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The map label for this gene is bfmBC [H]

Identifier: 119716531

GI number: 119716531

Start: 2454025

End: 2455404

Strand: Reverse

Name: bfmBC [H]

Synonym: Noca_2304

Alternate gene names: 119716531

Gene position: 2455404-2454025 (Counterclockwise)

Preceding gene: 119716533

Following gene: 119716530

Centisome position: 49.25

GC content: 71.59

Gene sequence:

>1380_bases
GTGGCCGACGCCGACTTCGACGTACTCATCCTCGGTGCGGGCTCCGGCGGTTACGCCTGCGCCCTGCGCTCAGCACAGCT
GGGCCTGTCCGTGGGCCTGGTGGAGAAGTCGCACCTGGGCGGCACCTGCCTGCACATCGGCTGCATCCCGACCAAGGCGC
TGCTGCACGCCGCCGAGGTCGCGGACCAGGCCCGCGAGGCCGAGACGTTCGGAGTGCGCGCCACCCTGGAGGGCATCGAC
ATGGCGGGGGTCAACGCCTACAAGGACGGTGTCGTCTCGCGGCTGTTCAAGGGCCTCACCGGCCTGATCAAGGGCCGCGG
CATCACGGTGATCGAGGGCGCCGGCCGGCTCACCGGCCCGCGGACGGTCACCGTCGGCGAGACGGCGTACACCGGCAAGC
ACGTGGTGCTCGCGTCCGGCTCGTACGCCCGCACCCTGCCCGGGCTCGAGATCGACGGCGAGCGGGTGATCACCTCCGAC
CACGCCTTGCGGCTGGATCGGGTGCCCGCCTCGGTCGTGGTGCTCGGCGGCGGCGTCATCGGCTGCGAGTTCGCCAGCGT
GTGGCGCAGCTTCGGTGCCGACGTGACCATCGTCGAGGCGCTGCCGCGGCTGGTGGCCGTCGAGGACGAGGCGTCCTCCA
AGGCGCTGGAGCGGGCGTTCCGCAAGCGCGGCATCGTGTTCCGCACCGGCACCCCGTTCCAGAGCGTCAAGCACACCGAC
ACCGGCGTCGCGGTGACCGTCGAGGGGGGCGACGTGATCGAGGCCGACCTGCTGCTTGTCGCAGTCGGTCGCGGGCCGGT
CACCGAAGGCCTCGGCTACGACGAGCAGGGCGTGACCATGGACCGCGGGTTCGTGCTCACCGACGAGCGGTGCCGCACCA
ACCTGGACGGCGTGTACGCCGTCGGCGACATCGTCCCCGGCCCGCAGCTGGCGCACCGCGGCTTCCAGCAGGGCATCTTC
GTCGCCGAGGACATCGCGGGCCTCGACCCGAGCCCGATCGACGAGGCCGGCATCCCCCGCGTGACGTACTCGCACCCGGA
GGTCGCCTCCGTCGGGCTCGACGAGGCGCGTGCGGCGGAGACCTACGGCGCCGACGGCATCGAGACGCTCACCTACGACC
TGGGCGGCAACGGCAAGAGCCAGATCCTCAAGACCCAGGGCTTCGTGAAGCTGATCCGTCGCAAGGACGGCCCCGTCGTG
GGCGTGCACATGGTCGGCGACCGGGTCGGCGAGCTCATCGGCGAGGCCCAGATGATCTACAACTGGGAGGGGTACCCGGA
GGACGTGGCACCCCTTGTCCACGCCCACCCGACGCAGAACGAGGCGCTCGGCGAGGCGCACCTGGCGCTGGCGGGCAAAC
CCCTGCACGCCCACTCCTGA

Upstream 100 bases:

>100_bases
CAAGGTGCGCCGCCAGGTTCGCCGCGGTGACCACGAGGTGACCCGCGAGTAGCCCGGAGGCCGGTGTGGACACGTTGCGC
GGAAGTGCAAGGATGGGCGG

Downstream 100 bases:

>100_bases
ACCCGAACACCAGCACCTGAAGCGAAGGAAGTCCATTGGCCACCGAAGTCAACCTGCCGGCACTCGGCGAGTCCGTCACC
GAAGGCACCGTCACCCGCTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MADADFDVLILGAGSGGYACALRSAQLGLSVGLVEKSHLGGTCLHIGCIPTKALLHAAEVADQAREAETFGVRATLEGID
MAGVNAYKDGVVSRLFKGLTGLIKGRGITVIEGAGRLTGPRTVTVGETAYTGKHVVLASGSYARTLPGLEIDGERVITSD
HALRLDRVPASVVVLGGGVIGCEFASVWRSFGADVTIVEALPRLVAVEDEASSKALERAFRKRGIVFRTGTPFQSVKHTD
TGVAVTVEGGDVIEADLLLVAVGRGPVTEGLGYDEQGVTMDRGFVLTDERCRTNLDGVYAVGDIVPGPQLAHRGFQQGIF
VAEDIAGLDPSPIDEAGIPRVTYSHPEVASVGLDEARAAETYGADGIETLTYDLGGNGKSQILKTQGFVKLIRRKDGPVV
GVHMVGDRVGELIGEAQMIYNWEGYPEDVAPLVHAHPTQNEALGEAHLALAGKPLHAHS

Sequences:

>Translated_459_residues
MADADFDVLILGAGSGGYACALRSAQLGLSVGLVEKSHLGGTCLHIGCIPTKALLHAAEVADQAREAETFGVRATLEGID
MAGVNAYKDGVVSRLFKGLTGLIKGRGITVIEGAGRLTGPRTVTVGETAYTGKHVVLASGSYARTLPGLEIDGERVITSD
HALRLDRVPASVVVLGGGVIGCEFASVWRSFGADVTIVEALPRLVAVEDEASSKALERAFRKRGIVFRTGTPFQSVKHTD
TGVAVTVEGGDVIEADLLLVAVGRGPVTEGLGYDEQGVTMDRGFVLTDERCRTNLDGVYAVGDIVPGPQLAHRGFQQGIF
VAEDIAGLDPSPIDEAGIPRVTYSHPEVASVGLDEARAAETYGADGIETLTYDLGGNGKSQILKTQGFVKLIRRKDGPVV
GVHMVGDRVGELIGEAQMIYNWEGYPEDVAPLVHAHPTQNEALGEAHLALAGKPLHAHS
>Mature_458_residues
ADADFDVLILGAGSGGYACALRSAQLGLSVGLVEKSHLGGTCLHIGCIPTKALLHAAEVADQAREAETFGVRATLEGIDM
AGVNAYKDGVVSRLFKGLTGLIKGRGITVIEGAGRLTGPRTVTVGETAYTGKHVVLASGSYARTLPGLEIDGERVITSDH
ALRLDRVPASVVVLGGGVIGCEFASVWRSFGADVTIVEALPRLVAVEDEASSKALERAFRKRGIVFRTGTPFQSVKHTDT
GVAVTVEGGDVIEADLLLVAVGRGPVTEGLGYDEQGVTMDRGFVLTDERCRTNLDGVYAVGDIVPGPQLAHRGFQQGIFV
AEDIAGLDPSPIDEAGIPRVTYSHPEVASVGLDEARAAETYGADGIETLTYDLGGNGKSQILKTQGFVKLIRRKDGPVVG
VHMVGDRVGELIGEAQMIYNWEGYPEDVAPLVHAHPTQNEALGEAHLALAGKPLHAHS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=36.1522198731501, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=28.755364806867, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI291045266, Length=446, Percent_Identity=28.0269058295964, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI22035672, Length=470, Percent_Identity=27.4468085106383, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148277065, Length=400, Percent_Identity=25.75, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI33519430, Length=400, Percent_Identity=25.75, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI33519428, Length=400, Percent_Identity=25.75, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI33519426, Length=400, Percent_Identity=25.75, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI148277071, Length=400, Percent_Identity=25.75, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI291045268, Length=356, Percent_Identity=28.6516853932584, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=35.920177383592, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=27.1929824561404, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=27.0207852193995, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=27.7533039647577, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=36.5384615384615, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71983429, Length=378, Percent_Identity=30.4232804232804, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=378, Percent_Identity=30.4232804232804, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=25.8196721311475, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=24.5901639344262, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=35.2818371607516, Blast_Score=242, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=27.9411764705882, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=26.3948497854077, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=36.6737739872068, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24640549, Length=441, Percent_Identity=27.891156462585, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640553, Length=441, Percent_Identity=27.891156462585, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640551, Length=441, Percent_Identity=27.891156462585, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI17737741, Length=450, Percent_Identity=25.5555555555556, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48097; Mature: 47966

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADADFDVLILGAGSGGYACALRSAQLGLSVGLVEKSHLGGTCLHIGCIPTKALLHAAEV
CCCCCEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHH
ADQAREAETFGVRATLEGIDMAGVNAYKDGVVSRLFKGLTGLIKGRGITVIEGAGRLTGP
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
RTVTVGETAYTGKHVVLASGSYARTLPGLEIDGERVITSDHALRLDRVPASVVVLGGGVI
EEEEECCCEECCCEEEEECCCCHHCCCCCEECCCEEEECCCCEEEECCCEEEEEECCCEE
GCEFASVWRSFGADVTIVEALPRLVAVEDEASSKALERAFRKRGIVFRTGTPFQSVKHTD
EHHHHHHHHHHCCCEEHHHHHHHHEEECCCHHHHHHHHHHHHCCEEEECCCCHHHCCCCC
TGVAVTVEGGDVIEADLLLVAVGRGPVTEGLGYDEQGVTMDRGFVLTDERCRTNLDGVYA
CCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEECHHHHCCCCCEEE
VGDIVPGPQLAHRGFQQGIFVAEDIAGLDPSPIDEAGIPRVTYSHPEVASVGLDEARAAE
ECCCCCCHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHH
TYGADGIETLTYDLGGNGKSQILKTQGFVKLIRRKDGPVVGVHMVGDRVGELIGEAQMIY
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCEEEE
NWEGYPEDVAPLVHAHPTQNEALGEAHLALAGKPLHAHS
ECCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
ADADFDVLILGAGSGGYACALRSAQLGLSVGLVEKSHLGGTCLHIGCIPTKALLHAAEV
CCCCEEEEEEECCCCCEEEEEEHHHCCEEEEEEECCCCCCEEEEEECCCHHHHHHHHHH
ADQAREAETFGVRATLEGIDMAGVNAYKDGVVSRLFKGLTGLIKGRGITVIEGAGRLTGP
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
RTVTVGETAYTGKHVVLASGSYARTLPGLEIDGERVITSDHALRLDRVPASVVVLGGGVI
EEEEECCCEECCCEEEEECCCCHHCCCCCEECCCEEEECCCCEEEECCCEEEEEECCCEE
GCEFASVWRSFGADVTIVEALPRLVAVEDEASSKALERAFRKRGIVFRTGTPFQSVKHTD
EHHHHHHHHHHCCCEEHHHHHHHHEEECCCHHHHHHHHHHHHCCEEEECCCCHHHCCCCC
TGVAVTVEGGDVIEADLLLVAVGRGPVTEGLGYDEQGVTMDRGFVLTDERCRTNLDGVYA
CCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEECHHHHCCCCCEEE
VGDIVPGPQLAHRGFQQGIFVAEDIAGLDPSPIDEAGIPRVTYSHPEVASVGLDEARAAE
ECCCCCCHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHH
TYGADGIETLTYDLGGNGKSQILKTQGFVKLIRRKDGPVVGVHMVGDRVGELIGEAQMIY
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCEEEE
NWEGYPEDVAPLVHAHPTQNEALGEAHLALAGKPLHAHS
ECCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]