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The map label for this gene is phoB [H]

Identifier: 119773711

GI number: 119773711

Start: 713799

End: 715277

Strand: Reverse

Name: phoB [H]

Synonym: Sama_0573

Alternate gene names: 119773711

Gene position: 715277-713799 (Counterclockwise)

Preceding gene: 119773712

Following gene: 119773707

Centisome position: 16.61

GC content: 56.66

Gene sequence:

>1479_bases
ATGTCATCGAAATTATTGGCCCTGTTGCTGCTGGCCCTGCCCCTGACAGCCGCTGCGGCCGATCCGCTTAAAACCCCGTC
CAAGCCCAAGAACATGGTCATCATGATTGGCGATGGCATGGGGCCGGCTTACACCTCGGCCTATCGTTATTTTAAAGACA
ACCCGGACACCGAAGAAATCGAGCAAACCGTGTTCGACCGTCTACTGGCGGGGATGGCGAGCACCTACCCGGCACCCGTG
TCCGGCTACGTGACCGACTCGGCCGCCGCTGCCACGGCGTTGGCAACCGGCCACAAGAGCTATAACGGCGCCATTTCGGT
GGATGTGAACAAAGCTCCCCTGCCCACCCTGTTTGAGAAAGCCCGGGAAGCCGGTTTAGCCACGGGCGTTGCCGTCTCCA
GCCAGATAAACCATGCCACTCCGGCCGCATTTCTGGCCCACAATGAAAGCCGCAAAAATTACGATGAAATAGCCAGCGCC
ATATTGGGCAGTGATGCCCAGGTGCTGCTCGGCGGCGGTCAAAAGTATTTTGATGAGTCATTGCTCGCCGACTACCGCGC
CAAAGGCTATCAGCATCTCACCGACATCGGCGCGCTCGACACCATTAGCAGCGGTAAGGTGTTGGGCCTCTTTGCCGAAG
TGCAATTGCCCTGGGTGATAGATAACCCGGAATCACACCAGCTTTCTCGTCTCACGGCCAAAGCGCTGGAGCTTCTCTCC
CAAAACGACAAAGGTTTTGTCCTGTTGGTGGAAGGCAGTCTGATTGATTGGGCGGGCCACAACAATGACATAGTGACTGC
CATGGCCGAGATGGACGAGTTTGCCCGCGCCATTGAGGTGGTGGAAGAATTTGTTCGCACCCGTGGCGATACCCTGATGG
TGATTACTGCCGATCACAATACCGGCGGTCTGAGCATAGGGGCAGATGACCAATATCTGTGGGATGCCGCACTGCTGCGT
GGAGTGAAGGCCAGTCCCGACGCCATGGCAGCGGCTATCATCGCCGGCGATGTCTGGCAGGAGCAGCTTGAAACACAATT
GGGCTTTACCCTGGAGTCAGATGAACAAGCAGCGATTTCACGGGCAAGAATGCAGGGGCAGGACGTTTTAAGCAGTGCCA
TAAAAACTATGCTGGATAAACGCAGCTATACCGGCTGGACCAGTGGCGGACACACAGCACTCGATGTGCAGGTATTTGCC
CAAGGCCCCGCATCCGGACTCTTTATCGGTCACCAGGACAACACTGAAATCGCCCAGAAGATGATGAGCCTGCTACCTAA
ACCTAAAAAGGCCGCCGCTGTGGTCGAACCCACAGCCCCGTCGATAACCTTGCCACCGGAAGCGCCTGTCAGCGAAGCGC
CCCTGGTGAACCCAGTTGTGCCGGAAACCGCACAGCCACAGGAAACAACACAGGATGACGCGTTCAACCCAGGCTCTGAC
GTCAGTCAAGAGACTGAGATTAGCGTAACCACTCACTAA

Upstream 100 bases:

>100_bases
TCGACGTGGATATCACGGGCATGCTGATGAACGCCATCGAAGCCCGTATCAACAAGAAATAACCGCGGGCGCCTTGCGCC
CTTCTGCCTTGGGGAACCCT

Downstream 100 bases:

>100_bases
CCCGCCATTTTGCCCAGGAGTTCGCCATCGCCGCTCCTGGGCCACTCCCGCCCTTCAGTAATGCCCTTCAGGATTTCCAT
CGCCCTCTCTTCACAAATGG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSSKLLALLLLALPLTAAAADPLKTPSKPKNMVIMIGDGMGPAYTSAYRYFKDNPDTEEIEQTVFDRLLAGMASTYPAPV
SGYVTDSAAAATALATGHKSYNGAISVDVNKAPLPTLFEKAREAGLATGVAVSSQINHATPAAFLAHNESRKNYDEIASA
ILGSDAQVLLGGGQKYFDESLLADYRAKGYQHLTDIGALDTISSGKVLGLFAEVQLPWVIDNPESHQLSRLTAKALELLS
QNDKGFVLLVEGSLIDWAGHNNDIVTAMAEMDEFARAIEVVEEFVRTRGDTLMVITADHNTGGLSIGADDQYLWDAALLR
GVKASPDAMAAAIIAGDVWQEQLETQLGFTLESDEQAAISRARMQGQDVLSSAIKTMLDKRSYTGWTSGGHTALDVQVFA
QGPASGLFIGHQDNTEIAQKMMSLLPKPKKAAAVVEPTAPSITLPPEAPVSEAPLVNPVVPETAQPQETTQDDAFNPGSD
VSQETEISVTTH

Sequences:

>Translated_492_residues
MSSKLLALLLLALPLTAAAADPLKTPSKPKNMVIMIGDGMGPAYTSAYRYFKDNPDTEEIEQTVFDRLLAGMASTYPAPV
SGYVTDSAAAATALATGHKSYNGAISVDVNKAPLPTLFEKAREAGLATGVAVSSQINHATPAAFLAHNESRKNYDEIASA
ILGSDAQVLLGGGQKYFDESLLADYRAKGYQHLTDIGALDTISSGKVLGLFAEVQLPWVIDNPESHQLSRLTAKALELLS
QNDKGFVLLVEGSLIDWAGHNNDIVTAMAEMDEFARAIEVVEEFVRTRGDTLMVITADHNTGGLSIGADDQYLWDAALLR
GVKASPDAMAAAIIAGDVWQEQLETQLGFTLESDEQAAISRARMQGQDVLSSAIKTMLDKRSYTGWTSGGHTALDVQVFA
QGPASGLFIGHQDNTEIAQKMMSLLPKPKKAAAVVEPTAPSITLPPEAPVSEAPLVNPVVPETAQPQETTQDDAFNPGSD
VSQETEISVTTH
>Mature_491_residues
SSKLLALLLLALPLTAAAADPLKTPSKPKNMVIMIGDGMGPAYTSAYRYFKDNPDTEEIEQTVFDRLLAGMASTYPAPVS
GYVTDSAAAATALATGHKSYNGAISVDVNKAPLPTLFEKAREAGLATGVAVSSQINHATPAAFLAHNESRKNYDEIASAI
LGSDAQVLLGGGQKYFDESLLADYRAKGYQHLTDIGALDTISSGKVLGLFAEVQLPWVIDNPESHQLSRLTAKALELLSQ
NDKGFVLLVEGSLIDWAGHNNDIVTAMAEMDEFARAIEVVEEFVRTRGDTLMVITADHNTGGLSIGADDQYLWDAALLRG
VKASPDAMAAAIIAGDVWQEQLETQLGFTLESDEQAAISRARMQGQDVLSSAIKTMLDKRSYTGWTSGGHTALDVQVFAQ
GPASGLFIGHQDNTEIAQKMMSLLPKPKKAAAVVEPTAPSITLPPEAPVSEAPLVNPVVPETAQPQETTQDDAFNPGSDV
SQETEISVTTH

