Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 119717412

GI number: 119717412

Start: 3387004

End: 3387873

Strand: Reverse

Name: truB [H]

Synonym: Noca_3188

Alternate gene names: 119717412

Gene position: 3387873-3387004 (Counterclockwise)

Preceding gene: 119717413

Following gene: 119717411

Centisome position: 67.95

GC content: 75.29

Gene sequence:

>870_bases
ATGACGACGAGTGAGGCCGGGCTGGTCGTCGTCGACAAGCCCGGCGGCCTGACCTCGCACGACGTCGTCTCGCGGGTGCG
GCGCCTGACCGGCACCCGCAAGGTCGGGCACGCGGGCACCCTCGACCCGATGGCCACCGGGGTCCTCGTGCTCGGGATCA
ACCGGGCGACCCGGCTGCTCGGGCACCTGATGCTGACCGAGAAGGCGTACGACGCCACCATCCGGCTCGGTGTCGCCACG
AGCACCGACGACGCCGAGGGCGAGGTGACCGCGACCGCGTCGGCCGCGGGCCTGGACGAGGCGACCGTCCGAGCGGCGGC
CGCCGAGTTCGTGGGCGACCTGCTGCAGGTGCCGACCGCGGTGTCGGCGATCAAGGTCGACGGCAAGCGCGCCTACCAGC
GGGTCCGGGCGGGCGAGGAGGTCGCGCTGGAGGCCCGCCCGGTCACGGTCCACGAACTGGTGGTCCACGAGGTCCGACCG
GCCGGCGACACCTGCGACCTCGACGTCGCGGTGCGCTGCTCGAGCGGCACCTACGTGCGCGCGATCGCCCGCGACCTCGG
CGCCCGCCTCGGCGTGGGCGGCCACCTGACGGCGCTGCGTCGTACCGCCGTCGGCCCCTACGGGCTGGACGTGGCGCACA
CCCTGGACGAACTCGGCGAGGAGCTCGTGGTGCTGCCGGTCGCGGCGGCTGCGCGGGCGGCGTTCGCGAGCCGCGACCTC
GATGACGCCCAGGCCACCGACGTCCGCTTCGGGCGGGCGCTGGACCTCGACCTCCCGGGCCTGACCGCCGTGTTCGCCCC
GGACGGGGAGTTCCTCGCGCTCTACGAGCCGCGCGGCGGCGCGGCCAAGGCCGTCGCGGTCTTCGTGTGA

Upstream 100 bases:

>100_bases
ACGGCGTACGCCGGCGAGGCCGACCCCTACAAGAAGCCGCGTCCGGCCGACGCCGACGAGCCCGACGACTCCGGCGAACC
CGGCGAGTTCGACGGGGTCG

Downstream 100 bases:

>100_bases
GCGCTCGGTAGGCTTCCCCGGTGCAGATCTGGCGCTCCCTGGAGGAGGTCCCCTCCGGCCTCGGGCCGACCGCCGTGGTG
ATCGGCAACTTCGACGGGGT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTTSEAGLVVVDKPGGLTSHDVVSRVRRLTGTRKVGHAGTLDPMATGVLVLGINRATRLLGHLMLTEKAYDATIRLGVAT
STDDAEGEVTATASAAGLDEATVRAAAAEFVGDLLQVPTAVSAIKVDGKRAYQRVRAGEEVALEARPVTVHELVVHEVRP
AGDTCDLDVAVRCSSGTYVRAIARDLGARLGVGGHLTALRRTAVGPYGLDVAHTLDELGEELVVLPVAAAARAAFASRDL
DDAQATDVRFGRALDLDLPGLTAVFAPDGEFLALYEPRGGAAKAVAVFV

Sequences:

>Translated_289_residues
MTTSEAGLVVVDKPGGLTSHDVVSRVRRLTGTRKVGHAGTLDPMATGVLVLGINRATRLLGHLMLTEKAYDATIRLGVAT
STDDAEGEVTATASAAGLDEATVRAAAAEFVGDLLQVPTAVSAIKVDGKRAYQRVRAGEEVALEARPVTVHELVVHEVRP
AGDTCDLDVAVRCSSGTYVRAIARDLGARLGVGGHLTALRRTAVGPYGLDVAHTLDELGEELVVLPVAAAARAAFASRDL
DDAQATDVRFGRALDLDLPGLTAVFAPDGEFLALYEPRGGAAKAVAVFV
>Mature_288_residues
TTSEAGLVVVDKPGGLTSHDVVSRVRRLTGTRKVGHAGTLDPMATGVLVLGINRATRLLGHLMLTEKAYDATIRLGVATS
TDDAEGEVTATASAAGLDEATVRAAAAEFVGDLLQVPTAVSAIKVDGKRAYQRVRAGEEVALEARPVTVHELVVHEVRPA
GDTCDLDVAVRCSSGTYVRAIARDLGARLGVGGHLTALRRTAVGPYGLDVAHTLDELGEELVVLPVAAAARAAFASRDLD
DAQATDVRFGRALDLDLPGLTAVFAPDGEFLALYEPRGGAAKAVAVFV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=231, Percent_Identity=34.6320346320346, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI4503337, Length=199, Percent_Identity=33.6683417085427, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI215599015, Length=199, Percent_Identity=33.6683417085427, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367200, Length=214, Percent_Identity=39.2523364485981, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17553978, Length=200, Percent_Identity=32.5, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=31.2169312169312, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=201, Percent_Identity=32.3383084577114, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281364189, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364187, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364185, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364183, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI62471759, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17975520, Length=201, Percent_Identity=32.3383084577114, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 29993; Mature: 29862

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSEAGLVVVDKPGGLTSHDVVSRVRRLTGTRKVGHAGTLDPMATGVLVLGINRATRLL
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCHHHHHH
GHLMLTEKAYDATIRLGVATSTDDAEGEVTATASAAGLDEATVRAAAAEFVGDLLQVPTA
HHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCH
VSAIKVDGKRAYQRVRAGEEVALEARPVTVHELVVHEVRPAGDTCDLDVAVRCSSGTYVR
HHEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
AIARDLGARLGVGGHLTALRRTAVGPYGLDVAHTLDELGEELVVLPVAAAARAAFASRDL
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCC
DDAQATDVRFGRALDLDLPGLTAVFAPDGEFLALYEPRGGAAKAVAVFV
CCCHHHHEECCCEEECCCCCCEEEECCCCCEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure 
TTSEAGLVVVDKPGGLTSHDVVSRVRRLTGTRKVGHAGTLDPMATGVLVLGINRATRLL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCHHHHHH
GHLMLTEKAYDATIRLGVATSTDDAEGEVTATASAAGLDEATVRAAAAEFVGDLLQVPTA
HHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCH
VSAIKVDGKRAYQRVRAGEEVALEARPVTVHELVVHEVRPAGDTCDLDVAVRCSSGTYVR
HHEEEECHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHH
AIARDLGARLGVGGHLTALRRTAVGPYGLDVAHTLDELGEELVVLPVAAAARAAFASRDL
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCC
DDAQATDVRFGRALDLDLPGLTAVFAPDGEFLALYEPRGGAAKAVAVFV
CCCHHHHEECCCEEECCCCCCEEEECCCCCEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA