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The map label for this gene is topA [H]

Identifier: 119774459

GI number: 119774459

Start: 1609167

End: 1611785

Strand: Direct

Name: topA [H]

Synonym: Sama_1322

Alternate gene names: 119774459

Gene position: 1609167-1611785 (Clockwise)

Preceding gene: 119774457

Following gene: 119774461

Centisome position: 37.37

GC content: 53.23

Gene sequence:

>2619_bases
ATGGGTAAATCGCTCGTTATTGTCGAATCGCCGGCGAAAGCCAAGACCATCAACAAGTATCTTGGTAAAGAATTTATTGT
GAAATCGAGTGTTGGCCACATTCGTGATCTGCCAACCTCGTCTGCCGCCGAAGGCAAAGCCAGCACCAAGTCAGCTGCCG
AAGTTCGCAAGATGAATCCTGAAGAAAAGGCCAAATACAAGGCTCAAAAGGACAAGCAAGCCTTGGTTGCCCGAATGGGT
GTTGACCCCGAACATGGATGGCAAGCCAACTACCAGGTACTCCCCGGTAAAGAGAAGGTGGTCAAAGAACTGCAATCTCT
CGCCAGCAGCGCTGACCAAATCTATCTCGCAACCGACTTGGATAGAGAAGGGGAGGCCATTGCCTGGCATTTGCAGGAAG
TGATAGGCGGCGACGAGTCAAGATATAAGCGCGTGGTATTTAACGAAATTACCAAAGGCGCTATTCAGGAGGCCTTTAGC
CGTCCCACTGATTTGAACACCAATATGGTTAACGCCCAGCAAGCTCGCCGGTTCCTAGACCGGGTAGTGGGCTTTATGGT
ATCGCCTTTGCTGTGGAAAAAAGTGGCCCGGGGCTTGTCTGCTGGTCGCGTGCAATCGGTTGCAGTGCGGTTGGTGGTTG
AGCGTGAAGCTGAAATCAAGGCTTTCGTGCCGGAAGAGTTTTGGGATGTTCATGCTACCCTGAGTACACCCGCGGCGGCT
GAGTTGGTGATGGAGGTCAGTCGCTATCGGAATGAGGCTTTTGAACCTGTTACTCAATCAGAGGCCATGGCGGCGGTTAA
TGCGCTTGAAAAAGCGGCTTTCACCGTCACTGACCGTGAGGACAAGGCTACCTCCAGTAAACCAAGCGCTCCCTTTATTA
CCTCCACCCTGCAACAGGCGGCGAGCACCCGTTTGGGCTTTGGGGTGAAGAAGACCATGATGCTGGCCCAAAGGCTCTAC
GAAGCGGGTCACATCACCTATATGCGTACTGACTCAACCAATTTGAGCCAGGAAGCGCTTGAAAATGTGCGAGCGCACAT
TGAAAAGGAATTTGGCAGTAAGTACTTACCCGAGTCTCCTATTCGTTACGGCAGTAAAGAGGGTGCACAAGAGGCTCACG
AAGCCATACGTCCTTCAAACGTTAAGGTAGAGTCGGCAACCCTGATCGATATGGAAAGGGACGCGCAGCGGCTCTATGAG
CTGATTTGGCGGCAATTTGTGGCCTGTCAGATGACGCCTGCCGTTTACGATGCGACCCGGATAACTGCCAAAGCCGGCGA
TTATGAGCTCAAGGCAAGCGGCCGTATTCTCAAATTTGATGGTTGGACCCGTGTACAACCTGCACTGAAGAAAAAGAACG
AAGAAGATAATACCCTGCCGGATGTGCAGGTGGGGGATATGCTCTCGCTTAAGGCACTGGATCCCAAGCAGCACTTCACC
AAGCCCCCAGCGCGCTACAGCGAAGCCTCACTGGTTAAAGAGCTCGAAAAGCGCGGTATTGGCCGTCCATCAACCTACGC
AGCCATCATCTCGACCATTCAGGACCGTGGCTACGTGAAGGTGGAAAACCGCCGTTTCTACGCCGAAAAAATGGGTGAGA
TTGTCACCGAGCGTTTGGTCGAAAGCTTCAGCGAGCTGATGAACTACGACTTTACGGCGGGAATGGAACAGACCCTGGAC
GATGTTGCCAAGGGCGACAAAGACTGGAAAGGCGTGCTGGACGGTTTTTACAGCGGTTTTACTGCCAAGCTGGAGAAAGC
CGAGGCTGAACCCGATGCTGGCGGTATGCGCCCCAATCAAATGGTGCTGACGGATATCCCCTGTCCTACCTGTGGCCGCC
AAATGGGGATCCGTACCGGCACCACAGGTGTGTTCCTGGGCTGTTCAGGTTATGCCTTGCCCCCCAAAGAGCGTTGTAAG
ACGACCCTGAATCTGACGCCGGGTGAAGAAGCCATCAGCGACAGCAGTGAAGATGCCGAGACCGATGCGCTCAGAGCCAT
GCACCGCTGTGGCAAGTGCGGTATGGCCATGGACTCCTACTTGCTGGACGAAACCCGAAAACTTCACGTGTGCGGTAATA
ACCCCGAGTGTGACGGTTATGAGGTCGAAAAAGGCGCATTTAAGCTTAAAGGCTATGAAGGCCCTATTATCGAGTGCGAC
CGCTGTGGCAGTGATATGGAGCTGAAAAACGGCCGATTTGGCAAGTATTTCGGCTGTACCAATGAGAGCTGTAAAAATAC
CCGTAAGCTCCTTAAAAATGGTGAAGCTGCGCCACCGAAGGAGGATCCTATCCATCTTCCTGAGCTGAAATGTACCAAGT
CTGACGCCTATTTTGTGCTCAGAGACGGCGCTGCCGGGATCTTCCTTGCGGCCAGTACTTTCCCACGCTCACGGGAAACC
CGCGCGCCTTTGGTGGAAGAACTGAAGAAATACCGTGAAGCCCTGTGGCCCAAGTACCAGTATCTGGCCGATGCGCCTGT
TGAGGATGCCGAGGGTAACAAGGCGCTGGTGAAATTCAGCCGTAAGACCAAGGAGCAATATGTGGCCACTGAAGTCAATG
GTAAGGCCACCGGGTGGAGCGCTCACTTCAAAGACGGCAAATGGGTTGAGTCCAAGTAA

