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The map label for this gene is leuA

Identifier: 119773476

GI number: 119773476

Start: 403271

End: 404839

Strand: Direct

Name: leuA

Synonym: Sama_0335

Alternate gene names: 119773476

Gene position: 403271-404839 (Clockwise)

Preceding gene: 119773474

Following gene: 119773477

Centisome position: 9.37

GC content: 54.75

Gene sequence:

>1569_bases
ATGTCAGACAGAGTCATCATTTTTGATACTACCCTGAGAGACGGTGAGCAGGCGCTGGCTGCGAGTCTTTCGGTAAAAGA
AAAATTGCAGGTGGCTCTGGCCCTTGAACGTTTGGGCGTGGATGTGATGGAAGTGGGCTTTCCCGTATCCTCTCCCGGTG
ATTTTGAATCAGTGCAAACCATTGCCCGCCATATTAAAAACAGTCGAGTGTGTGCTTTGTCGCGGGCGCTGGAAAAAGAC
ATAGACGCTGCCGCCCAGGCATTGTCTGTGGCTGAGCAGTTCCGAATTCACACCTTTATTTCCACCTCCAATATCCACGT
AGAGAGCAAGCTCAAGCGCTCATTTGATCAAGTGCTGGACATGGCCGTTGGTGCGGTGAAATACGCCCGCCGCTTTACCG
ATGACGTGGAATTCTCCTGTGAAGATGCGGGCCGCACGCCAATTGATAACCTGTGCCGCATGGTCGAAGCGGCCATTAGT
GCCGGTGCCCGCACCATCAATATTCCTGACACCGTTGGCTATACAGTACCCAGCGAATTTGGCGGCATCATCCAGACCCT
GTTCAATCGTGTGCCCAATATCGATCAGGCGGTGATCTCAGTGCACTGCCACGACGACCTGGGGCTGTCGGTCGCCAACT
CTATCGCCGCAGTGCAGGCCGGTGCTCGCCAGATTGAATGTACCGTGAACGGTATCGGCGAGCGCGCCGGTAACTGCTCG
TTGGAAGAAATCGCTATGATCTTGGCCACCCGCAAAGACATGCTGGGTCTGTATACCAATATCAATGCCCGTGAAATTCA
TCGCACCTCATCACTGGTCAGCCAGCTGTGCAACATGCCCATTCAGCCCAACAAGGCGATTGTCGGCTCCAATGCCTTTA
CCCACTCATCAGGTATTCACCAGGACGGCATGTTAAAGGCGCAAAACACCTATGAAATCATGACGCCAGAGAGTATTGGT
CTGAACCGTAACAACCTGAATATGACCTCGCGTTCCGGTCGTCACGTGATTAAACACCGCATGGAAGAAATGGGTTACGG
CGCCGGTGATTACGATATGGATACCCTGTACGCCGCCTTCCTGAAGTTGGCCGACAAAAAGGGCCAGGTATTCGACTACG
ATCTGGAAGCGTTGGCCTTTATGGAGGCGCAGGCCGAGGATGACGATCATTATGTGATGGAGCAATTGGTAGTGCAGTCA
GACTCTACCGAAGGCAAGGCTACCGCTACTGTGCGCATGCGCGTCGGGGACGAGGTCATCACCGAGGCCGCTACCGGTAA
TGGTCCGGTCGATGCCGCCTATAACGCCATCGCCCGCGCAACCCATCGCACCATTAATATCAGCAGCTACAAGCTTGGTG
CCAAAGGCGAGGGGCAAAATGCCCTGGGTCAGGTAGACATTACTGCCCGCTACGATGACCGTAACTTCCACGGCGTGGGA
TTGGCCACCGATGTAGTTGAGGCCTCGGCTCAGGCGCTGGTCCACGTCATGAACCTGACCTGGCGCGCCGACAAGGTCGC
CTATTGCAAGCAGCAGATGCAGCAAACCCGTGAGCTTGGCGGCGTTTAA

Upstream 100 bases:

>100_bases
CAAAGCCTTCACAAAACCCCGCGCCAACCACGCGGGGTTTTTTGTTTTCAAACACATGAGTTGTACAACGAGAACCGAAT
CGGTTCCATGGAGAATGGGA

Downstream 100 bases:

>100_bases
TGCCGTGCTTTTATAGCTTCATCCATTTATCAATGAATTCAATTTAAAAAGAGTGTCAGGGAGTAGAGCAAAGCATGAGT
TATCAGATAGCCGTATTGGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSDRVIIFDTTLRDGEQALAASLSVKEKLQVALALERLGVDVMEVGFPVSSPGDFESVQTIARHIKNSRVCALSRALEKD
IDAAAQALSVAEQFRIHTFISTSNIHVESKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAIS
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSIAAVQAGARQIECTVNGIGERAGNCS
LEEIAMILATRKDMLGLYTNINAREIHRTSSLVSQLCNMPIQPNKAIVGSNAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMEEMGYGAGDYDMDTLYAAFLKLADKKGQVFDYDLEALAFMEAQAEDDDHYVMEQLVVQS
DSTEGKATATVRMRVGDEVITEAATGNGPVDAAYNAIARATHRTINISSYKLGAKGEGQNALGQVDITARYDDRNFHGVG
LATDVVEASAQALVHVMNLTWRADKVAYCKQQMQQTRELGGV