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=333, Percent_Identity=28.8288288288288, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI94721246, Length=497, Percent_Identity=26.5593561368209, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157266296, Length=497, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI294660770, Length=329, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI294660772, Length=275, Percent_Identity=28.7272727272727, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI48994877, Length=343, Percent_Identity=34.402332361516, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320689, Length=487, Percent_Identity=31.6221765913758, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21355981, Length=459, Percent_Identity=27.2331154684096, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355149, Length=337, Percent_Identity=27.299703264095, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19921912, Length=342, Percent_Identity=29.5321637426901, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI18859923, Length=430, Percent_Identity=28.6046511627907, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355151, Length=459, Percent_Identity=25.2723311546841, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24651554, Length=337, Percent_Identity=29.673590504451, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585213, Length=341, Percent_Identity=28.4457478005865, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24657835, Length=336, Percent_Identity=27.9761904761905, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24657842, Length=461, Percent_Identity=26.6811279826464, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI85725200, Length=331, Percent_Identity=26.5861027190332, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI85815833, Length=331, Percent_Identity=26.5861027190332, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21358067, Length=340, Percent_Identity=25.5882352941176, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24657827, Length=467, Percent_Identity=24.6252676659529, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 52308; Mature: 52177

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLLALLLLALPLTAAAADPLKTPSKPKNMVIMIGDGMGPAYTSAYRYFKDNPDTEEI
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHH
EQTVFDRLLAGMASTYPAPVSGYVTDSAAAATALATGHKSYNGAISVDVNKAPLPTLFEK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
AREAGLATGVAVSSQINHATPAAFLAHNESRKNYDEIASAILGSDAQVLLGGGQKYFDES
HHHCCCHHHHHHHCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHH
LLADYRAKGYQHLTDIGALDTISSGKVLGLFAEVQLPWVIDNPESHQLSRLTAKALELLS
HHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEEEECEEECCCCCHHHHHHHHHHHHHHC
QNDKGFVLLVEGSLIDWAGHNNDIVTAMAEMDEFARAIEVVEEFVRTRGDTLMVITADHN
CCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TGGLSIGADDQYLWDAALLRGVKASPDAMAAAIIAGDVWQEQLETQLGFTLESDEQAAIS
CCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHH
RARMQGQDVLSSAIKTMLDKRSYTGWTSGGHTALDVQVFAQGPASGLFIGHQDNTEIAQK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCHHHHHH
MMSLLPKPKKAAAVVEPTAPSITLPPEAPVSEAPLVNPVVPETAQPQETTQDDAFNPGSD
HHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSQETEISVTTH
CCCCCEEEEECC
>Mature Secondary Structure 
SSKLLALLLLALPLTAAAADPLKTPSKPKNMVIMIGDGMGPAYTSAYRYFKDNPDTEEI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHHH
EQTVFDRLLAGMASTYPAPVSGYVTDSAAAATALATGHKSYNGAISVDVNKAPLPTLFEK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHH
AREAGLATGVAVSSQINHATPAAFLAHNESRKNYDEIASAILGSDAQVLLGGGQKYFDES
HHHCCCHHHHHHHCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHH
LLADYRAKGYQHLTDIGALDTISSGKVLGLFAEVQLPWVIDNPESHQLSRLTAKALELLS
HHHHHHHCCHHHHHHCCCCCCCCCCCEEEEEEEEEECEEECCCCCHHHHHHHHHHHHHHC
QNDKGFVLLVEGSLIDWAGHNNDIVTAMAEMDEFARAIEVVEEFVRTRGDTLMVITADHN
CCCCCEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TGGLSIGADDQYLWDAALLRGVKASPDAMAAAIIAGDVWQEQLETQLGFTLESDEQAAIS
CCCEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHH
RARMQGQDVLSSAIKTMLDKRSYTGWTSGGHTALDVQVFAQGPASGLFIGHQDNTEIAQK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCCCHHHHHH
MMSLLPKPKKAAAVVEPTAPSITLPPEAPVSEAPLVNPVVPETAQPQETTQDDAFNPGSD
HHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VSQETEISVTTH
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]