Upstream 100 bases:

>100_bases
TGGTCCTGATATCTGAGGGAAGCGCTTGAAACTGCCGTTTCAACCCTCTATATATGGGGCCAATTTCTACCTTCTCGAGA
ATTTTGGCGCAAATTAAGCT

Downstream 100 bases:

>100_bases
GATGTTTTACCGGCCAACCCTTCTCAGGTTGGCCATATAAAAAAATCCCCGCAATGCGGGGATTTTTTTATGTGGGAGGG
ATGTAATCAATGATGGCGTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 872; Mature: 871

Protein sequence:

>872_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSAAEGKASTKSAAEVRKMNPEEKAKYKAQKDKQALVARMG
VDPEHGWQANYQVLPGKEKVVKELQSLASSADQIYLATDLDREGEAIAWHLQEVIGGDESRYKRVVFNEITKGAIQEAFS
RPTDLNTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVEREAEIKAFVPEEFWDVHATLSTPAAA
ELVMEVSRYRNEAFEPVTQSEAMAAVNALEKAAFTVTDREDKATSSKPSAPFITSTLQQAASTRLGFGVKKTMMLAQRLY
EAGHITYMRTDSTNLSQEALENVRAHIEKEFGSKYLPESPIRYGSKEGAQEAHEAIRPSNVKVESATLIDMERDAQRLYE
LIWRQFVACQMTPAVYDATRITAKAGDYELKASGRILKFDGWTRVQPALKKKNEEDNTLPDVQVGDMLSLKALDPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKVENRRFYAEKMGEIVTERLVESFSELMNYDFTAGMEQTLD
DVAKGDKDWKGVLDGFYSGFTAKLEKAEAEPDAGGMRPNQMVLTDIPCPTCGRQMGIRTGTTGVFLGCSGYALPPKERCK
TTLNLTPGEEAISDSSEDAETDALRAMHRCGKCGMAMDSYLLDETRKLHVCGNNPECDGYEVEKGAFKLKGYEGPIIECD
RCGSDMELKNGRFGKYFGCTNESCKNTRKLLKNGEAAPPKEDPIHLPELKCTKSDAYFVLRDGAAGIFLAASTFPRSRET
RAPLVEELKKYREALWPKYQYLADAPVEDAEGNKALVKFSRKTKEQYVATEVNGKATGWSAHFKDGKWVESK

