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The map label for this gene is priA [H]

Identifier: 119357344

GI number: 119357344

Start: 1750776

End: 1753208

Strand: Direct

Name: priA [H]

Synonym: Cpha266_1542

Alternate gene names: 119357344

Gene position: 1750776-1753208 (Clockwise)

Preceding gene: 119357343

Following gene: 119357345

Centisome position: 55.87

GC content: 46.12

Gene sequence:

>2433_bases
ATGCAGGCTGAAATCGTTATCGACAAGCTTTATCGGGGAGAACCGGTACGCATAGCTTTACCTGACTATCTTGTGAGGGA
ATTGAGCCCTGGCTGTATGGTGATGGTAACTTCAAGTAAAGGAAATAAAGCCACTTATCCTGCCTATATCCTGCGTCTTT
ATCAGGATAACGCCGATATCCCCGAAACTCTGGTCATTTCCGATGTACTCTATGACGGCGTTCCTGTTTTAAGCCCTTCT
CTTTTGAAACTGACCGCCTGGATGGCCGAATACTACCTCACGACACCTCTTGACACCATTACATCGGCCCTGCCTGCTGC
CGTAAGAACCACGGTGAACGATATTGTCGAACTTTCCGGCTTTCAACTGCATGGAGCGACACCAAAAATCGTCAATACTT
CTCTCCGCCGCTCCATACTCAAACTTATCGGGCAAGAAAAAAAGCTCACCGTACGTCAACTGGAAAAACGACTTGGCAAA
AAGGATCTCTACCGAGCGTTAAGCGAACTTGAGCAGGCAGGCCATCTCACCCTGCAAAAAAAATTCTCATCGACAAAGCC
GAGAGAAAAAACTGCTTATCGACTTTCTGTGCCGATACCGGAAAAAATCGAACTGATCCTTCATGTCGCCCCCAGACAAC
TCGAAGCGTTCAAGGCTCTTGCAACATTCAACAATACACCGACGTTTCCTGAAACCCTTGGAATTTCGAGAGATCACCTC
AATGCTCTGGTAAAAAAAGGCCTTGCCGAAAAAGTTCAGATTGAACTATCGAGCTCGTTCAGGTCAGGTTTCTCGGAACC
ACCCAAACCCGTTGAAACGCTCTCCAATGCCCAGCAAAACGCACTGCAAACCCTTTCGGATGCCTATAAAAAACAAGAGT
TCGCAACCTTTTTGCTCCATGGCGTTACCGGAAGCGGCAAAACACTTGTTTATATTGAGTTCCTTAAACAGGTAATCGCA
TCCGGAAAAACAGCAATAGTTCTTGTACCGGAAATTGCTCTTACCCCTCAGACAGCCGCCCGATTTCGTCACCATTTTCA
TGATGACATTACGATTCTGCACAGTGCAATGAGCGACCATGAAAAATACGATGCATGGCATAACCTCCGTCTGGGAAAAA
CAAAAATTGCCCTCGGTGCCCGTTCCTCCGTATTTGCTCCTCTTGATAATCTTGGAGCTATCATTGTTGATGAAGAGCAT
GACGGAGCCTATAAACAGGATCGTAACCCGCGGTATCAGGGGAGAGATACCGCCATCATGAGAGCAATGTTTGAAAATGC
GCTCTGTGTTCTGGGAACCGCAACCCCGTCTTTTGAATCATATCACAATGCTCTTAACGGAAAATACACACTTATCACCC
TTCCGGAGAGAATCGACGGTGCAAAAATGCCCGAAATCAAAATCATCTGGATGCGTGAAAATCCGAAAGCTTCGCGATCC
ATTTCGGAAACGCTCTATCAGGCAATAAAAACTCGACTTGAAAAAAACGAGCAGGTCATTCTGCTGCAAAATCGAAGAGG
ATTTGCCGGCAGTGTTTTTTGCCTTGATTGCGGTAACACCCCTTCGTGTAAATTCTGCAATATTCCTCTGGTCTATCATT
CAAAAGAGCAACATCTTCGCTGTCATTACTGCGGCTTTGTCACTCCATTTACAGATGCGTGCAACGCTTGCGCATCAAAA
AATCTCCTTTACAAAAGCAGCGGCACTGAAAGAATCGAGGAAGAGCTGAATTTGCTTTTCCCTGACGAATTGATCTTGCG
GATGGATGTTGACACAACTTCGAGCAAAGACGCTCACGCAAAAATCCTTAAAGATTTTCAGGATAAAAAATCAAAAATCC
TGCTTGGCACTCAAATGGTTGCCAAAGGGCTTGATTTTCCGGATGTTACCCTTGTCGGCGTGCTTATGGCCGACATCGGG
CTCAACATACCTGATTTCCGAGCTTCTGAACGCCTTTTTTCTCTCTTGACCCAGGTGTCCGGACGGGCAGGACGTTCCTC
AAAATCAGGAGAAGTGCTTCTGCAGGTATTCAATAAAGATGCCGATATCTTTGCATCTCTCCTGAAGGGCAGCTATACGT
CATTTTTCGAGCAGGAAATGTCAACTCGAAAAGCGCTCCATTATCCACCGTTCTCAAAACTGGTCAAATTCGAATTCTCA
TCACCTGACGAAAAAAAGGCTGAAGAAGCATCGGCCTCATGGGCAGACATACTGCGACCAGCTTTAACATCTGAAACAGG
AATACTCCTTGGTCCGGCCCCGGCAGGAATGGCAAAGCTGAAAGGTCTCTATCGATACCATGTACTGGTAAAACTCTTCA
GCGGAAAACTTTCACCGGCCTTTCTTCATCAAGAACTCAATGAACTCAGTATGAGTTACCGCAAAGAAAAGCTCACGATC
AGCATTGATGTCGATCCTCAGAATCTGCTCTGA

Upstream 100 bases:

>100_bases
GCCAGAGAATACTTGATGCACTCAATTCCTGAAACCGGAAAATCGGCAGAATATCAAAAAAAAGTCAGTGTTTGCCACTG
GCTTTTTTTCTTTTAAACCT

Downstream 100 bases:

>100_bases
AAAACAAGTGAACGTTTGCATCTGAAGAGAACGAAAATATTCTCTATATTCGAAAATATTTTTATTCCTTAACACTTTGC
GGAAATAAACATTATGGACG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MQAEIVIDKLYRGEPVRIALPDYLVRELSPGCMVMVTSSKGNKATYPAYILRLYQDNADIPETLVISDVLYDGVPVLSPS
LLKLTAWMAEYYLTTPLDTITSALPAAVRTTVNDIVELSGFQLHGATPKIVNTSLRRSILKLIGQEKKLTVRQLEKRLGK
KDLYRALSELEQAGHLTLQKKFSSTKPREKTAYRLSVPIPEKIELILHVAPRQLEAFKALATFNNTPTFPETLGISRDHL
NALVKKGLAEKVQIELSSSFRSGFSEPPKPVETLSNAQQNALQTLSDAYKKQEFATFLLHGVTGSGKTLVYIEFLKQVIA
SGKTAIVLVPEIALTPQTAARFRHHFHDDITILHSAMSDHEKYDAWHNLRLGKTKIALGARSSVFAPLDNLGAIIVDEEH
DGAYKQDRNPRYQGRDTAIMRAMFENALCVLGTATPSFESYHNALNGKYTLITLPERIDGAKMPEIKIIWMRENPKASRS
ISETLYQAIKTRLEKNEQVILLQNRRGFAGSVFCLDCGNTPSCKFCNIPLVYHSKEQHLRCHYCGFVTPFTDACNACASK
NLLYKSSGTERIEEELNLLFPDELILRMDVDTTSSKDAHAKILKDFQDKKSKILLGTQMVAKGLDFPDVTLVGVLMADIG
LNIPDFRASERLFSLLTQVSGRAGRSSKSGEVLLQVFNKDADIFASLLKGSYTSFFEQEMSTRKALHYPPFSKLVKFEFS
SPDEKKAEEASASWADILRPALTSETGILLGPAPAGMAKLKGLYRYHVLVKLFSGKLSPAFLHQELNELSMSYRKEKLTI
SIDVDPQNLL

Sequences:

>Translated_810_residues
MQAEIVIDKLYRGEPVRIALPDYLVRELSPGCMVMVTSSKGNKATYPAYILRLYQDNADIPETLVISDVLYDGVPVLSPS
LLKLTAWMAEYYLTTPLDTITSALPAAVRTTVNDIVELSGFQLHGATPKIVNTSLRRSILKLIGQEKKLTVRQLEKRLGK
KDLYRALSELEQAGHLTLQKKFSSTKPREKTAYRLSVPIPEKIELILHVAPRQLEAFKALATFNNTPTFPETLGISRDHL
NALVKKGLAEKVQIELSSSFRSGFSEPPKPVETLSNAQQNALQTLSDAYKKQEFATFLLHGVTGSGKTLVYIEFLKQVIA
SGKTAIVLVPEIALTPQTAARFRHHFHDDITILHSAMSDHEKYDAWHNLRLGKTKIALGARSSVFAPLDNLGAIIVDEEH
DGAYKQDRNPRYQGRDTAIMRAMFENALCVLGTATPSFESYHNALNGKYTLITLPERIDGAKMPEIKIIWMRENPKASRS
ISETLYQAIKTRLEKNEQVILLQNRRGFAGSVFCLDCGNTPSCKFCNIPLVYHSKEQHLRCHYCGFVTPFTDACNACASK
NLLYKSSGTERIEEELNLLFPDELILRMDVDTTSSKDAHAKILKDFQDKKSKILLGTQMVAKGLDFPDVTLVGVLMADIG
LNIPDFRASERLFSLLTQVSGRAGRSSKSGEVLLQVFNKDADIFASLLKGSYTSFFEQEMSTRKALHYPPFSKLVKFEFS
SPDEKKAEEASASWADILRPALTSETGILLGPAPAGMAKLKGLYRYHVLVKLFSGKLSPAFLHQELNELSMSYRKEKLTI
SIDVDPQNLL
>Mature_810_residues
MQAEIVIDKLYRGEPVRIALPDYLVRELSPGCMVMVTSSKGNKATYPAYILRLYQDNADIPETLVISDVLYDGVPVLSPS
LLKLTAWMAEYYLTTPLDTITSALPAAVRTTVNDIVELSGFQLHGATPKIVNTSLRRSILKLIGQEKKLTVRQLEKRLGK
KDLYRALSELEQAGHLTLQKKFSSTKPREKTAYRLSVPIPEKIELILHVAPRQLEAFKALATFNNTPTFPETLGISRDHL
NALVKKGLAEKVQIELSSSFRSGFSEPPKPVETLSNAQQNALQTLSDAYKKQEFATFLLHGVTGSGKTLVYIEFLKQVIA
SGKTAIVLVPEIALTPQTAARFRHHFHDDITILHSAMSDHEKYDAWHNLRLGKTKIALGARSSVFAPLDNLGAIIVDEEH
DGAYKQDRNPRYQGRDTAIMRAMFENALCVLGTATPSFESYHNALNGKYTLITLPERIDGAKMPEIKIIWMRENPKASRS
ISETLYQAIKTRLEKNEQVILLQNRRGFAGSVFCLDCGNTPSCKFCNIPLVYHSKEQHLRCHYCGFVTPFTDACNACASK
NLLYKSSGTERIEEELNLLFPDELILRMDVDTTSSKDAHAKILKDFQDKKSKILLGTQMVAKGLDFPDVTLVGVLMADIG
LNIPDFRASERLFSLLTQVSGRAGRSSKSGEVLLQVFNKDADIFASLLKGSYTSFFEQEMSTRKALHYPPFSKLVKFEFS
SPDEKKAEEASASWADILRPALTSETGILLGPAPAGMAKLKGLYRYHVLVKLFSGKLSPAFLHQELNELSMSYRKEKLTI
SIDVDPQNLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=599, Percent_Identity=37.0617696160267, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90395; Mature: 90395

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAEIVIDKLYRGEPVRIALPDYLVRELSPGCMVMVTSSKGNKATYPAYILRLYQDNADI
CCCCEEEHHHHCCCCEEEECHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCC
PETLVISDVLYDGVPVLSPSLLKLTAWMAEYYLTTPLDTITSALPAAVRTTVNDIVELSG
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
FQLHGATPKIVNTSLRRSILKLIGQEKKLTVRQLEKRLGKKDLYRALSELEQAGHLTLQK
EEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEH
KFSSTKPREKTAYRLSVPIPEKIELILHVAPRQLEAFKALATFNNTPTFPETLGISRDHL
HHCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHH
NALVKKGLAEKVQIELSSSFRSGFSEPPKPVETLSNAQQNALQTLSDAYKKQEFATFLLH
HHHHHCCCHHHEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVTGSGKTLVYIEFLKQVIASGKTAIVLVPEIALTPQTAARFRHHFHDDITILHSAMSDH
CCCCCCCEEEHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
EKYDAWHNLRLGKTKIALGARSSVFAPLDNLGAIIVDEEHDGAYKQDRNPRYQGRDTAIM
HHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
RAMFENALCVLGTATPSFESYHNALNGKYTLITLPERIDGAKMPEIKIIWMRENPKASRS
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHH
ISETLYQAIKTRLEKNEQVILLQNRRGFAGSVFCLDCGNTPSCKFCNIPLVYHSKEQHLR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEECCEEEECCCCCCCE
CHYCGFVTPFTDACNACASKNLLYKSSGTERIEEELNLLFPDELILRMDVDTTSSKDAHA
EEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHH
KILKDFQDKKSKILLGTQMVAKGLDFPDVTLVGVLMADIGLNIPDFRASERLFSLLTQVS
HHHHHHHHCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GRAGRSSKSGEVLLQVFNKDADIFASLLKGSYTSFFEQEMSTRKALHYPPFSKLVKFEFS
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHEEEECC
SPDEKKAEEASASWADILRPALTSETGILLGPAPAGMAKLKGLYRYHVLVKLFSGKLSPA
CCCHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
FLHQELNELSMSYRKEKLTISIDVDPQNLL
HHHHHHHHHHHHHCCCEEEEEEECCHHHCC
>Mature Secondary Structure
MQAEIVIDKLYRGEPVRIALPDYLVRELSPGCMVMVTSSKGNKATYPAYILRLYQDNADI
CCCCEEEHHHHCCCCEEEECHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCC
PETLVISDVLYDGVPVLSPSLLKLTAWMAEYYLTTPLDTITSALPAAVRTTVNDIVELSG
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
FQLHGATPKIVNTSLRRSILKLIGQEKKLTVRQLEKRLGKKDLYRALSELEQAGHLTLQK
EEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCEEEEH
KFSSTKPREKTAYRLSVPIPEKIELILHVAPRQLEAFKALATFNNTPTFPETLGISRDHL
HHCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHH
NALVKKGLAEKVQIELSSSFRSGFSEPPKPVETLSNAQQNALQTLSDAYKKQEFATFLLH
HHHHHCCCHHHEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVTGSGKTLVYIEFLKQVIASGKTAIVLVPEIALTPQTAARFRHHFHDDITILHSAMSDH
CCCCCCCEEEHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
EKYDAWHNLRLGKTKIALGARSSVFAPLDNLGAIIVDEEHDGAYKQDRNPRYQGRDTAIM
HHHHHHHCCCCCCEEEEECCCCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
RAMFENALCVLGTATPSFESYHNALNGKYTLITLPERIDGAKMPEIKIIWMRENPKASRS
HHHHHCCEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHHHH
ISETLYQAIKTRLEKNEQVILLQNRRGFAGSVFCLDCGNTPSCKFCNIPLVYHSKEQHLR
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEECCEEEECCCCCCCE
CHYCGFVTPFTDACNACASKNLLYKSSGTERIEEELNLLFPDELILRMDVDTTSSKDAHA
EEEECCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHH
KILKDFQDKKSKILLGTQMVAKGLDFPDVTLVGVLMADIGLNIPDFRASERLFSLLTQVS
HHHHHHHHCCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
GRAGRSSKSGEVLLQVFNKDADIFASLLKGSYTSFFEQEMSTRKALHYPPFSKLVKFEFS
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHEEEECC
SPDEKKAEEASASWADILRPALTSETGILLGPAPAGMAKLKGLYRYHVLVKLFSGKLSPA
CCCHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
FLHQELNELSMSYRKEKLTISIDVDPQNLL
HHHHHHHHHHHHHCCCEEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]