISFDDKGDLKEGSITVYQFKNGDWAPL
>Mature_371_residues
MKKSVVALAVMALGIGSAQAQEQVVKIGGVAPLTGTIAHLGKDLENGTRLAVEEANAKGVTIGGKKVKLEFVGEDDQADP
RTGTTVAQRLVDAGVKAVVGHLNSGTSIPASRIYDQAGIAQVTPASTNPKLTQQGYKGVFRTIANDVQQGSVLGKFAATN
LGAKKIAIIDDRTAYGQGLADETEKGAKASGAQIVAREFTTDKATDFNAILTKIRATNPDVIFFGGMDAQAGPMLRQMKQ
LGISAKFLTGDGGCSPEMIKLAGDAISANAYCSMAGLPIEKMPGGADFRERYKKRYNADVQIYSPYAYDAATAIITAMQK
AGSAEPAKYIPELKKVNFPGVIGNISFDDKGDLKEGSITVYQFKNGDWAPL

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=33.972602739726, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI48994941, Length=352, Percent_Identity=30.1136363636364, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 38984; Mature: 38984

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVVALAVMALGIGSAQAQEQVVKIGGVAPLTGTIAHLGKDLENGTRLAVEEANAKGV
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCEEEEEECCCCEE
TIGGKKVKLEFVGEDDQADPRTGTTVAQRLVDAGVKAVVGHLNSGTSIPASRIYDQAGIA
EECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
QVTPASTNPKLTQQGYKGVFRTIANDVQQGSVLGKFAATNLGAKKIAIIDDRTAYGQGLA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCHHCCCCC
DETEKGAKASGAQIVAREFTTDKATDFNAILTKIRATNPDVIFFGGMDAQAGPMLRQMKQ
HHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
LGISAKFLTGDGGCSPEMIKLAGDAISANAYCSMAGLPIEKMPGGADFRERYKKRYNADV
HCCEEEEEECCCCCCHHHHEECCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHCCCCE
QIYSPYAYDAATAIITAMQKAGSAEPAKYIPELKKVNFPGVIGNISFDDKGDLKEGSITV
EEECCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCEEE
YQFKNGDWAPL
EEECCCCCCCC
>Mature Secondary Structure
MKKSVVALAVMALGIGSAQAQEQVVKIGGVAPLTGTIAHLGKDLENGTRLAVEEANAKGV
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCHHCCCCEEEEEECCCCEE
TIGGKKVKLEFVGEDDQADPRTGTTVAQRLVDAGVKAVVGHLNSGTSIPASRIYDQAGIA
EECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCC
QVTPASTNPKLTQQGYKGVFRTIANDVQQGSVLGKFAATNLGAKKIAIIDDRTAYGQGLA
EECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCHHCCCCC
DETEKGAKASGAQIVAREFTTDKATDFNAILTKIRATNPDVIFFGGMDAQAGPMLRQMKQ
HHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
LGISAKFLTGDGGCSPEMIKLAGDAISANAYCSMAGLPIEKMPGGADFRERYKKRYNADV
HCCEEEEEECCCCCCHHHHEECCCCCCCCCEEEECCCCHHHCCCCCHHHHHHHHHCCCCE
QIYSPYAYDAATAIITAMQKAGSAEPAKYIPELKKVNFPGVIGNISFDDKGDLKEGSITV
EEECCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHCCCCCEEECCCCCCCCCCCCCCEEE
YQFKNGDWAPL
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA