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The map label for this gene is epsE [H]

Identifier: 119773597

GI number: 119773597

Start: 563253

End: 565016

Strand: Direct

Name: epsE [H]

Synonym: Sama_0457

Alternate gene names: 119773597

Gene position: 563253-565016 (Clockwise)

Preceding gene: 119773596

Following gene: 119773598

Centisome position: 13.08

GC content: 55.44

Gene sequence:

>1764_bases
ATGAAACCAAAACTCAAGATGCGCCTTGGGGATTTACTGGTCTCGGAACAAATCATTGGTGAGGATCAGCTGACCGCTGC
ACTTGCGCAGCAAAAGAGCACCGGCCGAAAATTGGGCCGCACCCTGATTGATATGGGATATATCGCAGAAGATCAGCTGC
TTAAATTCCTGTCGCAACAGCTGAATATTCCCTATCTCGACATCAGCCGCCGTCCTGTGCCTCCCGCCGTAGTGAACCTG
CTGCCAGAAGTTCAGGCCCGCCGCTACCGGGCCCTGGTGATTGAAGATAAGGGGGATGCGGTGGTGGTCGCCATGAGCGA
TCCGGCGGATCTGCAGGCACTGGACAACCTTGAAGTACTGCTGGCACCCAAGCGTCTCGATTTGGTGGTCGCTCCCGAGG
CCCAGCTGATGCAGGCCTTTGACAACCTGTACCGCCGCACTGAACAAATCGCTCAGATGGCAGGGCAGTTGGAAGAAGAG
TATGCCGCAGACGAGGCATTTGACCTTGCCGCGCTGACCGCTGGCGACAGCGACAGTGAAACCACTGTGGTTAAACTGCT
GCAATCGATTTTTGAAGACGCAGTGCAGATGCGGGCATCCGACATCCACATTGAACCCGGCGATAAATCGCTGCGCATCC
GTCAGCGCGTGGATGGCCACTTGCAGGAAACCATACTAAACGAGGTGACCATAGCCGCGGCGCTCGTGCTGCGTCTCAAG
CTGATGGCCGGGTTGGATATCTCCGAAAAACGTTTGCCGCAGGATGGTCGTTTCCATATGGAAGTTAAAGGCCACAAAAT
AGACGTGCGTATCTCCACCATGCCGATTTATCACGGCGAGTCTGTGGTGATGCGTCTTCTGGACCAGTCGGCCGGTTTGT
TGACCCTGAATGAAACGGGTATGCCGGATGCGATTTTGCAGCGGGTACGCAAACAAATTAAGCGCCCCCACGGCATGTTA
TTGGTGACAGGCCCTACGGGTAGCGGCAAAACCACTACTCTCTACGGCATCCTGAGTGAACTCAATACGGCCGATCGCAA
GATAATCACGGTTGAAGATCCGGTGGAATATCAGCTGCCACGGATCAATCAGGTGCAGGTTAACCACAAGATAGGTCTCA
ACTTCTCCAACGTGCTGCGCACCACATTACGTCAGGACCCCGACATCATCATGGTGGGGGAGATGCGTGACCACGAAACC
GTAGAAATCGGCTTGCGGGGCGCGCTGACGGGTCACTTTGTGCTCTCAACCCTGCACACCAACGATGCCGTAACCTCGGC
GCTGAGACTTCTGGACATGGGCGCAGCAAGCTACTTGGTGGCCAGTGCACTGCGGGTCATCATTGCCCAGCGATTGGTGC
GCCGCGTATGCCAAAATTGCGCCGTGGATTATCAACTGACCAGTCAGGACAAGGCTTGGCTGACCAGTGTCAGCAAAGGA
GTGGATTTTTCTCAGGCCCGTTTTCGCATGGGCACTGGCTGCCAAAGCTGTAGTGGCACAGGTTACCGAGGTCGTATTGG
TATTTTCGAGCTGCTGGAGCTGGATGAAGCCATGGTGGATGCCATGCGCACCGGCAACCCTCAGGCATTTGCCCAGGCGG
CTTACGCCAGCCCTAACTTTACTCCCTTGCTCATCTCGGCACTCAATTACCTTAAGCAGGGCATGACCACGATAGAAGAG
GTTGCCAGACTGGTGGAGGATGTCAGCGAAACCCAGATACCACTGTCGCAGGAAATGGCCCAGTTTGATGGTGCAGGAGC
CTGA

Upstream 100 bases:

>100_bases
ATGCCCGTTATCACTATCAGCGTGCCCTTGGCACTGCCGGGTTATCATCCGACGCCCGCGCCTACATAGAAAACAGATTG
ATGCAGTTGGGAGATAACCA

Downstream 100 bases:

>100_bases
CGATGCCGGTGTACAAGTATCGGGGGCGAAACGCCCAGGGACAGCAGGTAACAGGAGAGCTTGATGCTGCCTCCGAGAAT
GCCGCCGCCGATCAGTTGCT

Product: MSHA biogenesis protein MshE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKPKLKMRLGDLLVSEQIIGEDQLTAALAQQKSTGRKLGRTLIDMGYIAEDQLLKFLSQQLNIPYLDISRRPVPPAVVNL
LPEVQARRYRALVIEDKGDAVVVAMSDPADLQALDNLEVLLAPKRLDLVVAPEAQLMQAFDNLYRRTEQIAQMAGQLEEE
YAADEAFDLAALTAGDSDSETTVVKLLQSIFEDAVQMRASDIHIEPGDKSLRIRQRVDGHLQETILNEVTIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEVKGHKIDVRISTMPIYHGESVVMRLLDQSAGLLTLNETGMPDAILQRVRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDHET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAVDYQLTSQDKAWLTSVSKG
VDFSQARFRMGTGCQSCSGTGYRGRIGIFELLELDEAMVDAMRTGNPQAFAQAAYASPNFTPLLISALNYLKQGMTTIEE
VARLVEDVSETQIPLSQEMAQFDGAGA

Sequences:

>Translated_587_residues
MKPKLKMRLGDLLVSEQIIGEDQLTAALAQQKSTGRKLGRTLIDMGYIAEDQLLKFLSQQLNIPYLDISRRPVPPAVVNL
LPEVQARRYRALVIEDKGDAVVVAMSDPADLQALDNLEVLLAPKRLDLVVAPEAQLMQAFDNLYRRTEQIAQMAGQLEEE
YAADEAFDLAALTAGDSDSETTVVKLLQSIFEDAVQMRASDIHIEPGDKSLRIRQRVDGHLQETILNEVTIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEVKGHKIDVRISTMPIYHGESVVMRLLDQSAGLLTLNETGMPDAILQRVRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDHET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAVDYQLTSQDKAWLTSVSKG
VDFSQARFRMGTGCQSCSGTGYRGRIGIFELLELDEAMVDAMRTGNPQAFAQAAYASPNFTPLLISALNYLKQGMTTIEE
VARLVEDVSETQIPLSQEMAQFDGAGA
>Mature_587_residues
MKPKLKMRLGDLLVSEQIIGEDQLTAALAQQKSTGRKLGRTLIDMGYIAEDQLLKFLSQQLNIPYLDISRRPVPPAVVNL
LPEVQARRYRALVIEDKGDAVVVAMSDPADLQALDNLEVLLAPKRLDLVVAPEAQLMQAFDNLYRRTEQIAQMAGQLEEE
YAADEAFDLAALTAGDSDSETTVVKLLQSIFEDAVQMRASDIHIEPGDKSLRIRQRVDGHLQETILNEVTIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEVKGHKIDVRISTMPIYHGESVVMRLLDQSAGLLTLNETGMPDAILQRVRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDHET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAVDYQLTSQDKAWLTSVSKG
VDFSQARFRMGTGCQSCSGTGYRGRIGIFELLELDEAMVDAMRTGNPQAFAQAAYASPNFTPLLISALNYLKQGMTTIEE
VARLVEDVSETQIPLSQEMAQFDGAGA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=449, Percent_Identity=42.5389755011136, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1786296, Length=478, Percent_Identity=36.6108786610879, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=39.1752577319588, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 64810; Mature: 64810

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKLKMRLGDLLVSEQIIGEDQLTAALAQQKSTGRKLGRTLIDMGYIAEDQLLKFLSQQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LNIPYLDISRRPVPPAVVNLLPEVQARRYRALVIEDKGDAVVVAMSDPADLQALDNLEVL
CCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHCCCEEE
LAPKRLDLVVAPEAQLMQAFDNLYRRTEQIAQMAGQLEEEYAADEAFDLAALTAGDSDSE
EECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCH
TTVVKLLQSIFEDAVQMRASDIHIEPGDKSLRIRQRVDGHLQETILNEVTIAAALVLRLK
HHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEVKGHKIDVRISTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECCEEEEEEEEEEEECCHHHHHHHHHCCCCEEEECCCC
MPDAILQRVRKQIKRPHGMLLVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDHETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHCC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAVDYQLTSQDKAWLTSVSKG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCC
VDFSQARFRMGTGCQSCSGTGYRGRIGIFELLELDEAMVDAMRTGNPQAFAQAAYASPNF
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
TPLLISALNYLKQGMTTIEEVARLVEDVSETQIPLSQEMAQFDGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKPKLKMRLGDLLVSEQIIGEDQLTAALAQQKSTGRKLGRTLIDMGYIAEDQLLKFLSQQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LNIPYLDISRRPVPPAVVNLLPEVQARRYRALVIEDKGDAVVVAMSDPADLQALDNLEVL
CCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCHHHHCCCEEE
LAPKRLDLVVAPEAQLMQAFDNLYRRTEQIAQMAGQLEEEYAADEAFDLAALTAGDSDSE
EECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEEECCCCCCH
TTVVKLLQSIFEDAVQMRASDIHIEPGDKSLRIRQRVDGHLQETILNEVTIAAALVLRLK
HHHHHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEVKGHKIDVRISTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECCEEEEEEEEEEEECCHHHHHHHHHCCCCEEEECCCC
MPDAILQRVRKQIKRPHGMLLVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDHETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHCC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCQNCAVDYQLTSQDKAWLTSVSKG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCC
VDFSQARFRMGTGCQSCSGTGYRGRIGIFELLELDEAMVDAMRTGNPQAFAQAAYASPNF
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
TPLLISALNYLKQGMTTIEEVARLVEDVSETQIPLSQEMAQFDGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]