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The map label for this gene is livK3 [H]

Identifier: 119900018

GI number: 119900018

Start: 4084668

End: 4085792

Strand: Direct

Name: livK3 [H]

Synonym: azo3729

Alternate gene names: 119900018

Gene position: 4084668-4085792 (Clockwise)

Preceding gene: 119900014

Following gene: 119900019

Centisome position: 93.34

GC content: 68.0

Gene sequence:

>1125_bases
ATGAAGACGCCCGTAGCCTGCCTGCTGGCCGCTACCCTCGCCCTGGTGGGCAATTCCGCCGCCCTCGCCCAGACCGTGGT
CAAGATCGGCCACGCCGGCCCGCTCACCGGGCCCATCGCCCATTTCGGCAAGGATGGCGAGATGGGCGCGCGGCTGGCGG
TCGAGGACGCCAACGCCAGGGGCATCACGCTGGGCGGACAGAAGGTGAAGTTCGAGCTGGTCAGCGAGGACGACCAGGGC
GATCCGCGCACTGCCACCACCGTGGCGCAGCGGCTCACCGATCTCGGCGTGAAAGGCGTGGTCGGCCATGTGACCTCGGG
CGCGTCCATCCCGGCGTCGCGGGTGTACGAGCAGGCCGGCGTGCCGGTGGTGACACCCTCCTCCACCAGCCCCAAGCTCA
CCCGCCAGGGCTACAAGGTGACTTTCCGGGTGATCGCCAACGACCTGCAGCAGGGCGAGGCGACGGCGAAGTACGCTGCC
GAGACGCTGAAAGCCGCCAAGGTTGCGATCATCGACGACCGCACCGCCTACGGTCAGGGCCTGGCCGATGCCTTTGCCGA
TGCGGCCAAGCAGCGTGGACTGCAGGTGGTCGGGCGCGAATTCACCACCGACAAATCCACTGACTTCCTCGCCATCCTCA
CCAAGATCAAGGCCAAGGCGCCGGACGCGGTGTTCTATGGCGGCATGGACGCGCAGGCGGCGCCGATGCTGCGCCAGTTG
CGCCAACTGGGCAGCAGTGCCATCTACCTGGGCGGCGACGGCGTATGCACCGGCGAGATGATCAAGCTCGCCGGCGACGC
GATGAGCGACAAGGTGTATTGCTCGCAGGCCAGCCTGCCGCCGGCGAAGATGCCCGGCGGCACGGATTTCCTCAGCCGCT
TCAAGCAGCGCTACAAGGCCGACGTGCTGCTCTATGCGCCCTACAGCTACGACGCCGCCACCGCCCTGATCGAAGCGATG
AAGGCGGCCGACTCCAGCGAGCCGGCCAAGTACCTGCCGGCCCTGCAGCGGGTCGCCTTCAAGGGCGTGACCGGCGAAAT
CGCCTTCGACCCTCAGGGCGACATCCGCAACGGCGGGGTCAGCATGCACCGCTTCCGCGACGGCAAGTGGGTATCGGCCG
ACTGA

Upstream 100 bases:

>100_bases
CCTACCGGCAACGGGCCGCCGGGCGCGGGCTAGAATCTCCGTTGCCGGCCGGAAACGCAATCGCGCCGGGTGACAATCTG
CAACGCACCGGAGAACCGCC

Downstream 100 bases:

>100_bases
GCGCTTCCGCGGCCCGCGCGCGAGCGGGTGACGCGGCTGCACCACCCCGGTGCGCCGCGCCCGCTGTGCGTGCGCCCGGC
ACGCGCGCGCCCTCGGCGGA

Product: leucine-specific binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKTPVACLLAATLALVGNSAALAQTVVKIGHAGPLTGPIAHFGKDGEMGARLAVEDANARGITLGGQKVKFELVSEDDQG
DPRTATTVAQRLTDLGVKGVVGHVTSGASIPASRVYEQAGVPVVTPSSTSPKLTRQGYKVTFRVIANDLQQGEATAKYAA
ETLKAAKVAIIDDRTAYGQGLADAFADAAKQRGLQVVGREFTTDKSTDFLAILTKIKAKAPDAVFYGGMDAQAAPMLRQL
RQLGSSAIYLGGDGVCTGEMIKLAGDAMSDKVYCSQASLPPAKMPGGTDFLSRFKQRYKADVLLYAPYSYDAATALIEAM
KAADSSEPAKYLPALQRVAFKGVTGEIAFDPQGDIRNGGVSMHRFRDGKWVSAD

Sequences:

>Translated_374_residues
MKTPVACLLAATLALVGNSAALAQTVVKIGHAGPLTGPIAHFGKDGEMGARLAVEDANARGITLGGQKVKFELVSEDDQG
DPRTATTVAQRLTDLGVKGVVGHVTSGASIPASRVYEQAGVPVVTPSSTSPKLTRQGYKVTFRVIANDLQQGEATAKYAA
ETLKAAKVAIIDDRTAYGQGLADAFADAAKQRGLQVVGREFTTDKSTDFLAILTKIKAKAPDAVFYGGMDAQAAPMLRQL
RQLGSSAIYLGGDGVCTGEMIKLAGDAMSDKVYCSQASLPPAKMPGGTDFLSRFKQRYKADVLLYAPYSYDAATALIEAM
KAADSSEPAKYLPALQRVAFKGVTGEIAFDPQGDIRNGGVSMHRFRDGKWVSAD
>Mature_374_residues
MKTPVACLLAATLALVGNSAALAQTVVKIGHAGPLTGPIAHFGKDGEMGARLAVEDANARGITLGGQKVKFELVSEDDQG
DPRTATTVAQRLTDLGVKGVVGHVTSGASIPASRVYEQAGVPVVTPSSTSPKLTRQGYKVTFRVIANDLQQGEATAKYAA
ETLKAAKVAIIDDRTAYGQGLADAFADAAKQRGLQVVGREFTTDKSTDFLAILTKIKAKAPDAVFYGGMDAQAAPMLRQL
RQLGSSAIYLGGDGVCTGEMIKLAGDAMSDKVYCSQASLPPAKMPGGTDFLSRFKQRYKADVLLYAPYSYDAATALIEAM
KAADSSEPAKYLPALQRVAFKGVTGEIAFDPQGDIRNGGVSMHRFRDGKWVSAD

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=358, Percent_Identity=32.122905027933, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI48994941, Length=350, Percent_Identity=31.4285714285714, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39281; Mature: 39281

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPVACLLAATLALVGNSAALAQTVVKIGHAGPLTGPIAHFGKDGEMGARLAVEDANAR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCC
GITLGGQKVKFELVSEDDQGDPRTATTVAQRLTDLGVKGVVGHVTSGASIPASRVYEQAG
EEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCC
VPVVTPSSTSPKLTRQGYKVTFRVIANDLQQGEATAKYAAETLKAAKVAIIDDRTAYGQG
CCEECCCCCCCCHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCCHHCCC
LADAFADAAKQRGLQVVGREFTTDKSTDFLAILTKIKAKAPDAVFYGGMDAQAAPMLRQL
HHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHH
RQLGSSAIYLGGDGVCTGEMIKLAGDAMSDKVYCSQASLPPAKMPGGTDFLSRFKQRYKA
HHHCCCEEEECCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCC
DVLLYAPYSYDAATALIEAMKAADSSEPAKYLPALQRVAFKGVTGEIAFDPQGDIRNGGV
CEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCE
SMHRFRDGKWVSAD
EEEEECCCCEECCC
>Mature Secondary Structure
MKTPVACLLAATLALVGNSAALAQTVVKIGHAGPLTGPIAHFGKDGEMGARLAVEDANAR
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCEEEEECCCCC
GITLGGQKVKFELVSEDDQGDPRTATTVAQRLTDLGVKGVVGHVTSGASIPASRVYEQAG
EEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCC
VPVVTPSSTSPKLTRQGYKVTFRVIANDLQQGEATAKYAAETLKAAKVAIIDDRTAYGQG
CCEECCCCCCCCHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCCHHCCC
LADAFADAAKQRGLQVVGREFTTDKSTDFLAILTKIKAKAPDAVFYGGMDAQAAPMLRQL
HHHHHHHHHHHCCHHEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHH
RQLGSSAIYLGGDGVCTGEMIKLAGDAMSDKVYCSQASLPPAKMPGGTDFLSRFKQRYKA
HHHCCCEEEECCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCC
DVLLYAPYSYDAATALIEAMKAADSSEPAKYLPALQRVAFKGVTGEIAFDPQGDIRNGGV
CEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCE
SMHRFRDGKWVSAD
EEEEECCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]