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The map label for this gene is mltD [H]

Identifier: 119356908

GI number: 119356908

Start: 1229626

End: 1231578

Strand: Direct

Name: mltD [H]

Synonym: Cpha266_1087

Alternate gene names: 119356908

Gene position: 1229626-1231578 (Clockwise)

Preceding gene: 119356907

Following gene: 119356909

Centisome position: 39.24

GC content: 46.9

Gene sequence:

>1953_bases
ATGTTAAACCGACCGATAGTGTTCTGCGGAACAGCAACAGAGAGTTTCAGTAATCCCCCAGATAACGTTAGGTTGATAGT
GAAGAGACGGATTTATTACACATTGATGACGGCCTGGTTGTCGCTATCGGCTATTCTTTTTTCCGCAGAGTCTCTCCTTG
CAAGGAATTCTCGTGACGATAGAGATGGCAATTCCGGAAAAACATCCGTAACGGAGATTCTCGACAGCCTTGTTTACACC
ACGTATTTCAAGGATGAGCATTTTTCTCCTTCAAAAAAAGAGCCCTATCGTCCCGGTTTACCCAGCCATTTTGTTCCCCA
GTTCAGCGATTCGGTCTATGCGGCAAGGATATCAGCCCTGGATCGTAAAACTGCGTTTAATCTGGTTTATAACGAGCATG
TGAGGGGATTTATCAGGATTTATGCTGTTGACAAGCGAAACATGACTGCCAAGATTCTTGGGCTCAGCAAGATTTATTTT
CCGCTTTTCGAAGAGAATCTTGAACGGTACAATGTTCCCCTTGAGATGAAATACCTTGCTATCGTTGAGTCGGCATTAAA
TCCCACAGCCGTTTCTTCCGCAGGTGCCAGAGGGTTGTGGCAGTTCATGTATGGGACAGGCAAGATGTATGGTCTTGAGA
CCTCTTCATTTATTGAAGATCGCTATGATCCCTACAAGGCTACTGCTGCGGCAAGCAAGCACCTTCGGGATCTTTACAAT
ATTTATGGAGACTGGTTTCTCGCCCTTGCAGCCTATAACTCAGGGCCGGGAAACGTAAACAAGGCGATCCGTCGGGCAGG
AGGTATCAAGAACTACTGGGCAATATGGAACTATCTTCCTGCTGAAACGCGCGGGTATGTGCCGGCTTTTATTGCCGTCA
ATTACATTATGAATTACTATAAAGAGCACAATATTCAGCCGATAGAGCCGGGTTTTCTCTACAATGAAATTGACGCACTG
AAAATCACAAGGCTTGTTTCGTTTGAACAGATCAACGAGACGATCGGCGTGCCGATGAAAGATCTCCAGTTTCTCAATCC
CCAGTATAAGCTTGGTCTTATACCGGGCACTTCCTATAGCGGCAATATGCTGAGGCTTCCGAGAAAATATGTCGGTCAGT
TTCAGCGTCGCGAAGAAGAGATTTATGCTTATAAATCCGAGCAGGTTATTGAGCGGGCTCAGCTTCTTGCAAGAGTCCAG
AGTATGGATGGAGGAGGATCGGTAAGTTCAGCCCAGAGTGCGGGCGGAACGATGAGAATGAAAATGCATGAGGTTCGACA
GGGCGAAACACTTGGCTCGATAGCTCGTGTGTATCGATGTGATGTCAGTCAGCTTATCCGCTGGAATGACCTTAAAGTCT
CCGAGGTTTCTCCAGGGCAGCGCCTCATTGTTTTTGCGGTGCCTGAAAGCTCCACTGCCCAGGGGGTATCGGTACAGCAG
ACCGCCTCTGCCCGGGGATTATCTGCGCCATTGCTTCCTGCGCCGATTTTTCCTCCCGAACAGGTTCAGCGGCAAGCGCC
AATTGTTATGAAGCCGAGAGCTCAGGAACCAAAAGCCCAGGAGCAGAAGGTTGCTTTGCGAAAAGAAAAAATTCAAAACC
ATAAGGTTAAAAAAGGGGAGACCGTTGCCTCAATTTCCCGCCAATATGGAGTGAGTTCCGAAAGTATTGCCGAGCTGAAT
AATTTGAGCGGCAGAAAACAGATTCGGCCTGGACAGGTGCTGAAAATAACCGGCAAGGCATCGTCAGCAAAATCAGAAGC
TCGCATATCTTCAGCAAAAAAAGAGTCAGGATCTTCTCGAAGCCATAAATATCACAAGGTTAAAAAGGGGGAAACTCTTT
CATCGATAGCCGATAAGTATGCTTTGAGTGTTTCGGAGCTGAGCAAAAAAAACAATCTCGGAAAGAAAAAACTTATACGC
CCAGGACAGAAACTCAATGTTACCCGCGAGTAG

Upstream 100 bases:

>100_bases
AACGGTCTTTTTTTGTGGAAGATCACCTATGGAAGTGATTCTCATTCCTTCTGAGAAAGGATAGATCGGTTATTGTTTGT
ATTAGTCAGGAACTGTTTTT

Downstream 100 bases:

>100_bases
TCAGCGGTTGTTTTTTATAAATCTATACTCTTTGAGCCGGTTTTTTCGAAAACGGAGCGGATTGCTTTATTCCTGTGGGT
AATAAACAGGATTTTAATTA

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MLNRPIVFCGTATESFSNPPDNVRLIVKRRIYYTLMTAWLSLSAILFSAESLLARNSRDDRDGNSGKTSVTEILDSLVYT
TYFKDEHFSPSKKEPYRPGLPSHFVPQFSDSVYAARISALDRKTAFNLVYNEHVRGFIRIYAVDKRNMTAKILGLSKIYF
PLFEENLERYNVPLEMKYLAIVESALNPTAVSSAGARGLWQFMYGTGKMYGLETSSFIEDRYDPYKATAAASKHLRDLYN
IYGDWFLALAAYNSGPGNVNKAIRRAGGIKNYWAIWNYLPAETRGYVPAFIAVNYIMNYYKEHNIQPIEPGFLYNEIDAL
KITRLVSFEQINETIGVPMKDLQFLNPQYKLGLIPGTSYSGNMLRLPRKYVGQFQRREEEIYAYKSEQVIERAQLLARVQ
SMDGGGSVSSAQSAGGTMRMKMHEVRQGETLGSIARVYRCDVSQLIRWNDLKVSEVSPGQRLIVFAVPESSTAQGVSVQQ
TASARGLSAPLLPAPIFPPEQVQRQAPIVMKPRAQEPKAQEQKVALRKEKIQNHKVKKGETVASISRQYGVSSESIAELN
NLSGRKQIRPGQVLKITGKASSAKSEARISSAKKESGSSRSHKYHKVKKGETLSSIADKYALSVSELSKKNNLGKKKLIR
PGQKLNVTRE

Sequences:

>Translated_650_residues
MLNRPIVFCGTATESFSNPPDNVRLIVKRRIYYTLMTAWLSLSAILFSAESLLARNSRDDRDGNSGKTSVTEILDSLVYT
TYFKDEHFSPSKKEPYRPGLPSHFVPQFSDSVYAARISALDRKTAFNLVYNEHVRGFIRIYAVDKRNMTAKILGLSKIYF
PLFEENLERYNVPLEMKYLAIVESALNPTAVSSAGARGLWQFMYGTGKMYGLETSSFIEDRYDPYKATAAASKHLRDLYN
IYGDWFLALAAYNSGPGNVNKAIRRAGGIKNYWAIWNYLPAETRGYVPAFIAVNYIMNYYKEHNIQPIEPGFLYNEIDAL
KITRLVSFEQINETIGVPMKDLQFLNPQYKLGLIPGTSYSGNMLRLPRKYVGQFQRREEEIYAYKSEQVIERAQLLARVQ
SMDGGGSVSSAQSAGGTMRMKMHEVRQGETLGSIARVYRCDVSQLIRWNDLKVSEVSPGQRLIVFAVPESSTAQGVSVQQ
TASARGLSAPLLPAPIFPPEQVQRQAPIVMKPRAQEPKAQEQKVALRKEKIQNHKVKKGETVASISRQYGVSSESIAELN
NLSGRKQIRPGQVLKITGKASSAKSEARISSAKKESGSSRSHKYHKVKKGETLSSIADKYALSVSELSKKNNLGKKKLIR
PGQKLNVTRE
>Mature_650_residues
MLNRPIVFCGTATESFSNPPDNVRLIVKRRIYYTLMTAWLSLSAILFSAESLLARNSRDDRDGNSGKTSVTEILDSLVYT
TYFKDEHFSPSKKEPYRPGLPSHFVPQFSDSVYAARISALDRKTAFNLVYNEHVRGFIRIYAVDKRNMTAKILGLSKIYF
PLFEENLERYNVPLEMKYLAIVESALNPTAVSSAGARGLWQFMYGTGKMYGLETSSFIEDRYDPYKATAAASKHLRDLYN
IYGDWFLALAAYNSGPGNVNKAIRRAGGIKNYWAIWNYLPAETRGYVPAFIAVNYIMNYYKEHNIQPIEPGFLYNEIDAL
KITRLVSFEQINETIGVPMKDLQFLNPQYKLGLIPGTSYSGNMLRLPRKYVGQFQRREEEIYAYKSEQVIERAQLLARVQ
SMDGGGSVSSAQSAGGTMRMKMHEVRQGETLGSIARVYRCDVSQLIRWNDLKVSEVSPGQRLIVFAVPESSTAQGVSVQQ
TASARGLSAPLLPAPIFPPEQVQRQAPIVMKPRAQEPKAQEQKVALRKEKIQNHKVKKGETVASISRQYGVSSESIAELN
NLSGRKQIRPGQVLKITGKASSAKSEARISSAKKESGSSRSHKYHKVKKGETLSSIADKYALSVSELSKKNNLGKKKLIR
PGQKLNVTRE

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=428, Percent_Identity=27.1028037383178, Blast_Score=128, Evalue=1e-30,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 72962; Mature: 72962

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRPIVFCGTATESFSNPPDNVRLIVKRRIYYTLMTAWLSLSAILFSAESLLARNSRDD
CCCCCEEEEECCHHCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RDGNSGKTSVTEILDSLVYTTYFKDEHFSPSKKEPYRPGLPSHFVPQFSDSVYAARISAL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DRKTAFNLVYNEHVRGFIRIYAVDKRNMTAKILGLSKIYFPLFEENLERYNVPLEMKYLA
HHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEHHHHH
IVESALNPTAVSSAGARGLWQFMYGTGKMYGLETSSFIEDRYDPYKATAAASKHLRDLYN
HHHHHCCCCHHCCCCHHHHHHHHHCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHH
IYGDWFLALAAYNSGPGNVNKAIRRAGGIKNYWAIWNYLPAETRGYVPAFIAVNYIMNYY
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KEHNIQPIEPGFLYNEIDALKITRLVSFEQINETIGVPMKDLQFLNPQYKLGLIPGTSYS
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCC
GNMLRLPRKYVGQFQRREEEIYAYKSEQVIERAQLLARVQSMDGGGSVSSAQSAGGTMRM
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEE
KMHEVRQGETLGSIARVYRCDVSQLIRWNDLKVSEVSPGQRLIVFAVPESSTAQGVSVQQ
HHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCHHH
TASARGLSAPLLPAPIFPPEQVQRQAPIVMKPRAQEPKAQEQKVALRKEKIQNHKVKKGE
HHCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TVASISRQYGVSSESIAELNNLSGRKQIRPGQVLKITGKASSAKSEARISSAKKESGSSR
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
SHKYHKVKKGETLSSIADKYALSVSELSKKNNLGKKKLIRPGQKLNVTRE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLNRPIVFCGTATESFSNPPDNVRLIVKRRIYYTLMTAWLSLSAILFSAESLLARNSRDD
CCCCCEEEEECCHHCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RDGNSGKTSVTEILDSLVYTTYFKDEHFSPSKKEPYRPGLPSHFVPQFSDSVYAARISAL
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
DRKTAFNLVYNEHVRGFIRIYAVDKRNMTAKILGLSKIYFPLFEENLERYNVPLEMKYLA
HHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEHHHHH
IVESALNPTAVSSAGARGLWQFMYGTGKMYGLETSSFIEDRYDPYKATAAASKHLRDLYN
HHHHHCCCCHHCCCCHHHHHHHHHCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHH
IYGDWFLALAAYNSGPGNVNKAIRRAGGIKNYWAIWNYLPAETRGYVPAFIAVNYIMNYY
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KEHNIQPIEPGFLYNEIDALKITRLVSFEQINETIGVPMKDLQFLNPQYKLGLIPGTSYS
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCC
GNMLRLPRKYVGQFQRREEEIYAYKSEQVIERAQLLARVQSMDGGGSVSSAQSAGGTMRM
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEE
KMHEVRQGETLGSIARVYRCDVSQLIRWNDLKVSEVSPGQRLIVFAVPESSTAQGVSVQQ
HHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCCCCCHHH
TASARGLSAPLLPAPIFPPEQVQRQAPIVMKPRAQEPKAQEQKVALRKEKIQNHKVKKGE
HHCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
TVASISRQYGVSSESIAELNNLSGRKQIRPGQVLKITGKASSAKSEARISSAKKESGSSR
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
SHKYHKVKKGETLSSIADKYALSVSELSKKNNLGKKKLIRPGQKLNVTRE
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]