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The map label for this gene is rnhB

Identifier: 119717477

GI number: 119717477

Start: 3455003

End: 3455725

Strand: Reverse

Name: rnhB

Synonym: Noca_3253

Alternate gene names: 119717477

Gene position: 3455725-3455003 (Counterclockwise)

Preceding gene: 119717478

Following gene: 119717476

Centisome position: 69.31

GC content: 72.89

Gene sequence:

>723_bases
ATGAGCGAGCTGCCCCGCGGATCCACGATCCGCAAGGACGCCGGCCTGTACGGCTACGAGCGGGCCCTGCGCCGGCACGG
GATCGAGCCGATCGCCGGCGTCGACGAGGCCGGCCGTGGCGCCTGCGCCGGGCCGCTGGTCGCCGGCGCCGCCATCCTGC
CTCCCGGCAAGGCCGGCATCGTCCCCGGCCTCGCGGACTCCAAGCTGCTCACCGAGCGGGCGCGGGAGCGTTGCTACGAG
CAGATCGTGCGCCGGGCGGTCTCCTGGTCGGTCGTCGTGGTCTCGCACGAGGAGTGCGACCGGCTCGGCATGCACGTGGC
CAACGTCGAGGCCCTGCGGCGGGCGGTGGCGCTGCTCGACCTGCCGCCGGCGTACGTCCTGACCGACGGGTTCCCGGTCG
ACGGCCTCGGTGTCCCCGGCCTCGCGGTGTGGAAGGGCGACCGGGTGGCCGCGTGCATCTCCGCGGCCTCGGTCCTCGCG
AAGGTCACCCGGGACCGGATCATGACCGAGCTCGACGCGTCCTGGCCGGCGTACGACTTCAAGACCCACAAGGGCTACAT
CACCGAGGTGCACAGCGCCGCGCTCACCGCGCACGGACCCTCGCCGATCCACCGGATGCGCTTCGTCAACGTCCGGCGGG
CCGCGGGGCTCGAGCCGCTGGATCCCGTGCAGAATGGTGAGGAAGCGGTCGAGCTGGTCGAGGTCACGGAGGGAACGAGA
TGA

Upstream 100 bases:

>100_bases
CGTGCTGCTCTGGCCCAGTGATCACTTCAAGTTCCTGCATCGTCCCGACACCTTCGCGGGCGTGCCCGACCCGTCCTAGC
GCCGTGACCGAGCCAGCGCC

Downstream 100 bases:

>100_bases
GCGCCGAGGATCTCGAGAAGTACGAGACCGAGATGGAGCTGACCCTCTACCGCGAGTACCGCGACGTGGTCGGCATCTTC
AAGTACGTCGTCGAGACCGA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSELPRGSTIRKDAGLYGYERALRRHGIEPIAGVDEAGRGACAGPLVAGAAILPPGKAGIVPGLADSKLLTERARERCYE
QIVRRAVSWSVVVVSHEECDRLGMHVANVEALRRAVALLDLPPAYVLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLA
KVTRDRIMTELDASWPAYDFKTHKGYITEVHSAALTAHGPSPIHRMRFVNVRRAAGLEPLDPVQNGEEAVELVEVTEGTR

Sequences:

>Translated_240_residues
MSELPRGSTIRKDAGLYGYERALRRHGIEPIAGVDEAGRGACAGPLVAGAAILPPGKAGIVPGLADSKLLTERARERCYE
QIVRRAVSWSVVVVSHEECDRLGMHVANVEALRRAVALLDLPPAYVLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLA
KVTRDRIMTELDASWPAYDFKTHKGYITEVHSAALTAHGPSPIHRMRFVNVRRAAGLEPLDPVQNGEEAVELVEVTEGTR
>Mature_239_residues
SELPRGSTIRKDAGLYGYERALRRHGIEPIAGVDEAGRGACAGPLVAGAAILPPGKAGIVPGLADSKLLTERARERCYEQ
IVRRAVSWSVVVVSHEECDRLGMHVANVEALRRAVALLDLPPAYVLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLAK
VTRDRIMTELDASWPAYDFKTHKGYITEVHSAALTAHGPSPIHRMRFVNVRRAAGLEPLDPVQNGEEAVELVEVTEGTR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=187, Percent_Identity=46.524064171123, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_NOCSJ (A1SLS0)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924442.1
- ProteinModelPortal:   A1SLS0
- SMR:   A1SLS0
- STRING:   A1SLS0
- GeneID:   4599115
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3253
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   NSP35761:NOCA_3253-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25743; Mature: 25612

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPRGSTIRKDAGLYGYERALRRHGIEPIAGVDEAGRGACAGPLVAGAAILPPGKAGI
CCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCC
VPGLADSKLLTERARERCYEQIVRRAVSWSVVVVSHEECDRLGMHVANVEALRRAVALLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHC
LPPAYVLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLAKVTRDRIMTELDASWPAYDF
CCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KTHKGYITEVHSAALTAHGPSPIHRMRFVNVRRAAGLEPLDPVQNGEEAVELVEVTEGTR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELPRGSTIRKDAGLYGYERALRRHGIEPIAGVDEAGRGACAGPLVAGAAILPPGKAGI
CCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCC
VPGLADSKLLTERARERCYEQIVRRAVSWSVVVVSHEECDRLGMHVANVEALRRAVALLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHC
LPPAYVLTDGFPVDGLGVPGLAVWKGDRVAACISAASVLAKVTRDRIMTELDASWPAYDF
CCCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KTHKGYITEVHSAALTAHGPSPIHRMRFVNVRRAAGLEPLDPVQNGEEAVELVEVTEGTR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA