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The map label for this gene is sucB [H]

Identifier: 119716530

GI number: 119716530

Start: 2452196

End: 2453989

Strand: Reverse

Name: sucB [H]

Synonym: Noca_2303

Alternate gene names: 119716530

Gene position: 2453989-2452196 (Counterclockwise)

Preceding gene: 119716531

Following gene: 119716529

Centisome position: 49.22

GC content: 73.02

Gene sequence:

>1794_bases
TTGGCCACCGAAGTCAACCTGCCGGCACTCGGCGAGTCCGTCACCGAAGGCACCGTCACCCGCTGGCTCAAGCAGGTGGG
CGACTCGGTGGCCGTCGACGAGCCGCTGCTCGAGGTCTCCACGGACAAGGTCGACACCGAGATCCCCTCCCCCATCGCCG
GCACGCTGCTGGAGATCCGGGCCAACGAGGACGACACCGTCGAGGTCGGTGCCGTGCTGGCGGTGATCGGGGACGCCGGT
GAGTCTTCGGGTGACTCCGGCGGCGCCCAGGCCGAGGCCCAGCCCGACCCCGAGCCGGAGCCCGAGGCACAGCCCGAGCC
GGAGCCCGCCCAGGAGCAGGCGCAGCAGGAGGCGCAGCAGCCCGCTGCCGAGCAGCCGGCCCCGGCGGCCGAGGCCAAGC
CGTCCGGTGGCTCCGGGACGGCCGTGACCCTCCCGGCCCTCGGCGAGTCGGTCACCGAGGGCACCGTCACCCGCTGGCTG
AAGTCGGTCGGCGACGAGGTCGCCGTGGACGAGCCGCTGCTCGAGGTCTCCACCGACAAGGTCGACACCGAGATCCCCTC
GCCGGTCGCCGGCACCCTGCTCGAGATCAAGGTCGCCGAGGACGAGACCGTCGAGGTCGGGGCCGAGCTCGCCGTGATCG
GGTCCGGACAGGCCGCGCCGGCCGAGTCGAAGCCCGAGCCGACCCCCGAGCCCGAGCCGGAGCCGACCCCCGAGCCCGAG
CCCGAGCCCGAGGCGGCACCCGAGCCCGAGCCGGCACCCGCGCCCCAGGAGCAGGCCGCCCCGCCGGCTCCCGCGCCGGC
CGCCGCCGCCCAGCCCACTGCGCAGCCCGCCGCCCAGCCCGTCGCGAGCGGTGACGGCCCGGGCTACGTGACGCCGCTGG
TGCGCAAGATGGCCGCCCAGCACGGCGTGGACCTCGCGAGCGTCACCGGCACCGGCGTGGGGGGGAGGATCCGCAAGCAG
GACGTGCTCGACGCCGCCAAGGCCCAGCAACAGGCCCAGCAACAGGCCCAGCAGCCCGCCCAGGCGGCTCCGGCCGCCCC
GGCTGCCCCCTCGGCACCGGTCTCCTCGGGCGCGCCGTCGCCGCTGCGCGGCACCACGGTGAAGATGTCGCGGCTGCGCA
AGATCATCGCCACGCGCATGGTCGACTCGCTGCAGACCTCCGCCCAGCTGACCCAGGTGGTCGAGGTCGACGTCACCAAC
ATCGCGCGGCTGCGCGACGAGGCGAAGGCGGACTTCCTGTCCCGGGAGGGCGTCAAGCTGTCGTACCTGCCGTTCTTCGC
GAAGGCCGCGATCGACGCGCTCAAGCACCACCCGGCGCTGAACGCGACCATCGACACCGAGGCGGGCGAGGTCACCTACT
ACGACCGCGAGAACCTCGCGATCGCGGTGGACACCGAGAAGGGTCTGATCACCCCGGTGATCAAGGACGCGGGCGACCTC
TCCATCGCCGGCCTGGCGAAGAAGATCGCCGACGTCGCCCAGCGCACGCGCACCAACAAGATCGGCCCGGACGAGCTCTC
GGGCGGGACGTTCACGATCACCAACCTGGGCAGCGTCGGGGCCCTGTGGGACACCCCGATCGTGAACAAGCCGCAGGTCG
CGATCCTCGGGCCCGGCGCCGTCGTCAAGCGGCCCGTGGTCATCGACGACCCGAACCTCGGCGAGACGATCGCGGTGCGC
TACATGGTCTACCTGGCACTCACCTACGACCACCAGCTGGTCGACGGCGCCGACGCAGGCCGTTTCCTCACCGACGTGAA
GCAGCGCCTGGAGTCGGGCCAGTTCGAGGTCTGA

Upstream 100 bases:

>100_bases
AGAACGAGGCGCTCGGCGAGGCGCACCTGGCGCTGGCGGGCAAACCCCTGCACGCCCACTCCTGAACCCGAACACCAGCA
CCTGAAGCGAAGGAAGTCCA

Downstream 100 bases:

>100_bases
GGAGAGCATGTCGCTGACCGTCGTCATCGCCGGCGCCTCCGGCTTCCTCGGCACCCACCTGAGCCGGGAGCTGACCGAGC
GCGGCCACGACGTGGTCGCG

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MATEVNLPALGESVTEGTVTRWLKQVGDSVAVDEPLLEVSTDKVDTEIPSPIAGTLLEIRANEDDTVEVGAVLAVIGDAG
ESSGDSGGAQAEAQPDPEPEPEAQPEPEPAQEQAQQEAQQPAAEQPAPAAEAKPSGGSGTAVTLPALGESVTEGTVTRWL
KSVGDEVAVDEPLLEVSTDKVDTEIPSPVAGTLLEIKVAEDETVEVGAELAVIGSGQAAPAESKPEPTPEPEPEPTPEPE
PEPEAAPEPEPAPAPQEQAAPPAPAPAAAAQPTAQPAAQPVASGDGPGYVTPLVRKMAAQHGVDLASVTGTGVGGRIRKQ
DVLDAAKAQQQAQQQAQQPAQAAPAAPAAPSAPVSSGAPSPLRGTTVKMSRLRKIIATRMVDSLQTSAQLTQVVEVDVTN
IARLRDEAKADFLSREGVKLSYLPFFAKAAIDALKHHPALNATIDTEAGEVTYYDRENLAIAVDTEKGLITPVIKDAGDL
SIAGLAKKIADVAQRTRTNKIGPDELSGGTFTITNLGSVGALWDTPIVNKPQVAILGPGAVVKRPVVIDDPNLGETIAVR
YMVYLALTYDHQLVDGADAGRFLTDVKQRLESGQFEV

Sequences:

>Translated_597_residues
MATEVNLPALGESVTEGTVTRWLKQVGDSVAVDEPLLEVSTDKVDTEIPSPIAGTLLEIRANEDDTVEVGAVLAVIGDAG
ESSGDSGGAQAEAQPDPEPEPEAQPEPEPAQEQAQQEAQQPAAEQPAPAAEAKPSGGSGTAVTLPALGESVTEGTVTRWL
KSVGDEVAVDEPLLEVSTDKVDTEIPSPVAGTLLEIKVAEDETVEVGAELAVIGSGQAAPAESKPEPTPEPEPEPTPEPE
PEPEAAPEPEPAPAPQEQAAPPAPAPAAAAQPTAQPAAQPVASGDGPGYVTPLVRKMAAQHGVDLASVTGTGVGGRIRKQ
DVLDAAKAQQQAQQQAQQPAQAAPAAPAAPSAPVSSGAPSPLRGTTVKMSRLRKIIATRMVDSLQTSAQLTQVVEVDVTN
IARLRDEAKADFLSREGVKLSYLPFFAKAAIDALKHHPALNATIDTEAGEVTYYDRENLAIAVDTEKGLITPVIKDAGDL
SIAGLAKKIADVAQRTRTNKIGPDELSGGTFTITNLGSVGALWDTPIVNKPQVAILGPGAVVKRPVVIDDPNLGETIAVR
YMVYLALTYDHQLVDGADAGRFLTDVKQRLESGQFEV
>Mature_596_residues
ATEVNLPALGESVTEGTVTRWLKQVGDSVAVDEPLLEVSTDKVDTEIPSPIAGTLLEIRANEDDTVEVGAVLAVIGDAGE
SSGDSGGAQAEAQPDPEPEPEAQPEPEPAQEQAQQEAQQPAAEQPAPAAEAKPSGGSGTAVTLPALGESVTEGTVTRWLK
SVGDEVAVDEPLLEVSTDKVDTEIPSPVAGTLLEIKVAEDETVEVGAELAVIGSGQAAPAESKPEPTPEPEPEPTPEPEP
EPEAAPEPEPAPAPQEQAAPPAPAPAAAAQPTAQPAAQPVASGDGPGYVTPLVRKMAAQHGVDLASVTGTGVGGRIRKQD
VLDAAKAQQQAQQQAQQPAQAAPAAPAAPSAPVSSGAPSPLRGTTVKMSRLRKIIATRMVDSLQTSAQLTQVVEVDVTNI
ARLRDEAKADFLSREGVKLSYLPFFAKAAIDALKHHPALNATIDTEAGEVTYYDRENLAIAVDTEKGLITPVIKDAGDLS
IAGLAKKIADVAQRTRTNKIGPDELSGGTFTITNLGSVGALWDTPIVNKPQVAILGPGAVVKRPVVIDDPNLGETIAVRY
MVYLALTYDHQLVDGADAGRFLTDVKQRLESGQFEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=40.1785714285714, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI31711992, Length=594, Percent_Identity=29.6296296296296, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI110671329, Length=491, Percent_Identity=26.8839103869654, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI203098816, Length=482, Percent_Identity=26.5560165975104, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI203098753, Length=477, Percent_Identity=26.6247379454927, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=33.125, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786946, Length=454, Percent_Identity=33.7004405286344, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786305, Length=457, Percent_Identity=28.4463894967177, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=453, Percent_Identity=32.2295805739514, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=29.4247787610619, Blast_Score=160, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=456, Percent_Identity=31.359649122807, Blast_Score=155, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=27.5080906148867, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=454, Percent_Identity=33.4801762114537, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324258, Length=461, Percent_Identity=27.114967462039, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=38.0530973451327, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI18859875, Length=461, Percent_Identity=27.765726681128, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI20129315, Length=450, Percent_Identity=28.6666666666667, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24582497, Length=441, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 61818; Mature: 61687

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEVNLPALGESVTEGTVTRWLKQVGDSVAVDEPLLEVSTDKVDTEIPSPIAGTLLEIR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCHHCCEEEEE
ANEDDTVEVGAVLAVIGDAGESSGDSGGAQAEAQPDPEPEPEAQPEPEPAQEQAQQEAQQ
CCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PAAEQPAPAAEAKPSGGSGTAVTLPALGESVTEGTVTRWLKSVGDEVAVDEPLLEVSTDK
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
VDTEIPSPVAGTLLEIKVAEDETVEVGAELAVIGSGQAAPAESKPEPTPEPEPEPTPEPE
CCCCCCCCCCCCEEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PEPEAAPEPEPAPAPQEQAAPPAPAPAAAAQPTAQPAAQPVASGDGPGYVTPLVRKMAAQ
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
HGVDLASVTGTGVGGRIRKQDVLDAAKAQQQAQQQAQQPAQAAPAAPAAPSAPVSSGAPS
HCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
PLRGTTVKMSRLRKIIATRMVDSLQTSAQLTQVVEVDVTNIARLRDEAKADFLSREGVKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
SYLPFFAKAAIDALKHHPALNATIDTEAGEVTYYDRENLAIAVDTEKGLITPVIKDAGDL
EHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHCCCCC
SIAGLAKKIADVAQRTRTNKIGPDELSGGTFTITNLGSVGALWDTPIVNKPQVAILGPGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCH
VVKRPVVIDDPNLGETIAVRYMVYLALTYDHQLVDGADAGRFLTDVKQRLESGQFEV
HHCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATEVNLPALGESVTEGTVTRWLKQVGDSVAVDEPLLEVSTDKVDTEIPSPIAGTLLEIR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCHHCCEEEEE
ANEDDTVEVGAVLAVIGDAGESSGDSGGAQAEAQPDPEPEPEAQPEPEPAQEQAQQEAQQ
CCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
PAAEQPAPAAEAKPSGGSGTAVTLPALGESVTEGTVTRWLKSVGDEVAVDEPLLEVSTDK
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCC
VDTEIPSPVAGTLLEIKVAEDETVEVGAELAVIGSGQAAPAESKPEPTPEPEPEPTPEPE
CCCCCCCCCCCCEEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PEPEAAPEPEPAPAPQEQAAPPAPAPAAAAQPTAQPAAQPVASGDGPGYVTPLVRKMAAQ
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
HGVDLASVTGTGVGGRIRKQDVLDAAKAQQQAQQQAQQPAQAAPAAPAAPSAPVSSGAPS
HCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCC
PLRGTTVKMSRLRKIIATRMVDSLQTSAQLTQVVEVDVTNIARLRDEAKADFLSREGVKL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
SYLPFFAKAAIDALKHHPALNATIDTEAGEVTYYDRENLAIAVDTEKGLITPVIKDAGDL
EHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCEEEEEECCCCCCCHHHHCCCCC
SIAGLAKKIADVAQRTRTNKIGPDELSGGTFTITNLGSVGALWDTPIVNKPQVAILGPGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCH
VVKRPVVIDDPNLGETIAVRYMVYLALTYDHQLVDGADAGRFLTDVKQRLESGQFEV
HHCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]