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The map label for this gene is truA

Identifier: 119356907

GI number: 119356907

Start: 1228803

End: 1229579

Strand: Direct

Name: truA

Synonym: Cpha266_1086

Alternate gene names: 119356907

Gene position: 1228803-1229579 (Clockwise)

Preceding gene: 119356900

Following gene: 119356908

Centisome position: 39.21

GC content: 48.52

Gene sequence:

>777_bases
ATGTTAGCTAAAGATTTTCTTTTTATGAAGAACATCAGGATTACCATTGAATATGATGGAACCGGATATGCCGGGTGGCA
GAGACAGGCAGGCTCTTTTTGCACGGTGCAGGGAGAGATTGAGCGTATTCTCAGTCAGATATTACAGGAAGATATACGGC
TTGCCGGGGCAGGACGAACTGATAAGGGCGTTCATGCCCGGGCACAGATCGCCACGTTCAAAAGTCGTTCATCCCTGGGG
CCAGACAGAATGGTTCACTCAATGAATGCGCTGTTACCAAACACCATAAGAATCAGTGATCCCCTCGTGGTTCCGGAGGG
GTTTCATGCCCGTCATAGCGCAAAAGAAAGAGAGTACAGGTATTTTGTTCGTGAAGAGCCATCTGCGGTGCTTGGGCGTT
TCAGCGGATGTTCTTATGGTTCGCTGGATCTCATGTCCATGAACCGGGTAGCATCTTATGTTCTTGGTGAGCATGACTTT
TCCGTTTTTTCAAAAGAAACACGAGACAGGACGGGGTGTCTGTGCAGGGTTATCTCCTGCAAATGGTTTCGTCAACGGGA
ATTTTTTGTGCTCAGGATCTCGGCTAACCGATTTCTGCGGTCAATGGTGCGTTATCTGGCGGCCTTGATGATTGATTCAG
GCAAAGGTCTTCTTTCTCCCGAAGAAGCCAGGGAGATGATTGAGTCCGGCATCCTGACAAGGCAACTTGTGCCGGCAGCA
CCAAACGGTCTTTTTTTGTGGAAGATCACCTATGGAAGTGATTCTCATTCCTTCTGA

Upstream 100 bases:

>100_bases
AGAAGTAAATAGCTTTGTGCCTTGAAAAAGTTCTGTGTCAGTAGTTTATAGGTACCCGGCTGAAGCTTTTTATAGGCACC
TTTGTTTATTCATAAACAGC

Downstream 100 bases:

>100_bases
GAAAGGATAGATCGGTTATTGTTTGTATTAGTCAGGAACTGTTTTTATGTTAAACCGACCGATAGTGTTCTGCGGAACAG
CAACAGAGAGTTTCAGTAAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKDFLFMKNIRITIEYDGTGYAGWQRQAGSFCTVQGEIERILSQILQEDIRLAGAGRTDKGVHARAQIATFKSRSSLG
PDRMVHSMNALLPNTIRISDPLVVPEGFHARHSAKEREYRYFVREEPSAVLGRFSGCSYGSLDLMSMNRVASYVLGEHDF
SVFSKETRDRTGCLCRVISCKWFRQREFFVLRISANRFLRSMVRYLAALMIDSGKGLLSPEEAREMIESGILTRQLVPAA
PNGLFLWKITYGSDSHSF

Sequences:

>Translated_258_residues
MLAKDFLFMKNIRITIEYDGTGYAGWQRQAGSFCTVQGEIERILSQILQEDIRLAGAGRTDKGVHARAQIATFKSRSSLG
PDRMVHSMNALLPNTIRISDPLVVPEGFHARHSAKEREYRYFVREEPSAVLGRFSGCSYGSLDLMSMNRVASYVLGEHDF
SVFSKETRDRTGCLCRVISCKWFRQREFFVLRISANRFLRSMVRYLAALMIDSGKGLLSPEEAREMIESGILTRQLVPAA
PNGLFLWKITYGSDSHSF
>Mature_258_residues
MLAKDFLFMKNIRITIEYDGTGYAGWQRQAGSFCTVQGEIERILSQILQEDIRLAGAGRTDKGVHARAQIATFKSRSSLG
PDRMVHSMNALLPNTIRISDPLVVPEGFHARHSAKEREYRYFVREEPSAVLGRFSGCSYGSLDLMSMNRVASYVLGEHDF
SVFSKETRDRTGCLCRVISCKWFRQREFFVLRISANRFLRSMVRYLAALMIDSGKGLLSPEEAREMIESGILTRQLVPAA
PNGLFLWKITYGSDSHSF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=27.1739130434783, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788657, Length=244, Percent_Identity=34.4262295081967, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321112, Length=246, Percent_Identity=30.0813008130081, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLPD (A1BFF0)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911551.1
- ProteinModelPortal:   A1BFF0
- SMR:   A1BFF0
- STRING:   A1BFF0
- GeneID:   4570030
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_1086
- NMPDR:   fig|290317.7.peg.1152
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29236; Mature: 29236

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKDFLFMKNIRITIEYDGTGYAGWQRQAGSFCTVQGEIERILSQILQEDIRLAGAGRT
CCCCHHEEEECEEEEEEECCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
DKGVHARAQIATFKSRSSLGPDRMVHSMNALLPNTIRISDPLVVPEGFHARHSAKEREYR
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCEECCCCCCCHHHHHHHHHH
YFVREEPSAVLGRFSGCSYGSLDLMSMNRVASYVLGEHDFSVFSKETRDRTGCLCRVISC
HHHHCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
KWFRQREFFVLRISANRFLRSMVRYLAALMIDSGKGLLSPEEAREMIESGILTRQLVPAA
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCC
PNGLFLWKITYGSDSHSF
CCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MLAKDFLFMKNIRITIEYDGTGYAGWQRQAGSFCTVQGEIERILSQILQEDIRLAGAGRT
CCCCHHEEEECEEEEEEECCCCCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
DKGVHARAQIATFKSRSSLGPDRMVHSMNALLPNTIRISDPLVVPEGFHARHSAKEREYR
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCEECCCCCCCHHHHHHHHHH
YFVREEPSAVLGRFSGCSYGSLDLMSMNRVASYVLGEHDFSVFSKETRDRTGCLCRVISC
HHHHCCCHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHH
KWFRQREFFVLRISANRFLRSMVRYLAALMIDSGKGLLSPEEAREMIESGILTRQLVPAA
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCCC
PNGLFLWKITYGSDSHSF
CCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA