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The map label for this gene is dacA [H]

Identifier: 117919440

GI number: 117919440

Start: 1160999

End: 1162174

Strand: Reverse

Name: dacA [H]

Synonym: Shewana3_0991

Alternate gene names: 117919440

Gene position: 1162174-1160999 (Counterclockwise)

Preceding gene: 117919441

Following gene: 117919439

Centisome position: 23.37

GC content: 47.02

Gene sequence:

>1176_bases
ATGATGAATTTTGTAAAAAGTCCCATTAAAACATTGCTGCTAATTTCGAGTGTTTCTCTTCCTGTTTATGCTGCGCAGCC
GATTGTTACCCCTGATGCACCTACAGTTGCCGCCAAGGCTTACGTCTTGATGGATTATTACTCGGGCCAAATCATTGCCG
AAGAAAATGCCTACGAAAGCTTAAACCCAGCCAGCTTAACGAAGATGATGACCAGTTACGTGATTGGTCAAGAAATCAAA
GCCGGTAACGTATCCCCAGATGATGATGTGACCATCAGTAAAAATGCGTGGTCGAAGAACTTCTCTGACTCATCAAAAAT
GTTCATCGAAGTGGGTAAAACCGTTAAGGTTTCCGACTTAAACCGCGGCATCATCATCCAGTCGGGTAACGATGCCTGTG
TGGCCATGGCCGAACACATTGCCGGCACCGAAGGCGCCTTCGTCGACATGATGAACTCATGGGCCAAGCAGCTTGGCATG
CGTGACAGCTACTTCGAAAACTCCCACGGTTTAGACTCAGAAAACCACAAATCCACTGCCTACGATATGGCGTTATTAGG
TGCTGCGCTTATCCGTGATGTGCCTGAAGAATACCGTGTCTACAGCGAAAAATATTATACCTTCAACGGTATCAAGCAAT
ATAACCGCAACGGCCTGCTGTGGGATAACAGCATGAATGTCGACGGTATTAAAACCGGCCACACCTCTGGCGCGGGTTAT
AACTTAGTCGCATCTGCTACCAAAGACGGCATGCGTTTAATTTCAGTGGTAATGGGCACCCAGAGTGAAGCCGCCCGTAA
AGCCGAAAGTAAAAAGCTGCTTACCTATGGTTTCCGCTTCTTCGAAACCGTAACCCCTTACAAGGCGGGCGACAGCTTCG
TGACCCAACAAATTTGGTACGGCGATAAGAGCACTGTCGATTTAGGTGTTGCTACTGATACCCCAATAACCATCAGCCGT
GGCCGTGCGAAAGACTTAAAAGCCAACTTCGAACTGACTAAGCCACTGGATGCACCACTGAAAAAAGGTGAAACCGTTGG
CCGCTTATACTTCCAGTTAGATGGCAAAGATATTGCGCAATTCCCGCTGGTTACCCTGCAAGAAGTCAATGAAGGCAGCT
GGTTCAGCAAATTAGTTGATTACTTTAAGCAACTGTTTTCTGGTTGGTTTAGCTAA

Upstream 100 bases:

>100_bases
GATGAAGGAACCTTCACTGAAAACCTTTGTCGAAGGGTGGTATACTCGCGAAACTATTTTGCCTTTTCTTTCAATACAAC
CAAAAAGACGTGCTAAGTTA

Downstream 100 bases:

>100_bases
TCGGAATCACAAAAACTAAGCCACCTTCGGGTGGCTTAGTTGTTTTTGGGGGAGTATCGCCCTTGAATCGGGTATAATCG
TCACCATATAGTTCCCATTA

Product: penicillin-binding protein 6

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYESLNPASLTKMMTSYVIGQEIK
AGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENSHGLDSENHKSTAYDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWYGDKSTVDLGVATDTPITISR
GRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS

Sequences:

>Translated_391_residues
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYESLNPASLTKMMTSYVIGQEIK
AGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENSHGLDSENHKSTAYDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWYGDKSTVDLGVATDTPITISR
GRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS
>Mature_391_residues
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYESLNPASLTKMMTSYVIGQEIK
AGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDLNRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGM
RDSYFENSHGLDSENHKSTAYDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWYGDKSTVDLGVATDTPITISR
GRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQFPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=366, Percent_Identity=57.103825136612, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1787062, Length=377, Percent_Identity=54.3766578249337, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI87082022, Length=367, Percent_Identity=47.6839237057221, Blast_Score=353, Evalue=9e-99,
Organism=Escherichia coli, GI87082054, Length=255, Percent_Identity=26.2745098039216, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43210; Mature: 43210

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYES
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHEEEEEEECCCEEEECCCCCCC
LNPASLTKMMTSYVIGQEIKAGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEECC
NRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGMRDSYFENSHGLDSENHKSTA
CCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
HHHHHHHHHHHHHCCHHHHHHHHHEEEECCHHHHCCCCEEECCCCCCCCEECCCCCCCCC
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWY
EEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEC
GDKSTVDLGVATDTPITISRGRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQ
CCCCCEEEEEECCCCEEEECCCCCCCCCCCEECCCCCCHHHCCCCEEEEEEEECCCCHHH
FPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYES
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHEEEEEEECCCEEEECCCCCCC
LNPASLTKMMTSYVIGQEIKAGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEECC
NRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGMRDSYFENSHGLDSENHKSTA
CCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
YDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
HHHHHHHHHHHHHCCHHHHHHHHHEEEECCHHHHCCCCEEECCCCCCCCEECCCCCCCCC
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWY
EEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEEC
GDKSTVDLGVATDTPITISRGRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQ
CCCCCEEEEEECCCCEEEECCCCCCCCCCCEECCCCCCHHHCCCCEEEEEEEECCCCHHH
FPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]