Sequences:

>Translated_522_residues
MSDRVIIFDTTLRDGEQALAASLSVKEKLQVALALERLGVDVMEVGFPVSSPGDFESVQTIARHIKNSRVCALSRALEKD
IDAAAQALSVAEQFRIHTFISTSNIHVESKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAIS
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSIAAVQAGARQIECTVNGIGERAGNCS
LEEIAMILATRKDMLGLYTNINAREIHRTSSLVSQLCNMPIQPNKAIVGSNAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMEEMGYGAGDYDMDTLYAAFLKLADKKGQVFDYDLEALAFMEAQAEDDDHYVMEQLVVQS
DSTEGKATATVRMRVGDEVITEAATGNGPVDAAYNAIARATHRTINISSYKLGAKGEGQNALGQVDITARYDDRNFHGVG
LATDVVEASAQALVHVMNLTWRADKVAYCKQQMQQTRELGGV
>Mature_521_residues
SDRVIIFDTTLRDGEQALAASLSVKEKLQVALALERLGVDVMEVGFPVSSPGDFESVQTIARHIKNSRVCALSRALEKDI
DAAAQALSVAEQFRIHTFISTSNIHVESKLKRSFDQVLDMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAISA
GARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSIAAVQAGARQIECTVNGIGERAGNCSL
EEIAMILATRKDMLGLYTNINAREIHRTSSLVSQLCNMPIQPNKAIVGSNAFTHSSGIHQDGMLKAQNTYEIMTPESIGL
NRNNLNMTSRSGRHVIKHRMEEMGYGAGDYDMDTLYAAFLKLADKKGQVFDYDLEALAFMEAQAEDDDHYVMEQLVVQSD
STEGKATATVRMRVGDEVITEAATGNGPVDAAYNAIARATHRTINISSYKLGAKGEGQNALGQVDITARYDDRNFHGVGL
ATDVVEASAQALVHVMNLTWRADKVAYCKQQMQQTRELGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Homo sapiens, GI62198232, Length=296, Percent_Identity=23.3108108108108, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786261, Length=518, Percent_Identity=67.5675675675676, Blast_Score=723, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=537, Percent_Identity=25.8845437616387, Blast_Score=143, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=25.6505576208178, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=27.807486631016, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=27.9778393351801, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_SHEAM (A1S2E0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926216.1
- ProteinModelPortal:   A1S2E0
- SMR:   A1S2E0
- STRING:   A1S2E0
- GeneID:   4602591
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0335
- NMPDR:   fig|326297.7.peg.318
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   A1S2E0
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56858; Mature: 56727

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVIIFDTTLRDGEQALAASLSVKEKLQVALALERLGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKNSRVCALSRALEKDIDAAAQALSVAEQFRIHTFISTSNIHVESKLKRSFDQVLD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAISAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSIAAVQAGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILATRKDMLGLYTNINAREIHRTSSLVSQLCNMPIQPNKAIVGSNAFTHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYGAGDYDMDTLYAAF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFMEAQAEDDDHYVMEQLVVQSDSTEGKATATVRMRVGDEVI
HHHHCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHH
TEAATGNGPVDAAYNAIARATHRTINISSYKLGAKGEGQNALGQVDITARYDDRNFHGVG
HHHCCCCCCHHHHHHHHHHHHHCEEEECEEEECCCCCCCCCCEEEEEEEEECCCCCCCCC
LATDVVEASAQALVHVMNLTWRADKVAYCKQQMQQTRELGGV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDRVIIFDTTLRDGEQALAASLSVKEKLQVALALERLGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKNSRVCALSRALEKDIDAAAQALSVAEQFRIHTFISTSNIHVESKLKRSFDQVLD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEAAISAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSIAAVQAGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILATRKDMLGLYTNINAREIHRTSSLVSQLCNMPIQPNKAIVGSNAFTHSSGIH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMEEMGYGAGDYDMDTLYAAF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFMEAQAEDDDHYVMEQLVVQSDSTEGKATATVRMRVGDEVI
HHHHCCCCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHH
TEAATGNGPVDAAYNAIARATHRTINISSYKLGAKGEGQNALGQVDITARYDDRNFHGVG
HHHCCCCCCHHHHHHHHHHHHHCEEEECEEEECCCCCCCCCCEEEEEEEEECCCCCCCCC
LATDVVEASAQALVHVMNLTWRADKVAYCKQQMQQTRELGGV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA