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The map label for this gene is nagE [H]

Identifier: 118476189

GI number: 118476189

Start: 495981

End: 497492

Strand: Direct

Name: nagE [H]

Synonym: BALH_0438

Alternate gene names: 118476189

Gene position: 495981-497492 (Clockwise)

Preceding gene: 118476186

Following gene: 118476190

Centisome position: 9.43

GC content: 38.29

Gene sequence:

>1512_bases
TTGATAGGGGGAATTAAAATGTTGCAGTTTCTACAACGTATTGGTAAAGCGTTAATGCTTCCAATCGCCGTACTACCAGC
AGCAGGATTATTGCTTCGTTTAGGACAAGAAGACGTATTTAACATTCCTGTTATGGCACAGGCCGGTGCAGCAATTTTTG
ATAATTTAGCACTTATTTTTGCAATTGGTGTTGCAATCGGTTTGTCTGTTGACGGTAGTGGGGCAGCTGGGCTTGCCGGA
GCAATCGGATATCTTGTTTTACAAAATACAACGAATGCTCTAAGTAAGACGTATTCAGCAGCAGAGTTAAATGATAAATT
AAAAAGTGTTCAAGATTTAGTCGGTTCAGTAGATCCAACTAAATTAGCAGATACAATGACAAAGGTTTCAAAAGCAGCGG
CGTTAACGCCAAAAATAAATATGGCCATACTCGGTGGTATTATTGCAGGGGTTGTTGCGGGATTACTATACAACAAATTC
CATAAGATTAAACTACCAGAATGGTTAGGATTCTTTGCAGGAAAACGCTTCGTACCAATCATTACTTCAATCGTAATGTT
ACTTTTAGGATTAGTATTCGGTCAAATTTGGCCAACAATTCAAAGTGGTATTGATGCAGTGGCACATGGTATCGTGAACT
TAGGTTCAATTGGTGCTGGTTTATTTGGATTATTAAACCGTTTATTAATTCCAATTGGTTTACACCACGTAATGAACACA
TACTTCTGGTTCGTACTTGGTGACTTTACAAATGCAGCTGGCGATATTGTTCATGGTGATATTGCACGTTTCTTTGCAAA
AGATCCATCAGCAGGTATGTTTATGACTGGTTTCTTCCCAGTTATGATGTTCGGTTTACCAGCAGCATGTTTCGCAATGA
TTGCAGCTGCTAAACCAGAAAAACGTAAAATGGTTACAGGTATGTTAGGTGGTCTAGCATTAACTTCATTCTTAACTGGT
ATTACAGAGCCAATTGAATTCTCATTCATGTTCTTATCACCAGTATTATATGGAATTCATGCTGTATTAACAGGTCTGTC
TCTATTCATTACAACAACACTTGGCATTCATGATGGTTTCTCATTTAGTGCCGGGGCAATCGATTACGTTTTAAACTTCG
GTATTGCAACAAAACCATTGTTACTAGCAGGAATCGGTTTAATTTACGCAGCAATTTACTTTGTAGTATTCTACTTCTTA
ATTAAGAAGTTCGACCTAAAAACTCCTGGTCGTGAAGATGAAGAGGAAGTAGCTGAAGGCGAAGAAGCTCCAGTTGCAGG
TTCAATTGGTGAAACTTACGTAGCAGCTTTAGGTGGAAAAGAAAACTTAACAGTTATTGATAACTGTGCAACACGTCTAC
GCTTACAAGTGAAAGATGCTGGTCAAGTAAACGAAGCAGCATTAAAACGTGCTGGTGCAAAAGGTGTTATGAAATTAAGT
AACACGAGTGTCCAAGTTATCGTAGGTACAAATGTTGAATCTGTTGCCGATGATATGAAAAAACACGTATAA

Upstream 100 bases:

>100_bases
AAAATAAAAACTACACATCTATACAACTATATGTTATTATGGTTATGTAAATAGAAAGCGTTTTCTAAGGCGTACTTATA
ATGATAACGATTTCATAAAT

Downstream 100 bases:

>100_bases
ATAATTAGATAAGAGGATATCCAAAAAGATTGATAACATCAATTTTTTAGAGGGTATCCTCTTTTTTATGTTAAAAATTT
AACGGGAATCTTACTTTTGT

Product: PTS system N-acetylglucosamine-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MIGGIKMLQFLQRIGKALMLPIAVLPAAGLLLRLGQEDVFNIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAG
AIGYLVLQNTTNALSKTYSAAELNDKLKSVQDLVGSVDPTKLADTMTKVSKAAALTPKINMAILGGIIAGVVAGLLYNKF
HKIKLPEWLGFFAGKRFVPIITSIVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGAGLFGLLNRLLIPIGLHHVMNT
YFWFVLGDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLGGLALTSFLTG
ITEPIEFSFMFLSPVLYGIHAVLTGLSLFITTTLGIHDGFSFSAGAIDYVLNFGIATKPLLLAGIGLIYAAIYFVVFYFL
IKKFDLKTPGREDEEEVAEGEEAPVAGSIGETYVAALGGKENLTVIDNCATRLRLQVKDAGQVNEAALKRAGAKGVMKLS
NTSVQVIVGTNVESVADDMKKHV

Sequences:

>Translated_503_residues
MIGGIKMLQFLQRIGKALMLPIAVLPAAGLLLRLGQEDVFNIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAG
AIGYLVLQNTTNALSKTYSAAELNDKLKSVQDLVGSVDPTKLADTMTKVSKAAALTPKINMAILGGIIAGVVAGLLYNKF
HKIKLPEWLGFFAGKRFVPIITSIVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGAGLFGLLNRLLIPIGLHHVMNT
YFWFVLGDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLGGLALTSFLTG
ITEPIEFSFMFLSPVLYGIHAVLTGLSLFITTTLGIHDGFSFSAGAIDYVLNFGIATKPLLLAGIGLIYAAIYFVVFYFL
IKKFDLKTPGREDEEEVAEGEEAPVAGSIGETYVAALGGKENLTVIDNCATRLRLQVKDAGQVNEAALKRAGAKGVMKLS
NTSVQVIVGTNVESVADDMKKHV
>Mature_503_residues
MIGGIKMLQFLQRIGKALMLPIAVLPAAGLLLRLGQEDVFNIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAG
AIGYLVLQNTTNALSKTYSAAELNDKLKSVQDLVGSVDPTKLADTMTKVSKAAALTPKINMAILGGIIAGVVAGLLYNKF
HKIKLPEWLGFFAGKRFVPIITSIVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGAGLFGLLNRLLIPIGLHHVMNT
YFWFVLGDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLGGLALTSFLTG
ITEPIEFSFMFLSPVLYGIHAVLTGLSLFITTTLGIHDGFSFSAGAIDYVLNFGIATKPLLLAGIGLIYAAIYFVVFYFL
IKKFDLKTPGREDEEEVAEGEEAPVAGSIGETYVAALGGKENLTVIDNCATRLRLQVKDAGQVNEAALKRAGAKGVMKLS
NTSVQVIVGTNVESVADDMKKHV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=507, Percent_Identity=52.2682445759369, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1787343, Length=502, Percent_Identity=43.2270916334661, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787908, Length=538, Percent_Identity=36.4312267657993, Blast_Score=298, Evalue=6e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 53138; Mature: 53138

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure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CCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]