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The map label for this gene is hemK [H]

Identifier: 117919222

GI number: 117919222

Start: 892710

End: 893570

Strand: Direct

Name: hemK [H]

Synonym: Shewana3_0770

Alternate gene names: 117919222

Gene position: 892710-893570 (Clockwise)

Preceding gene: 117919221

Following gene: 117919224

Centisome position: 17.95

GC content: 51.92

Gene sequence:

>861_bases
TTGGCTGATCAATCGAGTATCGCCGAGGCCCTGCAATGGGCCTATGTTCAGCTAGCGCCTACCTCTGAATCCGCCCATCT
GGATGCAGAGGTTTTCCTGCTGTATTGCCTCAATAAAAACCGTGCATTTCTCTACACTTGGCCCGAAAAAGCCTTGACGG
TGGAGCAGTGGAAACGTTTCCAGCAAATGGTGCAACGTCGCCAGCAGGGCGTACCCGTGGCACATATTGTGGGCGAGCGT
GAGTTTTGGTCGCTGCCCTTTATCGTGAATGACACCACGTTAATCCCTCGCCCAGATACCGAAATCCTGGTTGAAACCGC
CTTAAACTTGCCGCTTGAGAGCAATGCTAAGGTGCTGGATTTAGGAACTGGAACCGGTGCTATTGCGCTGGCGTTGGCTT
CTGAGCGAGCGACTTGGCAAATCACCGCCGTCGATAAGGTGGAAGATGCAGTGGCATTGGCCAAAGCGAACCGGACTAAC
CTTAAGCTCGAGCAGGTGGAGATCCTCCAAAGTGACTGGTTTAGCGCTATCAAGGCCCACGACTTTGATTTAATTGTCTC
AAATCCGCCTTACATCGATGAGGCCGATGAGCATTTACATCAAGGGGATGTGCGCTTCGAGCCGCAAAGTGCGCTCACCG
CCGCCGATGAGGGTTTTGCCGATCTCTACTACATCGCAAAAACTGCTCGGGATTATCTGAAGCCCAACGGCTATATTCTG
CTCGAACACGGATTTGAGCAGGCGGTAAAGCTCAGAGAAAAACTGACCGAGCTGGGTTATCAAAATGTCGCGACCGTGCG
GGATTTTGGCAGCAACGACAGATGCACTATGGGTAAGTGGATGGGATTGATTGGAATTTAA

Upstream 100 bases:

>100_bases
ACGAAGTGATGGAAGGTGACTTAGATGCGATTTTAGGACCGCTGATGCAAGAGCATCAGGCCGACCTATTAGCCGCTCTG
GCTGATGAGCAGGGGTAATT

Downstream 100 bases:

>100_bases
TATTTAATCCAATCCCGCAAAGCACAAAACAACGCCATTGGTTATTTATCAATGGCGTTTTTGTTTGTGTATTGGTTGAA
ATGGTTAGCTTTTGACAAAC

Product: HemK family modification methylase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRFQQMVQRRQQGVPVAHIVGER
EFWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLDLGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTN
LKLEQVEILQSDWFSAIKAHDFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYIL
LEHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI

Sequences:

>Translated_286_residues
MADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRFQQMVQRRQQGVPVAHIVGER
EFWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLDLGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTN
LKLEQVEILQSDWFSAIKAHDFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYIL
LEHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI
>Mature_285_residues
ADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRFQQMVQRRQQGVPVAHIVGERE
FWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLDLGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTNL
KLEQVEILQSDWFSAIKAHDFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYILL
EHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=271, Percent_Identity=30.9963099630996, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1787463, Length=259, Percent_Identity=51.7374517374517, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082085, Length=208, Percent_Identity=29.8076923076923, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=203, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582226, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32169; Mature: 32038

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRF
CCCHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCHHCCHHHHHHH
QQMVQRRQQGVPVAHIVGEREFWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLD
HHHHHHHHCCCCEEEEECCCHHEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEE
LGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTNLKLEQVEILQSDWFSAIKAH
ECCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYIL
CEEEEECCCCCCCCCHHHHHCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LEHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI
EECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEHHHHHHHCCC
>Mature Secondary Structure 
ADQSSIAEALQWAYVQLAPTSESAHLDAEVFLLYCLNKNRAFLYTWPEKALTVEQWKRF
CCHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCHHCCHHHHHHH
QQMVQRRQQGVPVAHIVGEREFWSLPFIVNDTTLIPRPDTEILVETALNLPLESNAKVLD
HHHHHHHHCCCCEEEEECCCHHEECCEEECCCEECCCCCHHHHHHHHHCCCCCCCCEEEE
LGTGTGAIALALASERATWQITAVDKVEDAVALAKANRTNLKLEQVEILQSDWFSAIKAH
ECCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
DFDLIVSNPPYIDEADEHLHQGDVRFEPQSALTAADEGFADLYYIAKTARDYLKPNGYIL
CEEEEECCCCCCCCCHHHHHCCCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEE
LEHGFEQAVKLREKLTELGYQNVATVRDFGSNDRCTMGKWMGLIGI
EECCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]