Sequences:

>Translated_872_residues
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSAAEGKASTKSAAEVRKMNPEEKAKYKAQKDKQALVARMG
VDPEHGWQANYQVLPGKEKVVKELQSLASSADQIYLATDLDREGEAIAWHLQEVIGGDESRYKRVVFNEITKGAIQEAFS
RPTDLNTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVEREAEIKAFVPEEFWDVHATLSTPAAA
ELVMEVSRYRNEAFEPVTQSEAMAAVNALEKAAFTVTDREDKATSSKPSAPFITSTLQQAASTRLGFGVKKTMMLAQRLY
EAGHITYMRTDSTNLSQEALENVRAHIEKEFGSKYLPESPIRYGSKEGAQEAHEAIRPSNVKVESATLIDMERDAQRLYE
LIWRQFVACQMTPAVYDATRITAKAGDYELKASGRILKFDGWTRVQPALKKKNEEDNTLPDVQVGDMLSLKALDPKQHFT
KPPARYSEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKVENRRFYAEKMGEIVTERLVESFSELMNYDFTAGMEQTLD
DVAKGDKDWKGVLDGFYSGFTAKLEKAEAEPDAGGMRPNQMVLTDIPCPTCGRQMGIRTGTTGVFLGCSGYALPPKERCK
TTLNLTPGEEAISDSSEDAETDALRAMHRCGKCGMAMDSYLLDETRKLHVCGNNPECDGYEVEKGAFKLKGYEGPIIECD
RCGSDMELKNGRFGKYFGCTNESCKNTRKLLKNGEAAPPKEDPIHLPELKCTKSDAYFVLRDGAAGIFLAASTFPRSRET
RAPLVEELKKYREALWPKYQYLADAPVEDAEGNKALVKFSRKTKEQYVATEVNGKATGWSAHFKDGKWVESK
>Mature_871_residues
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSAAEGKASTKSAAEVRKMNPEEKAKYKAQKDKQALVARMGV
DPEHGWQANYQVLPGKEKVVKELQSLASSADQIYLATDLDREGEAIAWHLQEVIGGDESRYKRVVFNEITKGAIQEAFSR
PTDLNTNMVNAQQARRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVEREAEIKAFVPEEFWDVHATLSTPAAAE
LVMEVSRYRNEAFEPVTQSEAMAAVNALEKAAFTVTDREDKATSSKPSAPFITSTLQQAASTRLGFGVKKTMMLAQRLYE
AGHITYMRTDSTNLSQEALENVRAHIEKEFGSKYLPESPIRYGSKEGAQEAHEAIRPSNVKVESATLIDMERDAQRLYEL
IWRQFVACQMTPAVYDATRITAKAGDYELKASGRILKFDGWTRVQPALKKKNEEDNTLPDVQVGDMLSLKALDPKQHFTK
PPARYSEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKVENRRFYAEKMGEIVTERLVESFSELMNYDFTAGMEQTLDD
VAKGDKDWKGVLDGFYSGFTAKLEKAEAEPDAGGMRPNQMVLTDIPCPTCGRQMGIRTGTTGVFLGCSGYALPPKERCKT
TLNLTPGEEAISDSSEDAETDALRAMHRCGKCGMAMDSYLLDETRKLHVCGNNPECDGYEVEKGAFKLKGYEGPIIECDR
CGSDMELKNGRFGKYFGCTNESCKNTRKLLKNGEAAPPKEDPIHLPELKCTKSDAYFVLRDGAAGIFLAASTFPRSRETR
APLVEELKKYREALWPKYQYLADAPVEDAEGNKALVKFSRKTKEQYVATEVNGKATGWSAHFKDGKWVESK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=516, Percent_Identity=27.5193798449612, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI10835218, Length=570, Percent_Identity=25.9649122807018, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI1787529, Length=874, Percent_Identity=70.9382151029748, Blast_Score=1306, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=562, Percent_Identity=24.5551601423488, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=589, Percent_Identity=25.6366723259762, Blast_Score=139, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=511, Percent_Identity=24.853228962818, Blast_Score=109, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=481, Percent_Identity=23.7006237006237, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585251, Length=598, Percent_Identity=25.0836120401338, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640096, Length=503, Percent_Identity=26.441351888668, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96949; Mature: 96818

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSAAEGKASTKSAAEVRKMNP
CCCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCH
EEKAKYKAQKDKQALVARMGVDPEHGWQANYQVLPGKEKVVKELQSLASSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDESRYKRVVFNEITKGAIQEAFSRPTDLNTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVEREAEIKAFVPEEFWDVHATLSTPAAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCHHH
ELVMEVSRYRNEAFEPVTQSEAMAAVNALEKAAFTVTDREDKATSSKPSAPFITSTLQQA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMLAQRLYEAGHITYMRTDSTNLSQEALENVRAHIEKEFGSKYLPESP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVKVESATLIDMERDAQRLYELIWRQFVACQMTPAVYDATR
CCCCCCCHHHHHHHHCCCCCEEEECEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITAKAGDYELKASGRILKFDGWTRVQPALKKKNEEDNTLPDVQVGDMLSLKALDPKQHFT
EEECCCCEEEECCCEEEEECCCCHHCHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKVENRRFYAEKMGEIVTERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
ESFSELMNYDFTAGMEQTLDDVAKGDKDWKGVLDGFYSGFTAKLEKAEAEPDAGGMRPNQ
HHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
MVLTDIPCPTCGRQMGIRTGTTGVFLGCSGYALPPKERCKTTLNLTPGEEAISDSSEDAE
EEEEECCCCCCCHHCCCCCCCCEEEEECCCCCCCCHHHCCEEECCCCCHHHHCCCCCCHH
TDALRAMHRCGKCGMAMDSYLLDETRKLHVCGNNPECDGYEVEKGAFKLKGYEGPIIECD
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEECCCCCCEEECC
RCGSDMELKNGRFGKYFGCTNESCKNTRKLLKNGEAAPPKEDPIHLPELKCTKSDAYFVL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEE
RDGAAGIFLAASTFPRSRETRAPLVEELKKYREALWPKYQYLADAPVEDAEGNKALVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHH
RKTKEQYVATEVNGKATGWSAHFKDGKWVESK
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKEFIVKSSVGHIRDLPTSSAAEGKASTKSAAEVRKMNP
CCCEEEEECCCHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCH
EEKAKYKAQKDKQALVARMGVDPEHGWQANYQVLPGKEKVVKELQSLASSADQIYLATDL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECC
DREGEAIAWHLQEVIGGDESRYKRVVFNEITKGAIQEAFSRPTDLNTNMVNAQQARRFLD
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RVVGFMVSPLLWKKVARGLSAGRVQSVAVRLVVEREAEIKAFVPEEFWDVHATLSTPAAA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCHHH
ELVMEVSRYRNEAFEPVTQSEAMAAVNALEKAAFTVTDREDKATSSKPSAPFITSTLQQA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHH
ASTRLGFGVKKTMMLAQRLYEAGHITYMRTDSTNLSQEALENVRAHIEKEFGSKYLPESP
HHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
IRYGSKEGAQEAHEAIRPSNVKVESATLIDMERDAQRLYELIWRQFVACQMTPAVYDATR
CCCCCCCHHHHHHHHCCCCCEEEECEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITAKAGDYELKASGRILKFDGWTRVQPALKKKNEEDNTLPDVQVGDMLSLKALDPKQHFT
EEECCCCEEEECCCEEEEECCCCHHCHHHHCCCCCCCCCCCCCCCCCEEEECCCCHHHCC
KPPARYSEASLVKELEKRGIGRPSTYAAIISTIQDRGYVKVENRRFYAEKMGEIVTERLV
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
ESFSELMNYDFTAGMEQTLDDVAKGDKDWKGVLDGFYSGFTAKLEKAEAEPDAGGMRPNQ
HHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCC
MVLTDIPCPTCGRQMGIRTGTTGVFLGCSGYALPPKERCKTTLNLTPGEEAISDSSEDAE
EEEEECCCCCCCHHCCCCCCCCEEEEECCCCCCCCHHHCCEEECCCCCHHHHCCCCCCHH
TDALRAMHRCGKCGMAMDSYLLDETRKLHVCGNNPECDGYEVEKGAFKLKGYEGPIIECD
HHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEECCCCCCEEECC
RCGSDMELKNGRFGKYFGCTNESCKNTRKLLKNGEAAPPKEDPIHLPELKCTKSDAYFVL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCEEEE
RDGAAGIFLAASTFPRSRETRAPLVEELKKYREALWPKYQYLADAPVEDAEGNKALVKFS
ECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHH
RKTKEQYVATEVNGKATGWSAHFKDGKWVESK
HHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]