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The map label for this gene is prfA [H]

Identifier: 117919221

GI number: 117919221

Start: 891616

End: 892707

Strand: Direct

Name: prfA [H]

Synonym: Shewana3_0769

Alternate gene names: 117919221

Gene position: 891616-892707 (Clockwise)

Preceding gene: 117919220

Following gene: 117919222

Centisome position: 17.93

GC content: 51.92

Gene sequence:

>1092_bases
ATGAAGGAATCCGTTATCCGCAAGCTGGAAGGCTTGCTCGAGCGCAATGAAGAAGTCTTAGCCTTGCTTGGTGATGCGTC
CGTTATCGCCGATCAGGAGCGTTTTCGCGCGTTATCCAAAGAATATTCTCAGTTAGAAGAAGTGGTTGCAGGCTTTAAAG
CCTACCAGCAAGCACAGGCGGATCTTGAGTCTGCAAAGGAAATGCTGGAAGAAGATGACGCTGAAATGCGCGAAATGGCG
CAGGAAGAAATCAAGGCAGCTAAAGCTGAACTTGAACGTCTCGAAGCCGAGCTGCAAATTCTGTTATTACCAAAAGATCC
TAACGACGACACCAACGCCTTTATCGAAATTCGTGCCGGTGCGGGTGGCGACGAAGCCGCAATCTTCGCGGGTGATCTGT
TCCGTATGTATAGCCGTTATGCCGAAGCCAACCGTTGGCAGCTCGAAATCATGAGCTCGAACGAAGGCGAACACGGTGGC
TTTAAAGAAATCATCGTTAAAGTCAGTGGTGAAGGTGCCTACGGTAAACTCAAGTTTGAATCCGGCGGTCACCGTGTTCA
ACGTGTGCCTGAAACTGAATCTCAAGGCCGTGTACACACCTCTGCCGTGACTGTGGTAGTCATGCACGAAGTGCCTGAGG
CGGAAGCGATTTCAATCAACCCAGCCGATCTGAAGGTCGATACCTTCCGTTCATCGGGCGCGGGTGGTCAGCACGTTAAC
AAAACCGATTCTGCCATTCGTATTACCCACATTCCAACCGGGATTGTGGTGGAATGTCAGGATCAACGTTCACAGCATAA
AAACCGTGCCCAAGCGATGAGCGTGTTAGCGGCGCGTATCCAAGCCGTTGAAGACGAAAAGCGCCGCAGCGCCGAAGAGT
CGACTCGCCGTAGTCTGGTAGCCAGTGGCGACCGCTCCGAGCGTGTGCGTACCTATAACTTCCCACAGGGCCGCGTGAGT
GAACACCGTATCAACTTAACCTTGTATCGCTTAAACGAAGTGATGGAAGGTGACTTAGATGCGATTTTAGGACCGCTGAT
GCAAGAGCATCAGGCCGACCTATTAGCCGCTCTGGCTGATGAGCAGGGGTAA

Upstream 100 bases:

>100_bases
CCCAAGCCCTCACAGTGGCGAGTCGTCAGGGTGATTTGAATACCTTAGGTCAGTTAAGAACAGCGCTCGGATTAGATAAA
AACTAAGGTTAGCGAGTTAA

Downstream 100 bases:

>100_bases
TTTTGGCTGATCAATCGAGTATCGCCGAGGCCCTGCAATGGGCCTATGTTCAGCTAGCGCCTACCTCTGAATCCGCCCAT
CTGGATGCAGAGGTTTTCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESVIRKLEGLLERNEEVLALLGDASVIADQERFRALSKEYSQLEEVVAGFKAYQQAQADLESAKEMLEEDDAEMREMA
QEEIKAAKAELERLEAELQILLLPKDPNDDTNAFIEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQLEIMSSNEGEHGG
FKEIIVKVSGEGAYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVMHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILGPLMQEHQADLLAALADEQG

Sequences:

>Translated_363_residues
MKESVIRKLEGLLERNEEVLALLGDASVIADQERFRALSKEYSQLEEVVAGFKAYQQAQADLESAKEMLEEDDAEMREMA
QEEIKAAKAELERLEAELQILLLPKDPNDDTNAFIEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQLEIMSSNEGEHGG
FKEIIVKVSGEGAYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVMHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILGPLMQEHQADLLAALADEQG
>Mature_363_residues
MKESVIRKLEGLLERNEEVLALLGDASVIADQERFRALSKEYSQLEEVVAGFKAYQQAQADLESAKEMLEEDDAEMREMA
QEEIKAAKAELERLEAELQILLLPKDPNDDTNAFIEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQLEIMSSNEGEHGG
FKEIIVKVSGEGAYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVMHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILGPLMQEHQADLLAALADEQG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=42.8082191780822, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=38.6167146974063, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=41.5458937198068, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=71.2707182320442, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=39.7014925373134, Blast_Score=221, Evalue=4e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=287, Percent_Identity=36.5853658536585, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=41.85303514377, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=40.785498489426, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40331; Mature: 40331

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESVIRKLEGLLERNEEVLALLGDASVIADQERFRALSKEYSQLEEVVAGFKAYQQAQA
CCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLESAKEMLEEDDAEMREMAQEEIKAAKAELERLEAELQILLLPKDPNDDTNAFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQLEIMSSNEGEHGGFKEIIVKVSGEGAYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVMHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEHHEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILGPLMQEHQADLLAALAD
HCCCCCCCCEEECCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
EQG
CCC
>Mature Secondary Structure
MKESVIRKLEGLLERNEEVLALLGDASVIADQERFRALSKEYSQLEEVVAGFKAYQQAQA
CCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLESAKEMLEEDDAEMREMAQEEIKAAKAELERLEAELQILLLPKDPNDDTNAFIEIRAG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQLEIMSSNEGEHGGFKEIIVKVSGEGAYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVMHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEHHEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILGPLMQEHQADLLAALAD
HCCCCCCCCEEECCCCCCCCCCEEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
EQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA