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The map label for this gene is cobD [H]

Identifier: 119773991

GI number: 119773991

Start: 1040123

End: 1041112

Strand: Reverse

Name: cobD [H]

Synonym: Sama_0854

Alternate gene names: 119773991

Gene position: 1041112-1040123 (Counterclockwise)

Preceding gene: 119773992

Following gene: 119773990

Centisome position: 24.18

GC content: 57.37

Gene sequence:

>990_bases
ATGACCGAAAGCCAGTTTGTCAGCCAGCTGATTGCCCTGGATGGAGCCCTGTTTGAAGGCTTCCTTATCATCCTGTGCGC
GCTGCCACTGGCACGACTGGCTCCTTTGCCGAGGGAAATGCAGCCACTGGTGTGGTTTGGCGTGCTGGCAAGAGAGCTTG
CCCGCAAAGTGAATCACCCATCGCGGGGACCGCGCCAACAGCTTATTGCCGGTATTATGGCGACAATACTGCTGGTACTG
CCCTTTTGGCTTATCATCGCCTTTTTGCTGGACTTGGCAGCCTTTCCCTGGTTTTTCGAGTTTTTAATTCTCTACCTCTG
CCTGTCGGATGCCAGTTTTGCCCAGGTCGCCGAAGAGGTGCAGCGGGCACTGGGCCATGGCGATAAAGACAGGGCCAGAA
AGCTGCTGAGACAGTACCTGTCACGGGATACCGCTGAGCTATCCGAGGCAGGGCTTGCCAAGGCCACCATCGAAAAGCTG
GTCACGGCCCCCATCTACGGCACAGCAGGGGTGGTGTTTTTCTATGCCCTGGCCGGTGCGCCCATGGTGCTGCTGGTGCG
AATGCTCAAACAGCTGGAGTTGGTGTGGCCCCCCATGTCACCGGATTTCCGTGCCTTTGTCGCGCCGGTTAATCTACTGG
TCTCGGCACTGCTTTACATTCCTGCCTGGCTATGGAGCCTGACACTGGCCATTGTTGGCGGCCCCAGCGGTTTTAAGGCC
CTGCTAACCCCCAGACGCTGGCAGCCCCTGTCCAATCCCATGCGGGCGGCATATGTTGCCGCGACAGTGCTGAACACAGA
GCTTGGCGGACCACACAAATACCGAGGCAACCGGGTTGCCGTAGATAAGGTCGGCAAAGGCCCCTTGCCCGATACCGCCA
AAATCGGTGAAGCCATAAAATTGTCCAACCGTGCCTGTTTCAGCTGGTTGGCCTTTCTGCTGTTTTTACCCCTTGCATGG
ACCCTGCTCAGATACAGCCAGACGCTGTAA

Upstream 100 bases:

>100_bases
TAATGCCAACAACGACAGCCCGGTCGACTTTGAGTCTTATCTGGTGAAAGCCGGTCATCACTCTGCTCTGATGGTCATGG
CGCTGATTGAAGGCCTGTAA

Downstream 100 bases:

>100_bases
TTTGGTAGCCAACGCACTATAATGGCTCGCCTCAGGGGAATCTCTCCACTTTCCATAACGGGCACTCAACGTGTTAGAAC
AACTGCATATCTCGCTGACA

Product: CobD-like protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTESQFVSQLIALDGALFEGFLIILCALPLARLAPLPREMQPLVWFGVLARELARKVNHPSRGPRQQLIAGIMATILLVL
PFWLIIAFLLDLAAFPWFFEFLILYLCLSDASFAQVAEEVQRALGHGDKDRARKLLRQYLSRDTAELSEAGLAKATIEKL
VTAPIYGTAGVVFFYALAGAPMVLLVRMLKQLELVWPPMSPDFRAFVAPVNLLVSALLYIPAWLWSLTLAIVGGPSGFKA
LLTPRRWQPLSNPMRAAYVAATVLNTELGGPHKYRGNRVAVDKVGKGPLPDTAKIGEAIKLSNRACFSWLAFLLFLPLAW
TLLRYSQTL

Sequences:

>Translated_329_residues
MTESQFVSQLIALDGALFEGFLIILCALPLARLAPLPREMQPLVWFGVLARELARKVNHPSRGPRQQLIAGIMATILLVL
PFWLIIAFLLDLAAFPWFFEFLILYLCLSDASFAQVAEEVQRALGHGDKDRARKLLRQYLSRDTAELSEAGLAKATIEKL
VTAPIYGTAGVVFFYALAGAPMVLLVRMLKQLELVWPPMSPDFRAFVAPVNLLVSALLYIPAWLWSLTLAIVGGPSGFKA
LLTPRRWQPLSNPMRAAYVAATVLNTELGGPHKYRGNRVAVDKVGKGPLPDTAKIGEAIKLSNRACFSWLAFLLFLPLAW
TLLRYSQTL
>Mature_328_residues
TESQFVSQLIALDGALFEGFLIILCALPLARLAPLPREMQPLVWFGVLARELARKVNHPSRGPRQQLIAGIMATILLVLP
FWLIIAFLLDLAAFPWFFEFLILYLCLSDASFAQVAEEVQRALGHGDKDRARKLLRQYLSRDTAELSEAGLAKATIEKLV
TAPIYGTAGVVFFYALAGAPMVLLVRMLKQLELVWPPMSPDFRAFVAPVNLLVSALLYIPAWLWSLTLAIVGGPSGFKAL
LTPRRWQPLSNPMRAAYVAATVLNTELGGPHKYRGNRVAVDKVGKGPLPDTAKIGEAIKLSNRACFSWLAFLLFLPLAWT
LLRYSQTL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 36441; Mature: 36310

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESQFVSQLIALDGALFEGFLIILCALPLARLAPLPREMQPLVWFGVLARELARKVNHP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
SRGPRQQLIAGIMATILLVLPFWLIIAFLLDLAAFPWFFEFLILYLCLSDASFAQVAEEV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QRALGHGDKDRARKLLRQYLSRDTAELSEAGLAKATIEKLVTAPIYGTAGVVFFYALAGA
HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PMVLLVRMLKQLELVWPPMSPDFRAFVAPVNLLVSALLYIPAWLWSLTLAIVGGPSGFKA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LLTPRRWQPLSNPMRAAYVAATVLNTELGGPHKYRGNRVAVDKVGKGPLPDTAKIGEAIK
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEHCCCCCCCCCCHHHHHHHHH
LSNRACFSWLAFLLFLPLAWTLLRYSQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TESQFVSQLIALDGALFEGFLIILCALPLARLAPLPREMQPLVWFGVLARELARKVNHP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]