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The map label for this gene is cysB [H]

Identifier: 119774461

GI number: 119774461

Start: 1612876

End: 1613850

Strand: Direct

Name: cysB [H]

Synonym: Sama_1324

Alternate gene names: 119774461

Gene position: 1612876-1613850 (Clockwise)

Preceding gene: 119774459

Following gene: 119774463

Centisome position: 37.46

GC content: 52.51

Gene sequence:

>975_bases
ATGAAACTTCAACAACTCAGATACATTGCCGAAGTGGTTAACCACAACCTGAACGTGTCCGCCACGGCAGAAAACCTCTA
CACGTCCCAGCCGGGGATCAGCAAACAGGTTCGTATGCTCGAAGATGAGCTGGGCATCCAAATTTTTGGACGCAGTGGCA
AGCATTTAACCCACGTAACACCGGCTGGTGAGCAAGTGATTGCCATCGCCAACGATATTCTGGGCAAAGTTGAAAGTATC
AAAAAAGTCGCAGAAGAGTACACCAAGCCGGATCAGGGTGAGTTGAATATTGCCACTACCGATACCCAGGCAAGGTACGC
GCTGCCGCACATCATCAAGGGCTTTATCAAACGCTATCCCAAGGTGAACTTGCACATGCATCAGGGTACGCCAACACAGA
TTAGTGAGTTGGCTGCCCGTGGCGAAGCGGACTTTGCCATCGCCACTGAAGCCATGCATCTTTATTCGGATCTCATCATG
CTCCCTTGCTACCACTGGAATCGCTCCATCGTGGTAACCCGGGATCATCCCCTTGCCGGGCGCAGTCAAATCAGCATCGA
AGAGTTGGCCCGTTATCCACTGGTGACCTACGTGTTCGGTTTTGACCGTGCATCAGAGATTGAACGGGCGTTCAATCGCG
CGGGACTTGAGCCAAGGCTGGTCTTTTCTGCCACCAGTGCCGATGTGCTCAAGACCTATGTTCGCTTGGGACTGGGTGTT
GGGGTGCTGGCGTCCATGGCCATTGACCCCAATATTGACCGGGACCTGGTGGCCATCGATGCCAGCCATCTGTTTGCCCA
TTCCACCACCAAGATTGGCTTTCGAAAGGGCAGTTTCCTCAGAAGCTATATGTATGACTTTATCGAGCTTTTTGCACCTC
ACCTTACCCGTGACGTGGTTGAGAAAGCCGTCGCGCTGCGCGATCCCATACTCATTGAAGAGATGTTTGCCGACAGAGAG
TTACCAATCCGCTGA

Upstream 100 bases:

>100_bases
TCCTGTTTGATTTAATTTAATCGGTGTACTATAACCCATGATTATAGATAGCCAGCTTAAGATTAGCCTCCCACCCCTTG
GGAGTGGCTAAGGAAGTGAG

Downstream 100 bases:

>100_bases
GTTGGCTTGCGCCCATAAAAAATCCCGCACCAAGCGGGATTTTTTATGGAGTGAGAATTAACACTCAACTATGTTAACAG
CCAGACCACCCTTGGCGGTT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIAEVVNHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGEQVIAIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIKGFIKRYPKVNLHMHQGTPTQISELAARGEADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAGRSQISIEELARYPLVTYVFGFDRASEIERAFNRAGLEPRLVFSATSADVLKTYVRLGLGV
GVLASMAIDPNIDRDLVAIDASHLFAHSTTKIGFRKGSFLRSYMYDFIELFAPHLTRDVVEKAVALRDPILIEEMFADRE
LPIR

Sequences:

>Translated_324_residues
MKLQQLRYIAEVVNHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGEQVIAIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIKGFIKRYPKVNLHMHQGTPTQISELAARGEADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAGRSQISIEELARYPLVTYVFGFDRASEIERAFNRAGLEPRLVFSATSADVLKTYVRLGLGV
GVLASMAIDPNIDRDLVAIDASHLFAHSTTKIGFRKGSFLRSYMYDFIELFAPHLTRDVVEKAVALRDPILIEEMFADRE
LPIR
>Mature_324_residues
MKLQQLRYIAEVVNHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVTPAGEQVIAIANDILGKVESI
KKVAEEYTKPDQGELNIATTDTQARYALPHIIKGFIKRYPKVNLHMHQGTPTQISELAARGEADFAIATEAMHLYSDLIM
LPCYHWNRSIVVTRDHPLAGRSQISIEELARYPLVTYVFGFDRASEIERAFNRAGLEPRLVFSATSADVLKTYVRLGLGV
GVLASMAIDPNIDRDLVAIDASHLFAHSTTKIGFRKGSFLRSYMYDFIELFAPHLTRDVVEKAVALRDPILIEEMFADRE
LPIR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=73.9130434782609, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1788296, Length=304, Percent_Identity=40.1315789473684, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI157672245, Length=248, Percent_Identity=29.4354838709677, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790262, Length=232, Percent_Identity=25.8620689655172, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=27.710843373494, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=173, Percent_Identity=23.6994219653179, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=28.1879194630872, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=226, Percent_Identity=22.5663716814159, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36358; Mature: 36358

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIAEVVNHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGEQVIAIANDILGKVESIKKVAEEYTKPDQGELNIATTDTQARYALPHIIKGFIKRYP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
KVNLHMHQGTPTQISELAARGEADFAIATEAMHLYSDLIMLPCYHWNRSIVVTRDHPLAG
CCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RSQISIEELARYPLVTYVFGFDRASEIERAFNRAGLEPRLVFSATSADVLKTYVRLGLGV
CCCCCHHHHHCCCCEEEHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCH
GVLASMAIDPNIDRDLVAIDASHLFAHSTTKIGFRKGSFLRSYMYDFIELFAPHLTRDVV
HHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKAVALRDPILIEEMFADRELPIR
HHHHHHCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLQQLRYIAEVVNHNLNVSATAENLYTSQPGISKQVRMLEDELGIQIFGRSGKHLTHVT
CCHHHHHHHHHHHCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEC
PAGEQVIAIANDILGKVESIKKVAEEYTKPDQGELNIATTDTQARYALPHIIKGFIKRYP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
KVNLHMHQGTPTQISELAARGEADFAIATEAMHLYSDLIMLPCYHWNRSIVVTRDHPLAG
CCEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RSQISIEELARYPLVTYVFGFDRASEIERAFNRAGLEPRLVFSATSADVLKTYVRLGLGV
CCCCCHHHHHCCCCEEEHCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCH
GVLASMAIDPNIDRDLVAIDASHLFAHSTTKIGFRKGSFLRSYMYDFIELFAPHLTRDVV
HHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKAVALRDPILIEEMFADRELPIR
HHHHHHCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]