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The map label for this gene is gshB [H]

Identifier: 119773712

GI number: 119773712

Start: 715316

End: 716266

Strand: Reverse

Name: gshB [H]

Synonym: Sama_0574

Alternate gene names: 119773712

Gene position: 716266-715316 (Counterclockwise)

Preceding gene: 119773713

Following gene: 119773711

Centisome position: 16.63

GC content: 55.63

Gene sequence:

>951_bases
ATGATTAAACTCGGCATAGTGATGGACCCCATCAGCGACATCAACATCAAGAAAGACTCCAGCTTTGCCATGCTGATGGC
CGCCCAAAGTCGCGGCTATGAGCTGTTTTATATGGAAATGCAGGACCTGGCCATGGTCAAGGGTGAGGCCCGCGCCCGCA
TGCATCCCCTCAAGGTAATGCAGAACGCCGATTGCTGGTTTGAATTGGGCGATGCAGTTGAGCAGCCGCTGGCCGAGCTT
GATGTCATCCTGATGCGTAAAGACCCGCCATTCGACACCGAATATATTTACGCCACTTATATGTTGGAACGCGCCGAAGA
GGCGGGCGTGTTGATTGTCAACAAGCCCCAGAGCCTGCGGGACGCCAACGAAAAGCTGTTTACCGCCTGGTTCAGCGAGT
TTACTCCACTGACCATGGTCACCCGCGATGCTGGCCGCATCCGTGAGTTCCACGCCGAGCATGGTGACATTATCTTAAAG
CCGCTGGATGGCATGGGCGGCGCCTCTATCTTTCGCGTCAAACAGGACGACCCGAACCTTGGCGTCATCATAGAAACCCT
CACCAACCACGGTAATTGCTACGCCATGGCTCAGGTGTTTATTCCTGAAATCACCGCGGGCGACAAACGTATTCTGGTGG
TGGATGGTGAGCCAGTTCCATATTGCCTGGCCCGTATCCCCAAGCAGGGTGAAACCCGGGGTAATCTTGCTGCCGGTGGC
CGTGGAGTAGCACAGCCGCTGTCTGACGCCGACTGGCGCATTGCCAAGGCTCTGGGGCCGGAACTCAAGCGCCGGGGTCT
GATTTTTGTGGGTCTGGACGTGATTGGCGATAAACTCACCGAAATCAACGTGACCAGCCCGACCTGTATCCGCGAAATCG
AAGCGGCCTTCGACGTGGATATCACGGGCATGCTGATGAACGCCATCGAAGCCCGTATCAACAAGAAATAA

Upstream 100 bases:

>100_bases
ACTTCGTACTGAAACGGCCGCGCTAACCGCCATTACGGCACTGCAAATGAAATTTGGCGATCTGGGTTAAACCCGGCTCC
CCCGCATGAGAGGACGCAAT

Downstream 100 bases:

>100_bases
CCGCGGGCGCCTTGCGCCCTTCTGCCTTGGGGAACCCTATGTCATCGAAATTATTGGCCCTGTTGCTGCTGGCCCTGCCC
CTGACAGCCGCTGCGGCCGA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYELFYMEMQDLAMVKGEARARMHPLKVMQNADCWFELGDAVEQPLAEL
DVILMRKDPPFDTEYIYATYMLERAEEAGVLIVNKPQSLRDANEKLFTAWFSEFTPLTMVTRDAGRIREFHAEHGDIILK
PLDGMGGASIFRVKQDDPNLGVIIETLTNHGNCYAMAQVFIPEITAGDKRILVVDGEPVPYCLARIPKQGETRGNLAAGG
RGVAQPLSDADWRIAKALGPELKRRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVDITGMLMNAIEARINKK

Sequences:

>Translated_316_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYELFYMEMQDLAMVKGEARARMHPLKVMQNADCWFELGDAVEQPLAEL
DVILMRKDPPFDTEYIYATYMLERAEEAGVLIVNKPQSLRDANEKLFTAWFSEFTPLTMVTRDAGRIREFHAEHGDIILK
PLDGMGGASIFRVKQDDPNLGVIIETLTNHGNCYAMAQVFIPEITAGDKRILVVDGEPVPYCLARIPKQGETRGNLAAGG
RGVAQPLSDADWRIAKALGPELKRRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVDITGMLMNAIEARINKK
>Mature_316_residues
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYELFYMEMQDLAMVKGEARARMHPLKVMQNADCWFELGDAVEQPLAEL
DVILMRKDPPFDTEYIYATYMLERAEEAGVLIVNKPQSLRDANEKLFTAWFSEFTPLTMVTRDAGRIREFHAEHGDIILK
PLDGMGGASIFRVKQDDPNLGVIIETLTNHGNCYAMAQVFIPEITAGDKRILVVDGEPVPYCLARIPKQGETRGNLAAGG
RGVAQPLSDADWRIAKALGPELKRRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVDITGMLMNAIEARINKK

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=316, Percent_Identity=72.1518987341772, Blast_Score=477, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35150; Mature: 35150

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYELFYMEMQDLAMVKGEARARMHPLKVM
CEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHCCCHHHHCCHHHHH
QNADCWFELGDAVEQPLAELDVILMRKDPPFDTEYIYATYMLERAEEAGVLIVNKPQSLR
CCCCHHEEHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHH
DANEKLFTAWFSEFTPLTMVTRDAGRIREFHAEHGDIILKPLDGMGGASIFRVKQDDPNL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTNHGNCYAMAQVFIPEITAGDKRILVVDGEPVPYCLARIPKQGETRGNLAAGG
EEEEEEECCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
RGVAQPLSDADWRIAKALGPELKRRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVD
CCCCCCCCCCCHHHHHHCCHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHCCC
ITGMLMNAIEARINKK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLGIVMDPISDINIKKDSSFAMLMAAQSRGYELFYMEMQDLAMVKGEARARMHPLKVM
CEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHCCCHHHHCCHHHHH
QNADCWFELGDAVEQPLAELDVILMRKDPPFDTEYIYATYMLERAEEAGVLIVNKPQSLR
CCCCHHEEHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCHHHH
DANEKLFTAWFSEFTPLTMVTRDAGRIREFHAEHGDIILKPLDGMGGASIFRVKQDDPNL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCC
GVIIETLTNHGNCYAMAQVFIPEITAGDKRILVVDGEPVPYCLARIPKQGETRGNLAAGG
EEEEEEECCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCC
RGVAQPLSDADWRIAKALGPELKRRGLIFVGLDVIGDKLTEINVTSPTCIREIEAAFDVD
CCCCCCCCCCCHHHHHHCCHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHHCCC
ITGMLMNAIEARINKK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]