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The map label for this gene is leuB [H]

Identifier: 119773477

GI number: 119773477

Start: 404914

End: 406008

Strand: Direct

Name: leuB [H]

Synonym: Sama_0336

Alternate gene names: 119773477

Gene position: 404914-406008 (Clockwise)

Preceding gene: 119773476

Following gene: 119773478

Centisome position: 9.4

GC content: 59.18

Gene sequence:

>1095_bases
ATGAGTTATCAGATAGCCGTATTGGCCGGTGACGGCATAGGCCCCGAGGTGATGGCAGAGGCACGTAAGGTGCTGGCAGA
AGTAGAGGCGCGCTTCGACCTCAATATCGAGTACAGCGAGTACGATGTGGGCGGCATTGCCATCGACAACCACGGCTGTC
CGCTGCCTGACGCTACCCTCAAGGGCTGTGAAGCGGCCGATGCCATTTTGTTTGGCTCTGTGGGTGGCCCCAAGTGGGAG
CATTTGCCTCCCAACGAGCAGCCAGAGCGCGGTGCGCTGCTGCCGCTGCGTGGTCACTTCGAGCTGTTCTGTAACCTGCG
TCCGGCCAGGCTGCACACCGGCCTTGAGCACATGTCGCCGCTGCGCGCGGATATCTCCGCCCAAGGTTTTGATGTGCTGT
GCGTGCGTGAGCTCACCGGCGGCATCTACTTCGGTAAGCCAAAAGGCCGTCAGGGCGAGGGCGAAGAGGAAGAAGCCTTC
GACACCATGCGTTACAGCCGCCGTGAAATCCGCCGTATTGCCAAAATCGCCTTCGAGGCGGCCCGCGGTCGTCGCGGCAA
GGTGACCTCGGTGGACAAAGCCAACGTACTGGCCTGCTCTGTGCTGTGGCGCGAGGTAGTGGAAGAGGTAGCCAAAGACT
TCCCCGATGTGACGCTTGAACACATCTATATCGATAACGCCACCATGCAGCTGCTGCGCCGTCCCTTCGATTTCGACGTG
ATGCTGTGCTCAAACCTGTTCGGTGATATTTTGTCGGACGAAATCGCCATGCTCACCGGCTCCATGGGGCTTTTGTCTTC
TGCGTCCATGAACGGTTCAGGCTTCGGCCTGTATGAACCAGCCGGTGGCAGTGCGCCGGATATCGCCGGCAAAGGCATCG
CCAACCCGATTGCGCAAATTCTCTCTGCTGCGCTGATGCTGCGTCACAGTCTTAAGCAGGAAGAGGCCGCCGCCTGTATC
GAGCGCGCCGTGGGTGCCGCTTTGGAAGCGGGCTTCTTAACCGGCGAGCTGCTGTCTGCTGACCAGCGTCACAATGCTAA
ATCAACCAAAGAAATGGGCGACTTTATCGCCAATGCAATCCGGGAAGGTGTGTAA

Upstream 100 bases:

>100_bases
AAACCCGTGAGCTTGGCGGCGTTTAATGCCGTGCTTTTATAGCTTCATCCATTTATCAATGAATTCAATTTAAAAAGAGT
GTCAGGGAGTAGAGCAAAGC

Downstream 100 bases:

>100_bases
TGGCAAAGACTCTGTATGAGAAAGTGTGGGACGCCCACCTGGTGGTTGAGCCAGCCGGTGAAGCGCCCATCATCTATGTA
GACCGGCATCTGGTGCACGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYQIAVLAGDGIGPEVMAEARKVLAEVEARFDLNIEYSEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
HLPPNEQPERGALLPLRGHFELFCNLRPARLHTGLEHMSPLRADISAQGFDVLCVRELTGGIYFGKPKGRQGEGEEEEAF
DTMRYSRREIRRIAKIAFEAARGRRGKVTSVDKANVLACSVLWREVVEEVAKDFPDVTLEHIYIDNATMQLLRRPFDFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNGSGFGLYEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAAACI
ERAVGAALEAGFLTGELLSADQRHNAKSTKEMGDFIANAIREGV

Sequences:

>Translated_364_residues
MSYQIAVLAGDGIGPEVMAEARKVLAEVEARFDLNIEYSEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
HLPPNEQPERGALLPLRGHFELFCNLRPARLHTGLEHMSPLRADISAQGFDVLCVRELTGGIYFGKPKGRQGEGEEEEAF
DTMRYSRREIRRIAKIAFEAARGRRGKVTSVDKANVLACSVLWREVVEEVAKDFPDVTLEHIYIDNATMQLLRRPFDFDV
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNGSGFGLYEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAAACI
ERAVGAALEAGFLTGELLSADQRHNAKSTKEMGDFIANAIREGV
>Mature_363_residues
SYQIAVLAGDGIGPEVMAEARKVLAEVEARFDLNIEYSEYDVGGIAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWEH
LPPNEQPERGALLPLRGHFELFCNLRPARLHTGLEHMSPLRADISAQGFDVLCVRELTGGIYFGKPKGRQGEGEEEEAFD
TMRYSRREIRRIAKIAFEAARGRRGKVTSVDKANVLACSVLWREVVEEVAKDFPDVTLEHIYIDNATMQLLRRPFDFDVM
LCSNLFGDILSDEIAMLTGSMGLLSSASMNGSGFGLYEPAGGSAPDIAGKGIANPIAQILSAALMLRHSLKQEEAAACIE
RAVGAALEAGFLTGELLSADQRHNAKSTKEMGDFIANAIREGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=352, Percent_Identity=31.8181818181818, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=29.5522388059701, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=29.7619047619048, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178816, Length=320, Percent_Identity=30, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=30, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178819, Length=148, Percent_Identity=39.8648648648649, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=69.6969696969697, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=36.1643835616438, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787381, Length=376, Percent_Identity=26.8617021276596, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.4561403508772, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=315, Percent_Identity=30.7936507936508, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=316, Percent_Identity=30.6962025316456, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=320, Percent_Identity=30, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=42.972972972973, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322097, Length=342, Percent_Identity=36.5497076023392, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324709, Length=334, Percent_Identity=32.0359281437126, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=27.7777777777778, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24643268, Length=344, Percent_Identity=34.593023255814, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24643270, Length=344, Percent_Identity=34.593023255814, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI161078635, Length=329, Percent_Identity=30.3951367781155, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078637, Length=329, Percent_Identity=30.3951367781155, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078633, Length=329, Percent_Identity=30.3951367781155, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24650122, Length=329, Percent_Identity=30.3951367781155, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078639, Length=325, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24661184, Length=333, Percent_Identity=29.1291291291291, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=25.6637168141593, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=25.6637168141593, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20130355, Length=331, Percent_Identity=25.0755287009063, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39365; Mature: 39234

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQIAVLAGDGIGPEVMAEARKVLAEVEARFDLNIEYSEYDVGGIAIDNHGCPLPDATL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEHLPPNEQPERGALLPLRGHFELFCNLRPARLHTGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRADISAQGFDVLCVRELTGGIYFGKPKGRQGEGEEEEAFDTMRYSRREIRRIAKIAFEA
HHHCCCCCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRGKVTSVDKANVLACSVLWREVVEEVAKDFPDVTLEHIYIDNATMQLLRRPFDFDV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCCHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNGSGFGLYEPAGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
LSAALMLRHSLKQEEAAACIERAVGAALEAGFLTGELLSADQRHNAKSTKEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
REGV
HHCC
>Mature Secondary Structure 
SYQIAVLAGDGIGPEVMAEARKVLAEVEARFDLNIEYSEYDVGGIAIDNHGCPLPDATL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEHLPPNEQPERGALLPLRGHFELFCNLRPARLHTGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRADISAQGFDVLCVRELTGGIYFGKPKGRQGEGEEEEAFDTMRYSRREIRRIAKIAFEA
HHHCCCCCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARGRRGKVTSVDKANVLACSVLWREVVEEVAKDFPDVTLEHIYIDNATMQLLRRPFDFDV
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCCCHH
MLCSNLFGDILSDEIAMLTGSMGLLSSASMNGSGFGLYEPAGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
LSAALMLRHSLKQEEAAACIERAVGAALEAGFLTGELLSADQRHNAKSTKEMGDFIANAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
REGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]