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The map label for this gene is rstB [H]

Identifier: 119773769

GI number: 119773769

Start: 783561

End: 784757

Strand: Reverse

Name: rstB [H]

Synonym: Sama_0631

Alternate gene names: 119773769

Gene position: 784757-783561 (Counterclockwise)

Preceding gene: 119773770

Following gene: 119773765

Centisome position: 18.22

GC content: 55.3

Gene sequence:

>1197_bases
ATGAAGCCTCAGCTCATCCGGCTGTTTGTTCTTATCATTGCTTCCTGCGCCTTTATCGTTTGGTCCTTTAACCTTATTTA
CAGCGCGTCCGACGAGGCATATGGCTACAGTCTGGATGCCGACGCCCTGTTGAAACAGGGGCGTTTGGGGTTTCGCGAGC
TGCAACCTGGCAGTATTGCCTTTGCCCCCGAGTTACAGACCCGTCTAGACAGTGGTGAAGTCATTTCCCTCACCCACCAG
GATGGCAAAGAGTTTTATTACCTGCTTCTCGATAATGGCCACATCCGGGAAATGGGGCCCGTCAATCCCCAGGCGCAGCA
CAACCAGCTAAACACCCTGATCCCACTCCTTTACGCCTCGCTTGCGCTGGCGATATTGCTGCTGATCGGAAGGGTGTTCA
AGGATTTGAGTCTGCTTCAGTCACAGGCACTGAGTTTTGGTGCGCACCCCAAGCGGATTAAAATCGGCATCGGGCACCGC
TCGGCAATTTATCCCCTCGCCAAGAGCTTTGAGACCATGACAGGGCAAATCGTCGACTTTCTGGAGCTGCACCGGGATCT
GTCGCGGATCATGTCCCACGAGATACGCACGCCCTTATCACGGATGCGGTTTGTTCTCGAGCTGGCCAAAGCACAATTGT
CTGAAAAACACAGCGCCCGCCTGGAAGAGGACATCAATCAAATCGAGGCCCTGACCAAGAGCTACCTGTCGTTTGCACGG
GTTGAGCACCAGCAGCCCCACCAACAGCGCGAATGGGTGAATGCGCCTGAATTTATCAATGCCGTGGCAGATAATTTTGC
CGTGTATGCCCCCAAATACAGGGTGCACTGTCAGGTGGACTGCGCCCTCAGTGACGACAAGGTGTTGATTGACCCACTGA
GTATGGATCTGGCCAGCCAAAACCTGATAGCCAACGCCCTGCGATTCGCCAGCGGTAATATTAAGCTGACGCTGGTCGCA
GAGACCCGCCATTGGCGGCTAACCGTGGAAGATGATGGTCCGGGCATACAGGAGCCGGGCAAAGACTTGCGCCAGGCGTT
TCAGCGGGGCAGTAAACAGCAAAGTGCCGGATTTGGCCTGGGCCTCTATATTGTCCGCAAAATTGCCATCTGGCATCAGG
GCAAACTGACGGTGGAAAACTCGGCCGAACTTGGCGGTGCCTCCATCAGTCTGCGCTGGCGCAGGCCGCTTCCTTGA

Upstream 100 bases:

>100_bases
CCCGCCTGAGACGTAAGCTGGAAAACATCGGTATCCCGGGTCTGGCCATTCGTACCGTGCACGGCCGGGGTTATCTGCTG
AGCTTTGCGACTGACGAGAT

Downstream 100 bases:

>100_bases
GCAGCAGACACAGCACCTCAAAGTGGTCACTGTGGGGGAACATGTCGAACAGCTGTACCCGGGCGATGCGATAGCCCGAG
ATCAGGGCCAGATCTTTAGC

Product: signal transduction histidine kinase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKPQLIRLFVLIIASCAFIVWSFNLIYSASDEAYGYSLDADALLKQGRLGFRELQPGSIAFAPELQTRLDSGEVISLTHQ
DGKEFYYLLLDNGHIREMGPVNPQAQHNQLNTLIPLLYASLALAILLLIGRVFKDLSLLQSQALSFGAHPKRIKIGIGHR
SAIYPLAKSFETMTGQIVDFLELHRDLSRIMSHEIRTPLSRMRFVLELAKAQLSEKHSARLEEDINQIEALTKSYLSFAR
VEHQQPHQQREWVNAPEFINAVADNFAVYAPKYRVHCQVDCALSDDKVLIDPLSMDLASQNLIANALRFASGNIKLTLVA
ETRHWRLTVEDDGPGIQEPGKDLRQAFQRGSKQQSAGFGLGLYIVRKIAIWHQGKLTVENSAELGGASISLRWRRPLP

Sequences:

>Translated_398_residues
MKPQLIRLFVLIIASCAFIVWSFNLIYSASDEAYGYSLDADALLKQGRLGFRELQPGSIAFAPELQTRLDSGEVISLTHQ
DGKEFYYLLLDNGHIREMGPVNPQAQHNQLNTLIPLLYASLALAILLLIGRVFKDLSLLQSQALSFGAHPKRIKIGIGHR
SAIYPLAKSFETMTGQIVDFLELHRDLSRIMSHEIRTPLSRMRFVLELAKAQLSEKHSARLEEDINQIEALTKSYLSFAR
VEHQQPHQQREWVNAPEFINAVADNFAVYAPKYRVHCQVDCALSDDKVLIDPLSMDLASQNLIANALRFASGNIKLTLVA
ETRHWRLTVEDDGPGIQEPGKDLRQAFQRGSKQQSAGFGLGLYIVRKIAIWHQGKLTVENSAELGGASISLRWRRPLP
>Mature_398_residues
MKPQLIRLFVLIIASCAFIVWSFNLIYSASDEAYGYSLDADALLKQGRLGFRELQPGSIAFAPELQTRLDSGEVISLTHQ
DGKEFYYLLLDNGHIREMGPVNPQAQHNQLNTLIPLLYASLALAILLLIGRVFKDLSLLQSQALSFGAHPKRIKIGIGHR
SAIYPLAKSFETMTGQIVDFLELHRDLSRIMSHEIRTPLSRMRFVLELAKAQLSEKHSARLEEDINQIEALTKSYLSFAR
VEHQQPHQQREWVNAPEFINAVADNFAVYAPKYRVHCQVDCALSDDKVLIDPLSMDLASQNLIANALRFASGNIKLTLVA
ETRHWRLTVEDDGPGIQEPGKDLRQAFQRGSKQQSAGFGLGLYIVRKIAIWHQGKLTVENSAELGGASISLRWRRPLP

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=334, Percent_Identity=27.8443113772455, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789808, Length=243, Percent_Identity=29.6296296296296, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790346, Length=225, Percent_Identity=27.1111111111111, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=26.6094420600858, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=27.5229357798165, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=291, Percent_Identity=23.3676975945017, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=219, Percent_Identity=29.2237442922374, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790551, Length=246, Percent_Identity=26.8292682926829, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44744; Mature: 44744

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQLIRLFVLIIASCAFIVWSFNLIYSASDEAYGYSLDADALLKQGRLGFRELQPGSIA
CCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
FAPELQTRLDSGEVISLTHQDGKEFYYLLLDNGHIREMGPVNPQAQHNQLNTLIPLLYAS
ECCHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LALAILLLIGRVFKDLSLLQSQALSFGAHPKRIKIGIGHRSAIYPLAKSFETMTGQIVDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
LELHRDLSRIMSHEIRTPLSRMRFVLELAKAQLSEKHSARLEEDINQIEALTKSYLSFAR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEHQQPHQQREWVNAPEFINAVADNFAVYAPKYRVHCQVDCALSDDKVLIDPLSMDLASQ
HHHCCCHHHHHHCCCHHHHHHHHCCCEEECCCEEEEEEEEEEECCCCEEECCHHHHHHHH
NLIANALRFASGNIKLTLVAETRHWRLTVEDDGPGIQEPGKDLRQAFQRGSKQQSAGFGL
HHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHCCCCH
GLYIVRKIAIWHQGKLTVENSAELGGASISLRWRRPLP
HHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKPQLIRLFVLIIASCAFIVWSFNLIYSASDEAYGYSLDADALLKQGRLGFRELQPGSIA
CCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEE
FAPELQTRLDSGEVISLTHQDGKEFYYLLLDNGHIREMGPVNPQAQHNQLNTLIPLLYAS
ECCHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LALAILLLIGRVFKDLSLLQSQALSFGAHPKRIKIGIGHRSAIYPLAKSFETMTGQIVDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
LELHRDLSRIMSHEIRTPLSRMRFVLELAKAQLSEKHSARLEEDINQIEALTKSYLSFAR
HHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEHQQPHQQREWVNAPEFINAVADNFAVYAPKYRVHCQVDCALSDDKVLIDPLSMDLASQ
HHHCCCHHHHHHCCCHHHHHHHHCCCEEECCCEEEEEEEEEEECCCCEEECCHHHHHHHH
NLIANALRFASGNIKLTLVAETRHWRLTVEDDGPGIQEPGKDLRQAFQRGSKQQSAGFGL
HHHHHHHHHCCCCEEEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHCCCCH
GLYIVRKIAIWHQGKLTVENSAELGGASISLRWRRPLP
HHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]